30,258 results

1. pDXinit

   Plasmid

   #110101

   Purpose

   E.coli entry and expression vector for FX cloning system, N-terminal pelB signal sequence and C-terminal fusion to PIII for phage display using M13 phages

   Depositor

   Markus Seeger

   Article

   Zimmermann et al Elife. 2018 May 24;7. pii: 34317. doi: 1

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pComb8

   Plasmid

   #63889

   Purpose

   For phage display of antibody libraries by fusion to modified geneVIII.

   Depositor

   Carlos Barbas

   Article

   Barbas CF et al Proc Natl Acad Sci U S A. 1991 Sep 15;88

   Type

   Empty backbone

   Use

   Phage display

   Tags

   geneVIII

   Expression

   Bacterial

   Mutation

   Promoter

   lacZ

   Available since

   April 29, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGNW2

   Plasmid

   #122086

   Purpose

   suicide vector for integration into Pseudomonas genome after insertion of homologous regions at the multiple cloning site; successful integration causes green fluorescence in cells

   Depositor

   Pablo Ivan Nikel

   Article

   Wirth et al Microb Biotechnol. 2019 Mar 12. doi: 10.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. I48 ELP

   Plasmid

   #68937

   Purpose

   Elastin-like polypeptide (VPGxG) with 48 repeats of Isoleucine guest residue

   Depositor

   Andrew MacKay

   Article

   Shi et al J Control Release. 2013 Nov 10;171(3):33

   Insert

   I48 ELP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 1, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMYC-GFP

   Plasmid

   #42142

   Depositor

   Olufunmilayo Olopade

   Article

   Chen et al BMC Cancer. 2011 Jun 13;11:246. doi: 10.

   Insert

   c-Myc (MYC Human)
   

   Use

   Tags

   GFP

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 31, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. IL13 promoter luciferase

   Plasmid

   #11784

   Depositor

   Anjana Rao

   Article

   Macian et al EMBO J. 2000 Sep 1. 19(17):4783-95.

   Insert

   IL-13 promoter (IL13 Human)
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 4, 2006

   Availability

   Academic Institutions and Nonprofits only

7. MP4

   Plasmid

   #69652

   Purpose

   Untargetted Bacterial Mutagenesis

   Depositor

   David Liu

   Article

   Badran et al Nat Commun. 2015 Oct 7;6:8425. doi: 10.1

   Insert

   dnaQ926 dam seqA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   E. coli PBAD

   Available since

   Oct. 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. TGT-His

   Plasmid

   #138201

   Purpose

   Expresses E. Coli TGT with a C-terminal His Tag

   Depositor

   Neal Devaraj

   Article

   Busby et al Methods Enzymol. 2020;641:373-399. doi:

   Insert

   E Coli tRNA Guanine Transglycosylase-His
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   Feb. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pUA66 PcspA GFP No Linker

   Plasmid

   #105605

   Purpose

   Expresses GFP under temperature sensitive promoter PcspA

   Depositor

   Pamela Silver

   Article

   Stirling et al Mol Cell. 2017 Nov 16;68(4):686-697.e3.

   Insert

   GFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PcspA

   Available since

   Feb. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET21-pelB-LaG94-15

    Plasmid

    #228971

    Purpose

    For bacterial expression of anti-GFP nanobody LaG94-15, with pelB leader for periplasmic secretion, and C-term free cysteine and 6xHIS tag.

    Depositor

    Michael Rout

    Article

    Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

    Insert

    LaG94-15
    

    Use

    Tags

    6xHIS

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pET21-pelB-LaMIgG-14

    Plasmid

    #228994

    Purpose

    For bacterial expression of anti-mouse IgG nanobody LaMIgG-14, with pelB leader for periplasmic secretion, and C-term free cysteine and C-term 6xHIS tag.

    Depositor

    Michael Rout

    Article

    Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

    Insert

    LaMIgG-14
    

    Use

    Tags

    6xHIS

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET21-pelB-LaTdT-39

    Plasmid

    #228980

    Purpose

    For bacterial expression of anti-tdTomato nanobody LaTdT-39, with pelB leader for periplasmic secretion, and C-term 6xHIS tag.

    Depositor

    Michael Rout

    Article

    Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

    Insert

    LaTdT-39
    

    Use

    Tags

    6xHIS

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pUCSh5vio-2300

    Plasmid

    #170987

    Purpose

    Encodes sgRNA target 2300 and Gentamicin resistance - violacein pathway transposon.

    Depositor

    Christopher Reisch

    Article

    Trujillo Rodriguez et al ACS Synth Biol. 2023 Jun 27. doi: 10.102

    Insert

    Sh-CAST sgRNA 2300 violacein
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    placI

    Available since

    July 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pNCS-mClover3

    Plasmid

    #74236

    Purpose

    Constitutive expression of mClover3 in bacteria

    Depositor

    Michael Lin

    Article

    Bajar et al Sci Rep. 2016 Feb 16;6:20889. doi: 10.10

    Has Service

    Cloning Grade DNA

    Insert

    mClover3
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pETduet+sfGFP-TetR-p300HAT

    Plasmid

    #157795

    Purpose

    Expression of sfGFP-TetR-p300HAT fusion protein

    Depositor

    Michael Rosen

    Article

    Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

    Insert

    sfGFP-TetR-p300 (EP300 Human)
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pALS3-Ma-sfGFP-WT

    Plasmid

    #212119

    Purpose

    Fluorescence control plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses sfGFP-WT and M. alvus Pyl-tRNA(6). p15a origin. Tetracycline resistance.

    Depositor

    Ryan Mehl

    Inserts

    SUMO-sfGFP-WT
    M. alvus Pyl-tRNA(6)
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    araC and lpp

    Available since

    Sept. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV-Spike-VLP

    Plasmid

    #173922

    Purpose

    Expresses virus-like particles based upon the original SARS-Cov-2

    Depositor

    Andreas Stuermer

    Inserts

    SARS-2 Spike Protein (S Human, SARS-COV-2, Synthetic)
    SARS-Cov-2 E protein (E Human, SARS-COV-2, Synthetic)
    SARS-Cov-2 M Protein (M Human, SARS-COV-2, Synthetic)
    

    Use

    Tags

    no tags, native protein

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    22bp IRES, CMV, and IE2 Promoter/Enhancer from Mo…

    Available since

    April 11, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. pMJ806

    Plasmid

    #39312

    Depositor

    Jennifer Doudna

    Article

    Jinek et al Science. 2012 Aug 17;337(6096):816-21. d

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    His6 and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 7, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGEX Emt-SH2

    Plasmid

    #46424

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    Emt (ITK Human)
    

    Use

    Tags

    GST and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 223-346

    Promoter

    Tac

    Available since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET26b_AT118-L-V5-His

    Plasmid

    #222848

    Purpose

    E. coli periplasmid expression plasmid encoding Nb. AT118-L, a humanized nanobody antagonist of the human Angiotensin II type I receptor (AT1R) with reduced polyreactivity

    Depositor

    Andrew Kruse

    Article

    Skiba et al Nat Chem Biol. 2024 May 14. doi: 10.1038

    Insert

    AT118-L
    

    Use

    Tags

    V5-His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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