4,931 results

1. pFL His-I3

   Plasmid

   #113513

   Purpose

   Expression of His-I3 in insect cells (Baculovirus system)

   Depositor

   Hemmo Meyer

   Article

   Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

   Insert

   I3 (PPP1R11 Human)
   

   Tags

   6*His

   Expression

   Insect

   Available Since

   Oct. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pVL-FLAG/His LCOR

   Plasmid

   #114450

   Purpose

   Insect baculovirus expression vector containing N terminal FLAG/His tagged human LCOR coding sequence

   Depositor

   Adrian Bracken

   Article

   Conway et al Mol Cell. 2018 May 3;70(3):408-421.e8. d

   Insert

   LCOR (LCOR Human)
   

   Tags

   FLAG-His

   Expression

   Insect

   Available Since

   Sept. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pFastBac-GST-Mps1-KD

   Plasmid

   #114066

   Purpose

   protein production

   Depositor

   Geert Kops

   Article

   Jelluma et al PLoS One. 2008 Jun 11;3(6):e2415. doi: 1

   Insert

   Mps1-KD (TTK Human)
   

   Tags

   GST

   Expression

   Insect

   Mutation

   Mps1 KD (D664A)

   Available Since

   Sept. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFastBac-GST-Mps1-WT

   Plasmid

   #114065

   Purpose

   protein production

   Depositor

   Geert Kops

   Article

   Jelluma et al PLoS One. 2008 Jun 11;3(6):e2415. doi: 1

   Insert

   Mps1-WT (TTK Human)
   

   Tags

   GST

   Expression

   Insect

   Mutation

   none

   Available Since

   Sept. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pOEM1_pCMV:axFgf8-pPH:vsvged

   Plasmid

   #111159

   Purpose

   Baculovirus rescue vector, VSVGED pseudotype, hShh

   Depositor

   Elly Tanaka

   Article

   Nacu et al Nature. 2016 May 19;533(7603):407-10. do

   Insert

   axFgf8
   

   Use

   Baculoviral rescue shuttle vector

   Expression

   Insect and Mammalian

   Mutation

   wt

   Promoter

   CMV

   Available Since

   Aug. 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFastBac-LacI-LAP-HEC1

   Plasmid

   #114033

   Purpose

   Production of baculovirus with LacI-LAP-HEC1

   Depositor

   Geert Kops

   Article

   Hiruma et al Science. 2015 Jun 12;348(6240):1264-7. d

   Insert

   LacI-LAP-HEC1 (NDC80 Human)
   

   Expression

   Insect

   Mutation

   none

   Promoter

   CMV

   Available Since

   Aug. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pFastBac Dual PNG172-STII PLU-HIS

   Plasmid

   #112992

   Purpose

   Baculovirus expression of Drosophila png172-Flag and plu-His

   Depositor

   Terry Orr-Weaver

   Inserts

   png172 (png Fly)
   plu (plu Fly)
   

   Tags

   Flag and His

   Expression

   Insect

   Mutation

   png172 mutation

   Available Since

   Aug. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pFastBac Dual PNG-STII PLU-HIS

   Plasmid

   #112993

   Purpose

   Baculovirus expression of Drosophila png-Flag and plu-His

   Depositor

   Terry Orr-Weaver

   Inserts

   png (png Fly)
   plu (plu Fly)
   

   Tags

   Flag and His

   Expression

   Insect

   Available Since

   Aug. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pOEM1_pCMV:deltaN-bmp4-pPH:vsvged

   Plasmid

   #111158

   Purpose

   Baculovirus rescue vector, VSVGED pseudotype, hShh

   Depositor

   Elly Tanaka

   Article

   Wagner et al Dev Cell. 2017 Mar 27;40(6):608-617.e6.

   Insert

   deltaNhBmp4 (BMP4 Human)
   

   Use

   Baculoviral rescue shuttle vector

   Expression

   Insect and Mammalian

   Mutation

   delta N, mutant BMP lacking the N terminus (delta…

   Promoter

   CMV

   Available Since

   June 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pUAST-attB-Flag-dTSC2 S1338A

    Plasmid

    #111808

    Purpose

    Insect Expression of dTSC2

    Depositor

    Jun Hee Lee

    Article

    Kim et al Int J Mol Sci. 2015 Mar 27;16(4):7015-26

    Insert

    dTSC2 (TSC2 Fly)
    

    Tags

    3xFlag

    Expression

    Insect

    Mutation

    S1338A

    Available Since

    June 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. WNK3 (WNK3B-c005)

    Plasmid

    #110262

    Purpose

    Baculovirus expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    WNK3 (WNK3 Human)
    

    Tags

    His-TEV

    Expression

    Insect

    Promoter

    polyhedrin promoter

    Available Since

    June 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. WNK3 (WNK3B-c001)

    Plasmid

    #110261

    Purpose

    Baculovirus expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    WNK3 (WNK3 Human)
    

    Tags

    His-TEV

    Expression

    Insect

    Promoter

    polyhedrin promoter

    Available Since

    June 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. PKN2 (PRKCL2A-c038)

    Plasmid

    #110265

    Purpose

    Baculovirus expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    PRKCL2 (PKN2 Human)
    

    Tags

    His6-ZBasic-TEV

    Expression

    Insect

    Promoter

    polyhedrin promoter

    Available Since

    June 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. MTHFR (MTHFRA-c042)

    Plasmid

    #110271

    Purpose

    Baculovirus expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    MTHFR (MTHFR Human)
    

    Tags

    TEV-His10-Flag

    Expression

    Insect

    Promoter

    polyhedrin promoter

    Available Since

    June 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. LIMK2 (LIMK2A-c052)

    Plasmid

    #110264

    Purpose

    Baculovirus expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    LIMK2 (LIMK2 Human)
    

    Tags

    His-TEV

    Expression

    Insect

    Promoter

    polyhedrin promoter

    Available Since

    June 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Hy_pMT dati MCS Exon

    Plasmid

    #110119

    Purpose

    Expression plasmid for expressing circular RNAs of a desired sequence in Drosophila using the dati flanking introns to drive backsplicing

    Depositor

    Jeremy Wilusz

    Article

    Huang et al Genes Dev. 2018 May 17. pii: gad.314856.

    Insert

    dati (dati Fly)
    

    Expression

    Insect

    Promoter

    Metallothionein Promoter (pMT)

    Available Since

    June 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. Hy_pMT dati Exons 1-3

    Plasmid

    #110113

    Purpose

    Drosophila dati minigene plasmid that expresses both a linear RNA and a circular RNA

    Depositor

    Jeremy Wilusz

    Article

    Huang et al Genes Dev. 2018 May 17. pii: gad.314856.

    Insert

    dati (dati Fly)
    

    Expression

    Insect

    Promoter

    Metallothionein Promoter (pMT)

    Available Since

    June 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. Hy_pMT dati MCS circfirefly_1100

    Plasmid

    #110117

    Purpose

    Expresses a 1100-nt firefly luciferase circular RNA in Drosophila cells

    Depositor

    Jeremy Wilusz

    Article

    Huang et al Genes Dev. 2018 May 17. pii: gad.314856.

    Insert

    Firefly luciferase
    

    Expression

    Insect

    Promoter

    Metallothionein Promoter (pMT)

    Available Since

    May 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Hy_pMT dati MCS circfirefly_900

    Plasmid

    #110116

    Purpose

    Expresses a 900-nt firefly luciferase circular RNA in Drosophila cells

    Depositor

    Jeremy Wilusz

    Article

    Huang et al Genes Dev. 2018 May 17. pii: gad.314856.

    Insert

    Firefly luciferase
    

    Expression

    Insect

    Promoter

    Metallothionein Promoter (pMT)

    Available Since

    May 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Hy_pMT dati MCS circfirefly_700

    Plasmid

    #110115

    Purpose

    Expresses a 700-nt firefly luciferase circular RNA in Drosophila cells

    Depositor

    Jeremy Wilusz

    Article

    Huang et al Genes Dev. 2018 May 17. pii: gad.314856.

    Insert

    Firefly luciferase
    

    Expression

    Insect

    Promoter

    Metallothionein Promoter (pMT)

    Available Since

    May 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. Hy_pMT dati MCS circfirefly_500

    Plasmid

    #110114

    Purpose

    Expresses a 500-nt firefly luciferase circular RNA in Drosophila cells

    Depositor

    Jeremy Wilusz

    Article

    Huang et al Genes Dev. 2018 May 17. pii: gad.314856.

    Insert

    Firefly luciferase
    

    Expression

    Insect

    Promoter

    Metallothionein Promoter (pMT)

    Available Since

    May 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pGS_N_Strep

    Plasmid

    #101651

    Purpose

    Expression of type I signal-peptide containing proteins with N-terminal Twin-Strep-tag in insect cells

    Depositor

    Bjoern Sander, Monika Golas

    Article

    Le et al Mol Biotechnol. 2018 Jan;60(1):31-40. do

    Insert

    LRP6 Signal peptide, KpnI linker, Twin-Strep tag
    

    Tags

    Twin-Strep tag

    Expression

    Insect

    Available Since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pGS_C_Strep

    Plasmid

    #101652

    Purpose

    Expression of type I signal-peptide containing proteins with C-terminal Twin-Strep-tag in insect cells

    Depositor

    Bjoern Sander, Monika Golas

    Article

    Le et al Mol Biotechnol. 2018 Jan;60(1):31-40. do

    Insert

    LRP6 Signal peptide, MCS linker, Twin-Strep tag
    

    Tags

    Twin-Strep tag

    Expression

    Insect

    Available Since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pGS_C_3xFLAG

    Plasmid

    #101653

    Purpose

    Expression of type I signal-peptide containing proteins with C-terminal 3xFLAG-tag in insect cells

    Depositor

    Bjoern Sander, Monika Golas

    Article

    Le et al Mol Biotechnol. 2018 Jan;60(1):31-40. do

    Insert

    LRP6 Signal peptide, MCS linker, 3xFLAG tag
    

    Tags

    3xFLAG tag

    Expression

    Insect

    Available Since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pJFRC81-3xVAS1-Syn21-Citrine-HA-P10-3xVAS4-Syn21-FLAG-Cerulean-P10

    Plasmid

    #104619

    Purpose

    Expresses HA tagged Citrine or FLAG tagged Cerulean upon binding of TALE1 to VAS1 or TALE4 to VAS4

    Depositor

    Tudor Fulga

    Article

    Toegel et al Nat Commun. 2017 Nov 21;8(1):1663. doi:

    Insert

    3xVAS4-hsp70-Syn21-FLAG-Cerulean-P10
    

    Tags

    FLAG

    Expression

    Insect

    Promoter

    hsp70

    Available Since

    March 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pJFRC81-3xVAS4-Syn21-FLAG-Cerulean-P10

    Plasmid

    #104618

    Purpose

    Expresses FLAG tagged Cerulean upon binding of TALE4 to VAS4

    Depositor

    Tudor Fulga

    Article

    Toegel et al Nat Commun. 2017 Nov 21;8(1):1663. doi:

    Insert

    hsp70-Syn21-FLAG-mCerulean-P10
    

    Tags

    FLAG

    Expression

    Insect

    Promoter

    hsp70

    Available Since

    March 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pJFRC81-3xVAS4-IVS-Syn21-GFP-P10

    Plasmid

    #104615

    Purpose

    Expresses GFP upon binding of TALE4 to VAS4

    Depositor

    Tudor Fulga

    Article

    Toegel et al Nat Commun. 2017 Nov 21;8(1):1663. doi:

    Insert

    3xVAS4
    

    Expression

    Insect

    Promoter

    hsp70

    Available Since

    Jan. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pJFRC81-3xVAS1-Syn21-Citrine-HA-P10

    Plasmid

    #104616

    Purpose

    Expresses HA tagged Citrine upon binding of TALE1 to VAS1

    Depositor

    Tudor Fulga

    Article

    Toegel et al Nat Commun. 2017 Nov 21;8(1):1663. doi:

    Insert

    Syn21-Citrine-HA-P10-MhcSD
    

    Tags

    HA

    Expression

    Insect

    Promoter

    hsp70

    Available Since

    Jan. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pJC-20xUAS-TALE-VP64-P10

    Plasmid

    #104605

    Purpose

    Control plasmid (no TALE array) with P10 terminator

    Depositor

    Tudor Fulga

    Article

    Toegel et al Nat Commun. 2017 Nov 21;8(1):1663. doi:

    Type

    Empty backbone

    Use

    TALEN

    Expression

    Insect

    Promoter

    hsp70

    Available Since

    Jan. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pAFMW-AthHEN1-CM

    Plasmid

    #104981

    Purpose

    Expression of FLAG Myc tagged catalytic dead A.thaliana-Hen1 (codon optimised for Drosophila) in Drosophila S2 cells

    Depositor

    Stefan Ameres

    Article

    Alberti et al Nat Methods. 2018 Feb 26. pii: nmeth.461

    Insert

    Hen1 (HEN1 Mustard Weed)
    

    Tags

    FLAG Myc

    Expression

    Insect

    Promoter

    Actin5C

    Available Since

    Jan. 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pUAST-Myc-mSesn2 (1186)

    Plasmid

    #66920

    Purpose

    expression of mouse Sesn2 in drosophila

    Depositor

    Jun Hee Lee, Michael Karin

    Article

    Lee et al Science. 2010 Mar 5;327(5970):1223-8. do

    Insert

    Sesn2 (Sesn2 Mouse)
    

    Tags

    Myc

    Expression

    Insect

    Promoter

    HS promoter

    Available Since

    Dec. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pMCh-623

    Plasmid

    #92266

    Purpose

    Plasmid for expression of NHA-tagged Drosophila melanogaster GW182 6W6A with six mutated W-motifs in its N-terminal domain in D. melanogaster cells

    Depositor

    Witold Filipowicz, Marina Chekulaeva

    Article

    Chekulaeva et al Nat Struct Mol Biol. 2011 Oct 7;18(11):1

    Insert

    dGW182 6W6A (gw Fly)
    

    Tags

    NHA

    Expression

    Insect

    Mutation

    The following point mutations were introduced W23…

    Promoter

    Ac5

    Available Since

    Dec. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pMCh-695

    Plasmid

    #92263

    Purpose

    Plasmid for expression of NHA-tagged Drosophila melanogaster GW182 with mutated PAM2 motif in D. melanogaster cells

    Depositor

    Witold Filipowicz, Marina Chekulaeva

    Article

    Chekulaeva et al Nat Struct Mol Biol. 2011 Oct 7;18(11):1

    Insert

    dGW182 PAM2 mut (gw Fly)
    

    Tags

    NHA

    Expression

    Insect

    Mutation

    The following point mutations were introduced: EF…

    Promoter

    Ac5

    Available Since

    Dec. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pMCh-61

    Plasmid

    #92262

    Purpose

    Plasmid for expression of NHA-tagged Drosophila melanogaster GW182 in D. melanogaster cells

    Depositor

    Marina Chekulaeva, Roy Parker

    Article

    Chekulaeva et al RNA. 2009 May;15(5):794-803. doi: 10.126

    Insert

    dGW182 (gw Fly)
    

    Tags

    NHA

    Expression

    Insect

    Promoter

    Ac5

    Available Since

    Dec. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pMCh-76

    Plasmid

    #92288

    Purpose

    Plasmid for expression of NHA-tagged Drosophila melanogaster GW182 CED in D. melanogaster cells

    Depositor

    Marina Chekulaeva, Roy Parker

    Article

    Chekulaeva et al RNA. 2009 May;15(5):794-803. doi: 10.126

    Insert

    dGW182 CED (gw Fly)
    

    Tags

    NHA

    Expression

    Insect

    Mutation

    dGW182 CED: aa 940-1215

    Promoter

    Ac5

    Available Since

    Dec. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pMCh-646

    Plasmid

    #92289

    Purpose

    Plasmid for expression of NHA-tagged Drosophila melanogaster GW182 CED 7W7A with seven mutated W-motifs in D. melanogaster cells

    Depositor

    Witold Filipowicz, Marina Chekulaeva

    Article

    Chekulaeva et al Nat Struct Mol Biol. 2011 Oct 7;18(11):1

    Insert

    dGW182 CED 7W7A (gw Fly)
    

    Tags

    NHA

    Expression

    Insect

    Mutation

    dGW182 CED: aa 940-1215 with the following point …

    Promoter

    Ac5

    Available Since

    Oct. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pMCh-59

    Plasmid

    #92261

    Purpose

    Plasmid for expression of HA-tagged Drosophila melanogaster GW182 CED in D. melanogaster cells

    Depositor

    Marina Chekulaeva, Roy Parker

    Article

    Chekulaeva et al RNA. 2009 May;15(5):794-803. doi: 10.126

    Insert

    dGW182 (gw Fly)
    

    Tags

    HA

    Expression

    Insect

    Mutation

    aa 940-1215

    Promoter

    Ac5

    Available Since

    Oct. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pAc-GFP-dSUMOdeltaC

    Plasmid

    #85697

    Purpose

    expression of GFP-SUMO1 delta C in Drosophila cells

    Depositor

    Licio Collavin, Giannino Del Sal

    Article

    Mauri et al J Biol Chem. 2008 Jul 25. 283(30):20848-

    Insert

    SUMO (Sumo Fly)
    

    Tags

    GFP

    Expression

    Insect

    Mutation

    non-conjugatable SUMO, lacking the C-terminal gly…

    Promoter

    pAc5

    Available Since

    March 21, 2017

    Availability

    Academic Institutions and Nonprofits only

39. pFBD-Btk(PH)Akt1(KD)

    Plasmid

    #86591

    Purpose

    Expresses chimeric fusion of PH domain of Btk and kinase domain of Akt1

    Depositor

    Ivan Yudushkin, Thomas Leonard

    Article

    Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

    Insert

    PH(Btk)-KD(Akt1) fusion (AKT1, BTK Human)
    

    Tags

    10xHis

    Expression

    Insect

    Available Since

    March 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pFBD-Akt13C(WT)

    Plasmid

    #86576

    Purpose

    Expresses cleavable human full-length Akt1(WT)

    Depositor

    Ivan Yudushkin, Thomas Leonard

    Article

    Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

    Insert

    Akt1 (AKT1 Human)
    

    Tags

    10xHis

    Expression

    Insect

    Mutation

    3C cleavage site inserted at pos. 130

    Available Since

    March 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge