32,161 results

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pUCIDT-attL1-Human ABeta-attR5

Plasmid

#160436

Purpose

Entry vector for cloning human Amyloid Beta(1-42) using 2-fragment gateway recombination

Depositor

Nicholas Tolwinski

Article

Kaur et al Bio Protoc. 2020 Dec 5;10(23):e3856. doi

Insert

Homo sapiens amyloid beta precursor protein (APP Human)

Expression

Bacterial

Available Since

Oct. 6, 2021

Availability

Academic Institutions and Nonprofits only

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MBP-His6-TEV-MMS2

Plasmid

#25465

Depositor

Cynthia Wolberger

Article

Eddins et al Nat Struct Mol Biol. 2006 Oct . 13(10):9

Insert

MMS2 (MMS2, UBC13 Budding Yeast)

Tags

His6 and MBP

Expression

Bacterial

Available Since

July 21, 2010

Availability

Academic Institutions and Nonprofits only
pBAD33MevT

Plasmid

#17814

Depositor

Jay Keasling

Article

Martin et al Nat Biotechnol. 2003 Jul . 21(7):796-802

Insert

MevT

Use

Synthetic Biology

Expression

Bacterial

Available Since

May 16, 2008

Availability

Academic Institutions and Nonprofits only

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pGEX-2T-GST-RNMT

Plasmid

#112709

Purpose

Expresses N-terminally GST-tagged human RNMT in bacterial cells

Depositor

Daniel Schoenberg

Article

Trotman et al Nucleic Acids Res. 2017 Oct 13;45(18):10

Insert

RNMT (RNMT Human)

Tags

GST and thrombin cleavage site

Expression

Bacterial

Available Since

Aug. 9, 2019

Availability

Academic Institutions and Nonprofits only

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ftsZ-RFP

Plasmid

#63842

Purpose

This plasmid is designed as a bacterial cell division sensor

Depositor

Howard Salis

Insert

ftsZ, mRFP1 and GFPmut3b

Tags

mRFP1

Expression

Bacterial

Mutation

Gene is translationally fused to RFP

Available Since

March 27, 2020

Availability

Academic Institutions and Nonprofits only

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pJUMP46-2A(sfGFP)

Plasmid

#126990

Purpose

Level 2 vector. sfGFP reporter. Origin p15A (medium copy number)

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

July 2, 2019

Availability

Academic Institutions and Nonprofits only

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pET11d_Rev

Plasmid

#119322

Purpose

Expression of protein Rev from HIV

Depositor

Francisco Blanco, Robert Tycko

Article

Blanco et al J Mol Biol. 2001 Nov 2;313(4):845-59. do

Insert

Rev (rev HIV B1)

Expression

Bacterial

Available Since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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NylB'-SCY_pET21b

Plasmid

#215417

Purpose

Expression construct for NylB'-SCY gene, codon optimized for expression in E. coli

Depositor

Gregg Beckham

Article

Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

Insert

NylB'-SCY

Expression

Bacterial

Mutation

R187S/F264C/D370Y

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pTriEx-mCherry-LOVSD

Plasmid

#81033

Purpose

Mammalian expression of the super-dark state of LOV2

Depositor

Klaus Hahn

Article

Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

Insert

LOVSD

Tags

mCherry

Expression

Bacterial, Insect, and Mamm…

Mutation

C450A, L514K, G528A, L531E, N538E

Available Since

Aug. 17, 2016

Availability

Academic Institutions and Nonprofits only

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pTHSSe_53

Plasmid

#109251

Purpose

pUC BbsI cloning vector

Depositor

Christopher Voigt

Article

Segall-Shapiro et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

Insert

sfGFP

Expression

Bacterial

Promoter

PJ23105

Available Since

May 29, 2019

Availability

Academic Institutions and Nonprofits only

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