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Plasmid#112645PurposeUbiquitous expression of one or multiple sgRNAs in Drosophila. Selection marker: Mini-white.DepositorInsertU6:3-tRNA-sgRNA expression cassette
UseTagsExpressionInsectMutationPromoterAvailable sinceAug. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJFRC201-10XUAS-FRT>STOP>FRT-myr::smGFP-HA
Plasmid#63166PurposeGAL4-responsive UAS "flp-On" spaghetti monster GFP-HA reporterDepositorInsert"spaghetti monster GFP-HA"
UseTagsN-myristoylationExpressionInsectMutationPromoterAvailable sinceMay 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
Brp-T2A-QF2w-Hsp70-3XP3-dsRed
Plasmid#141094PurposeTemplate plasmid for inserting QF2w into the Brp gene of Aedes aegypti with CRISPR/Cas9, to generate a pan-neuronal driver line.DepositorInsertbruchpilot
UseCRISPRTagsExpressionInsectMutationcoding sequence downstream of CRISPR cut site, sy…PromoterAvailable sinceSept. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
Ac5-STABLE2-neo
Plasmid#32426DepositorInsertFlag-mCherry-T2A-GFP-T2A-neo
UseTagsExpressionInsectMutationPromoterActin5CAvailable sinceOct. 6, 2011AvailabilityAcademic Institutions and Nonprofits only -
pAc V5 dDispatched WT HA
Plasmid#126405PurposeExpression of V5 dDispatched WT HA in Drosophila cells lines.DepositorInsertd-Dispatched1 (disp Fly)
UseTagsHAExpressionInsectMutationPromoterAvailable sinceJune 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFastBac1-6His-DHX9
Plasmid#211440Purposegeneration of Bacmid for insect cell expression of DHX9 N-terminally fused to 6XHis-TagDepositorInsertDHX9 (DHX9 Human)
UseTags6xHisExpressionInsectMutationPromoterAvailable sinceJan. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUAST-pHuji-GINKO2
Plasmid#177117PurposeGenetically encoded green fluorescent potassium ion indicator GINKO2 and red fluorescent pH indicator pHuji fusionDepositorInsertpHuji-GINKO2
UseTagsExpressionInsectMutationPromoterhsp70 promoterAvailable sinceFeb. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pACEBac1-PSMA1-PSMA2-PSMA3-PSMA4-PSMA5-PSMA6-PSMA7
Plasmid#214138PurposeInsect cell expression of human 20S CP alpha-subunitsDepositorInsertsUseTagsExpressionInsectMutationPromoterpolyhedrinAvailable sinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFastBac Dual α1B/β2B Tubulin
Plasmid#170553PurposeEncodes codon-optimized human α1B and β2B tubulin for expression in and purification from insect cells.DepositorUseTagsAP linker, GS linker (GGSGG), His10 tag, L21 enha…ExpressionInsectMutationPromoterAvailable sinceJune 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJFRC206-10XUAS-FRT>STOP>FRT-myr::smGFP-V5
Plasmid#63168PurposeGAL4-responsive UAS “flp-On” spaghetti monster GFP-V5 reporterDepositorInsert"spaghetti monster GFP-V5"
UseTagsN-myristoylationExpressionInsectMutationPromoterAvailable sinceJune 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFastBac_Dual_SNF2L-opt_p48
Plasmid#102243PurposeExpression of human codon optimized human SNF2L and pRbAp48 from pFastBac dualDepositorUseTagsExpressionInsectMutationcodon optimized for expressionPromoterPolyhedrin and p10Available sinceNov. 1, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
pJFRC203-10XUAS-FRT>STOP>FRT-myr::smGFP-cMyc
Plasmid#63167PurposeGAL4-responsive UAS “flp-On” spaghetti monster GFP-cMyc reporterDepositorInsert"spaghetti monster GFP-cMyc"
UseTagsN-myristoylationExpressionInsectMutationPromoterAvailable sinceJune 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHACK-QF2
Plasmid#80274PurposeQF2 HACK backbone. Contains QF2-hsp70, but no gRNAsDepositorInsertQF2
UseTagsExpressionInsectMutationPromoterAvailable sinceAug. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
LAMP1-GFP
Plasmid#16290DepositorInsertLAMP1/RE72002
UseTagsEGFPExpressionInsectMutationPromoterAc5Available sinceMarch 12, 2008AvailabilityAcademic Institutions and Nonprofits only -
pFastBac(His10-Lckg2a)
Plasmid#177891PurposeBaculo expression of His10 tag fused to human LckG2ADepositorInsertLck (LCK Human)
UseTags10xHis-TEVExpressionInsectMutationG2APromoterPolyhedrinAvailable sinceAug. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTriEx4-Cdc42-2G
Plasmid#68814PurposeFRET-based biosensor reporting on endogenous Cdc42 activation (for expression in E. coli, insect and vertebrate cells)DepositorInsertCdc42 activation reporter
UseTagsHis and MycExpressionBacterial, Insect, and Mamm…MutationPromoterCMVAvailable sinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJFRC208-10XUAS-FRT>STOP>FRT-myr::smGFP-FLAG
Plasmid#63169PurposeGAL4-responsive UAS “flp-On” spaghetti monster GFP-FLAG reporterDepositorInsert"spaghetti monster GFP-FLAG"
UseTagsN-myristoylationExpressionInsectMutationPromoterAvailable sinceMay 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pACEBac1-PSMB1-PSMB2-PSMB3-PSMB4
Plasmid#217895PurposeInsect cell expression of human 20S CP proteasome subunit beta type-1 (PSMB1), beta type-2 (PSMB2), beta type-3 (PSMB3), and beta type-4 (PSMB4)DepositorUseTagsExpressionInsectMutationPromoterAvailable sinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFp10
Plasmid#170460PurposeEarlyBac insect cell expression systemDepositorInsertnone
UseTagsExpressionInsectMutationPromoterdHsp70Available sinceJune 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDmAct5C::Cas9-2A-Neo
Plasmid#176679PurposeExpression of Drosophila codon optimized spCas9 under Drosophila Act5C promoterDepositorInsertspCas9; NeoR (aminoglycoside phosphotransferase from Tn5)
UseCrisprTagsExpressionInsectMutationDrosophila codon optimizedPromoterD. melanogaster Act5CAvailable sinceNov. 23, 2021AvailabilityAcademic Institutions and Nonprofits only