33,902 results
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Plasmid#132695PurposepRDV5 plasmid containing a loop Sybody for the generation of a qPCR standard curveDepositorInsertSb_loop including 5' and 3' flanking sequences used for ribosome display
UseSynthetic BiologyExpressionBacterialPromoterT7Available sinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTOX8
Plasmid#127453PurposeEncodes MqsR toxin and tsPurple chromoprotein. CAM-RDepositorInsertsmqsR toxin
tsPurple chromoprotein
ExpressionBacterialMutationCodon optimized from protein sequence in FPbasePromoterapFAB46 and pRhaAvailable sinceJuly 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pS448∙CsR
Plasmid#122096PurposeCRISPR/Cas9 counterselection in Gram-negative bacteriaDepositorInsertsCas9
sgRNA
UseSynthetic BiologyExpressionBacterialPromoterPem7 and PmAvailable sinceApril 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pIA33
Plasmid#120803PurposeE. coli-C. difficile shuttle vector for CRISPR interference (CRISPRi) in C. difficile; sgRNA targets rfp; Pxyl::dCas9-opt Pgdh::sgRNA-rfpDepositorInsertsdeactivated nuclease dCas9, codon-optimized for C. difficile
single guide RNA targeting red fluorescent protein
UseCRISPRExpressionBacterialMutationBase-pairing region targets red fluorescent prote…PromoterPgdh and PxylAvailable sinceFeb. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
HHM-iATPSnFR1.0
Plasmid#102546Purposeexpresses ATP sensor in bacteriaDepositorInsertiATPSnFR1.0
ExpressionBacterialAvailable sinceJan. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJMP1337
Plasmid#119270Purposefor cloning new sgRNAsDepositorInsertdcas9
ExpressionBacterialMutationD10A and H840AAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRSF-MCM3/5
Plasmid#116950PurposeExpresses human MCM3 and human MCM5DepositorAvailable sinceNov. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMRE-Tn7-132
Plasmid#118550PurposeminiTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteriaDepositorInsertsGFP2
UseMinitn7 delivery vectorExpressionBacterialAvailable sinceNov. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEX6p-1-FRB-PafA
Plasmid#113404PurposeExpresses FRB-PafA in bacteriaDepositorInsertFRB-PafA
TagsGSTExpressionBacterialPromotertacAvailable sinceAug. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEX6p-1-BCCP-PupE
Plasmid#113403PurposeExpresses BCCP-PupE in bacteriaDepositorInsertBCCP-PupE
TagsGSTExpressionBacterialPromotertacAvailable sinceAug. 16, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pET-SUMO-mGSDMD
Plasmid#111560PurposeExpress mouse GSDMD in E.coliDepositorAvailable sinceJune 27, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pBP-T7RNAP
Plasmid#74096PurposeT7 RNA polymerase PCR cloned from BL21 (DE3) genomic DNADepositorPublicationInsertT7 RNAP
UseSynthetic BiologyExpressionBacterialPromoterNoneAvailable sinceApril 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pT7-7 asyn E46K
Plasmid#105728PurposeBacterial expression of mutant human alpha synucleinDepositorAvailable sinceApril 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pETDuet_huCAD_ICADL (TevS)
Plasmid#100098Purposedual expression of human CAD and ICAD. The two caspase cleavage sites in ICAD were mutated to TEVP cleavable sequencesDepositorExpressionBacterialMutationtwo caspase cleavage sites (DETD-117 and DAVD-224…PromoterT7Available sinceJan. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEB1-SCFP3A
Plasmid#103970PurposePlasmid encoding SCFP3ADepositorInsertSCFP3A
UseLow copyExpressionBacterialMutationMutations relative to wild-type GFP (F64L, S65T, …PromoterproCAvailable sinceDec. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pet28a-His6-Keap1
Plasmid#62454PurposeHis6 tagged Keap1 for E.coli expressionDepositorPublicationAvailable sinceNov. 27, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSCB-YFP
Plasmid#101737PurposeThe plasmid can be either plasmid propogation backbone or expression vector in specific strainDepositorTypeEmpty backboneUseSynthetic BiologyTagseYFPExpressionBacterialPromoterTrc1OAvailable sinceOct. 4, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pC018 - LshCas13a from Leptotrichia shahii
Plasmid#91908PurposeExpresses LshCas13a for bacterial expression and contains backbone for spacer cloningDepositorInsertLshCas13a and guide expression cassette
ExpressionBacterialAvailable sinceOct. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pQE80L-SpyCatcher-ELP-GFP
Plasmid#69835PurposeBacterial expression plasmid containing SpyCatcher-ELP-GFP fusion. SpyCatcher forms a covalent bond with SpyTag and can be used to label plasma membrane localized SpyTag-C1C2 in live cells.DepositorInsertSpyCatcher-ELP-GFP
Tags6x His Tag, GFP, and TEV TagExpressionBacterialPromoterT5 promoter/lac operator elementAvailable sinceJuly 19, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pKD236-4b
Plasmid#90956PurposeExpresses ThsS constitutivelyDepositorInsertThsS
ExpressionBacterialPromoterBba_J23104Available sinceMay 16, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by