30,738 results

1. pET151-HIS-TEV-GST-PP-RPN13

   Plasmid

   #73741

   Purpose

   Expression of hRPN13 in E.coli

   Depositor

   Chris Hill

   Article

   VanderLinden et al J Biol Chem. 2017 Apr 25. pii: jbc.M117.

   Insert

   RPN13 (ADRM1 Human)
   

   Tags

   6xHIS and GST

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   April 27, 2017

   Availability

   Academic Institutions and Nonprofits only

2. pBAD18HMGR

   Plasmid

   #19041

   Depositor

   Jay Keasling

   Article

   Pitera et al Metab Eng. 2007 Mar . 9(2):193-207.

   Insert

   truncated 3-hydroxy-3-methyl-glutaryl-coenzyme A Reductase (HMG1 Budding Yeast)
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   Sept. 8, 2008

   Availability

   Academic Institutions and Nonprofits only

3. pSG 25

   Plasmid

   #63867

   Purpose

   Expression of beta-galactosidase at high levels in E. coli

   Depositor

   Albert Dahlberg

   Article

   O'Connor et al Proc Natl Acad Sci U S A. 1993 Oct 1;90(

   Insert

   lacZ (lacZ Escherichia coli)
   

   Expression

   Bacterial

   Promoter

   PtacI

   Available Since

   April 27, 2015

   Availability

   Academic Institutions and Nonprofits only

4. 2Bc-T MBP_BoMoC(ed)_6xH

   Plasmid

   #186461

   Purpose

   Recombinant B. mori truncated R2 RT expression

   Depositor

   Kathleen Collins

   Article

   Upton et al Proc Natl Acad Sci U S A. 2021 Oct 19;11

   Insert

   B. mori truncated R2 RT
   

   Tags

   6xHis and MBP

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFA6a-5FLAG-KanMX6

   Plasmid

   #15983

   Depositor

   Eishi Noguchi

   Article

   Noguchi et al Biotechnol J. 2008 Oct;3(9-10):1280-5. d

   Type

   Empty backbone

   Tags

   5xFlag

   Expression

   Bacterial

   Available Since

   Oct. 31, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMyNT

   Plasmid

   #42191

   Depositor

   Matthias Wilmanns, Annabel Parret

   Article

   Beckham et al Protein Sci. 2020 Oct 2. doi: 10.1002/pr

   Type

   Empty backbone

   Tags

   His6

   Expression

   Bacterial

   Promoter

   ColE1

   Available Since

   Aug. 5, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEF1-Pls4-sfGFP

   Plasmid

   #205751

   Purpose

   Expresses sfGFP under IPTG inducible promoter Pls4 in high copy pTRKH2 gram-positive shuttle vector. Used in Lactobacillus gasseri. High strength.

   Depositor

   Jerome Bonnet

   Article

   Fristot et al ACS Synth Biol. 2024 Mar 15;13(3):951-95

   Type

   Empty backbone

   Use

   Shuttle vector gram+ gram-

   Expression

   Bacterial

   Promoter

   Pls4 (Phyperspank mutant, IPTG inducible)

   Available Since

   July 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pKEK3230

   Plasmid

   #234102

   Purpose

   CCP expression in diverse gram-negative bacteria, with pre-cloned Golden Gate Compatible (BsaI) restriction sites to facilitate promoter swapping.

   Depositor

   Karl Klose

   Article

   Armstrong et al Appl Environ Microbiol. 2025 May 12:e000

   Insert

   FuGFP
   

   Expression

   Bacterial

   Promoter

   pJ23100-BsaI

   Available Since

   May 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBad33_IS605_reRNA

   Plasmid

   #205321

   Purpose

   Bacterial expression of IS605 TnpB and its reRNA

   Depositor

   Haoyi Wang

   Article

   Xiang et al Nat Biotechnol. 2023 Jun 29. doi: 10.103

   Insert

   IS605 TnpB
   

   Expression

   Bacterial

   Available Since

   Oct. 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET28a-XPO5-His6

    Plasmid

    #171562

    Purpose

    Encodes human XPO5 protein

    Depositor

    Yan Zeng

    Insert

    XPO5 (XPO5 Human)
    

    Expression

    Bacterial

    Available Since

    July 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBbS8a-RFP

    Plasmid

    #35274

    Depositor

    Jay Keasling

    Article

    Lee et al J Biol Eng. 2011 Sep 20;5:12.

    Insert

    RFP
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    BAD

    Available Since

    Feb. 16, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCDB179-HotPETase

    Plasmid

    #218701

    Purpose

    Encodes codon optimized HotPETase enzyme for bacterial expression

    Depositor

    Gregg Beckham

    Article

    Norton-Baker et al Sci Rep. 2024 Jun 24;14(1):14449. doi: 1

    Insert

    HotPETase
    

    Tags

    10xHis

    Expression

    Bacterial

    Available Since

    May 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET28a hMDH2

    Plasmid

    #204230

    Purpose

    Bacterial expression of mitochondrial human malate dehydrogenase 2 (hMDH2) transcript variant 1; Use in CURES (integration of research into undergraduate teaching labs).

    Depositor

    Malate dehydrogenase CUREs Community (MCC)

    Insert

    hMDH2 (MDH2 Human)
    

    Tags

    6X His

    Expression

    Bacterial

    Mutation

    Does not include transit peptide and includes a M…

    Promoter

    Lac

    Available Since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET14b – E. coli Exonuclease I

    Plasmid

    #104552

    Purpose

    bacterial expression of Exonuclease I

    Depositor

    Charles Bell

    Article

    Korada et al Nucleic Acids Res. 2013 Jun;41(11):5887-

    Insert

    Exonuclease I (sbcB E.coli)
    

    Tags

    6X-His tag followed by thrombin site

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Dec. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. LM670

    Plasmid

    #191552

    Purpose

    LM670_MSG-AGGA-promoter-ccdb-TTCC-GShis

    Depositor

    David Baker

    Article

    Wicky et al Science. 2022 Oct 7;378(6615):56-61. doi

    Insert

    ccdb
    

    Tags

    HIS-tag

    Expression

    Bacterial

    Available Since

    Feb. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. UbcH5A

    Plasmid

    #61081

    Purpose

    express a human E2

    Depositor

    Cynthia Wolberger

    Article

    Wiener et al Nat Struct Mol Biol. 2013 Sep;20(9):1033

    Insert

    UbcH5A (UBE2D1 Human)
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 29, 2015

    Availability

    Academic Institutions and Nonprofits only

17. pET28 MBP NAAEF-AMPKa1 (13-476,529-550)-b2(76-272)-g1(24-327)

    Plasmid

    #177850

    Purpose

    Bacterial Expression of AMPK

    Depositor

    Karsten Melcher

    Article

    Yan et al Science. 2021 Jul 23;373(6553):413-419.

    Insert

    AMPK (a1b2g1)
    

    Tags

    MBP

    Expression

    Bacterial

    Available Since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pRSFDuet-1 ElonginB, ElonginC

    Plasmid

    #230999

    Purpose

    Dual expression vector containing mouse ElonginB and ElonginC for E.coli protein purification

    Depositor

    Michael Rape

    Article

    Manford et al Cell. 2020 Oct 1;183(1):46-61.e21. doi:

    Inserts

    Elongin B (Elob Mouse)
    Elongin C (Eloc Mouse)
    

    Expression

    Bacterial

    Available Since

    Jan. 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBbS8k-RFP

    Plasmid

    #35276

    Depositor

    Jay Keasling

    Article

    Lee et al J Biol Eng. 2011 Sep 20;5:12.

    Insert

    RFP
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    BAD

    Available Since

    Feb. 16, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBbA5c-MevT(CO)-MBIS(CO, ispA)

    Plasmid

    #35151

    Depositor

    Jay Keasling, Taek Soon Lee

    Article

    Peralta-Yahya et al Nat Commun. 2011 Sep 27;2:483. doi: 10.1

    Insert

    AtoB(co)-HMGS(co)-HMGR(co)-MK(co)-PMK(co)-PMD-Idi-IspA
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Mutation

    R364S in Mevalonate Kinase

    Promoter

    LacUV5,

    Available Since

    July 23, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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