30,535 results

1. pCDB180

   Plasmid

   #80677

   Purpose

   for secretion of N-terminal OsmY, deca-histidine, SUMO tagged protein

   Depositor

   David Baker

   Article

   Bhardwaj et al Nature. 2016 Sep 14. doi: 10.1038/nature

   Type

   Empty backbone

   Use

   Tags

   OsmY-10His-Smt3

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Nov. 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pUCP20T-E2Crimson

   Plasmid

   #78473

   Purpose

   Broad range bacterial fluorescence labeling

   Depositor

   Mariette Barbier

   Article

   Barbier et al PLoS One. 2016 Mar 3;11(3):e0146827. doi

   Insert

   E2-Crimson
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   June 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET His6 MBP TEV LIC cloning vector (2M-T)

   Plasmid

   #29708

   Depositor

   Scott Gradia

   Type

   Empty backbone

   Use

   Tags

   6xHis, MBP, and TEV cleavage site

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Aug. 17, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBBR8k-GFPuv

   Plasmid

   #106385

   Purpose

   L-arabinose-inducible araBAD promoter expressing gfpuv on BBR1 origin

   Depositor

   Brian Pfleger

   Article

   Cook et al J Ind Microbiol Biotechnol. 2018 Jan 3.

   Insert

   GFPuv
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   araBAD

   Available Since

   March 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET28a-mH6-Cas12c1

   Plasmid

   #120872

   Purpose

   Expresses E. coli codon optimized Cas12c1 effector in the pET28a backbone.

   Depositor

   Arbor Biotechnologies

   Article

   Yan et al Science. 2019 Jan 4;363(6422):88-91. doi

   Insert

   Cas12c1
   

   Use

   CRISPR

   Tags

   mH6

   Expression

   Bacterial

   Mutation

   WT

   Promoter

   lac

   Available Since

   Feb. 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJExpress414_GmPOPB

   Plasmid

   #92234

   Purpose

   Express wild type prolyl oligopeptidase from Galerina marginata

   Depositor

   James Naismith

   Article

   Czekster et al Biochemistry. 2017 Apr 18;56(15):2086-20

   Insert

   Prolyl oligopeptidase B from Galerina marginata
   

   Use

   Tags

   6x-Histag, TEV cleavage site

   Expression

   Bacterial

   Mutation

   none

   Promoter

   T7 promoter

   Available Since

   Aug. 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pKS1

   Plasmid

   #84079

   Purpose

   Expresses the non-toxic fragment C of tetanus toxin

   Depositor

   Neil Fairweather

   Article

   Sinha et al Mol Microbiol. 2000 Sep;37(5):1041-51.

   Insert

   Tetanus neurotoxin fragment C
   

   Use

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   April 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. HMGCS1

   Plasmid

   #38844

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   HMGCS1 (HMGCS1 Human)
   

   Use

   Tags

   His Tev

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   March 18, 2013

   Availability

   Academic Institutions and Nonprofits only

9. Reb_79 aka PAS620

   Plasmid

   #71225

   Purpose

   Reb_1 with RebA(Q91R)

   Depositor

   Pamela Silver

   Article

   Polka et al ACS Synth Biol. 2016 Feb 3.

   Insert

   Reb locus
   

   Use

   Tags

   none

   Expression

   Bacterial

   Mutation

   RebA( G53A, Q91R)

   Promoter

   Available Since

   April 7, 2016

   Availability

   Academic Institutions and Nonprofits only

10. pJMD14

    Plasmid

    #138654

    Purpose

    phiOZJ integrating Streptomyces expression vector, intact RDF

    Depositor

    Joshua Blodgett

    Article

    Ko et al J Ind Microbiol Biotechnol. 2019 Nov 8.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PermE* variant sequence from pDA1652

    Available Since

    April 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. PKM2

    Plasmid

    #25538

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    PKM2 (PKM Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Aug. 24, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

12. pACYCDuet-1_Ec_rpoZ

    Plasmid

    #128837

    Purpose

    E. coli rpoz gene cloned between NcoI and HindIII sites of the first MCS of this Duet plasmid. Omega protein.

    Depositor

    Seth Darst

    Article

    Murakami et al J Biol Chem. 2013 Mar 29;288(13):9126-34

    Insert

    rpoZ (rpoZ E. coli)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Aug. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSm-Nuclease

    Plasmid

    #132431

    Purpose

    Expresses Serratia marcescens nuclease in E. coli using T7 promoter

    Depositor

    Mark Arbing

    Insert

    Serratia marcescens nuclease (codon optimized)
    

    Use

    Tags

    None

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Oct. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBbA5a-sufABCDSE

    Plasmid

    #195056

    Purpose

    Expresses SUF operon from E. coli from IPTG-inducible promoter

    Depositor

    Greg Bokinsky

    Article

    D'Angelo et al Elife. 2022 Mar 4;11:e70936. doi: 10.755

    Insert

    sufABCDSE
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Feb. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. AP1

    Plasmid

    #179678

    Purpose

    Accessory plasmids

    Depositor

    David Liu

    Article

    Mok et al Nat Biotechnol. 2022 Sep;40(9):1378-1387

    Insert

    [SD8] gIII
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    April 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAWP78-PVCpnf_Δpvc10

    Plasmid

    #198448

    Purpose

    Produces PVCs containing truncated spike tip protein (Pvc10).

    Depositor

    Feng Zhang

    Article

    Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

    Insert

    PVCpnf_Δpvc10
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMRE145

    Plasmid

    #118497

    Purpose

    broad-host range plasmid to constitutively express fluorescent protein genes in bacteria

    Depositor

    Mitja Remus-Emsermann

    Article

    Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

    Insert

    mScarlet-I
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Oct. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. 2Bc-T MBP_BoMoC(ed)_W403A_F753A_6xH

    Plasmid

    #185713

    Purpose

    Recombinant B. mori truncated R2 RT expression

    Depositor

    Kathleen Collins

    Article

    Pimentel et al J Biol Chem. 2022 Mar;298(3):101624. doi

    Insert

    B. mori truncated R2 RT
    

    Use

    Tags

    6xHis and MBP

    Expression

    Bacterial

    Mutation

    Changed W403 and F753 to A

    Promoter

    T7

    Available Since

    Aug. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLEM415-ldhL-mRFP1

    Plasmid

    #99842

    Purpose

    Expression of RFP in Lactobacillus

    Depositor

    Sujin Bao

    Article

    Bao et al PLoS One. 2013;8(3):e60007. doi: 10.1371

    Insert

    monomeric red fluorescent protein
    

    Use

    Tags

    FLAG

    Expression

    Bacterial

    Mutation

    Promoter

    ldhl

    Available Since

    Nov. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pZH81 pET21b Rat1-His

    Plasmid

    #231650

    Purpose

    Bacterial expression vector expressing yeast S. cerevisiae rat1 with a C-terminal his tag

    Depositor

    Jesper Svejstrup

    Article

    Han et al Mol Cell. 2023 Sep 21;83(18):3253-3267.e

    Insert

    Rat1 (RAT1 Budding Yeast)
    

    Use

    Tags

    6His

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Feb. 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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