30,695 results

1. pTXB1-Tn5

   Plasmid

   #60240

   Purpose

   IPTG-inducible expression of Transposon Tn5 fused to Mxe Intein and Chitin-binding domain.

   Depositor

   Rickard Sandberg

   Article

   Picelli et al Genome Res. 2014 Jul 30. pii: gr.177881.

   Insert

   tnpA from pBam1
   

   Tags

   Mxe intein - Chitin-binding domain

   Expression

   Bacterial

   Mutation

   E54K, L372P

   Promoter

   T7 promoter

   Available Since

   Nov. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSAM_Ec

   Plasmid

   #102939

   Purpose

   Transposon mutagenesis and Tn-seq

   Depositor

   Matthew Mulvey

   Article

   Wiles et al PLoS Genet. 2013;9(8):e1003716. doi: 10.

   Insert

   mariner transposon flanked by MmeI modified inverted repeats and the himar1C9 transposase
   

   Expression

   Bacterial

   Available Since

   April 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pACRISPR

   Plasmid

   #113348

   Purpose

   A sgRNA expression plasmid for genome editing in Pseudomonas aeruginosa

   Depositor

   Quanjiang Ji

   Article

   Chen et al iScience. 2018 Aug 31;6:222-231. doi: 10

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology

   Expression

   Bacterial

   Available Since

   Aug. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRBC-sfGFP-150TAG

   Plasmid

   #174076

   Purpose

   Plasmid with p15a origin of replication expressing codon optimized superfolder GFP-150 TAG with C-terminal His6 tag, under T7 promoter,

   Depositor

   Ryan Mehl

   Article

   Zhu et al ACS Chem Biol. 2019 Jul 19;14(7):1564-15

   Insert

   Superfolder GFP
   

   Tags

   His-6

   Expression

   Bacterial

   Mutation

   N150TAG

   Promoter

   T7

   Available Since

   Nov. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCre

   Plasmid

   #236212

   Purpose

   Arabinose-inducible Cre recombinase

   Depositor

   Matthew Waldor

   Article

   Basta et al Nat Microbiol. 2025 Mar 27. doi: 10.1038

   Insert

   Cre recombinase
   

   Expression

   Bacterial

   Available Since

   April 8, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRBC-sfGFP wild type

   Plasmid

   #174075

   Purpose

   Plasmid with p15a origin of replication expressing codon optimized superfolder GFP with C-terminal His6 tag, under T7 promoter

   Depositor

   Ryan Mehl

   Article

   Zhu et al ACS Chem Biol. 2019 Jul 19;14(7):1564-15

   Insert

   Superfolder GFP
   

   Tags

   His-6

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. SpyCatcher003-sfGFP

   Plasmid

   #133449

   Purpose

   Expresses SpyCatcher003-superfolder GFP in bacterial cytoplasm

   Depositor

   Mark Howarth

   Article

   Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. p

   Insert

   SpyCatcher003-sfGFP
   

   Tags

   His6 and TEV site

   Expression

   Bacterial

   Promoter

   T5

   Available Since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. PCV-Cas9

   Plasmid

   #123643

   Purpose

   Expresses Cas9 with an N-terminal HUH-tag called PCV2

   Depositor

   Wendy Gordon

   Article

   Aird et al Commun Biol. 2018 May 31;1:54. doi: 10.1

   Inserts

   PCV2 (ORF1 )
   Cas9
   

   Tags

   (EAAAK)8 linker and His6 + SUMO

   Expression

   Bacterial

   Mutation

   2-116

   Available Since

   March 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. ACS1

   Plasmid

   #13746

   Depositor

   John Denu

   Article

   Hallows et al Proc Natl Acad Sci U S A. 2006 Jul 5. 10

   Insert

   Acetyl CoA Synthetase 1 (Acss2 Mouse)
   

   Tags

   His

   Expression

   Bacterial

   Available Since

   Feb. 23, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBAD-mTagBFP2

    Plasmid

    #34632

    Depositor

    Vladislav Verkhusha

    Article

    Subach et al PLoS One. 2011;6(12):e28674. Epub 2011 D

    Insert

    mTagBFP2
    

    Expression

    Bacterial

    Mutation

    I174A compared to mTagBFP (enhanced photostabilit…

    Available Since

    Jan. 13, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTNS3

    Plasmid

    #63127

    Purpose

    Tn7 transposase expression

    Depositor

    Herbert Schweizer

    Article

    Choi et al Appl Environ Microbiol. 2008 Feb;74(4):1

    Type

    Empty backbone

    Expression

    Bacterial

    Promoter

    P1 and Ptac

    Available Since

    May 17, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pET21b(+)-Is-PETase

    Plasmid

    #112202

    Purpose

    pET-21b(+) based plasmid for expression of PETase from Ideonella sakaiensis 201-F6 (Genbank GAP38373.1), codon optimized for expression in E. coli K12

    Depositor

    Gregg Beckham, Christopher Johnson

    Article

    Austin et al Proc Natl Acad Sci U S A. 2018 May 8;115

    Insert

    PETase gene from Ideonella sakaiensis 201-F6, codon optimized for expression in E. coli K12
    

    Tags

    6XHIS

    Expression

    Bacterial

    Promoter

    N/A

    Available Since

    July 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. TDP43-NTD

    Plasmid

    #241024

    Purpose

    Bacterial expression of TDP-43 NTD

    Depositor

    Nicolas Fawzi

    Insert

    TDP43-NTD
    

    Tags

    6x His

    Expression

    Bacterial

    Mutation

    changed N81 to stop codon

    Available Since

    July 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRK793

    Plasmid

    #8827

    Depositor

    David Waugh

    Article

    Kapust et al Protein Eng. 2001 Dec . 14(12):993-1000.

    Insert

    TEV protease, S219V mutant
    

    Tags

    His, MBP (with TEV), and polyarginine

    Expression

    Bacterial

    Mutation

    S219V mutation (improves stability)

    Available Since

    May 24, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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15. pRMC2

    Plasmid

    #68940

    Purpose

    tetracycline-inducible expression vector for Staphylococcus aureus

    Depositor

    Tim Foster

    Article

    Corrigan et al Plasmid. 2009 Mar;61(2):126-9. doi: 10.1

    Type

    Empty backbone

    Use

    Tet inducible e. coli/staphylococcus shuttle vect…

    Expression

    Bacterial

    Available Since

    Nov. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. mTagBFP2-pBAD

    Plasmid

    #54572

    Purpose

    Localization: Protein Expression Vector , Excitation: 399, Emission: 456

    Depositor

    Michael Davidson

    Article

    Subach et al PLoS One. 2011;6(12):e28674. Epub 2011 D

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Tags

    6xHis, T7, and mTagBFP2

    Expression

    Bacterial

    Available Since

    July 25, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. TDP-43_NTD1-80_E17R

    Plasmid

    #104479

    Purpose

    bacterial expression of TDP-43 NTD E17R

    Depositor

    Nicolas Fawzi

    Article

    Wang et al EMBO J. 2018 Mar 1;37(5). pii: embj.2017

    Insert

    TDP-43 (TARDBP Human)
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    E17R

    Available Since

    March 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. BirA pLEMO

    Plasmid

    #119818

    Purpose

    Express endogenous biotin ligase in E.coli

    Depositor

    Alexander Gabibov

    Article

    Lomakin et al Bioconjug Chem. 2019 Apr 25. doi: 10.102

    Insert

    Biotin ligase
    

    Expression

    Bacterial

    Promoter

    Rhamnose promoter

    Available Since

    April 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pNP1950

    Plasmid

    #222729

    Purpose

    J23119-BsaI_bRNA Golden Gate Landing Pad-HDV; T7-IS621 Recombinase

    Depositor

    Patrick Hsu

    Article

    Durrant et al Nature. 2024 Jun;630(8018):984-993. doi:

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    July 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSL1145 (pSPIN, pBBR1 backbone, R*MmeI)

    Plasmid

    #160736

    Purpose

    Single-plasmid V. cholerae CAST, encodes all proteins, crRNA, and donor DNA. Non-targeting crRNA with BsaI sites for spacer cloning. Mini-tn has MmeI site in R end for Tn-seq. pBBR1 backbone.

    Depositor

    Samuel H. Sternberg

    Article

    Vo et al Nat Biotechnol. 2020 Nov 23. pii: 10.103

    Insert

    VchCAST proteins, crRNA, and donor DNA
    

    Expression

    Bacterial

    Promoter

    J23119

    Available Since

    March 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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