31,059 results
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Plasmid#215303PurposeExpresses sfGFP under rhamnose induction (RhaS + RhaR + pRhaB-sfGFP) with a BBR1 backbone.DepositorInsertsfGFP
UseSynthetic BiologyTagsNoneExpressionBacterialPromoterPRhaBAvailable SinceJuly 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pWM_12x601_30bpLinker
Plasmid#157789PurposeEcoRV digestion produces 12x601 chromatin assembly construct with 30 bp linker lengths in addition to carrier DNA that prevents overassembly of nucleosomesDepositorInsert12x601_30bp linker
ExpressionBacterialAvailable SinceMarch 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
OmpA3-PNGaseF-TEV-His6
Plasmid#114274PurposeAmidase removing almost all N-linked oligosaccharides from glycoproteins. This PNGase F construct can have its hexa-his removed.DepositorInsertPeptide: N-glycosidase F
TagsOmpA3 leader sequence (for periplasmic localisati…ExpressionBacterialPromoterT7Available SinceAug. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCm C-term MBP NikJC199U
Plasmid#174365PurposeExpression plasmid with a TEV removable C-term 8xHis tagged MBP tag, and Chloramphenicol resistance. NikJ C199U cloned in the cloning site.DepositorInsertnikJ, nikkomycin biosynthesis protein P1 [Streptomyces tendae]
Tags8xHis, MBP, and TEVExpressionBacterialMutationC199UPromoterT7Available SinceDec. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMevT
Plasmid#17815DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialAvailable SinceMay 16, 2008AvailabilityAcademic Institutions and Nonprofits only -
pSW002-Pc-mRuby2
Plasmid#111250PurposeBroad host-range bacterial expression vector with constitutive Pc promoter; for tagging bacteria with mRuby2 (codon optimized for P. fluorescens)DepositorInsertmRuby2
ExpressionBacterialMutationmRuby2 is codon optimized for expression in P. fl…PromoterPcAvailable SinceAug. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJ1996_v2
Plasmid#140664PurposedCas9 & Csy4DepositorInsertdCas9 & Csy4
UseSynthetic BiologyExpressionBacterialAvailable SinceJuly 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSL-MS2-6X
Plasmid#27118DepositorInsertMS2 binding sites (6)
ExpressionBacterialAvailable SinceDec. 20, 2010AvailabilityAcademic Institutions and Nonprofits only -
pET His6 GFP TEV LIC cloning vector (1GFP)
Plasmid#29663DepositorHas ServiceCloning Grade DNATypeEmpty backboneTags6xHis, GFP, and TEV cleavage siteExpressionBacterialPromoterT7-lacO (lactose/IPTG inducible)Available SinceJune 7, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCAG-BirA*-dCas9-eGFP
Plasmid#84779PurposeExpresses BirA*-dCas9-eGFP in mammalian cells.DepositorInsertBirA*-dCas9-eGFP
UseCRISPRTagscMycExpressionBacterial and MammalianPromoterCAGAvailable SinceJuly 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
His6-EGFP-PH-PLCD1
Plasmid#183675PurposePtdIns(4,5)P2 biosensor for purification from bacteriaDepositorInsertPLCD1 (PLCD1 Human)
TagsHis6-EGFPExpressionBacterialMutationAmino acids 11-140PromoterT7Available SinceMay 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST14-SpyCatcher
Plasmid#35044DepositorInsertSpyCatcher
TagsHis6ExpressionBacterialMutationDeletion of C-terminal till I113, and point mutat…Available SinceApril 18, 2012AvailabilityAcademic Institutions and Nonprofits only -
pET21b Hot-PETase
Plasmid#225761PurposeExpresses codon-optimized Hot-PETase in E. coli with a C-terminal his-tagDepositorInsertHot-PETase
Tags6xHis TagExpressionBacterialPromoterT7Available SinceJan. 22, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBAD-pHlorina
Plasmid#182763PurposepH-sensitive RFP pHlorina for detection of efferocytosisDepositorInsertpHlorina
ExpressionBacterialAvailable SinceSept. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
mu-FLT3Lv1-pTRIP
Plasmid#163519PurposeUsed to construct lentivirus to express murine FLT3 ligand (variant-1) in mammalian cellsDepositorAvailable SinceJan. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
A24M04 mIgM heavy chain-pFEEKW
Plasmid#234809PurposeExpress membrane form of IgM heavy chain containing the VH region of the monoclonal antibody, A24M04, specific for human papilloma virus16 L1DepositorInsertA24M04 membrane IgM heavy chain (IGHM Human)
UseLentiviralExpressionBacterial and MammalianPromoterEEK promoterAvailable SinceMarch 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET-HRas-1-167-WT
Plasmid#245306PurposeExpresses G-domain of human HRas (residues 1-167 wild type) in E. coliDepositorInsertHRas (HRAS Human)
Tags6HisExpressionBacterialMutationdeleted amino acids 168-189PromoterT7lacAvailable SinceOct. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
EF1α-Lox2272-LoxP-MARKS·mTurquoise2-NLS·turboRFP-Lox2272-LoxP
Plasmid#198753PurposeCre reporter, which switches from membraneous mTurquoise to nuclear RFP upon Cre recombinase activityDepositorInsertEF1a promotor-Lox2272-LoxP-MARKS-mTurquoise2-NLS.turboRFP-Lox2272-LOXP
UseLentiviralExpressionBacterial and MammalianMutationSee depositor comments belowPromoterEF1aAvailable SinceJuly 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET24a isPETase
Plasmid#225759PurposeExpresses codon-optimized PETase from Ideonella sakaiensis in E. coli without native signal sequence and with a C-terminal his-tagDepositorInsertisPETase
Tags6xHis TagExpressionBacterialPromoterT7Available SinceDec. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
PT7lacO-EF-Tu-His8
Plasmid#224370PurposeExpress EF-Tu protein for his-tagged purificationDepositorAvailable SinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only