74,960 results

1. 8xNFAT-ZsG-hCD8

   Plasmid

   #153417

   Purpose

   NFAT-driven ZsGreen-1 reporter gene with human CD8ab gene

   Depositor

   Maki Nakayama

   Article

   Mann et al Front Immunol. 2020 Apr 9;11:633. doi: 1

   Insert

   8x of NFAT binding motif follwed by ZsGreen-1 fluorescent protein gene (Cd8a )
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. scaffold-linker-U6

   Plasmid

   #89639

   Purpose

   construction of paired sgRNAs driven by two U6 promoters. Vector itself only has one U6 promoter.

   Depositor

   Wensheng Wei

   Article

   Zhu et al Nat Biotechnol. 2016 Dec;34(12):1279-128

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   June 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCMV-T7-ABE8e-SpCas9(LWKYQS)-P2A-EGFP (AHK404)

   Plasmid

   #223113

   Purpose

   pCMV and pT7 Human expression plasmid for ABE8e using SpCas9 enzyme with LWKYQS amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

   Insert

   human codon optimized TadA8e-SpCas9(LWKYQS)-P2A-EGFP
   

   Use

   CRISPR

   Tags

   BPNLS-P2A-EGFP and BPNLS-TadA8e

   Expression

   Mammalian

   Mutation

   TadA8e mutations; nSpCas9(D10A/LWKYQS)=D1135L, S1…

   Promoter

   CMV and T7

   Available since

   April 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. LAMP1-RpHLuorin2

   Plasmid

   #171720

   Purpose

   Lysosomal expression of ratiometric pHLuorin2

   Depositor

   Geert van den Bogaart

   Article

   Linders et al ACS Chem Biol. 2022 Jan 21;17(1):240-251

   Insert

   Ratiometric pHLuorin2 fused to LAMP1 (LAMP1 Human)
   

   Use

   Tags

   ratiometric pHluorin2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. MIGR1

   Plasmid

   #27490

   Depositor

   Warren Pear

   Article

   Pear et al Blood. 1998 Nov 15. 92(10):3780-92.

   Type

   Empty backbone

   Use

   Retroviral

   Tags

   GFP and IRES

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 18, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pEGFP-LC3

   Plasmid

   #21073

   Purpose

   Mammalian expression of rat LC3 fused to EGFP

   Depositor

   Tamotsu Yoshimori

   Article

   Kabeya et al EMBO J. 2000 Nov 1. 19(21):5720-8.

   Insert

   microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   First ATG is not included.

   Promoter

   CMV

   Available since

   May 11, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. GFP-HSPA8

   Plasmid

   #121161

   Purpose

   Mammalian expression of GFP tagged HSPA8

   Depositor

   Christine Mayr

   Article

   Ma et al Cell. 2018 Oct 30. pii: S0092-8674(18)31

   Insert

   HSPA8 (HSPA8 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. CuO-LacI-NLS–SNAPf

   Plasmid

   #227368

   Purpose

   Lentiviral expression of LacI–SNAP

   Depositor

   David Pellman

   Article

   Papathanasiou et al Nature. 2023 Jul;619(7968):184-192. doi:

   Insert

   LacI NLS SNAPf
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pDAC439

   Plasmid

   #195237

   Purpose

   Mammalian expression of Csm complex from single promoter; RFP backbone (Used for RNA knockdown)

   Depositor

   Jennifer Doudna

   Article

   Colognori et al Nat Biotechnol. 2023 Jan 23. doi: 10.103

   Insert

   FLAG-NLS-Csm5-2A-FLAG-NLS-Csm4-2A-FLAG-NLS-Csm3-2A-FLAG-NLS-Csm2; RFP-2A-FLAG-NLS-Cas6-2A-FLAG-NLS-Csm1; crRNA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. p246 eSpCas9_2gRNAs_mH11

    Plasmid

    #164854

    Purpose

    gRNA vector for targeting mouse H11 locus

    Depositor

    Philip Jordan

    Article

    Pryzhkova et al Stem Cell Res. 2020 Nov 5;49:102078. doi

    Insert

    gRNAs for targeting mouse H11 locus
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    March 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. mAG-GAL3

    Plasmid

    #62734

    Purpose

    Mammalian vesicle leakage reporter

    Depositor

    Niels Geijsen

    Article

    D'Astolfo et al Cell. 2015 Apr 23;161(3):674-690. doi: 1

    Insert

    mAG-Galectin 3
    

    Use

    Lentiviral

    Tags

    mAzami-Green

    Expression

    Mammalian

    Mutation

    Promoter

    EF1-Alpha

    Available since

    April 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pGF-GAS4X

    Plasmid

    #225751

    Purpose

    This plasmid was designed to selectively monitor the activation of the STAT1 signaling pathway in NK cells, a pathway known to play a crucial role in regulating NK cells' activation.

    Depositor

    Eun Hee Han

    Article

    Min et al Commun Biol. 2024 Sep 30;7(1):1222. doi:

    Insert

    interferon gamma activating sequence (GAS) (STAT1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA 3.1(Hygro) myc-hPolQ-Flag

    Plasmid

    #73132

    Purpose

    To express in mammalian cells human PolQ

    Depositor

    Agnel Sfeir

    Article

    Mateos-Gomez et al Nature. 2015 Feb 12;518(7538):254-7. doi

    Insert

    Polymerase theta (POLQ Human)
    

    Use

    Tags

    Flag and myc

    Expression

    Mammalian

    Mutation

    Promoter

    cmv promoter

    Available since

    March 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pJSK659

    Plasmid

    #205128

    Purpose

    Expression of GFP-SnxA in mammalian cells

    Depositor

    Jason King

    Article

    Vines et al J Cell Biol. 2023 Sep 4;222(9):e20220907

    Insert

    SnxA
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pX333

    Plasmid

    #64073

    Purpose

    Vector for tandem expression of two sgRNAs from two independent U6 promoters. Cas9 is expressed by Cbh promoter.

    Depositor

    Andrea Ventura

    Article

    Maddalo et al Nature. 2014 Dec 18;516(7531):423-7. doi

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Tags

    3XFLAG-Cas9

    Expression

    Mammalian

    Mutation

    Promoter

    CBh; U6

    Available since

    May 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCI-neo FlucDM EGFP

    Plasmid

    #90172

    Purpose

    EGFP-tagged constructs to monitor the aggregation state of the sensors and the ability of cells to solubilize or degrade the aggregated proteins. FLuc contains double mutation

    Depositor

    Franz-Ulrich Hartl

    Article

    Gupta et al Nat Methods. 2011 Sep 4;8(10):879-84. do

    Insert

    FLuc EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    R188Q, R261Q in FLuc

    Promoter

    Available since

    Aug. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAAV-TRE-GCaMP6s-WPRE-sv40

    Plasmid

    #184414

    Purpose

    AAV expression of Fluorescent calcium reporter, GCaMP6s from TRE promoter

    Depositor

    Marylka Yoe Uusisaari

    Article

    Dorgans et al Front Cell Neurosci. 2022 Apr 27;16:8250

    Insert

    GCaMP6s
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE

    Available since

    June 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. phMYT1L-N174

    Plasmid

    #66809

    Purpose

    2nd generation lentiviral transfer plasmid. Expresses human MYT1L with G418 resistance

    Depositor

    Andrew Yoo

    Article

    Richner et al Nat Protoc. 2015 Oct;10(10):1543-55. doi

    Insert

    MYT1L (MYT1L Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Human EF1a

    Available since

    Sept. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pFX-EGFP-EEA1

    Plasmid

    #174451

    Purpose

    Express EGFP with early endosome-targeting sequence in mammalian cells

    Depositor

    Yusuke Ohba

    Article

    Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

    Insert

    EGFP with early endosome-targeting sequence
    

    Use

    Tags

    early endosomal antigen 1 (EEA1)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. H2B-GFP

    Plasmid

    #11680

    Depositor

    Geoff Wahl

    Article

    Kanda et al Curr Biol. 1998 Mar 26. 8(7):377-85.

    Insert

    Human histone H2B (H2BC11 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 31, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge