30,535 results

1. pAM236 Ubc4

   Plasmid

   #226361

   Purpose

   Expression of yeast Ubc4

   Depositor

   Andreas Martin

   Article

   Bashore et al Nat Struct Mol Biol. 2015 Sep;22(9):712-

   Insert

   Ubc4 (UBC4 Budding Yeast)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Nov. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. Cut190*_pET21b

   Plasmid

   #215404

   Purpose

   Expression construct for Cut190* gene, codon optimized for expression in E. coli

   Depositor

   Gregg Beckham

   Article

   Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

   Insert

   Cut190*
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   S226P/R228S

   Promoter

   Available Since

   May 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pET28a-VcRibB

   Plasmid

   #187977

   Purpose

   Expresses 3,4-dihydroxy-2-butanone 4-phosphate synthase RibB from Vibrio cholerae

   Depositor

   Audrey Lamb

   Article

   Kenjic et al J Am Chem Soc. 2022 Jul 20;144(28):12769

   Insert

   VcRibB
   

   Use

   Tags

   Hexahistag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   March 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJBL3907

   Plasmid

   #193140

   Purpose

   Clostridium beijerinckii pfl ZTP riboswitch with sfGFP reporter

   Depositor

   Julius Lucks

   Article

   Bushhouse et al Nucleic Acids Res. 2023 Mar 2:gkad110. d

   Insert

   Clostridium beijerinckii (Cbe) pfl ZTP riboswitch with sfGFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J23119

   Available Since

   March 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pJBL6090

   Plasmid

   #193142

   Purpose

   Clostridium beijerinckii pfl ZTP riboswitch A26∆ OFF control with sfGFP reporter

   Depositor

   Julius Lucks

   Article

   Bushhouse et al Nucleic Acids Res. 2023 Mar 2:gkad110. d

   Insert

   Cbe ZTP Riboswitch sfGFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Deleted A26 to disable ligand-binding

   Promoter

   J23119

   Available Since

   March 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. Portal_PhiV1

   Plasmid

   #188811

   Purpose

   Portal protein from phage PhiV1

   Depositor

   Feng Zhang

   Article

   Gao et al Science. 2022 Aug 12;377(6607):eabm4096.

   Insert

   Portal
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Aug. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. Terminase_ST32

   Plasmid

   #188850

   Purpose

   Large terminase from phage ST32

   Depositor

   Feng Zhang

   Article

   Gao et al Science. 2022 Aug 12;377(6607):eabm4096.

   Insert

   Terminase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Aug. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. EpAvs1

   Plasmid

   #188788

   Purpose

   Avs1 from Erwinia piriflorinigrans CFBP5888

   Depositor

   Feng Zhang

   Article

   Gao et al Science. 2022 Aug 12;377(6607):eabm4096.

   Insert

   EpAvs1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   bla

   Available Since

   Aug. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pOPINE GFP nanobody:Halo:His6

   Plasmid

   #111090

   Purpose

   Bacterial expression of a fusion protein consisting of a GFP nanobody (Dr. Brett Collins, Addgene plasmid # 49172), the Halo tag and a His6 tag.

   Depositor

   Lennart Wirthmueller

   Article

   Chen et al Front Plant Sci. 2018 Nov 1;9:1581. doi:

   Insert

   GFP Nanobody
   

   Use

   Tags

   Halo and His6

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   Promoter

   T7 promoter

   Available Since

   June 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCFT4.2

    Plasmid

    #208807

    Purpose

    Expresses the fluorescent protein phiLOV2.1

    Depositor

    François-Xavier Campbell-Valois

    Article

    Tchagang et al Front Microbiol. 2023 Oct 20;14:1245755.

    Insert

    phiLOV2.1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available Since

    Oct. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pET28a SnoopTag-mEGFP-SpyTag

    Plasmid

    #72325

    Purpose

    Bacterial expression of monomeric enhanced green fluorescent protein (mEGFP) containing SnoopTag at the N-terminus and SpyTag at the C-terminus, for programmed polyprotein synthesis.

    Depositor

    Mark Howarth

    Article

    Veggiani et al Proc Natl Acad Sci U S A. 2016 Jan 19. p

    Insert

    pET28a SnoopTag-mEGFP-SpyTag
    

    Use

    Tags

    N-terminal His6 tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Feb. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pFP.R269

    Plasmid

    #138181

    Purpose

    Expresses the split mCherry reporter interrupted by the NrdJ-1 intein (in cis)

    Depositor

    Baojun Wang

    Article

    Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:

    Inserts

    araC (araC )
    mCherryN(NPC).NrdJ-1.(SEI)mCherryC.H6
    

    Use

    Synthetic Biology

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    P(araBAD) and iPC

    Available Since

    May 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. araBAD-BCD20-ClpP-BCD17-ClpX

    Plasmid

    #153301

    Purpose

    Expresses clpX and clpP in E.Coli

    Depositor

    Mikhail Shapiro

    Article

    Lakshmanan et al Nat Chem Biol. 2020 Sep;16(9):988-996. d

    Inserts

    clpX (clpX Escherichia coli)
    clpP (clpP Escherichia coli)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    araBAD

    Available Since

    Sept. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pFEndoNA2

    Plasmid

    #174667

    Purpose

    Expresses GFP-endoNA2 fusion protein for detection of polysialic acid. The endoNA2 is a catalytically inactive form of endosialidase from bacteriophage PK1A2.

    Depositor

    Jukka Finne

    Article

    Jokilammi et al J Immunol Methods. 2004 Dec;295(1-2):149

    Insert

    GFP-endoNA2 fusion protein
    

    Use

    Tags

    Histag

    Expression

    Bacterial

    Mutation

    Promoter

    T5

    Available Since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pTriEx-NTOM20-mVenus-Zdk1

    Plasmid

    #81010

    Purpose

    Expression of Zdk1 anchored to mitochondria

    Depositor

    Klaus Hahn

    Article

    Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

    Insert

    Zdk1
    

    Use

    Tags

    6x His tag, FLAG, NTOM20, and mVenus

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    Promoter

    Available Since

    Aug. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pXW117Ptet2-gfp

    Plasmid

    #160818

    Purpose

    aTc-responsive single-layered circuit

    Depositor

    Baojun Wang

    Article

    Wan et al Nat Commun. 2020 Nov 24;11(1):5961. doi:

    Insert

    PJ23117-30tetR-t-Ptet2-30gfp-t
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PJ23117, Ptet2

    Available Since

    Dec. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pGEX-4T MDM2 WT

    Plasmid

    #16237

    Depositor

    Mien-Chie Hung

    Article

    Zhou et al Nat Cell Biol. 2001 Nov . 3(11):973-82.

    Insert

    MDM2 (MDM2 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Nov. 30, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCre

    Plasmid

    #236212

    Purpose

    Arabinose-inducible Cre recombinase

    Depositor

    Matthew Waldor

    Article

    Basta et al Nat Microbiol. 2025 Mar 27. doi: 10.1038

    Insert

    Cre recombinase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    April 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. HC-M

    Plasmid

    #173481

    Purpose

    An rrnB P1-based GFP ATP reporter in E. coli

    Depositor

    Rahul Sarpeshkar

    Article

    Deng et al BMC Biol. 2021 May 17;19(1):101. doi: 10

    Insert

    GFP-mut2
    

    Use

    Synthetic Biology

    Tags

    ssrA degradation tag

    Expression

    Bacterial

    Mutation

    Promoter

    rrnB P1

    Available Since

    Aug. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET-Bxb1-His

    Plasmid

    #123132

    Purpose

    Bacterial expression plasmid for wild-type Bxb1 with a C-terminal His-tag.

    Depositor

    David Liu

    Article

    Bessen et al Nat Commun. 2019 Apr 26;10(1):1937. doi:

    Insert

    Bxb1 integrase
    

    Use

    Tags

    6x His tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    April 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge