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  2. 1073A(HomeR1)=pBac-[Pol-γ35.HL-decPol-γ35-p10]-[U6-gRNA#1(Pol-γ35)]-[3xp3-GFP-SV40]-Pol-γ35.HR-[Opie2-dsRed-SV40]

    Plasmid
    #159676
    Purpose
    Plasmid provides the HomeR#1 gene drive element harboring a rescue, gRNA#1, and 3xP3-eGFP that can be integrated via pBac and inserted at Pol-γ35 site #1 via HDR.
    Depositor
    Omar Akbari
    Article
    Kandul et al Elife. 2021 Mar 5;10. pii: 65939. doi: 1
    Insert
    pBac-[Pol-γ35.HL-decPol-γ35-p10]-[U6-gRNA#1(Pol-γ35)]-[3xp3-GFP-SV40]-Pol-γ35.HR-[Opie2-dsRed-SV40]
    Use
    Tags
    Expression
    Insect
    Mutation
    Promoter
    Available since
    March 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    159676-plasmid-map-sequence-id-314890

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  4. pAcGP67A - murine TfR

    Plasmid
    #12392
    Depositor
    Pamela Bjorkman
    Insert
    transferrin receptor (Tfrc Mouse)
    Use
    Tags
    6X-His tag, Factor Xa cleavage site, and leader p…
    Expression
    Insect
    Mutation
    See comments section
    Promoter
    Available since
    Aug. 17, 2006
    Availability
    Academic Institutions and Nonprofits only
    12392-plasmid-map-sequence-id-274275

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  8. pBig2ab zz TEV YBBR POT1 ZZ TEV TPP1 MBP TEV TIN2 ZZ TEV TRF1 (4comp1)

    Plasmid
    #185447
    Purpose
    Coexpresses human POT1 with a zz affinity tag, YBBR site, and TEV site, human TRF1 and TPP1 each with a zz tag and TEV site, and human TIN2 with an MBP affinity tag and TEV site in insect cells
    Depositor
    Ahmet Yildiz
    Article
    Jack et al Dev Cell. 2022 Jan 24;57(2):277-290.e9.
    Inserts
    TRF1 (TERF1 Human)
    POT1 (POT1 Human)
    TPP1 (TPP1 Human)
    TIN2 (TINF2 Human)
    Use
    Tags
    MBP, YBBR, and ZZ
    Expression
    Insect
    Mutation
    Promoter
    Available since
    June 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    185447-plasmid-map-sequence-id-364982

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  14. pECIA2

    Plasmid
    #47032
    Purpose
    This is a Gateway destination vector for secreted expression in Drosophila cell culture by induction via CuSO4. Inserts will be C-terminally tagged with Fc, V5 and hexahistidine tags.
    Depositor
    Chris Garcia
    Article
    Ozkan et al Cell. 2013 Jul 3;154(1):228-39. doi: 10.
    Type
    Empty backbone
    Use
    Secreted expression in drosophila culture
    Tags
    Fc(human IgG1), HRV 3C Protease Cleavage site, He…
    Expression
    Insect
    Mutation
    Promoter
    Metallothionein (Copper-Inducible)
    Available since
    Aug. 30, 2013
    Availability
    Academic Institutions and Nonprofits only
    47032-plasmid-map-sequence-id-67069

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  16. pTriEx-mCherry-PA-Rac1-T17N

    Plasmid
    #22029
    Purpose
    Expression of photoactivatable Rac1 (dominant-negative mutant).
    Depositor
    Klaus Hahn
    Article
    Wu et al Nature. 2009 Sep 3. 461(7260):104-8.
    Insert
    PA-Rac1-T17N (RAC1 Human, Avena sativa (oat))
    Use
    Tags
    6xHis and mCherry
    Expression
    Bacterial, Insect, and Mamm…
    Mutation
    LOV(Leu404-Leu546). Rac1 starts at Ile4 and conta…
    Promoter
    CMV, p10
    Available since
    Sept. 29, 2009
    Availability
    Academic Institutions and Nonprofits only
    22029-plasmid-map-sequence-id-239317

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  17. RABGEF1A-c014

    Plasmid
    #175478
    Purpose
    Protein expression in insect cells. Entire CDS, M1-G491. Codon optimised.
    Depositor
    Opher Gileadi
    Insert
    RABGEF1 (RABGEF1 Human)
    Use
    Tags
    His6-Zbasic-TEV
    Expression
    Insect
    Mutation
    Promoter
    Available since
    Jan. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    175478-plasmid-map-sequence-id-353251

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  22. pACEBac1-CoRESTfl

    Plasmid
    #109156
    Purpose
    Encodes human full-length CoREST with C-terminal 3xFLAG tag to be expressed in a baculovirus/insect cell expression system
    Depositor
    Monika Golas
    Article
    Inui et al Protein Sci. 2017 May;26(5):997-1011. do
    Insert
    human full-length CoREST, sequence-optimized for insect cells (RCOR1 Human)
    Use
    Tags
    3xFLAG
    Expression
    Insect
    Mutation
    Promoter
    Polyhedrin
    Available since
    Jan. 11, 2019
    Availability
    Academic Institutions and Nonprofits only
    109156-plasmid-map-sequence-id-232884

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Showing: 451 - 480 of 4828 results