5,013 results

Showing: 691 - 720 of 5013 results

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pAc V5 dDispatched WT HA

Plasmid

#126405

Purpose

Expression of V5 dDispatched WT HA in Drosophila cells lines.

Depositor

Stacey Ogden

Article

Stewart et al Elife. 2018 Jan 23;7. pii: 31678. doi: 1

Insert

d-Dispatched1 (disp Fly)

Tags

HA

Expression

Insect

Available Since

June 13, 2019

Availability

Academic Institutions and Nonprofits only

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pUb RxCas13d + RxCas13d guide RNA

Plasmid

#176303

Purpose

Expresses RxCas13d and its associated guide RNA in Drosophila cells

Depositor

Jeremy Wilusz

Article

Ai et al Nucleic Acids Res. 2022 Mar 4. pii: 6542

Insert

RxCas13d

Tags

HA tag

Expression

Insect

Promoter

Ubi-p63e

Available Since

March 7, 2022

Availability

Academic Institutions and Nonprofits only

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pFastBac_HT_B_6xHis-TEV-GFP-NBR1

Plasmid

#190928

Purpose

Plasmid for the expression and purification of GFP labelled NBR1. Internal reference: SMC 914

Depositor

Sascha Martens

Article

Turco et al Nat Commun. 2021 Sep 1;12(1):5212. doi:

Insert

NBR1 (NBR1 Human)

Tags

Strep-TEV-GFP

Expression

Insect

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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47 pLIB_GST

Plasmid

#160272

Purpose

N-term 3C cleavable GST tag for insect cell expression

Depositor

John Weir

Article

Altmannova et al Protein Sci. 2020 Sep 21. doi: 10.1002/p

Type

Empty backbone

Tags

GST

Expression

Insect

Promoter

polyhydrin

Available Since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pFB-LIC-Bse

Plasmid

#26108

Purpose

SGC Baculovirus transfer vector with His tag in 22-aa N-terminal fusion peptide with TEV protease cleavage site. Includes sites for LIC cloning, and SacB gene for negative selection

Depositor

Opher Gileadi

Article

Savitsky et al J Struct Biol. 2010 Oct;172(1):3-13. doi

Type

Empty backbone

Use

Baculovirus expression

Tags

His6 - TEV

Expression

Insect

Promoter

polyhedrin

Available Since

Oct. 4, 2010

Availability

Academic Institutions and Nonprofits only

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RhoC FLARE.sc mCer, mVenus - wt

Plasmid

#65071

Purpose

RhoC biosensor single chain; mCerulean-tagged ROCK-RBD, mVenus-tagged RhoC

Depositor

Klaus Hahn

Article

Zawistowski et al PLoS One. 2013 Nov 5;8(11):e79877. doi:

Insert

RBD, mCerulean, mVenus, RhoC

Expression

Bacterial, Insect, and Mamm…

Mutation

T153M in mVenus

Promoter

CMV, p10

Available Since

July 9, 2015

Availability

Academic Institutions and Nonprofits only
pUASp YFP Ras85D CA

Plasmid

#52247

Purpose

Drosophila expression of YFP-Ras85D constitutively active mutant

Depositor

Matthew Scott

Insert

Ras85D (Ras85D Fly)

Use

Drosophila

Tags

YFP

Expression

Insect

Mutation

Q61L; CA mutation

Promoter

GAL4

Available Since

March 21, 2014

Availability

Academic Institutions and Nonprofits only
pFastbac1-GST-hUSP7

Plasmid

#63573

Purpose

Expression of synthetic human USP7 tagged with GST

Depositor

Titia Sixma

Article

Faesen et al Mol Cell. 2011 Oct 7;44(1):147-59. doi:

Insert

USP7 (USP7 Human, Synthetic)

Tags

GST

Expression

Insect

Mutation

Synthetic based on human protein sequence (please…

Promoter

Polyhedrin

Available Since

April 17, 2015

Availability

Academic Institutions and Nonprofits only

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pFastBac1 PIK3R1

Plasmid

#17743

Depositor

Bert Vogelstein

Article

Samuels et al Cancer Cell. 2005 Jun . 7(6):561-73.

Insert

PIK3R1 (PIK3R1 Human)

Expression

Insect

Available Since

April 14, 2008

Availability

Academic Institutions and Nonprofits only
GFP-Rab32

Plasmid

#31744

Depositor

Ron Vale

Article

Huckaba et al J Biol Chem. 2011 Mar 4;286(9):7457-67.

Insert

Rab32 (Rab32 Fly)

Tags

GFP

Expression

Insect

Promoter

Actin

Available Since

Sept. 21, 2011

Availability

Academic Institutions and Nonprofits only
pAct:dCas9-GCN4

Plasmid

#78899

Purpose

Expresses dCas9-GCN4 under actin promoter for SunTag CRISPRa in Drosophila cell culture

Depositor

Norbert Perrimon

Article

Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

Insert

dCas9-10XGCN4

Use

CRISPR

Tags

HA

Expression

Insect

Promoter

pActin (Drosophila)

Available Since

Aug. 12, 2016

Availability

Academic Institutions and Nonprofits only

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dVGlut-Gal80

Plasmid

#52555

Purpose

Expresses Gal80 under vesicular glutamate transporter promoter

Depositor

Leslie Vosshall

Insert

VGlut-Gal80 (VGlut, GAL80 Fly, Budding Yeast)

Expression

Insect

Promoter

Drosophila Synthetic Core Promoter

Available Since

June 2, 2014

Availability

Academic Institutions and Nonprofits only

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UAS-u(M)Cas9

Plasmid

#127384

Purpose

Expresses Cas9 at medium level

Depositor

Michael Boutros

Article

Port et al Elife. 2020 Feb 13;9. pii: 53865. doi: 1

Insert

uORF(M)-Cas9

Use

CRISPR

Expression

Insect

Available Since

Jan. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pGP-JFRC59-13XLexAop2-IVS-Syn21-jGCaMP8m-p10

Plasmid

#162384

Purpose

LexA activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogaster

Depositor

GENIE Project

Article

Zhang et al Janelia Research Campus, Online Resource

Insert

jGCaMP8m

Tags

6xHis

Expression

Insect

Mutation

A25G F286Y

Promoter

hsp70

Available Since

May 3, 2021

Availability

Academic Institutions and Nonprofits only

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pB[nos-Cas9 PUb-ZsGreen]

Plasmid

#169011

Purpose

For germline transformation. Expresses Cas9 in germ cells and ZsGreen marker in all cells.

Depositor

Max Scott

Article

Kandul et al CRISPR J. 2021 Oct;4(5):739-751. doi: 10

Insert

Cas9

Use

Piggybac

Expression

Insect

Promoter

Drosophila melanogaster nanos

Available Since

April 11, 2022

Availability

Academic Institutions and Nonprofits only

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pFB1.HMBP.PrS.AEBP2

Plasmid

#125165

Purpose

Expresses human AEBP2 in insect cells, , under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.

Depositor

Chen Davidovich

Article

Zhang et al Nat Struct Mol Biol. 2019 Mar;26(3):237-

Insert

Zinc finger protein AEBP2 (AEBP2 Human)

Tags

His-MBP

Expression

Insect

Promoter

Polyhedrin

Available Since

April 27, 2020

Availability

Academic Institutions and Nonprofits only

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pJFRC165-20XUAS-IVS-R::PEST

Plasmid

#32142

Depositor

Gerald Rubin

Article

Nern et al Proc Natl Acad Sci U S A. 2011 Aug 9.

Insert

R::PEST

Expression

Insect

Available Since

Sept. 23, 2011

Availability

Academic Institutions and Nonprofits only

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pFB-EPCP5

Plasmid

#228879

Purpose

Expression of Z-tagged LRRK2 KW using Baculoviruses

Depositor

Sebastian Mathea

Article

Raig et al bioRxiv [Preprint]. 2025 Feb 13:2024.09.

Insert

Leucine-rich repeat kinase 2 (LRRK2 Human)

Tags

His-Z-TEV

Expression

Insect

Mutation

Amino acids deleted 1-1846

Available Since

Jan. 28, 2025

Availability

Academic Institutions and Nonprofits only

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PUC19_C_tag

Plasmid

#186659

Purpose

Empty donor plasmid for C-terminal tag of 3xFlag-3xHA.

Depositor

Astrid Haase

Article

Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

Insert

C-terminal 3xFlag-3xHA Tag

Tags

3xFlag-3xHA

Expression

Insect

Available Since

July 21, 2022

Availability

Academic Institutions and Nonprofits only

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pETDuet-1_6xHis-TEV-mCh-NDP52

Plasmid

#187829

Purpose

Plasmid for the expression and purification of 6xHis-TEV-mCh-NDP52 in E. coli Rosetta pLysS. Internal Reference: SMC1148

Depositor

Sascha Martens

Article

Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

Insert

NDP52 (CALCOCO2 Human)

Tags

6xHisTag, TEV, and mCherry

Expression

Bacterial, Insect, and Mamm…

Available Since

Oct. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pUASTLOTattB_mCherry::T48-Baz::vhhGFP4

Plasmid

#163928

Purpose

Expression of GrabFP-A-intracellular under control of UAS or LOP enhancer

Depositor

Markus Affolter

Article

Harmansa et al Elife. 2017 Apr 11;6. doi: 10.7554/eLife

Insert

Fusion of vhhGPF4 (intracellular) nanobody with T48 protein, Bazooka minimal localization sequence and mCherry fluorophor

Use

Cre/Lox

Tags

mCherry

Expression

Insect

Available Since

Jan. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pJFRC4-3XUAS-IVS-mCD8::GFP

Plasmid

#26217

Depositor

Gerald Rubin

Article

Pfeiffer et al Genetics. 2010 Oct;186(2):735-55. doi: 1

Insert

mCD8::GFP

Expression

Insect

Available Since

Oct. 19, 2010

Availability

Academic Institutions and Nonprofits only

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pSLfa-PUb-MCS

Plasmid

#52908

Purpose

Aedes aegypti polyubiquitin promoter followed by MCS

Depositor

Zach Adelman

Type

Empty backbone

Expression

Insect

Promoter

Ae aegptyi polyubiquitin

Available Since

June 11, 2014

Availability

Academic Institutions and Nonprofits only

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OA-986B

Plasmid

#124999

Purpose

Expresses dCas9-VPR driven by the Ubiquitin-63E promoter

Depositor

Omar Akbari

Article

Buchman et al Nat Commun. 2021 Jun 2;12(1):3281. doi:

Insert

dCas9-VPR

Use

Piggyback

Expression

Insect

Promoter

Ubiquitin

Available Since

Oct. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pIHEU-FlipGFP(Casp3 cleavage seq) T2A mCherry

Plasmid

#124434

Purpose

Expresses FlipGFP (caspase-3 cleavage sequence) and T2A mCherry in Drosophila

Depositor

Xiaokun Shu

Article

Zhang et al J Am Chem Soc. 2019 Mar 1. doi: 10.1021/

Insert

FlipGFP(Casp3 cleavage seq) T2A mCherry

Expression

Insect

Available Since

April 18, 2019

Availability

Academic Institutions and Nonprofits only

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pJFRC208-10XUAS-FRT>STOP>FRT-myr::smGFP-FLAG

Plasmid

#63169

Purpose

GAL4-responsive UAS “flp-On” spaghetti monster GFP-FLAG reporter

Depositor

Gerald Rubin

Article

Nern et al Proc Natl Acad Sci U S A. 2015 May 11. p

Insert

"spaghetti monster GFP-FLAG"

Tags

N-myristoylation

Expression

Insect

Available Since

May 18, 2015

Availability

Academic Institutions and Nonprofits only

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pOPINN-GFP

Plasmid

#53541

Purpose

pTirex based GFP fusion vector for expression in mammalian, E. coli and insect cells using the baculovirus system

Depositor

Ray Owens

Insert

GFP

Tags

N-His-GFP

Expression

Bacterial, Insect, and Mamm…

Available Since

June 17, 2014

Availability

Academic Institutions and Nonprofits only

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Strep-His-Tev-hCGN

Plasmid

#236512

Purpose

For expression of Cingulin (human) in insect cells

Depositor

Sandra Citi

Article

Rouaud et al J Cell Biol. 2023 Jul 3;222(7):e20220806

Insert

CGN (CGN Human)

Tags

2xStrep-10xHis-Tev

Expression

Insect

Mutation

WT

Available Since

May 1, 2025

Availability

Academic Institutions and Nonprofits only

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pAW-CD8-Nb127D01-GFP

Plasmid

#171577

Purpose

Expresses GFP-tagged anti-CXCR2 (human) recombinant llama nanobody (Nb127D01-GFP) in fly cell cytomembrane

Depositor

Norbert Perrimon, Hidde Ploegh

Article

Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:

Insert

CD8-Nb127D01-GFP (CXCR2 Human)

Tags

CD8 and GFP

Expression

Insect

Promoter

fly actin5C promoter

Available Since

June 30, 2021

Availability

Academic Institutions and Nonprofits only

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pBS-KS-attB1-2-PT-SA-SD-1-GFP11

Plasmid

#172068

Purpose

Protein trap plasmid for inserting GFP11 into a phase 1 coding intron

Depositor

Daichi Kamiyama

Article

Kamiyama et al Proc Natl Acad Sci U S A. 2021 Jun 8;118

Insert

GFP11x7

Expression

Insect

Available Since

Oct. 20, 2021

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge

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5,013 results