77,958 results

1. pX330A-FBL/PITCh

   Plasmid

   #63671

   Purpose

   Expresses Cas9 nuclease, FBL-specific gRNA, and the PITCh-gRNA

   Depositor

   Takashi Yamamoto

   Article

   Sakuma et al Nat Protoc. 2016 Jan;11(1):118-33. doi:

   Insert

   humanized S. pyogenes Cas9
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Promoter

   CBh

   Available Since

   Dec. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

2. MSCV-pU6-(BbsI)-CcdB-(BbsI)-Pgk-Puro-T2A-BFP

   Plasmid

   #86457

   Purpose

   Mouse stem cell retroviral vector including a puromycin resistance and BFP gene. sgRNA targets can be cloned in between the BbsI sites

   Depositor

   Ralf Kuehn

   Article

   Chu et al Proc Natl Acad Sci U S A. 2016 Nov 1;113

   Insert

   BFP
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   Pgk

   Available Since

   Feb. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3 Flag beta-1-adrenergic-receptor

   Plasmid

   #14698

   Depositor

   Robert Lefkowitz

   Article

   Tang et al Proc Natl Acad Sci U S A. 1999 Oct 26. 9

   Insert

   B1AR (ADRB1 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Available Since

   June 19, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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4. LentiGuide Puro-P2A-EGFP

   Plasmid

   #137729

   Purpose

   For simple determination of the multiplicity of infection (MOI) of a lentiviral CRISPR library by checking EGFP expression (still allowing for puro selection).

   Depositor

   Fredrik Wermeling

   Article

   Panda et al Proc Natl Acad Sci U S A. 2020 Jan 24. p

   Type

   Empty backbone

   Use

   CRISPR, Lentiviral, and Synthetic Biology

   Tags

   EGFP

   Expression

   Bacterial and Mammalian

   Available Since

   Feb. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMX229

   Plasmid

   #23002

   Depositor

   Linda Wordeman

   Article

   Stumpff et al Dev Cell. 2008 Feb . 14(2):252-62.

   Insert

   Kif18A (KIF18A Human)
   

   Tags

   6His and EGFP

   Expression

   Mammalian

   Available Since

   Feb. 8, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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6. GFP-PH-FAPP1

   Plasmid

   #161986

   Purpose

   Expresses GFP-tagged PH-FAPP1. PI(4)P biosensor

   Depositor

   David Bryant, Tamas Balla

   Article

   Balla et al Mol Biol Cell. 2005 Mar;16(3):1282-95. d

   Insert

   PH-FAPP1 (PLEKHA3 Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Available Since

   Dec. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFRT/FLAG/HA-DEST QKI

   Plasmid

   #19891

   Depositor

   Thomas Tuschl

   Article

   Landthaler et al RNA. 2008 Dec;14(12):2580-96. doi: 10.12

   Insert

   quaking homolog, KH domain RNA binding (QKI Human)
   

   Tags

   FLAG/HA

   Expression

   Mammalian

   Available Since

   Dec. 31, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV-dCas13-inactive M3nls

   Plasmid

   #157854

   Purpose

   Targeted m6A RNA methylation in mammalian cells, methyltransferase-inactive mutant

   Depositor

   David Liu

   Article

   Wilson et al Nat Biotechnol. 2020 Jun 29. pii: 10.103

   Insert

   dCas13-inactive dCas13-inactive M3nls (METTL3 Cas13b is from Prevotella sp. P5-125; METTL3 is from H. sapiens)
   

   Expression

   Mammalian

   Mutation

   PspCas13b Δ984-1090 H133A; METTL3 D395A

   Available Since

   Aug. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1-mRuby4(1-10)

   Plasmid

   #165143

   Purpose

   Expresses mRuby4(1-10) in mammalian cells

   Depositor

   Daichi Kamiyama

   Article

   Tamura et al Commun Biol. 2021 Feb 26;4(1):257. doi:

   Insert

   mRuby4(1-10)
   

   Expression

   Mammalian

   Available Since

   April 8, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pFRT-TODestFLAGHA_HuC

    Plasmid

    #65756

    Purpose

    expresses FLAGHA-tagged HuC in mammalian cells

    Depositor

    Thomas Tuschl

    Insert

    ELAVL3 (ELAVL3 Human)
    

    Tags

    FLAG HA and V5

    Expression

    Mammalian

    Promoter

    CMV, 2x Tet

    Available Since

    June 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. eMagB-TgRFP-T

    Plasmid

    #162252

    Purpose

    Blue-light dependent heterodimerization system

    Depositor

    Pietro De Camilli

    Article

    Benedetti et al Elife. 2020 Nov 11;9. pii: 63230. doi: 1

    Insert

    eMagB-TgRFP-T
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1+U1snRNA

    Plasmid

    #194202

    Purpose

    Expresses U1 snRNA

    Depositor

    Matthias Leisegang, Ralf Brandes

    Insert

    U1 snRNA (RNU1-1 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. rGluA2 (px330:2-guideCas9)

    Plasmid

    #169439

    Purpose

    Expression of Cas9 and 2 guides

    Depositor

    Richard Huganir

    Article

    Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

    Insert

    spCas9
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    June 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTRIPZ-hDDX5/17

    Plasmid

    #71307

    Purpose

    Inducible shRNA knockdown of both human DDX5 and DDX17 genes

    Depositor

    Allan Brasier

    Article

    Yang et al Mol Cell Proteomics. 2015 Oct;14(10):270

    Insert

    DDX5/17 shRNA (DDX17 Human)
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Promoter

    TetO

    Available Since

    Dec. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.1-ccdB-Nanoluc

    Plasmid

    #87067

    Purpose

    Mammalian Gateway-compatible expression vector backbone with a C-terminal Nanoluc luciferase

    Depositor

    Mikko Taipale

    Type

    Empty backbone

    Tags

    Nanoluc luciferase

    Expression

    Mammalian

    Available Since

    March 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV-DAAO

    Plasmid

    #60344

    Purpose

    Yeast D-Amino acid oxidase from Rhodotorula gracilis, optimized for mammalian expression.

    Depositor

    Vsevolod Belousov

    Article

    Matlashov et al Antioxid Redox Signal. 2014 Mar 1;20(7):

    Insert

    DAAO
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. TRE-UBC-mCD47-T2A-GFP

    Plasmid

    #205449

    Purpose

    lentiviral plasmid for expression of mouse CD47

    Depositor

    Deepta Bhattacharya

    Article

    Pizzato et al Stem Cell Reports. 2023 Dec 22:S2213-671

    Insert

    mCD47 (Cd47 Mouse)
    

    Expression

    Mammalian

    Promoter

    UBC

    Available Since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. FosB

    Plasmid

    #187908

    Purpose

    Expresses FosB in transfected cells

    Depositor

    Jennifer Mitchell

    Article

    Mitchell et al Endocrinology. 2005 Apr;146(4):2048-54.

    Insert

    FosB (Fosb Mouse)
    

    Expression

    Mammalian

    Available Since

    Oct. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSBtet-GH

    Plasmid

    #60498

    Purpose

    SB-transposon with inducible SfiI cloning site for GOI (contains firefly luciferase) and constitutive expression of GFP, rtTA and hygromycin resistance gene

    Depositor

    Eric Kowarz

    Article

    Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b

    Type

    Empty backbone

    Use

    Transposon

    Expression

    Mammalian

    Promoter

    TCE & RPBSA

    Available Since

    Feb. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pFRT-TODestFLAGHA_HuD

    Plasmid

    #65757

    Purpose

    expresses FLAGHA-tagged HuD in mammalian cells

    Depositor

    Thomas Tuschl

    Insert

    ELAVL4 (ELAVL4 Human)
    

    Tags

    FLAG HA and V5

    Expression

    Mammalian

    Promoter

    CMV, 2x Tet

    Available Since

    June 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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