5,017 results

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pSK23

Plasmid

#72851

Purpose

template for C-terminal eGFP tagging, Puromycin-resistance

Depositor

Klaus Foerstemann

Article

Kunzelmann et al G3 (Bethesda). 2016 Jun 1;6(6):1777-85.

Insert

eGFP

Expression

Insect

Promoter

copia

Available Since

March 9, 2016

Availability

Academic Institutions and Nonprofits only

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pFB1.HMBP.PrS.EPOP

Plasmid

#125170

Purpose

Expresses human EPOP in insect cells, under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.

Depositor

Chen Davidovich

Article

Zhang et al Nat Struct Mol Biol. 2019 Mar;26(3):237-

Insert

EPOP (EPOP Human)

Tags

His-MBP

Expression

Insect

Promoter

Polyhedrin

Available Since

May 7, 2020

Availability

Academic Institutions and Nonprofits only

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pTriex-mVenus-PI-RhoA(Q63L)

Plasmid

#86978

Purpose

PI(DM): Photo-activatable/light sensitive

Depositor

Klaus Hahn

Article

Dagliyan et al Science. 2016 Dec 16;354(6318):1441-1444

Inserts

LOV2
RhoA (RHOA Human)

Tags

6xHis and mVenus

Expression

Bacterial, Insect, and Mamm…

Mutation

Q63L

Promoter

CMV

Available Since

Feb. 24, 2017

Availability

Academic Institutions and Nonprofits only

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pTriEX-Antenna-GDI Cdc42

Plasmid

#101681

Purpose

"Cdc42 was modified with a FRET ‘binding antenna’ that selectively reported Cdc42 binding to endogenous GDI..." -Hodgson et al

Depositor

Klaus Hahn

Article

Hodgson et al Nat Chem Biol. 2016 Aug 8. doi: 10.1038/

Insert

Cdc42

Tags

6xHis and FRET Antenna (mCerulean-cpVenus)

Expression

Bacterial, Insect, and Mamm…

Promoter

CMV

Available Since

March 23, 2018

Availability

Academic Institutions and Nonprofits only

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pAct:dCas9-VP64

Plasmid

#78901

Purpose

Expresses dCas9-VP64 under actin promoter for SAM CRISPRa in Drosophila cells

Depositor

Norbert Perrimon

Article

Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

Insert

dCas9-VP64

Use

CRISPR

Expression

Insect

Promoter

pActin (Drosophila)

Available Since

Oct. 11, 2016

Availability

Academic Institutions and Nonprofits only

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U6c-T

Plasmid

#117211

Purpose

Expresses gRNA in Aedes aegypti

Depositor

Omar Akbari

Article

Li et al Elife. 2020 Jan 21;9. pii: 51701. doi: 1

Insert

u6c promoter-gRNA

Use

Synthetic Biology

Expression

Insect

Promoter

U6

Available Since

Jan. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pScarlessHD-N-3x127D01-DsRed

Plasmid

#171579

Purpose

For scarless insertion of 3x127D01 NanoTag into N-term; Marked by the visible marker 3xP3-DsRed flanked by piggyBac termini.

Depositor

Norbert Perrimon, Hidde Ploegh

Article

Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:

Insert

3x127D01 (CXCR2 Human)

Expression

Insect

Available Since

June 30, 2021

Availability

Academic Institutions and Nonprofits only

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pBFv-UAS3-GFP1-10

Plasmid

#138398

Purpose

Expresses split GFP fragment (GFP1-10)

Depositor

Shu Kondo

Article

Kondo et al Cell Rep. 2020 Jan 7;30(1):284-297.e5. d

Insert

GFP1-10

Expression

Insect

Available Since

March 19, 2020

Availability

Academic Institutions and Nonprofits only

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pTriEx-mVenus-Zdk1-RhoA Q63L

Plasmid

#81046

Purpose

mammalian expression of Zdk1 tagged RhoA Q63L

Depositor

Klaus Hahn

Article

Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

Inserts

Zdk1
RhoA Q63L (RHOA Human)

Tags

mVenus

Expression

Bacterial, Insect, and Mamm…

Available Since

Aug. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pTriEx-mCherry-Zdk1-Rac1 Q61L

Plasmid

#81059

Purpose

mammalian expression of Zdk1 tagged Rac1 Q61L

Depositor

Klaus Hahn

Article

Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

Inserts

Zdk1
Rac1 Q61L (RHOA Human)

Tags

mCherry

Expression

Bacterial, Insect, and Mamm…

Available Since

Aug. 30, 2016

Availability

Academic Institutions and Nonprofits only
pCaryP

Plasmid

#18945

Depositor

Michele Calos

Article

Groth et al Genetics. 2004 Apr . 166(4):1775-82.

Insert

phiC31 attP

Expression

Insect

Available Since

Oct. 27, 2008

Availability

Academic Institutions and Nonprofits only

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VanGlow-GL

Plasmid

#83342

Purpose

for Gateway cloning of promoter elements upstream of a GFP::Luciferase(nls) reporter, facilitating qualitative and quantitative analysis of expression in transgenic fly lines and S2 cells.

Depositor

Cheng-Yu Lee

Article

Janssens et al Dev Cell. 2017 Feb 27;40(4):367-380.e7.

Type

Empty backbone

Use

Luciferase

Tags

GFP::Luciferase(nls)

Expression

Insect

Available Since

Dec. 2, 2016

Availability

Academic Institutions and Nonprofits only

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pQUAST-mtdT-3XHA

Plasmid

#24354

Depositor

Liqun Luo

Article

Potter et al Cell. 2010 Apr 30. 141(3):536-48.

Insert

mtDT-3XHA

Expression

Insect

Available Since

April 30, 2010

Availability

Academic Institutions and Nonprofits only

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10XUAS-flp-DD

Plasmid

#124777

Purpose

Expresses flippase fused with destabilizing domain (DD) downstream of a 10XUAS sequence.

Depositor

Jing-W Wang

Article

Sethi et al Elife. 2017 Nov 15;6. pii: e30327. doi:

Insert

TMP-inducible destabilized FLP (FLP-DD)

Tags

DD

Expression

Insect

Available Since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

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pAW-CD8-NbVHH05-GFP

Plasmid

#171576

Purpose

Expresses GFP-tagged anti-UBC6e (human) recombinant alpaca nanobody (NbVHH05-GFP) in fly cell cytomembrane

Depositor

Norbert Perrimon, Hidde Ploegh

Article

Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:

Insert

CD8-NbVHH05-GFP (UBE2J1 Human)

Tags

CD8 and GFP

Expression

Insect

Promoter

fly actin5C promoter

Available Since

June 30, 2021

Availability

Academic Institutions and Nonprofits only

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pTriEx-mVenus-Zdk1-Rac1 Q61L

Plasmid

#81137

Purpose

mammalian expression of Zdk1 tagged Rac1 Q61L

Depositor

Klaus Hahn

Article

Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

Inserts

Zdk1
Rac1 (RHOA Human)

Tags

mVenus

Expression

Bacterial, Insect, and Mamm…

Mutation

Q61L

Available Since

Aug. 29, 2016

Availability

Academic Institutions and Nonprofits only

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pKSB-609 U6-sgRNA1 ATCC GGAA

Plasmid

#173671

Purpose

To clone and express a gRNA in Anopheles

Depositor

Eric Marois

Article

Klug et al PLoS Pathog. 2022 Oct 12;18(10):e1010881

Type

Empty backbone

Use

CRISPR

Expression

Insect

Available Since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pMT-TRAF6-V5-His

Plasmid

#63752

Purpose

Expresses drosophila TRAF6 tagged with V5 and 6xHis epitopes, under control of metallothionein promoter

Depositor

Cyril Gueydan

Insert

TRAF6 (Traf6 Fly)

Tags

V5-His

Expression

Insect

Promoter

pMT

Available Since

April 6, 2015

Availability

Academic Institutions and Nonprofits only

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pCRISPaint-T2A-p65-3xP3-RFP

Plasmid

#127565

Purpose

CRISPaint universal donor plasmid for gene exon insertion. Encodes T2A-p65-SV40 and 3xP3-RFP visible eye marker.

Depositor

Norbert Perrimon

Article

Bosch et al Genetics. 2019 Nov 4. pii: genetics.119.

Insert

p65

Use

CRISPR

Expression

Insect

Available Since

Oct. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pOPINEneo-3C-GFP

Plasmid

#53534

Purpose

pTirex based GFP fusion vector for expression in mammalian, E. coli and insect cells using the baculovirus system

Depositor

Ray Owens

Insert

GFP

Tags

GFP-His8

Expression

Bacterial, Insect, and Mamm…

Available Since

June 17, 2014

Availability

Academic Institutions and Nonprofits only

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