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Showing: 691 - 720 of 4828 results

  2. ANO10 (TMEM16KA-c001)

    Plasmid
    #110267
    Purpose
    Baculovirus expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    TMEM16K (ANO10 Human)
    Use
    Tags
    TEV-His10-Flag
    Expression
    Insect
    Mutation
    Promoter
    polyhedrin promoter
    Available since
    April 25, 2019
    Availability
    Academic Institutions and Nonprofits only
    110267-plasmid-map-sequence-id-358733

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  4. pGP-20XUAS-IVS-Syn21-op1-jGCaMP7f-T2A-tdTomato-p10

    Plasmid
    #172915
    Purpose
    Gal4 activated transgene coexpression of protein calcium sensor and tdTomato under the hsp70 promoter in D. melanogaster
    Depositor
    GENIE Project
    Insert
    jGCaMP7f-T2A-tdTomato
    Use
    Tags
    6xHis and T2A-tdTomato
    Expression
    Insect
    Mutation
    Promoter
    hsp70
    Available since
    Aug. 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    172915-plasmid-map-sequence-id-341221

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  7. pOCC102-MBP-eIF4G

    Plasmid
    #219971
    Purpose
    eIF4G protein purification
    Depositor
    Simon Alberti
    Insert
    eIF4G (TIF4631 Budding Yeast)
    Use
    Tags
    6xHis-MBP
    Expression
    Insect
    Mutation
    Promoter
    Available since
    June 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    219971-plasmid-map-sequence-id-436844

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  8. pMT-TAK1-V5-His

    Plasmid
    #63753
    Purpose
    Expresses drosophila TAK1 tagged with V5 and 6xHis epitopes, under control of metallothionein promoter
    Depositor
    Cyril Gueydan
    Insert
    TGF-β activated kinase 1 (Tak1 Fly)
    Use
    Tags
    V5-His
    Expression
    Insect
    Mutation
    Promoter
    pMT
    Available since
    April 6, 2015
    Availability
    Academic Institutions and Nonprofits only
    63753-plasmid-map-sequence-id-106438

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  9. PRE-Hsp70BbCas9_1.2

    Plasmid
    #190796
    Purpose
    gypsyPRE-Hsp70Bb-Cas9-T2A-eGFP; heat-shock inducible Cas9-T2A-eGFP expression regulated by upstream Polycomb response elements with one gypsy insulator element. Step 2 in cloning process.
    Depositor
    Omar Akbari
    Article
    Warsinger-Pepe et al G3 (Bethesda). 2023 Apr 11;13(4):jkad024
    Insert
    gypsyPRE-Hsp70Bb-Cas9-T2A-eGFP
    Use
    Tags
    Expression
    Insect
    Mutation
    Promoter
    Available since
    Nov. 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    190796-plasmid-map-sequence-id-376571

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  11. pOCC65

    Plasmid
    #118865
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C protease, and N-terminal monomeric GFP
    Depositor
    PEPC MPI CBG, Marc Bickle
    Article
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-HIS6-3C-mGFP-NotI-ccdB-AscI-stop-HindIII cassette
    Use
    Tags
    HIS6, cleavable with 3C protease, mGFP
    Expression
    Insect
    Mutation
    Promoter
    polH
    Available since
    Jan. 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    118865-plasmid-map-sequence-id-232317

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  12. pACEBac1-PARP1-Flag-His-7xKQ

    Plasmid
    #111573
    Purpose
    Expresses full length PARP1 with K to Q mutations in automodification domain in Sf9 (insect) cells
    Depositor
    Thomas Muir
    Article
    Liszczak et al Nat Chem Biol. 2018 Jul 16. pii: 10.1038
    Insert
    PARP1 (PARP1 Human)
    Use
    Tags
    C-terminal 6xHis and C-terminal Flag
    Expression
    Insect
    Mutation
    lysine to glutamine mutation at the following ami…
    Promoter
    polyhedrin promoter
    Available since
    July 18, 2018
    Availability
    Academic Institutions and Nonprofits only
    111573-plasmid-map-sequence-id-215588

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  15. pKSB-618 U6-sgRNA2/3 GGAA AACA

    Plasmid
    #173674
    Purpose
    To clone and express a gRNA in Anopheles
    Depositor
    Eric Marois
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Insect
    Mutation
    Promoter
    Available since
    Sept. 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    173674-plasmid-map-sequence-id-342962

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  16. pOCC154

    Plasmid
    #118902
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with C-terminal TwinStrep, cleavable with 3C
    Depositor
    PEPC MPI CBG, Marc Bickle
    Article
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-NotI-ccdB-AscI-3C-TwinStrep-stop-HindIII cassette
    Use
    Tags
    TwinStrep, cleavable with 3C protease
    Expression
    Insect
    Mutation
    Promoter
    polH
    Available since
    Jan. 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    118902-plasmid-map-sequence-id-232325

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  18. pAc5.1C-RLuc-A90-HhR_O

    Plasmid
    #147073
    Purpose
    Insect Expression of A90-HhR
    Depositor
    Elisa Izaurralde
    Insert
    A90-HhR
    Use
    Tags
    Expression
    Insect
    Mutation
    A127 instead of A90 (see comments below)
    Promoter
    Available since
    Jan. 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    147073-plasmid-map-sequence-id-456973

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  23. pCoHygro L16_shRNA of spliced Copia

    Plasmid
    #71389
    Purpose
    knock down expression of spliced Copia in Drosophila melanogaster
    Depositor
    Timothy Nilsen
    Insert
    Copia
    Use
    Tags
    Expression
    Insect
    Mutation
    Promoter
    U6
    Available since
    Nov. 16, 2015
    Availability
    Academic Institutions and Nonprofits only
    71389-plasmid-map-sequence-id-129772

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  26. pWALIUM10-GW-TurboID-V5

    Plasmid
    #196283
    Purpose
    Gateway destination vector to express C-terminal TurboID fusion under UAS promoter, for making transgenic flies
    Depositor
    Norbert Perrimon
    Type
    Empty backbone
    Use
    Gateway destination vector
    Tags
    Expression
    Insect
    Mutation
    Promoter
    Available since
    March 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    196283-plasmid-map-sequence-id-385988

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  29. pQUASp-RedStinger (nlsRFP)

    Plasmid
    #46165
    Purpose
    Expression of RedStinger in insect
    Depositor
    Christopher Potter
    Type
    Empty backbone
    Use
    Tags
    Expression
    Insect
    Mutation
    Promoter
    Available since
    Aug. 7, 2013
    Availability
    Academic Institutions and Nonprofits only
    46165-plasmid-map-sequence-id-65049

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Showing: 691 - 720 of 4828 results