30,260 results

1. pNP1830

   Plasmid

   #222728

   Purpose

   pNP561 - T5 Target with T7 Inducible IS621 Recombinase

   Depositor

   Patrick Hsu

   Article

   Durrant et al Nature. 2024 Jun;630(8018):984-993. doi:

   Insert

   IS621 Recombinase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJL1

   Plasmid

   #69496

   Purpose

   pJL1 expression vector with sfGFP

   Depositor

   Michael Jewett

   Article

   Martin et al Nat Commun. 2018 Mar 23;9(1):1203. doi:

   Insert

   Super folder GFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 1, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pET30a-AlkB-D135S

   Plasmid

   #79051

   Purpose

   Express mutant AlkB protein (D135S) in E. coli BL21(DE3)

   Depositor

   Tao Pan

   Article

   Zheng et al Nat Methods. 2015 Sep;12(9):835-7. doi:

   Insert

   AlkB (alkB E.coli)
   

   Use

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   A truncated AlkB with deletion of the amino (N)-t…

   Promoter

   T7

   Available since

   Aug. 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBAD-BirA-eCPX-AP

   Plasmid

   #121907

   Purpose

   Expresses BirA and eCPX-AP

   Depositor

   Per Svenningsen

   Article

   Granhoj et al Sci Rep. 2019 Mar 11;9(1):4118. doi: 10.

   Inserts

   BirA (birA Synthetic)
   Enhanced Circularly Permuted Outer Membrane Protein X
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7 and pBAD promoter

   Available since

   April 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. GST-TEV-FUS

   Plasmid

   #29629

   Depositor

   Aaron Gitler

   Article

   Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

   Insert

   FUS (FUS Human)
   

   Use

   Tags

   GST and TEV

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 18, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET-28a-hPKM1

   Plasmid

   #44241

   Depositor

   Matthew Vander Heiden, Lewis Cantley

   Article

   Christofk et al Nature. 2008 Mar 13;452(7184):181-6. doi

   Insert

   Pyruvate Kinase M1 (PKM Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTP955

   Plasmid

   #104164

   Purpose

   Bacterial expression plasmid of anti-rabbit IgG Fc nanobody TP897 (3x Cysteines)

   Depositor

   Dirk Görlich

   Article

   Pleiner et al J Cell Biol. 2018 Mar 5;217(3):1143-1154

   Insert

   Anti-rabbit IgG Fc specific nanobody TP897 (3xCysteines)
   

   Use

   Tags

   14xHistidine tag and NEDD8 from Brachypodium dist…

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET tdTomato LIC cloning vector (H6-tdTomato)

   Plasmid

   #86928

   Purpose

   pET LIC cloning vector for Biotin-His6-tev-yORF-tdTomato

   Depositor

   Chris Jeans

   Type

   Empty backbone

   Use

   Tags

   6xHis, AviTag (biotin), TEV cleavage site, and td…

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pQFT

   Plasmid

   #203674

   Purpose

   pQF derivative with promoter PQ5 exchanged for promoter PQJ

   Depositor

   Urs Jenal

   Article

   Klotz et al Appl Environ Microbiol. 2023 May 18:e002

   Type

   Empty backbone

   Use

   Tags

   3xFLAG

   Expression

   Bacterial

   Mutation

   Promoter

   PQJ

   Available since

   Dec. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGRB

    Plasmid

    #71539

    Purpose

    for expression of gRNA in E. coli

    Depositor

    Tao Chen

    Article

    Li et al Metab Eng. 2015 Sep;31:13-21. doi: 10.10

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J231196

    Available since

    Feb. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pC018 - LshCas13a from Leptotrichia shahii

    Plasmid

    #91908

    Purpose

    Expresses LshCas13a for bacterial expression and contains backbone for spacer cloning

    Depositor

    Feng Zhang

    Article

    Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

    Insert

    LshCas13a and guide expression cassette
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. GB1-A11(2-196, D40G)-Strep

    Plasmid

    #196210

    Purpose

    Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-A11(2-196, D40G)-Strep.

    Depositor

    Lalit Deshmukh

    Article

    Shihora et al ACS Chem Neurosci. 2023 Jul 11. doi: 10.

    Insert

    Annexin A11 (ANXA11 Human)
    

    Use

    Tags

    GB1-6xHis-TEV and Strep-II tag

    Expression

    Bacterial

    Mutation

    D40G

    Promoter

    Available since

    July 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pdCas9

    Plasmid

    #46569

    Purpose

    Expresses the tracrRNA, the dCas9 catalytic site mutant and a CRISPR array designed for the easy cloning of new spacers.

    Depositor

    Luciano Marraffini

    Article

    Bikard et al Nucleic Acids Res. 2013 Jun 12.

    Inserts

    tracrRNA
    dcas9
    CRISPR array
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    D10A, H840A

    Promoter

    Available since

    Aug. 27, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET-28c(+)-YTHDF2

    Plasmid

    #102275

    Purpose

    For bacterial expression of His-tagged human YTHDF2

    Depositor

    Samie Jaffrey

    Article

    Patil et al Nature. 2016 Sep 7;537(7620):369-373. do

    Insert

    YTHDF2 (YTHDF2 Human)
    

    Use

    Tags

    6X HIS

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pJG103

    Plasmid

    #158533

    Purpose

    wrmScarlet11 x3 tandem repeats

    Depositor

    Cynthia Kenyon

    Article

    Goudeau et al Genetics. 2021 Apr 15;217(4). pii: 61264

    Insert

    wrmScarlet11 x3 tandem repeats
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pOPINK-OTUD2 (full-length, aa 1-348)

    Plasmid

    #61409

    Purpose

    Expresses human OTUD2 (full-length) in E. coli.

    Depositor

    David Komander

    Article

    Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

    Insert

    OTUD2 (YOD1 Human)
    

    Use

    Tags

    His6-GST-3C

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDule-Tet3.0

    Plasmid

    #174080

    Purpose

    Tetrazine 3.0 tRNA synthetase and cognate amber suppressing tRNA derived from M. barkeri for expression in E.coli.

    Depositor

    Ryan Mehl

    Article

    Jang et al J Am Chem Soc. 2020 Apr 22;142(16):7245-

    Insert

    Tet3.0 tRNA synthetase and tRNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    L270G, Y271L, N311G, C313A

    Promoter

    Available since

    Jan. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCDB179-BhrPETase

    Plasmid

    #218692

    Purpose

    Encodes codon optimized BhrPETase enzyme for bacterial expression

    Depositor

    Gregg Beckham

    Article

    Norton-Baker et al Sci Rep. 2024 Jun 24;14(1):14449. doi: 1

    Insert

    BhrPETase
    

    Use

    Tags

    10xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pBad33_ISYmu1_reRNA

    Plasmid

    #205358

    Purpose

    Bacterial expression of ISYmu1 TnpB and its reRNA

    Depositor

    Haoyi Wang

    Article

    Xiang et al Nat Biotechnol. 2023 Jun 29. doi: 10.103

    Insert

    ISYmu1 TnpB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pRSFDuet-1_VNp-LZ_VNp-LZ-mNeongreen-Etanercept

    Plasmid

    #182424

    Purpose

    Bacterial expression of Vesicle Nucleating peptide-Leucine Zipper and Vesicle Nucleating peptide-Leucine Zipper-mNeongreen-Etanercept fusion proteins

    Depositor

    Dan Mulvihill

    Article

    Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

    Inserts

    VNp-LZ-mNeongreen-Etanercept (TNFRSF1B Human)
    VNp-LZ
    

    Use

    Tags

    Vesicle Nucleating peptide (VNp), and Vesicle Nuc…

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge