29,691 results
-
Plasmid#180451PurposeE. coli expression of soluble MHC proteinDepositorInsertHLA-A*03:01
UseTagsBSP41ExpressionBacterialMutationReplacement of transmembrane domain with enzymati…PromoterAvailable sinceMarch 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEND-227_Cacnes_mCerulean
Plasmid#225614PurposeReplicative vector for blue fluorescent reporter protein mCerulean strong constitutive recombinant expression in C. acnes. pBRESP36A with P(BBa_J23119)+RBS_1+mCerulean.DepositorInsertmCerulean
UseSynthetic BiologyTags6xHisExpressionBacterialMutationCodon optimized for Cutibacterium acnesPromoterAvailable sinceFeb. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
UbC-blasticidine-P2A-TETon-3G transactivator
Plasmid#200191PurposeA TETon activator, which combined with the DiLiCre 2.0 plasmid, drives the expression of DiLiCre 2.0 upon doxycycline. DiLiCre 2.0 is a cre recombinase that is activated by violet light.DepositorInsertblasticidine - P2A - TETon-3G transactivator
UseLentiviralTagsExpressionBacterial and MammalianMutationPromoterUbCAvailable sinceAug. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTKDP-dhfr
Plasmid#71323PurposeDonor plasmid - trimethoprim resistant donor cassetteDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJan. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
-
-
-
pET-28a-His6-SUMO-Munc18-1wt
Plasmid#135550PurposeExpress rat Munc18-1 in E. coli BL21, purified via HisPur Ni-NTA ResinDepositorInsertMunc18-1wt
UseTags6xHis tag, thrombin recognition and cleavage site…ExpressionBacterialMutationNo ATG start codonPromoterT7 promoterAvailable sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pChillyBoys_gen1
Plasmid#228495PurposepACYCDuet-1 encoding Oleispira antarctica GroEL and GorES low temp chaperonins for enhanced low-temperature induction (E. coli based protein expression)DepositorInsertsgroEL
groES
UseTagsExpressionBacterialMutationPromoterT7 PromoterAvailable sinceJan. 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pTrcHUM
Plasmid#19003DepositorInsertgamma-humulene synthase
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceSept. 8, 2008AvailabilityAcademic Institutions and Nonprofits only -
pET-29b(+)-mSandy2
Plasmid#177760PurposeExpresses mSandy2 using the T7 PromoterDepositorInsertmSandy2
UseTags6xHis tagExpressionBacterialMutationPromoterT7 PromoterAvailable sinceJan. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
Ki67∆Nterm-mNeonGreen-IRESpuro2
Plasmid#183740PurposeExpresses Ki67∆Nterm-mNeonGreenDepositorInsertMKI67 LR domain + all Ki-67 repeats (MKI67 Human)
UseTagsmNeonGreenExpressionBacterial and MammalianMutationPromoterCMVAvailable sinceJuly 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGEX4T3-GST-Tev
Plasmid#177142PurposeBacterial vector for expression of an N-terminal GST fusion of the Tev proteaseDepositorInsertTev
UseTagsGSTExpressionBacterialMutationPromoterTacAvailable sinceDec. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET30a-His6-LOXCAT
Plasmid#140084PurposeFusion of A. viridans lactate oxidase (LOX) and E. coli catalase (CAT) that converts lactate into pyruvate without producing H2O2.DepositorInsertA fusion of lactate oxidase from A. viridans and catalase from E. coli
UseTagsHis6ExpressionBacterialMutationPromoterT7Available sinceSept. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCas9cur
Plasmid#71538PurposeConstitutive expression of Cas9 gene and inducible expression of the plasmid curing system for CRISPR mediated genome editing of E. coli.DepositorInsertsCas9
Plasmid curing system
UseCRISPRTagsExpressionBacterialMutationPromoterpBADAvailable sinceFeb. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGEM-NLS-Cre-2A-mCherry-FKF
Plasmid#119868PurposeDonor cassette containing NLS-tagged Cre, a Thosea asigna virus peptide (2A), mCherry and SV40 polyA tail, followed by FRT site-flanked Kanamycin selection geneDepositorInsertNLS-Cre, 2A, mCherry and SV40 polyA, followed by FRT site-flanked Kanamycin selection gene
UseTagsmCherryExpressionBacterialMutationPromoterAvailable sinceApril 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pY3
Plasmid#50606PurposeOrigin Of Replication: p15a, Selection Markers: Amp, Promoters: PLacUV5DepositorInsertstyrB
tyrA
aroC
aroA
aroL
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterlacUV5Available sinceNov. 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
pACYCDuet-Myc-MBP-HA
Plasmid#222711PurposeExpression Myc-tagged F-box protein and MBP-HA-tagged substrate in bacterial cellsDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJ2076.2_TS
Plasmid#140667PurposeToggle switch (TS)DepositorInsertToggle switch (TS)
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJuly 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
BBa_J72214-BBa_J72090
Plasmid#40782DepositorInsertVioABCDE
UseSynthetic Biology; PhagemidTagsExpressionBacterialMutationPromoterAvailable sinceOct. 31, 2012AvailabilityAcademic Institutions and Nonprofits only