30,736 results

1. lox2272 Neo* lox2272

   Plasmid

   #168769

   Purpose

   lox2272 (lox variant)-flanked pPgk-pEM7-Neo*-bpA positive selection cassette

   Depositor

   Mario Capecchi

   Insert

   Neo*
   

   Use

   Cre/Lox and Mouse Targeting

   Expression

   Bacterial and Mammalian

   Available Since

   July 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. SBFP2-anti-AlfaTag nanobody

   Plasmid

   #171826

   Purpose

   Bacterial expression vector for production of fluorescent anti-AlfaTag nanobody

   Depositor

   Ikuo Wada

   Article

   Matsumoto et al J Biol Chem. 2021 Jul;297(1):100851. doi

   Insert

   SBFP2-anti-AlfaTag nanobody
   

   Tags

   6xHis and SEP tag (6xD)

   Expression

   Bacterial

   Available Since

   July 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRSETA_crE6

   Plasmid

   #172928

   Purpose

   Crowding FRET sensor with mCerulean3 and mCitrine for E. coli expression. Linker comprises 1 helix and 2 GSG linkers

   Depositor

   Arnold Boersma

   Article

   Liu et al Biophys J. 2017 May 9;112(9):1929-1939.

   Insert

   CrowdingSensorE6
   

   Tags

   His

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pT7C-HSPA8

   Plasmid

   #177661

   Purpose

   Recombinant protein synthesis of human HSPA8

   Depositor

   Jeff Kuret

   Article

   Ayoub et al PLoS Genet. 2023 Mar 27;19(3):e1010681.

   Insert

   HSPA8 (HSPA8 Human)
   

   Tags

   6xHis-Thrombin

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Dec. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET51b(+)_SNAP-tag2

   Plasmid

   #226512

   Purpose

   Bacterial cell expression of SNAP-tag2

   Depositor

   Kai Johnsson

   Article

   Kühn et al Nat Chem Biol. 2025 Jul 3. doi: 10.1038/

   Insert

   SNAP-tag2
   

   Tags

   His-tag (10x) and Strep-Tag II,

   Expression

   Bacterial

   Available Since

   Oct. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET-28a(+)-T7-OmpA-MNaseB-6xHis

   Plasmid

   #214809

   Purpose

   Expression of 6xhis-tagged micrococcal nuclease B (MNaseB)

   Depositor

   Peter Sarin

   Article

   Gregorova et al J Biol Chem. 2024 Nov 27;301(1):108020.

   Insert

   OmpA-Micrococcal nuclease B
   

   Tags

   6xhis

   Expression

   Bacterial

   Promoter

   T7 promoter

   Available Since

   Nov. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pOSIP-CT (CamR, P21)

   Plasmid

   #45981

   Depositor

   Keith Shearwin, Drew Endy

   Article

   St-Pierre et al ACS Synth Biol. 2013 Sep 20;2(9):537-41.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   July 26, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. pPROEX Aqua

   Plasmid

   #42889

   Depositor

   Fabienne Merola

   Article

   Erard et al Mol Biosyst. 2013 Feb 2;9(2):258-67. doi

   Insert

   Aquamarine
   

   Tags

   6xHis and TEV cleavage

   Expression

   Bacterial

   Mutation

   T65S and H148G

   Available Since

   March 14, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET28a-SpyCatcher-mi3

   Plasmid

   #112255

   Purpose

   Expresses SpyCatcher-mi3 in bacterial cells as platform for plug-and-display vaccination

   Depositor

   Mark Howarth

   Article

   Bruun et al ACS Nano. 2018 Jul 20. doi: 10.1021/acsn

   Insert

   SpyCatcher-mi3
   

   Tags

   C-tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Sept. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pQE60 R124A wgMDH

    Plasmid

    #204257

    Purpose

    Bacterial expression of R124A wgMDH with IPTG induction - Active site mutant

    Depositor

    Malate dehydrogenase CUREs Community (MCC)

    Article

    Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

    Insert

    MDHG_CITLA
    

    Tags

    6X His

    Expression

    Bacterial

    Mutation

    R124A

    Available Since

    Oct. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBAD_FLAG-GFP(Y39TAG)-6His

    Plasmid

    #223510

    Purpose

    Fluorescent reporter plasmid for genetic code expansion which consists of a modified GFP with a Amber stop codon on position Y39

    Depositor

    Edward Lemke

    Article

    Milles et al J Am Chem Soc. 2012 Mar 21;134(11):5187-

    Insert

    GFP(Y39TAG)
    

    Tags

    6xHis and FLAG

    Expression

    Bacterial

    Mutation

    Y39TAG

    Available Since

    Oct. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHJL1

    Plasmid

    #199376

    Purpose

    Encodes dual cistronic cytosolic GCaMP6s and mScarlet-I optimized for C. elegans expression

    Depositor

    Hang Lu

    Insert

    GCaMP6s::SL2::mScarlet-I
    

    Expression

    Bacterial

    Mutation

    no mutations

    Available Since

    April 24, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. mEGFP-pBAD

    Plasmid

    #54622

    Purpose

    Localization: Protein Expression Vector , Excitation: 488, Emission: 507

    Depositor

    Michael Davidson

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Tags

    6xHis and mEGFP

    Expression

    Bacterial

    Available Since

    July 25, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pET28a-SrtAdelta59

    Plasmid

    #51138

    Purpose

    expression plasmid for N-terminal his tagged S.aureus SrtA delta 59

    Depositor

    Hidde Ploegh

    Article

    Guimaraes et al Nat Protoc. 2013 Sep;8(9):1787-99. doi:

    Insert

    S.aureus SrtA delta 59
    

    Tags

    6x His

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 24, 2014

    Availability

    Academic Institutions and Nonprofits only

15. pBJ7

    Plasmid

    #167136

    Purpose

    A tetracyclin resistant plasmid for Tn7-mediated chromosomal integration of Photorhabdus luminescens luxCDABE operon with Pkan promoter

    Depositor

    Akira Mine

    Article

    Matsumoto et al Plant Commun. 2021 Jul 20;3(1):100227. d

    Insert

    luxCDABE
    

    Use

    Luciferase

    Expression

    Bacterial

    Promoter

    Kanamycin promoter

    Available Since

    June 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET-Cas9-6xHis

    Plasmid

    #62374

    Purpose

    Expression of Cas9-6xHis in bacterial cells

    Depositor

    David Liu

    Article

    Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do

    Insert

    Cas9_6xHis
    

    Tags

    6xHis tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSL-MS2-12X

    Plasmid

    #27119

    Depositor

    Robert Singer

    Article

    Bertrand et al Mol Cell. 1998 Oct . 2(4):437-45.

    Insert

    MS2 binding sites (12)
    

    Expression

    Bacterial

    Available Since

    Dec. 20, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTXB1-CfaC-Gyra

    Plasmid

    #122950

    Purpose

    Bacterial expression of C-intein

    Depositor

    Thomas Muir

    Article

    Stevens et al J Am Chem Soc. 2016 Feb 24;138(7):2162-5

    Insert

    CfaC
    

    Expression

    Bacterial

    Available Since

    April 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pOGG001

    Plasmid

    #113978

    Purpose

    Level 0 module. nptII promoter for constitutive gene expression

    Depositor

    Philip Poole

    Article

    Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

    Insert

    nptII promoter
    

    Expression

    Bacterial

    Mutation

    Domesticated for Golden Gate cloning

    Available Since

    Sept. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. Ube1/PET21d

    Plasmid

    #34965

    Depositor

    Cynthia Wolberger

    Article

    Berndsen et al Anal Biochem. 2011 Nov 1;418(1):102-10.

    Insert

    Ube1 (UBA1 Human)
    

    Tags

    His

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 19, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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