30,535 results

1. PE-SUMO CRYBB1

   Plasmid

   #217675

   Purpose

   For expression of SUMO tagged human beta B1 crystallin

   Depositor

   Larry David

   Insert

   CRYBB1 (CRYBB1 Human)
   

   Use

   Tags

   6XHis and Smt3

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   May 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. Xla.Tubb2:hArr-TEV;he1.1:eYFP

   Plasmid

   #218859

   Purpose

   Xla.Tubb2 promoter driving expression of human Arrestin-TEV protease fusion protein for use in trans-Tango.

   Depositor

   Marnie Halpern

   Article

   Coomer et al Nat Neurosci. 2025 Jan;28(1):189-200. do

   Insert

   hArr
   

   Use

   Tags

   human Arrestin-Tobbaco Etch Virus protease fusion…

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Dec. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. MP28444 – pFUSE2ss-aRD114_CHIg-mIgG2A

   Plasmid

   #110555

   Purpose

   pFUSE backbone with an IL2 signal sequence, expressing the variable heavy chain of anti-RD114 binder fused to murine IgG2A constant heavy chain.

   Depositor

   Martin Pule

   Article

   Mekkaoui et al J Gen Virol. 2019 Nov 8. doi: 10.1099/jg

   Insert

   Anti-RD114 heavy chain
   

   Use

   Tags

   mIgG2A

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   EF-1 alpha core promoter

   Available Since

   Aug. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. T777T

   Plasmid

   #113082

   Purpose

   Enhanced RNAi vektor for C. elegans. Contains two T7 polymerase termination sequences adjacent to the T7 promoter sites and a lacZ coding sequence.

   Depositor

   Tibor Vellai

   Article

   Sturm et al Nucleic Acids Res. 2018 Jun 19. pii: 504

   Type

   Empty backbone

   Use

   RNAi

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Sept. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pUC18-mini-Tn7T-Gm-lacZ

   Plasmid

   #63120

   Purpose

   mini-Tn7 lacZ transcriptional fusion vector

   Depositor

   Herbert Schweizer

   Article

   Choi et al Nat Methods. 2005 Jun;2(6):443-8.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   May 17, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pYTK-DN4

   Plasmid

   #180285

   Purpose

   KanR multi-gene yeast expression vector (GFP-dropout)

   Depositor

   Andreas Milias-Argeitis

   Article

   Novarina et al STAR Protoc. 2022 Jun 10;3(2):101358. do

   Insert

   ConLS' - GFP - ConRE' - KanR - CEN6/ARS4
   

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available Since

   June 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGEX-4T1 SHP2 WT

   Plasmid

   #8322

   Depositor

   Ben Neel

   Article

   O'Reilly et al Mol Cell Biol 2000 Jan;20(1):299-311.

   Insert

   SHP2 (PTPN11 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   March 14, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBADmod1-linker2-gamS

   Plasmid

   #45833

   Depositor

   Richard Murray, Vincent Noireaux

   Article

   Sun et al ACS Synth Biol. 2014 Jun 20;3(6):387-97.

   Insert

   gamS
   

   Use

   Synthetic Biology

   Tags

   Histag

   Expression

   Bacterial

   Mutation

   Promoter

   pBAD

   Available Since

   May 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. MP28445 – pFUSE2ss-aRD114_CLIg-mIgK

   Plasmid

   #110556

   Purpose

   pFUSE backbone with an IL2 signal sequence, expressing the variable light chain of anti-RD114 binder fused to murine Kappa constant light chain.

   Depositor

   Martin Pule

   Article

   Mekkaoui et al J Gen Virol. 2019 Nov 8. doi: 10.1099/jg

   Insert

   anit-RD114 light chain
   

   Use

   Tags

   mKappa

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   EF-1 alpha core promoter

   Available Since

   Aug. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDD120

    Plasmid

    #119879

    Purpose

    Constitutive expression of Che9c60 and Che9c61 recombinases on pB264/pBR322 backbone with kanamycin resistance.

    Depositor

    Tae Seok Moon

    Article

    DeLorenzo et al ACS Synth Biol. 2018 Feb 16;7(2):727-738

    Insert

    pConstitutive_Che9c60 and Che9c61
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Feb. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pQTEV- PRDX2

    Plasmid

    #31338

    Depositor

    Konrad Buessow

    Article

    Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

    Insert

    PRDX2 (PRDX2 Human)
    

    Use

    Tags

    His and TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 22, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMSP1E3D1_D73C

    Plasmid

    #39328

    Depositor

    Stephen Sligar

    Article

    Ritchie et al Methods Enzymol. 2009;464:211-31. doi: 1

    Insert

    MSP1E3D1_D73C (APOA1 synthetic gene)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    cys mutant of MSP1E3D1

    Promoter

    T7

    Available Since

    Sept. 11, 2012

    Availability

    Academic Institutions and Nonprofits only

13. pLumi-LgBiT

    Plasmid

    #188319

    Purpose

    Expression of LgBiT via sigma 70 promoter in E. coli

    Depositor

    Kate Adamala

    Article

    Sato et al Sci Rep. 2022 Jul 7;12(1):11489. doi: 10

    Insert

    LgBiT
    

    Use

    Luciferase and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    sigma 70 promoter

    Available Since

    Sept. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. FLAG-MATR3

    Plasmid

    #32880

    Depositor

    Yossi Shiloh

    Article

    Salton et al PLoS One. 2011;6(8):e23882. Epub 2011 Au

    Insert

    MATR3 Matrin3 (MATR3 Human)
    

    Use

    Tags

    FLAG

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 6, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGEX-6P-1 Stu2 1-590

    Plasmid

    #38314

    Depositor

    Anthony Hyman

    Article

    Widlund et al Mol Biol Cell. 2012 Sep 19.

    Insert

    5' 1770 bp from STU2 ORF (STU2 Budding Yeast)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available Since

    Aug. 17, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET21a-Streptavidin-Dead

    Plasmid

    #20859

    Purpose

    Dead subunit for creating monovalent streptavidin with a single femtomolar biotin binding site. Use in combination with Streptavidin-Alive (construct 20860).

    Depositor

    Alice Ting

    Article

    Howarth et al Nat Methods. 2006 Apr . 3(4):267-73.

    Insert

    Streptavidin-Dead
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Triple mutant of wt Streptavidin N23A,S27D,S45A

    Promoter

    Available Since

    April 21, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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17. pUC18T-mini-Tn7T-Gm

    Plasmid

    #63121

    Purpose

    mini-Tn7 vector

    Depositor

    Herbert Schweizer

    Article

    Choi et al Nat Methods. 2005 Jun;2(6):443-8.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    May 17, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

18. pTn7CdrA-gfp(ASV)C

    Plasmid

    #111617

    Purpose

    Fluorescence-based gauging of the level of the nucleotide secondary messenger cyclic di-GMP in Pseudomonas aeruginosa and related species.

    Depositor

    Tim Tolker-Nielsen

    Article

    Rybtke et al Appl Environ Microbiol. 2012 Aug;78(15):

    Insert

    PcdrA-RBSII-gfp(ASV)-T0-T1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    June 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBHRSF184

    Plasmid

    #89482

    Purpose

    Express asparaginyl endopeptidase 1 from O. affinis (OaAEP1)

    Depositor

    Hideo Iwai

    Article

    Mikula et al FEBS Lett. 2017 Mar 30. doi: 10.1002/187

    Insert

    Asparaginyl endopeptidase from Oldenlandia affinis
    

    Use

    Tags

    Smt3

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    June 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. luxB:cp157Venus+luxA/pRSETB Duet

    Plasmid

    #197583

    Purpose

    BRET lux luciferase with cp157Venus for bacterial expression

    Depositor

    Takeharu Nagai

    Article

    Kaku et al Sci Rep. 2021 Jul 22;11(1):14994. doi: 1

    Insert

    LuxA LuxBcp157Venus
    

    Use

    E.coli expression

    Tags

    His X 6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    April 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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