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pHyo245

Plasmid

#173147

Purpose

Expresses pheS_A294G by dual T7 promoter in e.coli

Depositor

Seokhee Kim

Article

Park et al Nucleic Acids Res. 2021 Apr 6;49(6):e32.

Insert

pheS_A294G

Expression

Bacterial

Available Since

Sept. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pETduet+p300HAT

Plasmid

#157793

Purpose

Expression of p300 HAT domain

Depositor

Michael Rosen

Article

Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

Insert

p300HAT (EP300 Human)

Tags

His6

Expression

Bacterial

Promoter

T7

Available Since

Aug. 5, 2020

Availability

Academic Institutions and Nonprofits only

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pAWP78-PVCpnf1-16

Plasmid

#198271

Purpose

Unmodified pPVC; contains WT PVC structural/accessory genes

Depositor

Feng Zhang

Article

Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

Insert

PVCpnf1-16

Expression

Bacterial

Available Since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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pTP264

Plasmid

#149334

Purpose

Bacterial expression of Biotin ligase BirA

Depositor

Dirk Görlich

Article

Pleiner et al Elife. 2015 Dec 3;4:e11349. doi: 10.7554

Insert

Biotin ligase BirA

Tags

His14 and bdNEDD8

Expression

Bacterial

Available Since

June 18, 2020

Availability

Academic Institutions and Nonprofits only

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pExp-MBP

Plasmid

#112568

Purpose

Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and MBP fusions with optional C-terminal StrepII-tag

Depositor

Marko Hyvönen

Type

Empty backbone

Tags

8xHis-MBP and Strep tag

Expression

Bacterial

Available Since

Feb. 1, 2021

Availability

Academic Institutions and Nonprofits only

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LVXN-Neo-NSD2

Plasmid

#86010

Purpose

express wild-type NSD2 in mammalian cells

Depositor

Darrin Stuart

Article

Jaffe et al Nat Genet. 2013 Nov;45(11):1386-91. doi:

Insert

NSD2 (NSD2 Human)

Use

Lentiviral

Tags

Flag tag D Y K D D D D K

Expression

Bacterial

Mutation

K1002R (please see depositor comments below)

Promoter

CMV

Available Since

Jan. 11, 2017

Availability

Academic Institutions and Nonprofits only

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pET-15b-TEV-C9R

Plasmid

#118754

Purpose

TEV-C9R protease expression vector with enhanced expression, solubility and yield without affecting TEV's protease activity

Depositor

Yutaka Kuroda

Article

Nautiyal et al N Biotechnol. 2018 May 25;42:77-84. doi:

Insert

Tobacco Etch Virus protease (NEWENTRY Synthetic)

Tags

C9R and His tag

Expression

Bacterial

Promoter

T7

Available Since

Jan. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pSUC01

Plasmid

#177204

Purpose

chromogenic reporter for gene knockout in Streptomyces

Depositor

Wenjun Guan

Article

Dong et al J Microbiol Biotechnol. 2021 Dec 28;31(1

Insert

SaindC

Use

Synthetic Biology

Tags

NONE

Expression

Bacterial

Mutation

NONE

Promoter

TGTTCACATTCGAACGGTCTCTGCTTTGACAACATGCTGTGCGGTGTTG…

Available Since

Jan. 19, 2023

Availability

Academic Institutions and Nonprofits only

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pFW2000

Plasmid

#224211

Purpose

E. coli-Bacteroides shuttle plasmid; pB8-51 (Bacteroides replication origin), p15A (E. coli replication origin)

Depositor

Ophelia Venturelli

Article

Feng et al Cell Host Microbe. 2022 Feb 9;30(2):200-

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

Sept. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pKD236-4b

Plasmid

#90956

Purpose

Expresses ThsS constitutively

Depositor

Jeffrey Tabor

Article

Daeffler et al Mol Syst Biol. 2017 Apr 3;13(4):923. doi

Insert

ThsS

Expression

Bacterial

Promoter

Bba_J23104

Available Since

May 16, 2017

Availability

Academic Institutions and Nonprofits only

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FRESCA-syntide

Plasmid

#132415

Purpose

FRET-based sensor for CaMKII activity

Depositor

Margaret Stratton

Article

Ardestani et al J Biol Chem. 2019 Jun 14. pii: RA119.009

Insert

FRESCA-syntide

Expression

Bacterial and Mammalian

Promoter

CMV

Available Since

Oct. 29, 2019

Availability

Academic Institutions and Nonprofits only

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pGEX6P1-mCherry-Nanobody (LaM-2)

Plasmid

#162276

Purpose

Expresses GST-tagged anti-mCherry Nanobody (LaM-2) in E. coli cells.

Depositor

Kazuhisa Nakayama

Article

Ishida et al Hum Mol Genet. 2021 Apr 26;30(3-4):213-2

Insert

mCherry-Nanobody (LaM-2)

Tags

GST

Expression

Bacterial

Available Since

Feb. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pCON1-ProC.gfp

Plasmid

#112517

Purpose

ProC driven gfpmut3 expression with synTT

Depositor

Olivia Steele-Mortimer

Article

Cooper et al Front Cell Infect Microbiol. 2017 Nov 16

Insert

ProC gfpmut3 TT

Expression

Bacterial

Promoter

ProC

Available Since

July 17, 2018

Availability

Academic Institutions and Nonprofits only

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pKD237-3a-2

Plasmid

#90957

Purpose

Expresses ThsR and mCherry constitutively and sfGFP under the PphsA promoter

Depositor

Jeffrey Tabor

Article

Daeffler et al Mol Syst Biol. 2017 Apr 3;13(4):923. doi

Inserts

Thiosulfate response regulator
Superfolder GFP
mCherry

Expression

Bacterial

Promoter

Bba_J23100, Bba_J23105, and PphsA

Available Since

May 16, 2017

Availability

Academic Institutions and Nonprofits only

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pRSFDuet-1(nsp8-nsp7)(nsp12)

Plasmid

#165451

Purpose

Expresses the RNA Dependent RNA polymerase complex of SARS-CoV-2 in Escherichia Coli

Depositor

Marc Delarue

Article

Madru et al PLoS One. 2021 Apr 29;16(4):e0250610. do

Inserts

nsp12
nsp8
nsp7

Tags

14Histidine-TEV

Expression

Bacterial

Promoter

T7

Available Since

May 25, 2021

Availability

Academic Institutions and Nonprofits only

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pSK+KanaRpsL

Plasmid

#20871

Depositor

Jiyue Zhu

Article

Wang et al Mol Biotechnol. 2009 May;42(1):110-6. do

Insert

KanamycinResistance-RpsL

Expression

Bacterial

Mutation

none

Available Since

May 5, 2009

Availability

Academic Institutions and Nonprofits only

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pET His6 LIC cloning vector (2Bc-T)

Plasmid

#37236

Depositor

Scott Gradia

Type

Empty backbone

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Aug. 2, 2012

Availability

Academic Institutions and Nonprofits only

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pQE-60NA-msGFP2

Plasmid

#135301

Purpose

Encodes a photostable monomeric superfolder GFP

Depositor

Benjamin Glick

Article

Valbuena et al Traffic. 2020 May 15. doi: 10.1111/tra.1

Insert

msGFP2

Expression

Bacterial

Promoter

T5

Available Since

Jan. 15, 2020

Availability

Academic Institutions and Nonprofits only

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6xHis-MBP-BrCas12b-RFND

Plasmid

#195339

Purpose

Expresses Engineered BrCas12b in Bacteria

Depositor

Piyush Jain

Article

Nguyen et al Cell Rep Med. 2023 May 16;4(5):101037. d

Insert

BrCas12b

Use

CRISPR and Synthetic Biology

Tags

6xHis and 6xHis, MBP, TEV cleavage site

Expression

Bacterial

Mutation

R160E, F208W, N524V, D868V

Promoter

T7

Available Since

May 5, 2023

Availability

Academic Institutions and Nonprofits only

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pBBR1k-con-GFPuv

Plasmid

#106386

Purpose

Constitutive trc promoter expressing gfpuv on BBR1 origin

Depositor

Brian Pfleger

Article

Cook et al J Ind Microbiol Biotechnol. 2018 Jan 3.

Insert

GFPuv

Expression

Bacterial

Promoter

trc

Available Since

March 22, 2018

Availability

Academic Institutions and Nonprofits only

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