77,958 results

1. pCAG-3xFLAG-TurboID-MCS

   Plasmid

   #186813

   Purpose

   CAG promoter-driven expression of TurboID-fused protein (N terminus)

   Depositor

   Masahito Ikawa

   Article

   Oura et al Sci Rep. 2022 Dec 23;12(1):22198. doi: 1

   Type

   Empty backbone

   Tags

   3xFLAG-TurboID

   Expression

   Mammalian

   Promoter

   CAG promoter

   Available Since

   Jan. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. mActA-FKBP-mCherry

   Plasmid

   #210295

   Purpose

   ActuAtor for FKBP-FRB CID

   Depositor

   Takanari Inoue

   Article

   Nakamura et al Cell Rep. 2023 Sep 2:113089. doi: 10.101

   Insert

   mActA-FKBP-mCherry
   

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAG-hPPP3CA-FLAG

   Plasmid

   #179134

   Purpose

   Expression vector of human protein phosphatase 3, catalytic subunit, alpha isoform (PPP3CA) tagged with FLAG at C-terminus, CAG promoter, rabbit globin poly(A) signal.

   Depositor

   Masahito Ikawa

   Article

   Miyata et al Proc Natl Acad Sci U S A. 2021 Aug 31;11

   Insert

   protein phosphatase 3, catalytic subunit, alpha isoform (PPP3CA Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Available Since

   Jan. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1 HA CFP hNKCC1 WT (NT15-H)

   Plasmid

   #49077

   Purpose

   Expresses human NKCC1 with an N-terminal 3xHA-CFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites. Native hNKCC1 amino acid sequence

   Depositor

   Biff Forbush

   Article

   Somasekharan et al PLoS One. 2013 Dec 5;8(12):e82060. doi:

   Insert

   human NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
   

   Tags

   3x HA and mCerulean

   Expression

   Mammalian

   Mutation

   262 silent mutations from native hNKCC1

   Promoter

   CMV

   Available Since

   Nov. 19, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. pmGFP-ADAR2

   Plasmid

   #117929

   Purpose

   ADAR2 expression plasmid.

   Depositor

   Kumiko Ui-Tei

   Article

   Galipon et al Genes (Basel). 2017 Feb 11;8(2). pii: ge

   Insert

   ADAR2 (ADARB1 Human)
   

   Tags

   myc-GFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. 2X_pX458_pSpCas9(BB)-2A-GFP

   Plasmid

   #172221

   Purpose

   Cas9-2A-GFP expression vector bearing two (one additional) independent sgRNA cassettes.

   Depositor

   Alexander Meissner

   Article

   Wu et al Nat Commun. 2021 Aug 12;12(1):4897. doi:

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   3XFLAG and GFP

   Expression

   Mammalian

   Promoter

   Cbh

   Available Since

   Sept. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3 HA-humanTRAF2 (1084)

   Plasmid

   #66932

   Purpose

   Mammalian Expression of TRAF2

   Depositor

   Michael Karin

   Insert

   TRAF2 (TRAF2 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. ratGlp1-R

   Plasmid

   #14944

   Depositor

   Bernard Thorens

   Article

   Thorens et al Proc Natl Acad Sci U S A. 1992 Sep 15. 8

   Insert

   Glp1-R (Glp1r Rat)
   

   Expression

   Mammalian

   Available Since

   June 7, 2007

   Availability

   Academic Institutions and Nonprofits only

9. pLenti-EML4-ALK variant 3a

   Plasmid

   #183829

   Purpose

   Express EML4-ALK fusion variant 3a (E6a;A20) in mammalian cells

   Depositor

   Aaron Hata

   Article

   Yoda et al Cancer Discov. 2018 Jun;8(6):714-729. do

   Inserts

   ALK Receptor Tyrosine Kinase (ALK Human)
   EMAP Like 4 (EML4 Human)
   

   Use

   Lentiviral

   Tags

   The fusion protein of EML4 exon 1-6a and ALK exon…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLV UBC msTMEM115-3C-3xHA-P2A-eGFP

    Plasmid

    #214997

    Purpose

    Stably express the GolgiTag (GolgiIP handle) in mammalian cells (mouse ORF). eGFP for cell sorting desired expression levels.

    Depositor

    Wade Harper

    Article

    Hollingsworth et al bioRxiv 2024.04.19.590338

    Insert

    TMEM115 (Tmem115 Mouse)
    

    Use

    Lentiviral

    Tags

    3xHA

    Expression

    Mammalian

    Promoter

    UBC

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMK290 (mAID-mClover-Hygro)

    Plasmid

    #72828

    Purpose

    C-terminal tagging with mAID-mClover using CRISPR/Cas9

    Depositor

    Masato Kanemaki

    Article

    Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

    Insert

    mAID-mClover-Hygro
    

    Use

    CRISPR

    Tags

    mAID-mClover

    Expression

    Mammalian

    Available Since

    Feb. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMET7-GAG-EGFP

    Plasmid

    #80605

    Purpose

    Mammalian expression vector that expresses HIV-1 GAG with EGFP fused to it

    Depositor

    Sven Eyckerman

    Article

    Eyckerman et al Nat Commun. 2016 Apr 28;7:11416. doi: 10

    Insert

    EGFP
    

    Expression

    Mammalian

    Promoter

    SRalpha

    Available Since

    Aug. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCS6-SMASh-YFP

    Plasmid

    #68852

    Purpose

    Expresses SMASh tag fused to YFP at N terminus in mammalian cells

    Depositor

    Michael Lin

    Article

    Chung et al Nat Chem Biol. 2015 Jul 27. doi: 10.1038

    Insert

    SMASh-YFP
    

    Tags

    SMASh tag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLX-TRE-dCas9-KRAB-MeCP2-BSD

    Plasmid

    #140690

    Purpose

    Inducible knockdown of gene expression in human cells for pooled scRNA-seq experiments

    Depositor

    Andrea Califano

    Article

    Tan et al bioRxiv 2021.06.28.449297

    Insert

    Cas9m4-KRAB-MeCP2
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Oct. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. non-targeting control gRNA (BRDN0001148862)

    Plasmid

    #80263

    Purpose

    3rd generation lentiviral gRNA plasmid, non-targeting control gRNA

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    Non-targeting control gRNA
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    Aug. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. U6-5'+3' MS2-CircRNA

    Plasmid

    #213752

    Purpose

    For circular RNA-mediated prime editor using U6-5'+3' MS2-CircRNA in HEK293T cells

    Depositor

    Caixia Gao

    Article

    Liang et al Nat Biotechnol. 2024 Jan 10. doi: 10.103

    Insert

    5' ribozyme, 5' ligation sequences, 5' MS2, 3' MS2, 3' ligation sequences, 3' ribozyme
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Feb. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pZR071_SFFV-dCas9-mCherry-KRAB

    Plasmid

    #180264

    Purpose

    Lentiviral expression vector for dCas9-KRAB in mammalian cells

    Depositor

    Alexander Marson

    Article

    Schmidt et al Science. 2022 Feb 4;375(6580):eabj4008.

    Insert

    dCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    KRAB and mCherry

    Expression

    Mammalian

    Mutation

    Nuclease dead Cas9

    Promoter

    SFFV

    Available Since

    Feb. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. MAC_N_STA5A

    Plasmid

    #167799

    Purpose

    Expresses STA5A in mammalian cells

    Depositor

    Markku Varjosalo

    Article

    Goos et al Nat Commun. 2022 Feb 9;13(1):766. doi: 1

    Insert

    STA5A (STAT5A Human)
    

    Tags

    MAC

    Expression

    Mammalian

    Mutation

    no mutations

    Available Since

    Jan. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. piggyBAC-BRD4-Flag

    Plasmid

    #214432

    Purpose

    A piggyBAC based plasmid for the stable expression of BRD4 with a flag tag.

    Depositor

    Shaun Cowley

    Insert

    Brd4 bromodomain containing 4 (Brd4 Mouse)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    EF1

    Available Since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pHAGE-EF1a MICA*009-IRES-ZsGreen

    Plasmid

    #114007

    Purpose

    Induces expression of human MICA allele 009 cDNA

    Depositor

    Kai Wucherpfennig

    Article

    Ferrari de Andrade et al Science. 2018 Mar 30;359(6383):1537-1542

    Insert

    MICA*009 (MICA Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Aug. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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