4,908 results

1. pCMycDam

   Plasmid

   #59218

   Purpose

   Drosophila DamID vector used to fuse gene of interest to Myc-tagged E.coli Dam

   Depositor

   Bas van Steensel

   Article

   van Steensel et al Nat Biotechnol. 2000 Apr;18(4):424-8.

   Type

   Empty backbone

   Use

   Damid

   Tags

   Dam and Myc

   Expression

   Insect

   Mutation

   Promoter

   Drosophila heat-shock promoter

   Available since

   May 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAC-GAL80

   Plasmid

   #24346

   Depositor

   Liqun Luo

   Article

   Potter et al Cell. 2010 Apr 30. 141(3):536-48.

   Insert

   GAL80 (GAL80 )
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   March 31, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAct:dCas9

   Plasmid

   #78903

   Purpose

   Expresses dCas9 under actin promoter for "Scaffold" CRISPRa in Drosophila cells

   Depositor

   Norbert Perrimon

   Article

   Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

   Insert

   dCas9
   

   Use

   CRISPR

   Tags

   Expression

   Insect

   Mutation

   Promoter

   pActin (Drosophila)

   Available since

   Aug. 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBPGw

   Plasmid

   #17574

   Depositor

   Gerald Rubin

   Article

   Pfeiffer et al Tools for neuroanatomy and neurogenetics

   Insert

   GAL4 (GAL4 Budding Yeast)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   March 28, 2008

   Availability

   Academic Institutions and Nonprofits only

5. U6c

   Plasmid

   #117223

   Purpose

   Expresses gRNA in Aedes aegypti

   Depositor

   Omar Akbari

   Article

   Li et al Elife. 2020 Jan 21;9. pii: 51701. doi: 1

   Insert

   HR U6c promoter-gRNA
   

   Use

   Synthetic Biology

   Tags

   Expression

   Insect

   Mutation

   Promoter

   U6

   Available since

   Jan. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBig2ab zz TEV YBBR POT1 ZZ TEV TPP1 MBP TEV TIN2 ZZ TEV TRF1 (4comp1)

   Plasmid

   #185447

   Purpose

   Coexpresses human POT1 with a zz affinity tag, YBBR site, and TEV site, human TRF1 and TPP1 each with a zz tag and TEV site, and human TIN2 with an MBP affinity tag and TEV site in insect cells

   Depositor

   Ahmet Yildiz

   Article

   Jack et al Dev Cell. 2022 Jan 24;57(2):277-290.e9.

   Inserts

   TRF1 (TERF1 Human)
   POT1 (POT1 Human)
   TPP1 (TPP1 Human)
   TIN2 (TINF2 Human)
   

   Use

   Tags

   MBP, YBBR, and ZZ

   Expression

   Insect

   Mutation

   Promoter

   Available since

   June 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAWG-dCas9-VPR

   Plasmid

   #63802

   Purpose

   SP-dCas9 with VP64-p65-Rta (VPR) fused to it's C-terminus; drosophila vector

   Depositor

   George Church

   Article

   Chavez et al Nat Methods. 2015 Mar 2. doi: 10.1038/nm

   Insert

   SP-dCas9-VPR
   

   Use

   CRISPR and Synthetic Biology

   Tags

   VPR (VP64-p65-Rta)

   Expression

   Insect

   Mutation

   Promoter

   act5c

   Available since

   May 14, 2015

   Availability

   Academic Institutions and Nonprofits only

8. pKF295

   Plasmid

   #72871

   Purpose

   expression of improved FLP recombinase

   Depositor

   Klaus Foerstemann

   Article

   Kunzelmann et al G3 (Bethesda). 2016 Jun 1;6(6):1777-85.

   Insert

   FLP recombinase (flp Budding Yeast)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   P2->S, added NLS

   Promoter

   Available since

   March 9, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFB1.HMBP.PrS.EED

   Plasmid

   #125163

   Purpose

   Expresses human EED in insect cells, , under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.

   Depositor

   Chen Davidovich

   Article

   Zhang et al Nat Struct Mol Biol. 2019 Mar;26(3):237-

   Insert

   Polycomb protein EED (EED Human)
   

   Use

   Tags

   His-MBP

   Expression

   Insect

   Mutation

   Promoter

   Polyhedrin

   Available since

   April 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pUWR-Cad-Venus

    Plasmid

    #58328

    Purpose

    Destination vector for inserting ubi-Cad-Venus to Drosophila melanogaster genome

    Depositor

    Denise Montell

    Article

    Cai et al Cell. 2014 May 22;157(5):1146-59. doi: 1

    Insert

    Cad-Venus
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    poly-ubiquitin

    Available since

    Aug. 14, 2014

    Availability

    Academic Institutions and Nonprofits only

11. pDK521

    Plasmid

    #134204

    Purpose

    Baculovirus expresion of Astrin, LC8, and SKAP

    Depositor

    Iain Cheeseman

    Article

    Kern et al Elife. 2017 Aug 25;6. pii: 26866. doi: 1

    Insert

    Astrin 1-693-sGFP-His BroAstrin and LC8 and AltSKAP in pACE1
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Feb. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pOPINMSYB

    Plasmid

    #41118

    Depositor

    Ray Owens

    Article

    Bird et al Methods. 2011 Sep;55(1):29-37. Epub 2011

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    Promoter

    Available since

    March 27, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. pUb dPspCas13b + PspCas13b guide RNA

    Plasmid

    #176306

    Purpose

    Expresses catalytic dead PspCas13b and its associated guide RNA in Drosophila cells

    Depositor

    Jeremy Wilusz

    Article

    Ai et al Nucleic Acids Res. 2022 Mar 4. pii: 6542

    Insert

    PspCas13b
    

    Use

    Tags

    HA tag

    Expression

    Insect

    Mutation

    H133A, H1058A

    Promoter

    Ubi-p63e

    Available since

    March 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDropIn-T2A-split-intein-Gal4[1-20]-N-int-BlastR

    Plasmid

    #199197

    Purpose

    Insert to create split-intein Gal4[N-int] knock-in vectors, with blasticidin resistance, via Drop-In cloning, released via BsmBI digest

    Depositor

    Norbert Perrimon

    Article

    Ewen-Campen et al Proc Natl Acad Sci U S A. 2023 Jun 13;12

    Insert

    T2A-split-intein-Gal4[1-20]-N-int-BlastR
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Aug. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDropIn-T2A-split-intein-Gal4[21-881]-C-int-G418R

    Plasmid

    #199198

    Purpose

    Insert to create split-intein Gal4[21-881]-C-int knock-in vectors, with G418 resistance, via Drop-In cloning, released via BsmBI digest

    Depositor

    Norbert Perrimon

    Article

    Ewen-Campen et al Proc Natl Acad Sci U S A. 2023 Jun 13;12

    Insert

    T2A-split-intein-Gal4[21-881]-C-int-G418R
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Aug. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pFB1.HMBP.PrS.dEZH2

    Plasmid

    #220241

    Purpose

    Expression of an EZH2 catalytic site mutant in insect cells.

    Depositor

    Chen Davidovich

    Article

    Gail et al Nat Genet. 2024 May 14. doi: 10.1038/s41

    Insert

    dEZH2 (EZH2 Human)
    

    Use

    Tags

    6x His, MBP

    Expression

    Insect

    Mutation

    Y731F

    Promoter

    Polyhedrin

    Available since

    June 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pFastBac1 Flag BAF155 b

    Plasmid

    #1954

    Depositor

    Robert Kingston

    Article

    Phelan et al Mol Cell 1999 Feb;3(2):247-53.

    Insert

    BAF155 (SMARCC1 Human)
    

    Use

    Tags

    flag

    Expression

    Insect

    Mutation

    Promoter

    Available since

    May 24, 2006

    Availability

    Academic Institutions and Nonprofits only

18. pFB1.HMBP.PrS.JARID2

    Plasmid

    #125166

    Purpose

    Expresses human JARID2 in insect cells, , under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.

    Depositor

    Chen Davidovich

    Article

    Zhang et al Nat Struct Mol Biol. 2019 Mar;26(3):237-

    Insert

    JARID2 (JARID2 Human)
    

    Use

    Tags

    His-MBP

    Expression

    Insect

    Mutation

    Promoter

    Polyhedrin

    Available since

    April 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pJFRC14-10XUAS-IVS-GFP-WPRE

    Plasmid

    #26223

    Depositor

    Gerald Rubin

    Article

    Pfeiffer et al Genetics. 2010 Oct;186(2):735-55. doi: 1

    Insert

    GFP
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Oct. 19, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCFD3-frame_selector_0

    Plasmid

    #127553

    Purpose

    Drosophila expression of frame selector sgRNA 0 that targets CRISPaint site for homology-independent knock-in

    Depositor

    Norbert Perrimon

    Article

    Bosch et al Genetics. 2019 Nov 4. pii: genetics.119.

    Insert

    sgRNA frame 0
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    dU6:3

    Available since

    July 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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