81,755 results

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pAC152-dual-dCas9VP64-sgExpression

Plasmid

#48238

Purpose

Dual expression construct expressing both dCas9VP64 and sgRNA from separate promoters

Depositor

Rudolf Jaenisch

Article

Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

Insert

dCas9

Use

CRISPR

Tags

HA Tag, HA-Tag, and VP64

Expression

Mammalian

Mutation

D10A;H840A

Available Since

Sept. 27, 2013

Availability

Academic Institutions and Nonprofits only

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pLenti6.3/V5-DEST-GFP

Plasmid

#40125

Depositor

Lynda Chin

Article

Quayle et al Neuro Oncol. 2012 Nov;14(11):1325-31. do

Insert

GFP

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

July 16, 2013

Availability

Academic Institutions and Nonprofits only

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ankG-mCherry

Plasmid

#42566

Depositor

Benedicte Dargent

Article

Leterrier et al Proc Natl Acad Sci U S A. 2011 May 24;10

Insert

ankyrin G (Ank3 Rat)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

April 26, 2013

Availability

Academic Institutions and Nonprofits only

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FLAG-MATR3

Plasmid

#32880

Depositor

Yossi Shiloh

Article

Salton et al PLoS One. 2011;6(8):e23882. Epub 2011 Au

Insert

MATR3 Matrin3 (MATR3 Human)

Tags

FLAG

Expression

Bacterial and Mammalian

Promoter

CMV

Available Since

Jan. 6, 2012

Availability

Academic Institutions and Nonprofits only

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CMV-G-GECO1.1

Plasmid

#32445

Purpose

Mammalian expression of green fluorescent genetically encoded Ca2+ indicator for optical imaging (version 1.1)

Depositor

Robert Campbell

Article

Zhao et al Science. 2011 Sep 30;333(6051):1888-91.

Insert

G-GECO1.1

Expression

Mammalian

Mutation

GCaMP3 K69E/N77Y/D86G/K119I/L173Q/K380N/S404G/E43…

Promoter

CMV

Available Since

Sept. 29, 2011

Availability

Academic Institutions and Nonprofits only

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eTC GFP beta-actin full length

Plasmid

#27123

Depositor

Robert Singer

Article

Rodriguez et al J Cell Biol. 2006 Oct 9. 175(1):67-76.

Insert

beta-actin (ACTB Human)

Tags

EGFP and TC tag

Expression

Mammalian

Mutation

E237A

Available Since

Jan. 14, 2011

Availability

Academic Institutions and Nonprofits only

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pLenti-CaMKIIa-ChETA-EYFP

Plasmid

#26967

Purpose

3rd gen lentiviral expression of humanized ChR2 with E123T/H134R mutations fused to EYFP driven by CaMKIIa promoter for optogenetic activation

Depositor

Karl Deisseroth

Article

Gunaydin et al Nat Neurosci. 2010 Mar;13(3):387-92. doi

Insert

hChR2(E123T/H134R)-EYFP

Use

Lentiviral

Tags

EYFP

Expression

Mammalian

Mutation

E123T

Promoter

CaMKIIa

Available Since

Jan. 11, 2011

Availability

Academic Institutions and Nonprofits only

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pLJM1 Flag Raptor

Plasmid

#26633

Depositor

David Sabatini

Article

Sancak et al Cell. 2010 Apr 16. 141(2):290-303.

Insert

raptor (RPTOR Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Available Since

Oct. 22, 2010

Availability

Academic Institutions and Nonprofits only

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pcDNA3-ClopHensor

Plasmid

#25938

Depositor

Daniele Arosio

Article

Arosio et al Nat Methods. 2010 Jul . 7(7):516-8.

Insert

ClopHensor

Tags

Strep-II

Expression

Mammalian

Mutation

E2GFP is EGFP-T203Y

Available Since

Sept. 13, 2010

Availability

Academic Institutions and Nonprofits only

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FLHKII-pGFPN3

Plasmid

#21920

Depositor

Hossein Ardehali

Article

Sun et al Mol Cell Biol. 2008 Feb . 28(3):1007-17.

Insert

Full length human HKII cDNA in pGFP-N3 plasmid (HK2 Human)

Tags

GFP

Expression

Mammalian

Mutation

This is the full length human HKII cDNA sequence …

Available Since

Sept. 17, 2009

Availability

Academic Institutions and Nonprofits only

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pCMVTAG-NEMO

Plasmid

#11970

Depositor

Jon Ashwell

Article

Wu et al Nat Cell Biol. 2006 Apr . 8(4):398-406.

Insert

NEMO (IKBKG Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 21, 2006

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 myc TSC1

Plasmid

#12133

Depositor

Cheryl Walker

Article

Cai et al J Cell Biol. 2006 Apr 24. 173(2):279-89.

Insert

TSC1 (TSC1 Human)

Tags

his and myc

Expression

Mammalian

Available Since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only

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aP2-luciferase

Plasmid

#8858

Depositor

Bruce Spiegelman

Article

Graves et al Mol Cell Biol. 1992 Mar . 12(3):1202-8.

Insert

aP2 enhancer / promoter (Fabp4 Mouse)

Use

Luciferase

Tags

luciferase reporter

Expression

Mammalian

Available Since

Feb. 13, 2006

Availability

Academic Institutions and Nonprofits only

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1099 pcDNA GFP FKHR AAA

Plasmid

#9023

Depositor

William Sellers

Article

Nakamura et al Mol Cell Biol. 2000 Dec . 20(23):8969-82

Insert

FKHR AAA (FOXO1 Human)

Tags

GFP

Expression

Mammalian

Mutation

T24A S256A S319A

Available Since

Dec. 7, 2005

Availability

Academic Institutions and Nonprofits only

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1318 pcDNA3 flag FKHRL1

Plasmid

#10708

Depositor

William Sellers

Article

Ramaswamy et al Cancer Cell. 2002 Jul . 2(1):81-91.

Insert

FKHRL1 (FOXO3 Human)

Tags

flag

Expression

Mammalian

Available Since

Nov. 2, 2005

Availability

Academic Institutions and Nonprofits only

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pBABE-puro-DN-hTERT

Plasmid

#1775

Depositor

Bob Weinberg

Article

Hahn et al Nat Med 1999 Oct;5(10):1164-70.

Insert

hTERT (TERT Human)

Use

Retroviral

Expression

Mammalian

Mutation

Dominant negative, 2 base pair mutation, catalyti…

Available Since

June 23, 2005

Availability

Academic Institutions and Nonprofits only

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pCMV-MMLVgag-3xNES-ABE8e

Plasmid

#181751

Purpose

MMLV-Gag fused to 3xNES-ABE8e with TSTLLMENSS cleavage site between Gag-NES and ABE. For production of virus like particles with base-editor RNP cargo.

Depositor

David Liu

Article

Banskota et al Cell. 2022 Jan 7. pii: S0092-8674(21)014

Insert

MMLVgag-3xNES-ABE8e

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pEGFP_mNG2(11)_Clathrin light chain

Plasmid

#82608

Purpose

Expresses mNG2(11) tagged clathrin light chain in mammalian cells

Depositor

Bo Huang

Article

Feng et al Nat Commun. 2017 Aug 29;8(1):370. doi: 1

Insert

mNG2(11)_Clathrin light chain

Expression

Mammalian

Available Since

May 15, 2019

Availability

Academic Institutions and Nonprofits only

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pSFFV_sfCherry2(1-10)

Plasmid

#82603

Purpose

Lentiviral vector for constitutive expression of sfCherry2(1-10)

Depositor

Bo Huang

Article

Feng et al Nat Commun. 2017 Aug 29;8(1):370. doi: 1

Insert

sfCherry2(1-10)

Use

Lentiviral

Expression

Mammalian

Mutation

changed Glu 118 to Gln, changed Thr 128 to Ile

Promoter

SFFV

Available Since

Jan. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pClneoMyc-LATS2

Plasmid

#66852

Purpose

Express Myc-tagged LATS2 in mammalian cells

Depositor

Yutaka Hata

Article

Bao et al J Biochem. 2011 Aug;150(2):199-208. doi:

Insert

LATS2 (LATS2 Human)

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 17, 2015

Availability

Academic Institutions and Nonprofits only

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