30,738 results

1. pDEST17-UbE2E2

   Plasmid

   #15788

   Depositor

   Wade Harper

   Article

   Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

   Insert

   Ubiquitin conjugating enzyme UbE2E2 (UBE2E2 Human)
   

   Tags

   His

   Expression

   Bacterial

   Available Since

   Sept. 28, 2007

   Availability

   Academic Institutions and Nonprofits only

2. pet42c(+)-GST-USP5

   Plasmid

   #61750

   Purpose

   express N-terminally GST tagged human USP5

   Depositor

   Yihong Ye

   Article

   Liu et al Elife. 2014;3:e01369. doi: 10.7554/eLife

   Insert

   USP5 (USP5 Human)
   

   Tags

   GST

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Feb. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

3. pCOLA-Gent-EM7-Erv1p-PDI

   Plasmid

   #202486

   Purpose

   Expresses sulfhydryl oxidase (Erv1p) and protein disulfide bond isomerase (PDI) constitutively

   Depositor

   Michael Lynch

   Article

   Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

   Insert

   sulfhydryl oxidase (Erv1p) and protein disulfide bond isomerase (PDI) (ERV1 Human, Budding Yeast)
   

   Expression

   Bacterial

   Promoter

   EM7

   Available Since

   Nov. 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBAD-LSSmApple

   Plasmid

   #191259

   Purpose

   Bacterial expression plasmids for Long Stokes shift red fluorescent protein LSSmApple

   Depositor

   Robert Campbell

   Article

   Ejike et al Biochemistry. 2024 Jan 2;63(1):171-180.

   Insert

   LSSmApple
   

   Tags

   His tag

   Expression

   Bacterial

   Available Since

   Oct. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET28b-ULP1-405-621

   Plasmid

   #229224

   Purpose

   For expression of ULP1 protein

   Depositor

   Andy LiWang

   Insert

   ULP1 (ULP1 Budding Yeast)
   

   Expression

   Bacterial

   Mutation

   405-621 (truncated)

   Available Since

   Jan. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pES001 MBP-GSK3β-HA-His

   Plasmid

   #154010

   Purpose

   Expresses MBP-GSK3β-HA-His (human Glycogen Synthase Kinase 3β as a fusion protein with MBP, HA, and His- tags) in E.coli

   Depositor

   Jesse Zalatan

   Article

   Gavagan et al Cell Syst. 2020 Jun 24;10(6):515-525.e5.

   Insert

   GSK3B (GSK3B Human)
   

   Tags

   HA tag, His6, and MBP

   Expression

   Bacterial

   Promoter

   Tac

   Available Since

   Jan. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSCrhaB2

   Plasmid

   #113634

   Purpose

   An expression vector containing a rhamnose-inducible promoter to regulate gene expression in Burkholderia cenocepacia

   Depositor

   Miguel Valvano

   Article

   Cardona et al Plasmid. 2005 Nov;54(3):219-28. doi: 10.

   Type

   Empty backbone

   Expression

   Bacterial

   Promoter

   PrhaB

   Available Since

   Aug. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBJ2

   Plasmid

   #167131

   Purpose

   A gentamycin resistant plasmid for Tn7-mediated chromosomal integration of Photorhabdus luminescens luxCDABE operon with Pkan promoter

   Depositor

   Akira Mine

   Article

   Matsumoto et al Plant Commun. 2021 Jul 20;3(1):100227. d

   Insert

   luxCDABE
   

   Use

   Luciferase

   Expression

   Bacterial

   Promoter

   Kanamycin promoter

   Available Since

   June 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGEX6P1-EGFP-anti-mouse-IgG(kappa)-nanobody

   Plasmid

   #138132

   Purpose

   Expresses GST-tagged EGFP-anti-mouse-IgG(kappa)-nanobody in E. coli cells.

   Depositor

   Kazuhisa Nakayama

   Article

   Katoh et al Mol Biol Cell. 2020 Jul 29:mbcE20040250.

   Insert

   EGFP-anti-mouse-IgG(kappa)-nanobody
   

   Tags

   GST

   Expression

   Bacterial

   Available Since

   July 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. RhoG

    Plasmid

    #55694

    Purpose

    expression of mouse RhoG

    Depositor

    Julian Downward

    Article

    Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

    Insert

    RhoG (Rhog Mouse)
    

    Expression

    Bacterial

    Promoter

    lac

    Available Since

    Sept. 30, 2014

    Availability

    Academic Institutions and Nonprofits only

11. pDEST17-UbE2R2

    Plasmid

    #15806

    Depositor

    Wade Harper

    Article

    Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

    Insert

    Ubiquitin conjugating enzyme UbE2R2 (UBE2R2 Human)
    

    Tags

    His

    Expression

    Bacterial

    Available Since

    Sept. 28, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRSFDUET-1_VNp-anti-GFP mAb

    Plasmid

    #214955

    Purpose

    Bacterial expression of anti-GFP mAb light and heavy chain VNp fusions.

    Depositor

    Dan Mulvihill

    Article

    Baker et al Open Biol. 2025 Feb;15(2):240229. doi: 1

    Inserts

    VNp-anti-GFP mAb heavy chain
    VNp-anti-GFP mAb light chain
    

    Tags

    VNp6

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHH0103_NXF1_TAP-C

    Plasmid

    #110038

    Purpose

    Protein expression and purification of NXF1_TAP-C

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

    Insert

    NXF1_TAP-C (NXF1 Human)
    

    Expression

    Bacterial

    Available Since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pOPINF

    Plasmid

    #26042

    Depositor

    Ray Owens

    Article

    Berrow et al Nucleic Acids Res. 2007 . 35(6):e45.

    Insert

    His3C-lacZ
    

    Tags

    MAHHHHHHSSGLEVL

    Expression

    Bacterial, Insect, and Mamm…

    Available Since

    May 17, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCDF-Frb-v1

    Plasmid

    #201923

    Purpose

    E. coli vector expressing FrbA, FrbB, FrbC, FrbD and FrbE for the biosynthesis of non-hydrolyzable phosphoserine from phosphoenolpyruvate.

    Depositor

    Richard Cooley, Ryan Mehl

    Article

    Zhu et al ACS Cent Sci. 2023 Apr 10;9(4):816-835.

    Inserts

    FrbA
    FrbB
    FrbC
    FrbD
    FrbE
    

    Expression

    Bacterial

    Promoter

    T7 (modified), T7 (modified, moderate), T7 (modif…

    Available Since

    June 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. JMJD1BA-c068

    Plasmid

    #53627

    Purpose

    bacterial expression plasmid

    Depositor

    Nicola Burgess-Brown

    Insert

    JMJD1BA
    

    Tags

    TEV-His6-Flag

    Expression

    Bacterial

    Mutation

    Bacterial expression for structure determination;…

    Promoter

    T7

    Available Since

    June 17, 2014

    Availability

    Academic Institutions and Nonprofits only

17. pGEX-p47PX(aa2-132)

    Plasmid

    #19007

    Depositor

    Michael Yaffe

    Article

    Kanai et al Nat Cell Biol. 2001 Jul . 3(7):675-8.

    Insert

    p47phox (NCF1 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    aa2-132 (PX domains)

    Available Since

    Aug. 25, 2008

    Availability

    Academic Institutions and Nonprofits only

18. pNatB (pACYCduet-naa20-naa25)

    Plasmid

    #53613

    Purpose

    Allows expression of the fission yeast NatB complex - chloramphenicol marker

    Depositor

    Dan Mulvihill

    Article

    Johnson et al PLoS One. 2010 Dec 23;5(12):e15801. doi:

    Inserts

    naa20 (naa20 Fission Yeast)
    naa25 (naa25 Fission Yeast)
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    June 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pJ211_rha_WT_low

    Plasmid

    #128851

    Purpose

    Low copy number plasmid for expression of E. coli flagellin from a rhamnose-inducible promoter

    Depositor

    Mark Pallen

    Article

    Thomson et al Curr Res Biotechnol. 2020 Nov;2:45-52. d

    Insert

    Flagellin
    

    Expression

    Bacterial

    Available Since

    Feb. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pJMP3653

    Plasmid

    #222348

    Purpose

    Expression vector with PabstBR promoter, kanR marker, and replicates in A. baumannii and E. coli; empty vector plasmid with MCS for cloning - we recommend NcoI/BamHI.

    Depositor

    Jason Peters

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    July 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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