4,908 results

1. Ac5-STABLE2-blast

   Plasmid

   #208034

   Purpose

   Enables expression of 2 ORFs (with or without tags) and selection with blasticidin in insect cells. Includes Flag-mCherry-T2A-GFP-T2A-blast with Actin5C promoter.

   Depositor

   James Sutherland, Rosa Barrio

   Article

   Gonzalez et al Sci Rep. 2011;1:75. doi: 10.1038/srep000

   Type

   Empty backbone

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Oct. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFastBac_Dual-GST-TEV-SINTBAD-EGFP (codon optimized Sf9)

   Plasmid

   #198035

   Purpose

   Used for the expression and purification of EGFP labelled SINTBAD (SMC Internal No. 1613)

   Depositor

   Sascha Martens

   Article

   Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

   Insert

   SINTBAD (TBKBP1 Human)
   

   Use

   Tags

   EGFP and GST-TEV

   Expression

   Insect

   Mutation

   Promoter

   Available since

   April 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. His-ZZ-TEV-SNAPf-DYNC2H1

   Plasmid

   #132524

   Purpose

   Full length human dynein 2 heavy chain DYNC2H1, Sf9 codon optimised. With 8xHis-ZZ tag, TEV cleavage site, SNAPf tag, precission cleavage site at N-terminus. Assembled from synthesised fragments

   Depositor

   Andrew Carter

   Article

   Toropova et al Nat Struct Mol Biol. 2019 Aug 26. pii: 1

   Insert

   DYNC2H1 (DYNC2H1 Human)
   

   Use

   Tags

   8x His, Linker-TEV-linker-TEV, Precission cleavag…

   Expression

   Insect

   Mutation

   Codon optimised for Sf9 expression

   Promoter

   Available since

   Oct. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. GFP-Rab18

   Plasmid

   #31740

   Depositor

   Ron Vale

   Article

   Huckaba et al J Biol Chem. 2011 Mar 4;286(9):7457-67.

   Insert

   Rab18 (Rab18 Fly)
   

   Use

   Tags

   GFP

   Expression

   Insect

   Mutation

   Promoter

   Actin

   Available since

   Sept. 21, 2011

   Availability

   Academic Institutions and Nonprofits only

5. pACU

   Plasmid

   #58373

   Purpose

   UAS vector to express gene of interest in Drosophila

   Depositor

   Yuh-Nung Jan, Chun Han

   Article

   Han et al Proc Natl Acad Sci U S A. 2011 Jun 7;108

   Type

   Empty backbone

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   June 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCRISPaint-T2A-Cas9-T2A-GFP-3xP3-RFP

   Plasmid

   #127560

   Purpose

   CRISPaint universal donor plasmid for gene exon insertion. Encodes T2A-Cas9-T2A-GFP-SV40 and 3xP3-RFP visible eye marker.

   Depositor

   Norbert Perrimon

   Article

   Bosch et al Genetics. 2019 Nov 4. pii: genetics.119.

   Insert

   Cas9-T2A-GFP
   

   Use

   CRISPR

   Tags

   Expression

   Insect

   Mutation

   EGFP-D102G see depositor comments below

   Promoter

   Available since

   July 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. OA-1050G (white)

   Plasmid

   #132420

   Purpose

   express arrays of gRNA targeting White under dU6-3 promoter

   Depositor

   Omar Akbari

   Article

   Buchman et al CRISPR J. 2020 Jun;3(3):164-176. doi: 10

   Insert

   white gRNA array
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   U6-3

   Available since

   Sept. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLib6.4B-Agam_695

   Plasmid

   #176668

   Purpose

   Expression of sgRNA under An. gambiae U6-2 (AGAP013695) promoter & delivery of D.mel-Actin5C::EBFP2-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

   Depositor

   Norbert Perrimon

   Article

   Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

   Type

   Empty backbone

   Use

   Crispr

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Nov. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. OA-1050E (Ubiq-CasRx)

   Plasmid

   #132416

   Purpose

   Express CasRx under Ubiquitin-63E promoter

   Depositor

   Omar Akbari

   Article

   Buchman et al CRISPR J. 2020 Jun;3(3):164-176. doi: 10

   Insert

   CasRx
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Ubiq

   Available since

   Sept. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAct-Fok1:dCas9

    Plasmid

    #62211

    Purpose

    Expresses Fok1:dCas9 under control of act5C promoter and SV40 3'UTR. For ubiquitous high specificity genome engineering in Drosophila melanogaster.

    Depositor

    Simon Bullock

    Insert

    Fok1-dCas9
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    act5C

    Available since

    March 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGE-attB-GMR_mNeonGreen::3XFLAG::dCrk

    Plasmid

    #131143

    Purpose

    Rescue construct for targeting into crk[ΔattP]. Contains 110bp upstream of transcription start site, mNeonGreen, 3XFLAG, and dCrk cDNA, 3' UTR and 99bp downstream.

    Depositor

    Mark Peifer

    Article

    Spracklen et al Mol Biol Cell. 2019 Jul 18:mbcE19050302.

    Insert

    mNeonGreen::3XFLAG::Crk (Crk Fly)
    

    Use

    Cre/Lox; Attb recombination site

    Tags

    3XFLAG and mNeonGreen

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Oct. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pQUAST-shibire (ts1)

    Plasmid

    #24362

    Depositor

    Liqun Luo

    Article

    Potter et al Cell. 2010 Apr 30. 141(3):536-48.

    Insert

    Shibire (ts1) (shi Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    May 10, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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13. pUAST cyto roGFP2-Orp1

    Plasmid

    #64996

    Purpose

    for expression of cytosolic roGFP2-Orp1 with the Gal4 expression system in drosophila

    Depositor

    Tobias Dick

    Article

    Albrecht et al Cell Metab. 2011 Dec 7;14(6):819-29. doi

    Insert

    Orp1 (HYR1 Budding Yeast)
    

    Use

    Tags

    roGFP2

    Expression

    Insect

    Mutation

    Promoter

    Available since

    June 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. 52 pLIB_3C_C_STREP

    Plasmid

    #160300

    Purpose

    C-term 3C cleavable 2xStrepII tag for insect cell expression

    Depositor

    John Weir

    Article

    Altmannova et al Protein Sci. 2020 Sep 21. doi: 10.1002/p

    Type

    Empty backbone

    Use

    Tags

    2xStrepII

    Expression

    Insect

    Mutation

    Promoter

    polyhydrin

    Available since

    Nov. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pIE-hα2M-H6

    Plasmid

    #128492

    Purpose

    Expresses human α2M (from S24 to N1473) in insect cells. With C-t H6x tag.

    Depositor

    F Xavier Gomis-Ruth

    Article

    Marino-Puertas et al Sci Rep. 2019 Jun 24;9(1):9186. doi: 10.

    Insert

    A2M (A2M Human)
    

    Use

    Tags

    His tag (6x)

    Expression

    Insect

    Mutation

    Promoter

    IE1

    Available since

    Aug. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTriEx-mCherry-LINXa3-nano

    Plasmid

    #73616

    Purpose

    Light-induced nuclear export switch fusion to nano for microscopy in mammalian cells.

    Depositor

    Brian Kuhlman

    Article

    Yumerefendi et al Nat Chem Biol. 2016 Jun;12(6):399-401. d

    Insert

    LINXa3-nano
    

    Use

    Tags

    mCherry

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    Promoter

    Available since

    April 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMT Stat92E-GFP

    Plasmid

    #126662

    Purpose

    Inducible expression of Stat92E-GFP in S2 cells

    Depositor

    Ron Vale

    Article

    Williamson et al J Cell Biol. 2018 Nov 5;217(11):3977-399

    Insert

    Stat29E
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. His-ZZ-TEV-BICDR1-GFP

    Plasmid

    #111657

    Purpose

    C-terminal GFP tag on BICDR1 for expression in Sf9 cells. 8xHis-ZZ-GFP N-terminal tag, TEV site to cleave 8xHis-ZZ.

    Depositor

    Andrew Carter

    Article

    Urnavicius et al Nature. 2018 Feb 7;554(7691):202-206. do

    Insert

    BICDR1-GFP (Bicdl1 Mouse)
    

    Use

    Tags

    8xHis-tag, GFP, TEV site, and ZZ

    Expression

    Insect

    Mutation

    Optimised for Sf9 expression

    Promoter

    Available since

    July 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAc5.1B-lambdaN-HA-DmPAN3-W707AG711E-dsRNAres_Y

    Plasmid

    #148087

    Purpose

    Insect Expression of DmPAN3-W707AG711E-dsRNAres

    Depositor

    Elisa Izaurralde

    Article

    Jonas et al Nat Struct Mol Biol. 2014 Jul;21(7):599-

    Insert

    DmPAN3-W707AG711E-dsRNAres (CG11486 Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pTriEx-mVenus-PA-Rac1-C450A

    Plasmid

    #22021

    Purpose

    Expression of Rac1 fused with light-insensitive LOV2 (C450A mutation)

    Depositor

    Klaus Hahn

    Article

    Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

    Insert

    PA-Rac1-C450A (RAC1 Avena sativa (oat), Human)
    

    Use

    Tags

    6X His and mVenus

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    LOV domain contains the light-insensitive C450A m…

    Promoter

    CMV, p10

    Available since

    Sept. 29, 2009

    Availability

    Academic Institutions and Nonprofits only