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pLVX-EF1alpha-SARS-CoV-2-nsp7-2xStrep-IRES-Puro

Plasmid

#141373

Purpose

Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus

Depositor

Nevan Krogan

Publication

Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

Insert

SARS-CoV-2-nsp7 (ORF1ab SARS-CoV-2)

Use

Lentiviral

Tags

2xStrep

Expression

Mammalian

Mutation

human codon optimized

Promoter

EF1alpha

Available since

April 6, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

10 Articles

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pT3-EF1A-MYC-IRES-luc

Plasmid

#129775

Purpose

Transposon based vector that expresses human MYC followed by IRES and firefly luciferase

Depositor

Amaia Lujambio

Publication

Ruiz de Galarreta et al Cancer Discov. 2019 Jun 11. pii: 2159-82

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

human MYC (MYC Human)

Tags

luciferase

Expression

Mammalian

Promoter

EF1A

Available since

Feb. 13, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

16 Articles

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pH2B-emiRFP670

Plasmid

#136571

Purpose

Histone 2B labeling with near-infrared fluorescent protein emiRFP670 (enhanced miRFP670)

Depositor

Vladislav Verkhusha

Publication

Matlashov et al Nat Commun. 2020 Jan 13;11(1):239. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

H2B-emiRFP670

Tags

emiRFP670

Expression

Mammalian

Promoter

CMV

Available since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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CMV-hRad51

Plasmid

#125570

Purpose

Expresses the Rad51 protein in mammalian cells under CMV promoter

Depositor

David Liu

Publication

Rees et al Nat Commun. 2019 May 17;10(1):2212. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Rad51 (RAD51 Human)

Use

CRISPR

Expression

Mammalian

Available since

May 21, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pAAV-M13-TEV-C-P2A-TdTomato

Plasmid

#92391

Purpose

produces AAV expressing Cal-light M13-TevC component and TdTomato

Depositor

Hyungbae Kwon

Publication

Lee et al Nat Biotechnol. 2017 Jun 26. doi: 10.103

Insert

M13-TEV-C-P2A-TdTomato

Use

AAV and Synthetic Biology

Tags

TdTomato

Expression

Mammalian

Promoter

human synapsin

Available since

June 26, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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GFP-Rab27A

Plasmid

#89237

Purpose

Expression of GFP-tagged human Rab27A in mammalian cells

Depositor

William Gahl

Publication

Westbroek et al Mol Genet Metab. 2008 Jun;94(2):248-54.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

human Rab27A (RAB7A Human)

Tags

eGFP

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

April 25, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pTetON-EGFP

Plasmid

#89453

Purpose

Tet/Dox-inducible expression of EGFP in mammalian cells (control)

Depositor

Brad Zuchero

Publication

Harterink et al Nat Methods. 2017 Apr 10. doi: 10.1038/n

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

EGFP

Expression

Mammalian

Promoter

TRE3G

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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mCherry-DFCP1

Plasmid

#86746

Purpose

N-terminal mCherry-tagged protein expression in mammalian cells

Depositor

Do-Hyung Kim

Publication

Kim et al Mol Cell. 2015 Jan 22;57(2):207-18. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

human DFCP1 (ZFYVE1 Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available since

Jan. 25, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

12 Articles

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pAAVS1-P-CAG-DEST

Plasmid

#80490

Purpose

Gateway Destination donor vector for AAVS1 targeting (puromycin selection)

Depositor

Knut Woltjen

Publication

Oceguera-Yanez et al Methods. 2015 Dec 18. pii: S1046-2023(15

Type

Empty backbone

Use

Donor vector (human)

Expression

Mammalian

Available since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

12 Articles

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pTriEx-mCherry-LOV2

Plasmid

#81041

Purpose

Mammalian or insect expression for wt LOV2

Depositor

Klaus Hahn

Publication

Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

LOV2

Tags

mCherry

Expression

Bacterial, Insect, and Mamm…

Promoter

CMV, p10

Available since

Aug. 17, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pLPCX roGFP2-Orp1

Plasmid

#64991

Purpose

Mammalian expression of cytosolic roGFP2-Orp1 (retroviral vector)

Depositor

Tobias Dick

Publication

Gutscher et al J Biol Chem. 2009 Nov 13;284(46):31532-4

Insert

Orp1 (HYR1 Budding Yeast)

Use

Retroviral

Tags

roGFP2

Expression

Mammalian

Available since

June 16, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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pLL7.0: Venus-iLID-Mito (From ActA)

Plasmid

#60413

Purpose

Mammalian Expression of Venus-iLID-Mito

Depositor

Brian Kuhlman

Publication

Guntas et al Proc Natl Acad Sci U S A. 2015 Jan 6;112

Insert

Venus-iLID-Mito

Use

Cre/Lox and Lentiviral

Tags

Venus

Expression

Mammalian

Promoter

CMV

Available since

Feb. 27, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

14 Articles

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mOrange2-N1

Plasmid

#54568

Purpose

Localization: N1 Cloning Vector, Excitation: 549, Emission: 565

Depositor

Michael Davidson, Nathan Shaner, Roger Tsien

Publication

Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

Type

Empty backbone

Tags

mOrange2

Expression

Mammalian

Available since

June 12, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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mKO2-N1

Plasmid

#54625

Purpose

Localization: N1 Cloning Vector, Excitation: 551, Emission: 565

Depositor

Michael Davidson, Atsushi Miyawaki

Publication

Sakaue-Sawano et al Cell. 2008 Feb 8;132(3):487-98. doi: 10.

Type

Empty backbone

Tags

mKO2

Expression

Mammalian

Available since

June 12, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles

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pGFP Cortactin

Plasmid

#50728

Purpose

mammalian expression of GFP-Cortactin. Note- This plasmid contains GFP21, which has a sequence similar to YFP, but emission/excitation similar to GFP.

Depositor

Kenneth Yamada

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Cortactin (CTTN Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles

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CFP-Parkin

Plasmid

#47560

Purpose

CFP tagged Parkin for colocalization study

Depositor

Richard Youle

Publication

Narendra et al J Cell Biol. 2008 Dec 1. 183(5):795-803.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Parkin (PRKN Human)

Tags

CFP

Expression

Mammalian

Promoter

CMV

Available since

Sept. 4, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pEGFP-N1-delta30-TFEB

Plasmid

#44445

Depositor

Shawn Ferguson

Publication

Roczniak-Ferguson et al Sci Signal. 2012 Jun 12;5(228):ra42.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

TFEB (TFEB Human)

Tags

GFP

Expression

Mammalian

Mutation

Deleted amino acids 1-30; H32D

Available since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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G6PD/pRK5

Plasmid

#41521

Depositor

Xiaolu Yang

Publication

Jiang et al Nat Cell Biol. 2011 Mar;13(3):310-6. Epu

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

glucose-6-phosphate dehydrogenase (G6PD Human)

Expression

Mammalian

Promoter

CMV

Available since

Jan. 14, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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pMXs-puro GFP-DFCP1

Plasmid

#38269

Depositor

Noboru Mizushima

Publication

Itakura et al Autophagy. 2010 Aug;6(6):764-76.

Insert

Zinc finger FYVE domain-containing protein 1 (Zfyve1 Mouse)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Available since

Aug. 16, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

20 Articles

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pT7-EGFP-C1-HsNot1

Plasmid

#37370

Depositor

Elisa Izaurralde

Publication

Braun et al Mol Cell. 2011 Oct 7;44(1):120-33.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

HsNot1 (CNOT1 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available since

Aug. 16, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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