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syntaxin3-myc-myc-His

Plasmid

#12372

Depositor

Thomas Weimbs

Article

Sharma et al J Cell Biol. 2006 Jun 19. 173(6):937-48.

Insert

Syntaxin3 (STX3 Human)

Tags

2xMyc-His

Expression

Mammalian

Mutation

Insert is cloned into a modified vector

Available Since

Sept. 28, 2006

Availability

Academic Institutions and Nonprofits only

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Rc/CMV cyclin D2 HA

Plasmid

#8950

Depositor

Philip Hinds

Article

Baker et al Cell Cycle. 2005 Feb . 4(2):330-8.

Insert

cyclin D2 (CCND2 Human)

Tags

HA

Expression

Mammalian

Available Since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only
pcDNA3 Paf1

Plasmid

#11059

Depositor

Matthew Meyerson

Article

Rozenblatt-Rosen et al Mol Cell Biol. 2005 Jan . 25(2):612-20.

Insert

Paf1 (PAF1 Human)

Expression

Mammalian

Available Since

Dec. 6, 2005

Availability

Academic Institutions and Nonprofits only

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pcDNA-p160MBP

Plasmid

#41

Depositor

Bruce Spiegelman

Article

Fan et al Genes Dev 2004 Feb 1;18(3):278-89. Epub

Insert

p160 myb binding protein (Mybbp1a Mouse)

Tags

myc

Expression

Mammalian

Available Since

July 21, 2005

Availability

Academic Institutions and Nonprofits only

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FLAG-JAK1

Plasmid

#174572

Purpose

Human FLAG-tagged JAK1 expression (N-term. tag) (CMV prom.)

Depositor

Mathew Garnett

Article

Coelho et al Cancer Cell. 2023 Feb 13;41(2):288-303.e

Insert

JAK1 (JAK1 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 19, 2023

Availability

Academic Institutions and Nonprofits only

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mScarlet-EEA1

Plasmid

#169067

Purpose

Express human EEA1 in mammalian cells

Depositor

Gia Voeltz

Article

Wu et al Dev Cell. 2021 Jan 11;56(1):52-66.e7. do

Insert

EEA1 (EEA1 Human)

Tags

mScarlet

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pDARMO_HUWE1_3xFLAG

Plasmid

#187155

Purpose

mammalian cell expression of HUWE1-3xFLAG

Depositor

Eric Fischer

Article

Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

Insert

HUWE1 (HUWE1 Human)

Tags

3xFLAG

Expression

Mammalian

Available Since

Aug. 16, 2022

Availability

Academic Institutions and Nonprofits only

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AAVS1_Puro_hPGK1_EGFP_Donor

Plasmid

#178088

Purpose

HDR donor plasmid to introduce the EGFP cassette to AAVS1 using AAVS1 T2 sgRNA.

Depositor

Yannick Doyon

Article

Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

Insert

EGFP

Use

CRISPR

Expression

Mammalian

Promoter

Human PGK1

Available Since

Dec. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pGP-pcDNA3.1 Puro-CAG-Voltron2

Plasmid

#172909

Purpose

Mammalian expression of voltage sensor

Depositor

GENIE Project

Article

Abdelfattah et al Neuron. 2023 Mar 28:S0896-6273(23)00205-

Insert

Voltron2

Expression

Mammalian

Mutation

A122D

Promoter

CAG

Available Since

Nov. 10, 2021

Availability

Academic Institutions and Nonprofits only

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AttB _EGFP

Plasmid

#171597

Purpose

Strong EGFP fluorescence from Bxb1 landing pad

Depositor

Kenneth Matreyek

Article

Shukla et al PLoS Pathog. 2021 Jul 16;17(7):e1009715.

Insert

EGFP

Use

Synthetic Biology; Promoterless bxb1 dna recombin…

Expression

Mammalian

Available Since

July 21, 2021

Availability

Academic Institutions and Nonprofits only

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pLJM1-FLAG-GFP-Tmem192

Plasmid

#134630

Purpose

lysosomal marker

Depositor

Roberto Zoncu

Article

Lim et al Nat Cell Biol. 2019 Oct;21(10):1206-1218

Insert

TMEM192 (TMEM192 Human)

Use

Lentiviral

Tags

FLAG and GFP

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV-Sport6-CD63-pHuji

Plasmid

#130902

Purpose

Expression of CD63-pHuji for visualization of multivesicular body-plasma membrane fusion.

Depositor

DM Pegtel

Article

Verweij et al J Cell Biol. 2018 Mar 5;217(3):1129-1142

Insert

CD63-pHuji (CD63 Human, synthetic)

Tags

pHuji

Expression

Mammalian

Mutation

pHuji inserted between Gln-36 and Leu-37 in extra…

Promoter

CMV

Available Since

Oct. 10, 2019

Availability

Academic Institutions and Nonprofits only

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pBT372

Plasmid

#125613

Purpose

Expresses evoCDA1-BE4max-NG in mammalian cells

Depositor

David Liu

Article

Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

Insert

evoCDA1-BE4max-NG

Expression

Mammalian

Mutation

F23S A123V I195F

Available Since

July 25, 2019

Availability

Academic Institutions and Nonprofits only

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AAV SYN flex PSAM4 GlyR IRES EGFP

Plasmid

#119741

Purpose

Cre-dependent chemogenetic inhibitor expression

Depositor

Scott Sternson

Article

Magnus et al Science. 2019 Mar 14. pii: science.aav52

Has Service

AAV5 and AAV9

Insert

PSAM4 GlyR IRES eGFP (CHRNA7 Human, Synthetic)

Use

AAV

Expression

Mammalian

Mutation

L131G, Q139L, Y217F

Promoter

synapsin

Available Since

March 18, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-NORAD

Plasmid

#120383

Purpose

Expression vector for lncRNA NORAD

Depositor

Igor Ulitsky

Article

Tichon et al Nat Commun. 2016 Jul 13;7:12209. doi: 10

Insert

NORAD (NORAD Human)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pLNCX2-mCherry-VPS4A

Plasmid

#115334

Purpose

expresses mCherry-VPS4A in mammalian cells

Depositor

Sanford Simon

Article

Johnson et al Elife. 2018 Jul 4;7. pii: 36221. doi: 10

Insert

VPS4A (VPS4A Human)

Use

Retroviral

Tags

mCherry

Expression

Mammalian

Mutation

silent mutation in position 4050 C to T

Promoter

CMV

Available Since

Sept. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pLHCX-ATF4 mScarlet NLS

Plasmid

#115970

Purpose

ER-stress sensor - ATF4 translation at ORF3 fluorescent reporter with mScarlet-I (retroviral vector backbone)

Depositor

David Andrews

Article

Nougarede et al Assay Drug Dev Technol. 2018 Aug 8. doi:

Insert

activating transcription factor 4 (ATF4 Human, Synthetic)

Use

Retroviral

Tags

HA tag, c-myc NLS, and mScarlet-I

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 25, 2018

Availability

Academic Institutions and Nonprofits only

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pLX317-EGFP

Plasmid

#115439

Purpose

Constitutive lentiviral expression

Depositor

William Hahn

Article

Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

Insert

EGFP

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

EF1a

Available Since

Sept. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pENN.AAV.CamKII 0.4.Cre.SV40

Plasmid

#105558

Purpose

AAV expression of Cre from CamKII promoter

Depositor

James M. Wilson

Has Service

AAV Retrograde, AAV1, AAV5, and AAV9

Insert

Cre

Use

AAV and Cre/Lox

Expression

Mammalian

Promoter

CamKII

Available Since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

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Opto-CRAC version 1

Plasmid

#101245

Purpose

Genetically-encoded calcium actuator; mCherry-tagged LOV2-STIM1 chimera that enables optical control of calcium signals and calcium dependent signaling

Depositor

Yubin Zhou

Article

He et al Elife. 2015 Dec 8;4. pii: e10024. doi: 1

Insert

LOV2 and STIM1 cytosolic fragment

Tags

mCherry

Expression

Bacterial and Mammalian

Promoter

CMV

Available Since

April 30, 2018

Availability

Academic Institutions and Nonprofits only

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