30,535 results

1. pET32a-hEGF

   Plasmid

   #199231

   Purpose

   Bacterial production of soluble, active target proteins; Nterm thrombin and enterokinase cleavage sites.

   Depositor

   Nilson Zanchin

   Article

   Ferreira et al Sci Rep. 2022 Mar 23;12(1):5034. doi: 10

   Insert

   Epidermal growth factor, partial (EGF Human)
   

   Use

   Tags

   TrxA-6xHis-S-tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   May 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pKDsgRNA-NT

   Plasmid

   #89960

   Purpose

   Contains arabinose-induced lambda Red and a tet-inducible non-targeting gRNA containing BsmBI sites for cloning.

   Depositor

   Michael Lynch

   Article

   Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

   Insert

   empty gRNA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pTet

   Available Since

   June 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pASPIre2

   Plasmid

   #154843

   Purpose

   Derivative of pASPIre1 carrying a bxb1-sfGFP translational fusion in place of bxb1

   Depositor

   Markus Jeschek

   Article

   Hollerer et al Nat Commun. 2020 Jul 15;11(1):3551. doi:

   Insert

   Bxb1-sfGFP fusion controlled by rhamnose-inducible promoter; attB/attP-flanked discriminator containing mCherry; rhaRS operon
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Aug. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. HMBP-3C-STAR.66-285

   Plasmid

   #100094

   Purpose

   expresses MBP-tagged STARD1 protein domain (residues 66-285)

   Depositor

   Nikolai Sluchanko

   Article

   Sluchanko et al Protein Expr Purif. 2016 Mar;119:27-35.

   Insert

   STAR protein (STAR Human)
   

   Use

   Tags

   His-tagged MBP

   Expression

   Bacterial

   Mutation

   Promoter

   Ptac

   Available Since

   Sept. 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCN34e-ktrA-yfp

   Plasmid

   #227640

   Purpose

   c-di-AMP biosensor plasmid. Encodes yfp downstream of c-di-AMP-binding ktrA riboswitch. Plasmid confers ampicillin resistance to E. coli and erythromycin resistance to S. aureus.

   Depositor

   Angelika Grundling

   Article

   Kviatkovski et al mSphere. 2024 Sep 17:e0032124. doi: 10.1

   Insert

   ktrA-yfp
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Dec. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJYS2.1-crtYf

   Plasmid

   #89077

   Purpose

   Constitutive transcription of crRNA of crtYF. CRISPR of C. glutamicum.

   Depositor

   Sheng Yang

   Article

   Jiang et al Nat Commun. 2017 May 4;8:15179. doi: 10.

   Insert

   crRNA of crtYF
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   May 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET28a-mH6-Cas12i2

   Plasmid

   #120883

   Purpose

   Expresses E. coli codon optimized Cas12i2 effector in the pET28a backbone.

   Depositor

   Arbor Biotechnologies

   Article

   Yan et al Science. 2019 Jan 4;363(6422):88-91. doi

   Insert

   Cas12i2
   

   Use

   CRISPR

   Tags

   mH6

   Expression

   Bacterial

   Mutation

   WT

   Promoter

   lac

   Available Since

   Feb. 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCasY1

   Plasmid

   #87687

   Purpose

   p15A plasmid expressing CasY1

   Depositor

   Jennifer Doudna

   Article

   Burstein et al Nature. 2016 Dec 22. doi: 10.1038/nature

   Insert

   CasY1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   April 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. RpHLuorin2

   Plasmid

   #180227

   Purpose

   for bacterial expression of recombinant ratiometric pHluorin2

   Depositor

   Geert van den Bogaart

   Article

   Linders et al ACS Chem Biol. 2022 Jan 21;17(1):240-251

   Insert

   Ratiometric pHLuorin2
   

   Use

   Tags

   His-tag followed by thrombin cleavage site

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   March 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCVL.SSA TLR (Sce)

    Plasmid

    #45576

    Purpose

    Codes for the SSA-TLR with the target site for I-Sce I in a lentiviral backbone

    Depositor

    Andrew Scharenberg

    Article

    Kuhar et al Nucleic Acids Res. 2013 Oct 10.

    Inserts

    5' iRFP arm
    eGFP with I-Sce I TS
    +3 mCherry
    3' iRFP arm
    

    Use

    Lentiviral

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    embedded I-Sce I TS from 163-185bp, truncated 25 …

    Promoter

    SFFV

    Available Since

    Nov. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET15b-His-IkappaBalpha(FL)

    Plasmid

    #44738

    Depositor

    Gourisankar Ghosh

    Insert

    IkappaBalpga (Nfkbia Mouse)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    June 3, 2013

    Availability

    Academic Institutions and Nonprofits only

12. pCold-I-Dsup

    Plasmid

    #90021

    Purpose

    To express His-tagged Dsup in bacteria

    Depositor

    Takekazu Kunieda

    Article

    Hashimoto et al Nat Commun. 2016 Sep 20;7:12808. doi: 10

    Insert

    Dsup
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    cspA

    Available Since

    May 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET303_sumocodo_GFP_codoopti

    Plasmid

    #210393

    Purpose

    Express SUMO-GFP in E. coli

    Depositor

    Magnus Rueping

    Article

    Peng et al ACS Synth Biol. 2025 Jan 9. doi: 10.1021

    Insert

    GFP
    

    Use

    Tags

    SUMO-tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Jan. 13, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pQE30 PTBP1 isoform1

    Plasmid

    #108591

    Purpose

    Recombinant His tagged protein production

    Depositor

    Chris Smith

    Article

    Wollerton et al RNA. 2001 Jun;7(6):819-32.

    Insert

    PTBP1 isoform 1 (PTBP1 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    isoform 1 (isoform c)

    Promoter

    Available Since

    Dec. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEXP5-NT/6xHis mCherry F30 2xdBroccoli

    Plasmid

    #169233

    Purpose

    Constitutive T7 RNAP-mediated expression of 6xHis-mCherry with 2xd Broccoli RNA aptamer.

    Depositor

    Dora Tang

    Article

    Gonzales et al ACS Synth Biol. 2022 Jan 21;11(1):205-21

    Insert

    6xHis mCherry F30-2xdBroccoli
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available Since

    Dec. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAR17_StrepI_hFASh_H8_pET22b

    Plasmid

    #122846

    Purpose

    Expresses human type I fatty acid synthase (FASN) in Escherichia coli. N-terminal Twin-Strep tag; C-terminal H8-tag; in pET22b vector.

    Depositor

    Martin Grininger

    Article

    Rittner et al Protein Sci. 2019 Feb;28(2):414-428. doi

    Insert

    human FASN/ human fatty acid synthase (FASN Human)
    

    Use

    Tags

    His8 tag and Twin-Strep tag

    Expression

    Bacterial

    Mutation

    wildtype

    Promoter

    T7 promoter

    Available Since

    March 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. GST-GRP78-Full Length (pGEX4T1)

    Plasmid

    #238421

    Purpose

    Expresses GRP78 Full Length fused to the GST Tag

    Depositor

    Amy Shiu Lee

    Article

    Tseng et al Neoplasia. 2019 Aug;21(8):837-848. doi:

    Insert

    78-kDa glucose-regulated protein (HSPA5 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Aug. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. 73-21 KRAS PANCS-binder posAP

    Plasmid

    #240119

    Purpose

    KRAS G12D PANCS-binder positive AP

    Depositor

    Bryan Dickinson

    Article

    Styles et al bioRxiv [Preprint]. 2025 Jan 17:2025.01.

    Insert

    KRas-RNAPC Pos AP fusion
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    custom

    Available Since

    July 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pRPCN

    Plasmid

    #193726

    Purpose

    Expresses Cas9 from RK2 origin with RFP-marked sgRNA insertion site and kanR/sacB selection/counterselection

    Depositor

    Martin Buck

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Feb. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. His6-MBP-uTEV3

    Plasmid

    #135464

    Purpose

    Bacterial expression of uTEV3 (full-length) protease

    Depositor

    Alice Ting

    Article

    Sanchez et al Nat Methods. 2019 Dec 9. pii: 10.1038/s4

    Insert

    His6-MBP-uTEV3
    

    Use

    Tags

    MBP, His6

    Expression

    Bacterial

    Mutation

    S219V mutation (improves stability) , I138T/S153N…

    Promoter

    T5

    Available Since

    Jan. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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