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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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82,515 results

Showing: 4861 - 4880 of 82515 results

  1. pLVX-EF1alpha-SARS-CoV-2-nsp11-2xStrep-IRES-Puro

    Plasmid
    #141377
    Purpose
    Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus
    Depositor
    Nevan Krogan
    Publication
    Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:
    Insert
    SARS-CoV-2-nsp11 (ORF1ab SARS-CoV-2)
    Use
    Lentiviral
    Tags
    2xStrep
    Expression
    Mammalian
    Mutation
    human codon optimized
    Promoter
    EF1alpha
    Available since
    April 9, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    141377-plasmid-map-sequence-id-288063

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  4. pcDNA3-RAB-EKARev

    Plasmid
    #118474
    Purpose
    ddRFP-based single-color biosensor for monitoring ERK activity.
    Depositor
    Jin Zhang
    Publication
    Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    RAB-EKARev
    Tags
    6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    April 30, 2019
    Availability
    Academic Institutions and Nonprofits only
    118474-plasmid-map-sequence-id-243124

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  5. Halo-TOMM20-N-10

    Plasmid
    #123284
    Purpose
    Localization: Outer Mitochondrial Membrane
    Depositor
    Kevin McGowan
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    TOMM20 (TOMM20 Human)
    Tags
    HaloTag7
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    April 24, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    123284-plasmid-map-sequence-id-242688

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  6. CLYBL-TO-hNIL-BSD-mApple

    Plasmid
    #124230
    Purpose
    Targets the human CLYBL safe harbor locus for integration of dox-inducible NGN2-ISL1-LHX3 for differentiation of iPSCs into lower motor neurons. Contains a floxed BSD-NLS-mApple selection cassette.
    Depositor
    Michael Ward
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    NGN2, ISL1, LHX3 (NEUROG2, ISL1, LHX3 Human)
    Expression
    Mammalian
    Available since
    April 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    124230-plasmid-map-sequence-id-240604

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  9. LLP252 pEF1a-NLS-scFvGCN4-DNMT3a

    Plasmid
    #100941
    Purpose
    Single chain variable fragment antibody GCN4 fused to DNMT3a, binds SunTag, with 3xTy1 tag
    Depositor
    Ryan Lister
    Publication
    Pflueger et al Genome Res. 2018 Jun 15. pii: gr.233049.
    Insert
    scFvGCN4, sfGFP, GB1, DNMT3a catalytic domain (DNMT3A Human, Synthetic)
    Tags
    1xHA and 3xTy1
    Expression
    Mammalian
    Mutation
    D10A, H840A for dCas9
    Available since
    June 21, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    100941-plasmid-map-sequence-id-206116

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  12. AAVS1-idCas9-vpr

    Plasmid
    #89985
    Purpose
    inducible CRISPR-ON system for controllable gene activation; this plasmid is used to insert dCas9-VPR casette in one allele of AAVS1 locus
    Depositor
    Jie Na
    Publication
    Guo et al Protein Cell. 2017 Jan 23. doi: 10.1007/
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dCas9-VPR
    Expression
    Mammalian
    Promoter
    TRE-tight
    Available since
    June 6, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    89985-plasmid-map-sequence-id-180638

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  20. NLS-dCas9-trCIB1

    Plasmid
    #64119
    Purpose
    Photoactivatable transcription system. Expression of genomic anchor probe, containing dCas9 and CIB1
    Depositor
    Moritoshi Sato
    Publication
    Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dCas9-trCIB1 fusion
    Tags
    HA and NLS
    Expression
    Mammalian
    Mutation
    dCas9 (D10A H840A, catalytically inactive), trCIB…
    Promoter
    CMV
    Available since
    April 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    64119-plasmid-map-sequence-id-140078

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Showing: 4861 - 4880 of 82515 results