5,046 results
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Plasmid#205115PurposeQPRT protein expressionDepositorAvailable SinceOct. 13, 2023AvailabilityAcademic Institutions and Nonprofits only
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Ac5-STABLE1-blast
Plasmid#208033PurposeEnables expression of an ORF (with or without tags) and selection with blasticidin in insect cells. Includes GFP-T2A-blast with Actin5C promoter.DepositorTypeEmpty backboneExpressionInsectAvailable SinceOct. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAttB-Tub-HA-Wnk
Plasmid#203968PurposeFly transformation vector expressing indicated Wnk variant under tubulin promoter for ubiquitous expressionDepositorAvailable SinceOct. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUAS-kkv-R896K::NG
Plasmid#138957PurposeUsed to generate a transgene that will express an inactive fluorescent Chitin Synthase in Drosophila using Gal4DepositorArticleInsertkkv-R896K::NG (kkv Fly)
TagsNeon Green fused to C terminus of KkvExpressionInsectMutationR896KPromoterUASAvailable SinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUAS-FRT-GFP-FRT-uM-dCBEevoCDA1
Plasmid#205152PurposeInducible expression of dCBEevoCDA1 under control of Gal4/UAS and FLP/FRTDepositorInsertFRT-GFP-FRT-uORF-dCBEevoCDA1
UseCRISPRExpressionInsectPromoterUASTAvailable SinceSept. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
T6-pAC5.1 mEGFP-EcoRV
Plasmid#206489PurposeSchneider cell imagingDepositorTypeEmpty backboneTagsmEGFPExpressionInsectAvailable SinceSept. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDropIn-T2A-ZpGal4DBD
Plasmid#199199PurposeInsert to create ZpGal4DBD knock-ins, via Drop-In cloning, released via BsmBI digestDepositorInsertT2A-ZpGal4DBD
ExpressionInsectAvailable SinceAug. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDropIn-T2A-p65Zp
Plasmid#199200PurposeInsert to create p65Zp knock-ins, via Drop-In cloning, released via BsmBI digestDepositorInsertT2A-p65Zp
ExpressionInsectAvailable SinceAug. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
p10xUAS-WHaloCaMP1a-EGFP
Plasmid#205314PurposeExpression of WHaloCaMP1a-EGFP in Drosophila using GAL4 driverDepositorInsertWHaloCaMP1a-EGFP
ExpressionInsectAvailable SinceAug. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBVboostFGII WPRE 2A-CVB3
Plasmid#202076PurposeBacterial expression of 2A protease from coxsackievirus B3 (CVB3). Contains C-terminal His6-tag.DepositorInsertCVB3 2A protease with HisTag
Tags6xHis-tagExpressionBacterial, Insect, and Mamm…Promoterpolh, CAG, T7Available SinceAug. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pOET3-N-TEV-Twin-Strep
Plasmid#203323PurposeGenerate the baculovirus to express C-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-tagged protein (ENLYFQGS-WSHPQFEK-(GGGS)2-GGSA-WSHPQFEK) in insect cellsDepositorTypeEmpty backboneTagsTEV cleavable site (ENLYFQG) and Twin-strep-tagExpressionInsectAvailable SinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
OA-1055J
Plasmid#200251PurposepBac-U6-gRNA(Intersex)-3xp3-tdTomatoDepositorInsert2 gRNAs targeting Intersex gene
ExpressionInsectAvailable SinceMay 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
OA-1067B
Plasmid#200252PurposepBac-U6-gRNA(doublesex)-3xp3-tdTomatoDepositorInsert4 gRNAs targeting Doublesex
ExpressionInsectAvailable SinceMay 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFastBac-GST-TEV-mCherry-TBK1
Plasmid#198033PurposeUsed for the expression and purification of mCherry labelled TBK1 (SMC Internal No.: 1675)DepositorAvailable SinceApril 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
ptAD-seq-ubi63E-Gal4-DBD
Plasmid#111930PurposeVector to express tAD candidates from a library cloned from fragmented transcription factor coding sequences fused to the Gal4 DNA binding domain.DepositorInserttAD-seq Gal4-DBD
ExpressionInsectAvailable SinceApril 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL4.26_4xUAS_DSCP_GFP
Plasmid#111931PurposeVector to express GFP (under the control of the DSCP and an array of 4 UAS sites)DepositorInsert4xUAS GFP
ExpressionInsectAvailable SinceApril 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmGIGYF-WFFtoAAA_AH
Plasmid#148939PurposeInsect Expression of DmGIGYF-WFFtoAAADepositorInsertDmGIGYF-WFFtoAAA (Gyf Fly)
ExpressionInsectMutationone silent mutation compared to the sequence give…Available SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-Dm4EHP-W114A_AH
Plasmid#148909PurposeInsect Expression of Dm4EHP-W114ADepositorInsertDm4EHP-W114A (4EHP Fly)
ExpressionInsectMutation4 silent mutations compared to the sequence given…Available SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-Dm4EHP_AH
Plasmid#148914PurposeInsect Expression of Dm4EHPDepositorInsertDm4EHP (4EHP Fly)
ExpressionInsectMutation4 silent mutations compared to the sequence given…Available SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-Dm4EHP-W85A_AH
Plasmid#148917PurposeInsect Expression of Dm4EHP-W85ADepositorInsertDm4EHP-W85A (4EHP Fly)
ExpressionInsectMutation4 silent mutations compared to the sequence given…Available SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmGIGYF_AH
Plasmid#148918PurposeInsect Expression of DmGIGYFDepositorInsertDmGIGYF (Gyf Fly)
ExpressionInsectMutationone silent mutation compared to the sequence give…Available SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmGIGYF_641-1574_AH
Plasmid#148920PurposeInsect Expression of DmGIGYF_641-1574DepositorInsertDmGIGYF_641-1574 (Gyf Fly)
ExpressionInsectMutationone silent mutation compared to the sequence give…Available SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmeIF4G-trunc-V5His6_G
Plasmid#146392PurposeInsect Expression of DmeIF4G-trunc. *Note: this plasmid does not contain an HA-tag as the name implies.DepositorInsertDmeIF4G-trunc (eIF4G1 Fly)
ExpressionInsectMutation322-1666 truncated version of elF4G sequence with…Available SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-MS2-GST-V5His6_V
Plasmid#147757PurposeInsect Expression vectorDepositorTypeEmpty backboneExpressionInsectAvailable SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmAtx2_AE
Plasmid#148548PurposeInsect Expression of DmAtx2DepositorInsertDmAtx2 (Atx2 Fly)
ExpressionInsectMutationThree silent mutations and two deletions compared…Available SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-MBP_V
Plasmid#147736PurposeInsect Expression vectorDepositorTypeEmpty backboneExpressionInsectAvailable SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-HseIF4AI_T
Plasmid#147543PurposeInsect Expression of HseIF4AIDepositorInsertHseIF4AI (EIF4A1 Human)
ExpressionInsectMutation3 silent mutations compared to the sequence given…Available SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUAS-kkv-mos::NG
Plasmid#138958PurposeUsed to generate a transgene that will express a fluorescent Chitin Synthase in Drosophila where the active site is converted to the mosquito sequence. Expression by using Gal4DepositorArticleInsertkkv (NEWENTRY Fly)
UseUas transgene vectorTagsNeon Green fused to C terminus of KkvExpressionInsectMutation8 bp that are in the region that encodes the cata…PromoterUASAvailable SinceFeb. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGB-08-09-BECN1
Plasmid#187991PurposePlasmid for the protein expression of BECN1/Beclin1 in a bacterial and insect expression system. Internal_Reference: SMC1325DepositorInsertBECN1 (BECN1 Human)
ExpressionBacterial and InsectAvailable SinceFeb. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1C-RLuc-A90-HhR_O
Plasmid#147073PurposeInsect Expression of A90-HhRDepositorInsertA90-HhR
ExpressionInsectMutationA127 instead of A90 (see comments below)Available SinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only