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pJExpress401-KDAC8

Plasmid

#224208

Purpose

Expresses human KDAC8 (HDAC8) in E. coli

Depositor

Terry Watt

Article

Toro et al Protein Sci. 2015 Dec;24(12):2020-32. do

Insert

KDAC8 (HDAC8 Human)

Tags

TEV-cleavable His6

Expression

Bacterial

Promoter

T5

Available Since

Dec. 6, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pJExpress401-KDAC4 CD

Plasmid

#224251

Purpose

Expresses human KDAC4 (HDAC4) catalytic domain in E. coli

Depositor

Terry Watt

Article

Toro et al Protein Expr Purif. 2018 Jan;141:1-6. do

Insert

KDAC4 (HDAC4 Human)

Tags

TEV-cleavable His6

Expression

Bacterial

Mutation

Only includes residues 648-1083 (catalytic domain…

Promoter

T5

Available Since

Oct. 22, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pJExpress401-KDAC7 CD

Plasmid

#224341

Purpose

Expresses human KDAC7 (HDAC7) catalytic domain in E. coli

Depositor

Terry Watt

Article

Toro et al Protein Expr Purif. 2018 Jan;141:1-6. do

Insert

KDAC7 (HDAC7 Human)

Tags

TEV-cleavable His6

Expression

Bacterial

Mutation

Only includes residues 521-942 (catalytic domain).

Promoter

T5

Available Since

Oct. 22, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pRAF-MutS

Plasmid

#86605

Purpose

E. coli MutS expression plasmid

Depositor

Richard Fishel

Article

Liu et al Nature. 2016 Nov 16;539(7630):583-587. d

Insert

E. coli MutS (D835R,R840E)

Tags

his-tag and ald-tag

Expression

Bacterial

Mutation

D835R, R840E

Promoter

T7

Available Since

Feb. 8, 2017

Availability

Academic Institutions and Nonprofits only

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pEB1-coSCFP3A

Plasmid

#104015

Purpose

Plasmid encoding coSCFP3A

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

coSCFP3A

Use

Low copy

Expression

Bacterial

Mutation

Same protein sequence as SCFP3A (codon optimized …

Promoter

proC

Available Since

Dec. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pCDF-NtXNtS1R1R2B2

Plasmid

#160882

Purpose

Expression of N. tabacum Rubisco small subunit (rbcS-S1), Rubisco chaperone protiens rbcX, raf1, bsd2 and raf2 in E. coli.

Depositor

Maureen Hanson

Article

Lin et al Nat Plants. 2020 Oct;6(10):1289-1299. do

Insert

rbcS-S1, rbcX, raf1, raf2, bsd2

Expression

Bacterial

Promoter

rbcS-S1: T7, chaperones: T7

Available Since

Dec. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pLBH531_MBP-Cas14a1 expression

Plasmid

#112500

Purpose

Expresses MBP fused to Cas14a1

Depositor

Jennifer Doudna

Article

Harrington et al Science. 2018 Oct 18. pii: science.aav42

Insert

Cas14a1

Use

CRISPR

Tags

10xHis-MBP

Expression

Bacterial

Promoter

T7

Available Since

Oct. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pET21a(+)-Bst LF-6xHis

Plasmid

#159148

Purpose

expresses His-tagged large fragment of Bst polymerase

Depositor

Andrea Pauli

Article

Kellner et al Front Mol Biosci. 2022 Apr 1;9:801309. d

Insert

large fragment of Geobacillus stearothermophilus DNA polymerase I

Tags

His

Expression

Bacterial

Available Since

Sept. 1, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pDB-His-MBP-mGSDMD

Plasmid

#123365

Purpose

Expression of full-length mouse GSDMD in E. coli

Depositor

Hao Wu

Article

Orning et al Science. 2018 Nov 30;362(6418):1064-1069

Insert

GSDMD (Gsdmd Mouse)

Tags

His6-MBP

Expression

Bacterial

Promoter

T7

Available Since

May 3, 2019

Availability

Academic Institutions and Nonprofits only

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5KO9 (HMCES)

Plasmid

#101877

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Cheryl Arrowsmith

Insert

HMCES (HMCES Human)

Tags

6x His (modified C-term tag is AENLYFQSHHHHHH)

Expression

Bacterial

Promoter

T7

Available Since

April 30, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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pFA6a-mEOS3.2-KanMX6

Plasmid

#87030

Purpose

Tag S. pombe genes in C terminal with mEOS3.2

Depositor

Julien Berro, Thomas Pollard

Article

Laplante et al Proc Natl Acad Sci U S A. 2016 Oct 4;113

Insert

mEOS3.2

Expression

Bacterial and Yeast

Available Since

Feb. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pBB550

Plasmid

#27396

Depositor

Bernd Bukau

Article

de Marco et al BMC Biotechnol. 2007 Jun 12;7:32. doi: 1

Insert

dnaK, dnaJ, GroESL

Expression

Bacterial

Available Since

Sept. 7, 2012

Availability

Academic Institutions and Nonprofits only

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R701-X65-527: His6-tev-Hs.CALM1(2-148)

Plasmid

#159693

Purpose

E. coli protein expression of His6-tev-Hs.CALM1(2-148)

Depositor

Dominic Esposito

Article

Agamasu et al Biophys J. 2019 Mar 19;116(6):1049-1063.

Insert

His6-tev-Hs.CALM1(2-148) (CALM1 Human)

Tags

His6-tev

Expression

Bacterial

Available Since

Oct. 5, 2020

Availability

Academic Institutions and Nonprofits only

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pS24·Pm-GFP

Plasmid

#122594

Purpose

Expresses GFP under the regulatory control of Pm. Expression needs additional xyls gene

Depositor

Pablo Ivan Nikel

Article

Volke et al Microb Biotechnol. 2019 Mar 12. doi: 10.

Insert

Pm promoter

Use

Synthetic Biology

Expression

Bacterial

Promoter

Pm

Available Since

March 22, 2019

Availability

Academic Institutions and Nonprofits only

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pCas9

Plasmid

#167547

Purpose

Used for CRISPR-Cas9 mediated recombineering in Enterococcus. Can clone desired gRNA using BsaI digestion.

Depositor

Howard Hang

Article

Chen et al Appl Environ Microbiol. 2021 Jul 7:AEM00

Inserts

chloramphenicol acetyl transferase
pUC19 origin of replication

Use

CRISPR

Expression

Bacterial

Available Since

June 28, 2021

Availability

Academic Institutions and Nonprofits only

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pET15TV-L-hlPYK WT

Plasmid

#187975

Purpose

Expresses the human liver pyruvate kinase

Depositor

Audrey Lamb

Article

McFarlane et al Acta Crystallogr F Struct Biol Commun. 2

Insert

hlPYK WT

Tags

Hexahistag

Expression

Bacterial

Promoter

T7

Available Since

Aug. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pET24a isPETase

Plasmid

#225759

Purpose

Expresses codon-optimized PETase from Ideonella sakaiensis in E. coli without native signal sequence and with a C-terminal his-tag

Depositor

Jason Boock

Article

Carter et al Microb Cell Fact. 2024 Nov 25;23(1):319.

Insert

isPETase

Tags

6xHis Tag

Expression

Bacterial

Promoter

T7

Available Since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pET-DUET-1-mRhubarb719-HO

Plasmid

#141200

Purpose

Expresses mRhubarb719 fluorescent protein and heme oxygenase

Depositor

Elad Firnberg

Article

Rogers et al Sci Rep. 2019 Oct 30;9(1):15653. doi: 10

Inserts

mRhubarb719
heme oxygenase

Tags

6x-His

Expression

Bacterial

Mutation

R134H, Y198S, T202Y, V203I, W309R, Q310A compared…

Promoter

T7

Available Since

Aug. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pMBP-hLIF

Plasmid

#179711

Purpose

Expresses H6-MBP-LIF

Depositor

Mark Arbing

Insert

Leukemia inhibitory factor (LIF Human)

Tags

His6-MBP-TEV protease site

Expression

Bacterial

Promoter

tac

Available Since

Jan. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pET-Expotin 1

Plasmid

#42047

Depositor

Kumiko Ui-Tei

Article

Nishi et al RNA. 2013 Jan;19(1):17-35. doi: 10.1261/

Insert

Exportin-1 (XPO1 Human)

Tags

His

Expression

Bacterial

Available Since

Feb. 25, 2013

Availability

Academic Institutions and Nonprofits only

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