33,902 results
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Plasmid#74495PurposeNicking Cas9 derived from pWT Cas9 (Addgene #44250). Ampicillin resistance marker, the tetracycline repressor (TetR), a ColE1 origin of replication and Streptococcus pyogenes Cas9 D10ADepositorInsertCas9 D10A
UseSynthetic BiologyExpressionBacterialMutationD10APromoterpTetoAvailable sinceMay 16, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSG4K5
Plasmid#74492PurposeFor cloning and constitutive expression of sgRNAs in E.coli. New sgRNAs are cloned into SapI sites and multiplex sgRNAs are cloned into BsaI sites. Kanamycin resistant with pSC101 origin of rep.DepositorInsertsgRNA
UseCRISPR and Synthetic BiologyExpressionBacterialPromoterJ23119Available sinceMay 16, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pEvol-pAcFRS.1.t1
Plasmid#73545PurposetRNA synthetase/tRNA pair for the in vivo incorporation of several non-standard amino acids, into proteins in E coli in response to the amber codon, TAGDepositorInsertspAcFRS.1.t1
pAcFRS.1.t1
ExpressionBacterialAvailable sinceMarch 25, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGex-4T-2_GABARAP
Plasmid#73948PurposeFor recombinant expression of human GABARAP in E.coliDepositorAvailable sinceMarch 25, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pKB01_mCherry
Plasmid#69104PurposeExpresses mCherry (codon adapted for Bacillus subtilis); under control of Zinc inducible promoter PZn. Plasmid integrates in S. pneumoniae bgaA locus by double cross-over.DepositorInsertmCherry
UseSynthetic BiologyExpressionBacterialAvailable sinceOct. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAWP78
Plasmid#61263PurposeSmall IncP-based broad host range replicating vector.DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialAvailable sinceFeb. 26, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pET11a-SUMO1
Plasmid#53138PurposeE.coli expression of untagged mature human SUMO1 (amino acids 1-97)DepositorPublicationAvailable sinceSept. 17, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMV306DIhsp+LuxG13
Plasmid#49999PurposeIntegrase removed to increase stability of the plasmid in mycobacteriaDepositorInsertBacterial luciferase operon + G13 promoter
ExpressionBacterialMutationIt contains a gram-positive enhanced translation …PromoterG13 from M. marinum G13 promoter driving luxCAvailable sinceMay 21, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
PCRT7 tetR pLtetO MBP-MEK1 XX Amp
Plasmid#53225Purposehuman MEK1 with MBP and HIS tags, with Ser218 and Ser222 changed to amber codons, for use in phosphoprotein synthesisDepositorInsertMEK1 (MAP2K1 Human)
UsePhosphoprotein synthesisTagsHIS and MBPExpressionBacterialMutationCodon optimized for E.coli, both codons for Ser21…PromoterpLtetOAvailable sinceMay 7, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pOSIP-CH (CamR, HK022)
Plasmid#45980DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialAvailable sinceJuly 26, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Pet28a_Synthetic_Human_H2AZ.1
Plasmid#42629DepositorInsertHuman H2AZ.1 (H2AZ1 Human, Synthetic, Codon Optimized)
TagsUntaggedExpressionBacterialMutationCodon OptimizedPromoterT7Available sinceMarch 21, 2013AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
BBa_J72214-BBa_J72090
Plasmid#40782DepositorInsertVioABCDE
UseSynthetic Biology; PhagemidExpressionBacterialAvailable sinceOct. 31, 2012AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pQE-hTR
Plasmid#21811DepositorAvailable sinceSept. 13, 2012AvailabilityAcademic Institutions and Nonprofits onlyCited by -
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pBAD/HisB-Fast-FT
Plasmid#31916DepositorInsertFast-FT
TagsHisExpressionBacterialMutationM18V/E34K/K69R/L84W/S149T/A224S compared to mCher…PromoteraraBAvailable sinceSept. 7, 2011AvailabilityAcademic Institutions and Nonprofits only -
pET empty polycistronic destination vector (2D)
Plasmid#29774DepositorTypeEmpty backboneExpressionBacterialAvailable sinceJune 9, 2011AvailabilityAcademic Institutions and Nonprofits only -
pET15b-hnRNP-H
Plasmid#23020DepositorAvailable sinceJune 4, 2010AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pET28CLY9
Plasmid#21769DepositorInsertECFP -EYFP linked by flexible linker containing 9 SGGSGG repeats
TagsHisExpressionBacterialAvailable sinceDec. 1, 2009AvailabilityAcademic Institutions and Nonprofits onlyCited by -
RCAS(B)-Rheb-Q64L
Plasmid#21050DepositorInsertRas-homolog enriched in brain (RHEB Human)
UseRetroviralTagsFLAGExpressionBacterialMutationglutamine 64 to leucineAvailable sinceAug. 28, 2009AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pTrcHis-p47
Plasmid#21268DepositorAvailable sinceJuly 1, 2009AvailabilityAcademic Institutions and Nonprofits onlyCited by