30,738 results

1. pET15b His-p97

   Plasmid

   #169020

   Purpose

   Expresses His-p97 in bacteria

   Depositor

   Hemmo Meyer

   Article

   Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

   Insert

   p97
   

   Tags

   His

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   July 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pEB2-mScarlet

   Plasmid

   #104006

   Purpose

   Plasmid encoding mScarlet

   Depositor

   Philippe Cluzel

   Article

   Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

   Insert

   mScarlet
   

   Use

   Low copy

   Expression

   Bacterial

   Mutation

   Mutations relative to DsRed (MSKGE AVIKEF… N6A, R…

   Promoter

   proC

   Available Since

   Dec. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pET20b_Pa-PiuA

   Plasmid

   #128955

   Purpose

   expresses Pseudomonas aeruginosa PiuA in E. coli

   Depositor

   James Naismith

   Article

   Moynie et al Antimicrob Agents Chemother. 2017 Mar 24

   Insert

   iron transport outer membrane receptor (PiuA) (PA4514 Pseudomonas aeruginosa PAO1)
   

   Tags

   6xHis and pelB leader

   Expression

   Bacterial

   Mutation

   Signal peptide sequence has been removed

   Promoter

   T7

   Available Since

   Aug. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCold-TF-hRubicon

   Plasmid

   #64147

   Purpose

   For protein expression in E. coli

   Depositor

   Tamotsu Yoshimori

   Article

   Tabata et al Mol Biol Cell. 2010 Dec;21(23):4162-72.

   Insert

   Rubicon (RUBCN Human)
   

   Tags

   6xHis-tag and Trigger Factor (TF)

   Expression

   Bacterial

   Available Since

   April 17, 2015

   Availability

   Academic Institutions and Nonprofits only

5. sgRNA_expression_vector

   Plasmid

   #210212

   Purpose

   An empty gRNA expression vector to clone U6 promoter driven sgRNAs with gRNA scaffold and with co-expression of DsRed. sgRNA can be cloned by Golden Gate Assembly or restriction digestion using BbsI.

   Depositor

   Albert Jeltsch

   Article

   Rajaram et al Methods Mol Biol. 2024;2842:179-192. doi

   Type

   Empty backbone

   Expression

   Bacterial and Mammalian

   Promoter

   U6

   Available Since

   Dec. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pREDTAI

   Plasmid

   #51627

   Purpose

   E coli Genetic Modification

   Depositor

   Sheng Yang

   Article

   Yang et al Appl Environ Microbiol. 2014 Jul 1;80(13

   Insert

   I-SceI (SCEI Budding Yeast)
   

   Use

   Red recombinase and i-scei endonuclease

   Expression

   Bacterial

   Promoter

   trc

   Available Since

   May 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. Pet28a_Human_H3.3

   Plasmid

   #42632

   Depositor

   Joe Landry

   Insert

   Human H3.3 (H3-3A Human)
   

   Tags

   Untagged

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Feb. 25, 2013

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

8. pSB219

   Plasmid

   #164125

   Purpose

   p15A backbone with PhlF binding sites

   Depositor

   Christopher Voigt

   Article

   Shao et al Nat Commun. 2021 Mar 5;12(1):1475. doi:

   Insert

   sfGFP
   

   Expression

   Bacterial

   Promoter

   J23105

   Available Since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCAD39_mIRG1_4-462_pvp008

   Plasmid

   #124873

   Purpose

   Expresses mouse CAD (Irg1) in E.coli

   Depositor

   Konrad Buessow

   Article

   Chen et al Proc Natl Acad Sci U S A. 2019 Sep 23. p

   Insert

   cis-aconitate decarboxylase (Acod1 Mouse)
   

   Tags

   StrepTag and TEV site

   Expression

   Bacterial

   Mutation

   deleted amino acids 1-3 and 463-end.

   Promoter

   T7

   Available Since

   Sept. 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pQTEV-HSPB8

    Plasmid

    #34710

    Depositor

    Konrad Buessow

    Article

    Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

    Insert

    heat shock 22 kDa protein 8 (HSPB8 Human)
    

    Tags

    His and TEV

    Expression

    Bacterial

    Available Since

    March 22, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pTAMAHISTEV_PfeA

    Plasmid

    #128943

    Purpose

    expresses PfeA in E. coli

    Depositor

    James Naismith

    Article

    Moynie et al Nat Commun. 2019 Aug 14;10(1):3673. doi:

    Insert

    ferric enterobactin receptor
    

    Tags

    TEV protease cleavable 7xHis and TamA

    Expression

    Bacterial

    Mutation

    Signal peptide sequence has been removed (1-75)

    Promoter

    T7

    Available Since

    Aug. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pET28a-cMSPdH5

    Plasmid

    #199771

    Purpose

    Expresses split-intein fused MSPdH5 for circularization during expression (external His-Tag)

    Depositor

    Franz Hagn

    Article

    Miehling et al Chembiochem. 2018 Sep 17;19(18):1927-193

    Insert

    cMSPdH5
    

    Tags

    His6-Tag

    Expression

    Bacterial

    Mutation

    external His-Tags flanking the C and N termini

    Available Since

    Jan. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAB213

    Plasmid

    #101677

    Purpose

    mCherry under T7 promoter control

    Depositor

    Mustafa Khammash

    Article

    Baumschlager et al ACS Synth Biol. 2017 Nov 17;6(11):2157-2

    Insert

    mCherry under T7 promoter control
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    Nov. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCRISPathBrick

    Plasmid

    #65006

    Purpose

    E. coli vector for expression of S. pyogenes dCas9, tracrRNA, and nontargeting CRISPR array with BsaI site for inserting user-defined spacer-repeat bricks

    Depositor

    Mattheos Koffas

    Article

    Cress et al ACS Synth Biol. 2015 Mar 30.

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Expression

    Bacterial

    Promoter

    Constitutive native promoters

    Available Since

    May 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pMCRW1

    Plasmid

    #122462

    Purpose

    Human NPM-ALK fusion protein gene in pET28a bacterial expression plasmid. Production of recombinant human NPM-ALK fusion protein in E. coli Bl21 Rosetta2 cells

    Depositor

    Richard C. Willson

    Article

    Kourentzi et al Sci Rep. 2020 Mar 19;10(1):5078. doi: 10

    Insert

    NPM-ALK fusion protein (ALK, NPM1 Human)
    

    Tags

    His Tag

    Expression

    Bacterial

    Promoter

    T7lac promoter

    Available Since

    May 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pDEST17-UbE2A

    Plasmid

    #15780

    Depositor

    Wade Harper

    Article

    Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

    Insert

    Ubiquitin conjugating enzyme UbE2A (UBE2A Human)
    

    Tags

    His

    Expression

    Bacterial

    Available Since

    Sept. 28, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET21a-LysRS-His

    Plasmid

    #124114

    Purpose

    Bacterial expression plasmid for PURE system components

    Depositor

    Sebastian Maerkl, Takuya Ueda

    Article

    Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

    Insert

    Lysine-tRNA ligase (lysU Escherichia coli)
    

    Tags

    6x-His

    Expression

    Bacterial

    Available Since

    April 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pRK172-α-syn-TEV-GFP

    Plasmid

    #166671

    Purpose

    Bacterial expression of alpha synuclein coupled to GFP via a TEV site for uptake studies

    Depositor

    Iris Lindberg

    Article

    Jarvela et al PLoS One. 2021 Jan 26;16(1):e0241161. do

    Insert

    mouse synuclein (from V. Lee) (Snca Mouse)
    

    Tags

    GFP

    Expression

    Bacterial

    Mutation

    added a TEV cleavage site sequence between synucl…

    Promoter

    CMV

    Available Since

    July 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAJM.773

    Plasmid

    #108527

    Purpose

    VanRAM + PVanCC-YFP Reporter. Van sensor for use in non-Marionette strain.

    Depositor

    Christopher Voigt

    Article

    Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

    Insert

    VanRAM + PVanCC-YFP
    

    Expression

    Bacterial

    Available Since

    July 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBAD-GINKO1

    Plasmid

    #113111

    Purpose

    Bacterial expression of genetically encoded green fluorescent potassium ion indicator GINKO1

    Depositor

    Robert Campbell

    Article

    Shen et al Commun Biol. 2019 Jan 14;2:18. doi: 10.1

    Insert

    GINKO1
    

    Tags

    6-His Tag, T7 tag (gene 10 leader), and Xpress™ t…

    Expression

    Bacterial

    Promoter

    araBAD

    Available Since

    Aug. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge