78,002 results

1. TDP-REGv2(mScarlet-FLAG)#7 in pTwist-CMV backbone

   Plasmid

   #216152

   Purpose

   Expresses mScarlet in cells with TDP-43 loss-of-function. This plasmid has one of the best dynamic ranges of those tested (~100-fold) in SK-N-BE2 cells. It uses a cryptic exon. [Code 'A11'] Note: It is not recommended to produce lentiviruses containing TDP-REG sequences – see Depositor Comments

   Depositor

   Pietro Fratta

   Article

   Wilkins et al Science. 2024 Oct 4;386(6717):61-69. doi

   Insert

   mScarlet with cryptic exon and C-terminal FLAG
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCX459

   Plasmid

   #229214

   Purpose

   CRISPR array plasmid for MAP1B (Spacers 1-24)

   Depositor

   Jennifer Doudna

   Article

   Xia et al Nat Biotechnol. 2025 Feb 18. doi: 10.103

   Insert

   CRISPR array plasmid for MAP1B (Spacers 1-24)
   

   Expression

   Mammalian

   Available Since

   Feb. 18, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. AbVec1.1-IGLC2-XhoI

   Plasmid

   #99575

   Purpose

   Expression of immunoglobulin light chains in mammalian cells, human lambda 2 constant region

   Depositor

   Hedda Wardemann

   Article

   Tiller et al J Immunol Methods. 2008 Jan 1;329(1-2):1

   Type

   Empty backbone

   Expression

   Mammalian

   Promoter

   hCMV IE1 gene promoter

   Available Since

   Aug. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pT3-EF1aH NICD1

   Plasmid

   #86500

   Purpose

   Mammalian expression of Myc tagged NICD1

   Depositor

   Xin Chen

   Article

   Fan et al J Clin Invest. 2012 Aug 1;122(8):2911-5.

   Insert

   Notch1 (C-term) (Notch1 Mouse)
   

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Cytoplasmic domain only (AA 1753-2531)

   Promoter

   EF1a

   Available Since

   April 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMH-Halo tag

   Plasmid

   #154144

   Purpose

   Gateway compatible mammalian expression plasmid for halo tag fusion proteins

   Depositor

   Michael Huen

   Type

   Empty backbone

   Tags

   Halo tag

   Expression

   Mammalian

   Available Since

   Oct. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. LAMP1-RpHLuorin2

   Plasmid

   #171720

   Purpose

   Lysosomal expression of ratiometric pHLuorin2

   Depositor

   Geert van den Bogaart

   Article

   Linders et al ACS Chem Biol. 2022 Jan 21;17(1):240-251

   Insert

   Ratiometric pHLuorin2 fused to LAMP1 (LAMP1 Human)
   

   Tags

   ratiometric pHluorin2

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. ND6-DdCBE-left side TALE

   Plasmid

   #157842

   Purpose

   expresses left-side TALE–DddAtox half of ND6-DdCBE in mammalian cells

   Depositor

   David Liu

   Article

   Mok et al Nature. 2020 Jul 8. pii: 10.1038/s41586-

   Insert

   SOD2 MTS–3x-HA–ND6 left tale–G1397 DddA-N–UGI–SOD2 3'UTR
   

   Expression

   Mammalian

   Available Since

   Aug. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. EndophilinA1-mRuby2

   Plasmid

   #171945

   Purpose

   Expresses Endophilin A1 in mammalian cells

   Depositor

   Cheng-han Yu

   Article

   Feng et al Proc Natl Acad Sci U S A. 2021 May 11;11

   Insert

   Endophilin A1 (SH3GL2 Human)
   

   Tags

   mRuby2

   Expression

   Mammalian

   Available Since

   July 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. nuclear cpVenus control

   Plasmid

   #186790

   Purpose

   Identical to nuclear NAD+ biosensor, sans bipartite NAD+-binding domain modeled from bacterial DNA ligase

   Depositor

   Michael Cohen

   Article

   Cambronne et al Science. 2016 Jun 17;352(6292):1474-7. d

   Insert

   nuclear cpVenus control
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   July 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. ND6-DdCBE-right side TALE

    Plasmid

    #157841

    Purpose

    expresses right-side TALE–DddAtox half of ND6-DdCBE in mammalian cells

    Depositor

    David Liu

    Article

    Mok et al Nature. 2020 Jul 8. pii: 10.1038/s41586-

    Insert

    COX8A MTS–3xFLAG–ND6 right TALE–G1397 DddA-C–UGI–ATP5B 3'UTR
    

    Expression

    Mammalian

    Available Since

    Aug. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. Red Glifon Nega

    Plasmid

    #163117

    Purpose

    Red fluorescent protein-based glucose negative control sensor (does not respond to glucose) for mammalian cells

    Depositor

    Takashi Tsuboi

    Article

    Mita et al Cell Chem Biol. 2021 Jun 29. pii: S2451-

    Insert

    Red Glifon Nega
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. GW1-pHRed

    Plasmid

    #31473

    Purpose

    genetically encoded pH sensor made from mutagenesis of the red fluorescent protein mKeima

    Depositor

    Gary Yellen

    Article

    Tantama et al J Am Chem Soc. 2011 Jul 6. 133(26):10034

    Insert

    pHRed
    

    Expression

    Mammalian

    Available Since

    July 21, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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13. Rosa26 mT/mG

    Plasmid

    #17787

    Depositor

    Liqun Luo

    Article

    Muzumdar et al Genesis. 2007 Sep . 45(9):593-605.

    Insert

    Rosa26 mT/mG
    

    Use

    Cre/Lox and Mouse Targeting

    Tags

    membrane tag

    Expression

    Mammalian

    Available Since

    May 14, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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14. mEmerald-Rab7a-7

    Plasmid

    #54244

    Purpose

    Localization: Ras GTPase, Excitation: 487, Emission: 509

    Depositor

    Michael Davidson

    Insert

    Rab7a (RAB7A Human)
    

    Tags

    mEmerald

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 25, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pCI-neo-hCG

    Plasmid

    #16574

    Depositor

    Bert Vogelstein

    Article

    Shih et al Nat Med. 2000 Jun . 6(6):711-4.

    Insert

    Beta-hCG (CGB3 Human)
    

    Expression

    Mammalian

    Available Since

    April 7, 2008

    Availability

    Academic Institutions and Nonprofits only

16. V5-miniTurbo-NES_pCDNA3

    Plasmid

    #107170

    Purpose

    expresses V5-tagged miniTurbo in the mammalian cytosol

    Depositor

    Alice Ting

    Article

    Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

    Insert

    miniTurbo (BirA mutant)
    

    Expression

    Mammalian

    Mutation

    aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

    Available Since

    May 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. Halo-VSVG-RUSH

    Plasmid

    #166907

    Purpose

    RUSH cargo: Str-Ii_IRES_VSVG-SBP-Halo

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Weigel et al Cell. 2021 Apr 29;184(9):2412-2429.e16.

    Insert

    HaloTag
    

    Tags

    HaloTag

    Expression

    Mammalian

    Available Since

    April 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. TFORF2029

    Plasmid

    #143566

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    SMARCA5 (SMARCA5 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Feb. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA4/TO/cmycDrosha

    Plasmid

    #10828

    Depositor

    Thomas Tuschl

    Article

    Landthaler et al Curr Biol. 2004 Dec 14. 14(23):2162-7.

    Insert

    Drosha (DROSHA Human)
    

    Tags

    myc

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 7, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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20. Lenti-Trono-EFS-Blast-P2A-TurboRFP

    Plasmid

    #228893

    Purpose

    Cloning and expression of gRNAs or pegRNAs with EcoRI/Esp3I insertion.

    Depositor

    Francisco J Sánchez-Rivera

    Article

    Gould et al Nat Biotechnol. 2024 Mar 12. doi: 10.103

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Dec. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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