78,292 results

1. pLKO.1 Rheb1 shRNA #2

   Plasmid

   #26626

   Depositor

   David Sabatini

   Article

   Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

   Insert

   Rheb1 shRNA (RHEB Human)
   

   Use

   Lentiviral and RNAi

   Expression

   Mammalian

   Available Since

   Nov. 17, 2010

   Availability

   Academic Institutions and Nonprofits only

2. pCDH-puro-Bcl-XL

   Plasmid

   #46972

   Purpose

   lentiviral expression of Bcl-XL

   Depositor

   Jialiang Wang

   Article

   Cheng et al Clin Cancer Res. 2013 Apr 1;19(7):1748-5

   Insert

   BCL2L1 (BCL2L1 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 7, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV-DAAO

   Plasmid

   #60344

   Purpose

   Yeast D-Amino acid oxidase from Rhodotorula gracilis, optimized for mammalian expression.

   Depositor

   Vsevolod Belousov

   Article

   Matlashov et al Antioxid Redox Signal. 2014 Mar 1;20(7):

   Insert

   DAAO
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1 FLAG-BACH2

   Plasmid

   #232275

   Purpose

   Expression of FLAG-tagged BACH2 (H. sapiens) in human cell lines

   Depositor

   Michele Pagano

   Article

   Cao et al Cell. 2024 Oct 30:S0092-8674(24)01188-7.

   Insert

   BACH2 (BACH2 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV4-BlaM-Vpr

   Plasmid

   #21950

   Depositor

   Warner Greene

   Article

   Cavrois et al Nat Biotechnol. 2002 Nov . 20(11):1151-4

   Insert

   BlaM-Vpr (vpr Fusion of BlaM and HIV-1 Vpr)
   

   Expression

   Mammalian

   Available Since

   Oct. 1, 2009

   Availability

   Academic Institutions and Nonprofits only

6. L_NLS 41kDa

   Plasmid

   #201342

   Purpose

   Nucleocytoplasmic transport probe

   Depositor

   Pere Roca-Cusachs

   Article

   Andreu et al Nat Cell Biol. 2022 Jun;24(6):896-905. d

   Insert

   (NLS)SV40A4-EGFP-2PrA
   

   Expression

   Mammalian

   Available Since

   June 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. CMV PGK1-3xHA

   Plasmid

   #220913

   Purpose

   Expresses PGK1-3xHA

   Depositor

   Timothy Ryan

   Article

   Kokotos et al Sci Adv. 2024 Aug 23;10(34):eadn6016. do

   Insert

   PGK1-3xHA (PGK1 Human)
   

   Use

   Affinity Reagent/ Antibody

   Tags

   3xHA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHR_PGK_ BCAN 13134 VLVH synNotch

   Plasmid

   #247570

   Purpose

   Expresses a synNotch receptor binding to BCAN

   Depositor

   Wendell Lim

   Article

   Simic et al Science. 2024 Dec 6;386(6726):eadl4237.

   Insert

   Expresses a synNotch receptor binding to BCAN
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Nov. 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. tetO-ID1

   Plasmid

   #170692

   Purpose

   doxycycline-inducible overexpression of ID1

   Depositor

   Paul Burridge

   Article

   Romero-Tejeda et al J Mol Cell Cardiol. 2023 Jul 6:S0022-282

   Insert

   ID1 (ID1 Human)
   

   Use

   Lentiviral; Doxycycline inducible

   Expression

   Mammalian

   Promoter

   TRE promoter, Tet-ON

   Available Since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. TRPML1-G-GECO1.2-ERES

    Plasmid

    #207144

    Purpose

    Fusion of Ca2+ reporter and lysosomal Ca2+-permeable channel, with ER-export signal

    Depositor

    Antony Galione

    Article

    Davis et al EMBO J. 2020 Jul 15;39(14):e104058. doi:

    Insert

    Mcoln1 (MCOLN1 Human)
    

    Tags

    ER-Export Signal (ERES) and G-GECO1.2

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.2/NS2A

    Plasmid

    #120818

    Purpose

    Ectopic expression of ZIKV protein

    Depositor

    Jan Rehwinkel

    Article

    Hertzog et al Eur J Immunol. 2018 Mar 23. doi: 10.1002

    Insert

    NS2A ZIKV
    

    Tags

    V5

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCEP4-HA-CCR5

    Plasmid

    #98950

    Purpose

    Mammalian expression plasmid for N-terminal HA-tagged human CCR5

    Depositor

    Erik Procko

    Article

    Heredia et al J Immunol. 2018 Apr 20. pii: jimmunol.18

    Insert

    CCR5 (CCR5 Human)
    

    Tags

    HA (YPYDVPDYA) epitope tag and Signal/leader sequ…

    Expression

    Mammalian

    Mutation

    Full-length, wildtype

    Promoter

    CMV

    Available Since

    April 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. YFP NLS Beta-Actin G13R

    Plasmid

    #60615

    Purpose

    Encodes YFP and NLS tagged beta-actin with the G13R depolymerization mutation

    Depositor

    Primal de Lanerolle

    Article

    Chang et al Proc Natl Acad Sci U S A. 2011 May 24;10

    Insert

    Beta-actin (ACTB Human)
    

    Tags

    EYFP and NLS

    Expression

    Mammalian

    Mutation

    G13R

    Available Since

    Nov. 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV/D377Y-mPCSK9

    Plasmid

    #58376

    Purpose

    Expresses GoF mutant murine PCSK9 to be used for rAAV8-mediated gene transfer, hypercholesterolemia and atherosclerosis

    Depositor

    Jacob Bentzon

    Article

    Bjorklund et al Circ Res. 2014 May 23;114(11):1684-9. do

    Has Service

    AAV8

    Insert

    proprotein convertase subtilisin/kexin type 9 (Pcsk9 Mouse)
    

    Use

    AAV

    Expression

    Mammalian

    Mutation

    D377Y

    Promoter

    HCRApoE/hAAT

    Available Since

    Aug. 26, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pShuttle-FEN1DA

    Plasmid

    #35028

    Depositor

    Sheila Stewart

    Article

    Saharia et al J Biol Chem. 2010 Aug 27;285(35):27057-6

    Insert

    FEN1DA (FEN1 Human)
    

    Use

    Adenoviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    D181A--NUCLEASE DEAD POINT MUTANT

    Promoter

    CMV

    Available Since

    Aug. 28, 2012

    Availability

    Academic Institutions and Nonprofits only

16. TDP-REGv2(mScarlet-FLAG)#7 in pTwist-CMV backbone

    Plasmid

    #216152

    Purpose

    Expresses mScarlet in cells with TDP-43 loss-of-function. This plasmid has one of the best dynamic ranges of those tested (~100-fold) in SK-N-BE2 cells. It uses a cryptic exon. [Code 'A11'] Note: It is not recommended to produce lentiviruses containing TDP-REG sequences – see Depositor Comments

    Depositor

    Pietro Fratta

    Article

    Wilkins et al Science. 2024 Oct 4;386(6717):61-69. doi

    Insert

    mScarlet with cryptic exon and C-terminal FLAG
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pShuttle-FEN1deltaP

    Plasmid

    #35030

    Depositor

    Sheila Stewart

    Article

    Saharia et al J Biol Chem. 2010 Aug 27;285(35):27057-6

    Insert

    FEN1DELTAP (FEN1 Human)
    

    Use

    Adenoviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    8 AMINO ACID DELETION OF P BOX (amino acids 337–3…

    Promoter

    CMV

    Available Since

    Sept. 10, 2012

    Availability

    Academic Institutions and Nonprofits only

18. pcDNA3-Ha-Brca1 S1423A

    Plasmid

    #32533

    Depositor

    Michael Kastan

    Article

    Xu et al Cancer Res. 2002 Aug 15;62(16):4588-91.

    Insert

    BRCA1 (BRCA1 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    S1423A

    Promoter

    CMV/rabbit B-globin

    Available Since

    Oct. 19, 2011

    Availability

    Academic Institutions and Nonprofits only

19. AAVS1_Puro_Tet3G_3xFLAG_Twin_Strep

    Plasmid

    #92099

    Purpose

    Doyon lab Tandem-Affinity Purification Following Nuclease-Driven Gene Addition to the AAVS1 Genomic Safe Harbor Locus. Transgene expression is controlled by an Auto-Regulated Tet-On 3G System.

    Depositor

    Yannick Doyon

    Article

    Dalvai et al Cell Rep. 2015 Oct 7. pii: S2211-1247(15

    Type

    Empty backbone

    Use

    CRISPR and TALEN; Zfn

    Expression

    Mammalian

    Promoter

    Tet-On 3G Bidirectional

    Available Since

    June 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLenti-Cas9-GFP

    Plasmid

    #86145

    Purpose

    Lentiviral vector expressing Cas9-GFP

    Depositor

    David Sabatini

    Article

    Wang et al Cell. 2017 Feb 1. pii: S0092-8674(17)300

    Insert

    Cas9-GFP
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG and GFP

    Expression

    Mammalian

    Promoter

    EFS

    Available Since

    Feb. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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