78,045 results

1. pCMV-pro-Il1b-C-Flag

   Plasmid

   #75131

   Purpose

   Expresses C-terminal flag-tagged pro-IL-1β in mammalian cells

   Depositor

   Bruce Beutler

   Article

   Shi et al Nat Immunol. 2016 Mar;17(3):250-8. doi:

   Insert

   interleukin 1 beta (Il1b Mouse)
   

   Tags

   3XFLAG

   Expression

   Mammalian

   Mutation

   (please see depositor comments below)

   Promoter

   CMV

   Available Since

   Sept. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLenti PGK GFP Puro (w509-5)

   Plasmid

   #19070

   Purpose

   3rd gen lentiviral eGFP expression vector, PGK promoter, Puro 

   Depositor

   Eric Campeau, Paul Kaufman

   Article

   Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

   Insert

   enhanced green fluorescent protein
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Aug. 12, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3-FLAG-HA-hYTHDC1

   Plasmid

   #85167

   Purpose

   Expression of FLAG-HA-tagged human YTHDC1

   Depositor

   Samie Jaffrey

   Article

   Patil et al Nature. 2016 Sep 7;537(7620):369-373. do

   Insert

   YTHDC1 (YTHDC1 Human)
   

   Tags

   Flag and Ha

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCH61 (multiAsCas12a-KRAB piggyBac)

   Plasmid

   #217332

   Purpose

   piggyBac expression of multiAsCas12a-KRAB

   Depositor

   Luke Gilbert

   Article

   Hsiung et al Nat Biotechnol. 2024 May 17. doi: 10.103

   Insert

   multiAsCas12a
   

   Use

   CRISPR

   Tags

   6xMycNLS and HA-SV40NLS-XTEN80-KRAB-P2A-TagBFP2

   Expression

   Mammalian

   Mutation

   R1226A/E174R/S542R/K548R

   Promoter

   CAG

   Available Since

   March 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. mKeima-Red-C1

   Plasmid

   #54546

   Purpose

   Localization: C1 Cloning Vector, Excitation: 440, Emission: 620

   Depositor

   Michael Davidson

   Type

   Empty backbone

   Tags

   mKeima-Red

   Expression

   Mammalian

   Available Since

   June 12, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCDNA3-STAT3-Y705F

   Plasmid

   #74434

   Purpose

   Expression of STAT3-Y705F

   Depositor

   Jie Chen

   Article

   Kim et al J Biol Chem. 2009 Dec 18;284(51):35425-3

   Insert

   Signal Transducer And Activator Of Transcription 3 (Acute-Phase Response Factor)
   

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Mutations E16K, Y705F

   Promoter

   CMV

   Available Since

   May 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMK462 (Hygro-P2A-mAB)

   Plasmid

   #214388

   Purpose

   mAID-BromoTag for N-terminal tagging

   Depositor

   Masato Kanemaki

   Article

   Hatoyama et al EMBO Rep. 2024 Sep;25(9):4062-4077. doi:

   Insert

   Hygro-P2A-mAID-BromoTag
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Aug. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTRE-Slow-FT

   Plasmid

   #31915

   Depositor

   Vladislav Verkhusha

   Article

   Subach et al Nat Chem Biol. 2009 Feb;5(2):118-26. Epu

   Insert

   Slow-FT
   

   Expression

   Mammalian

   Mutation

   M18V/E30V/K69R/L84W/A179V compared to mCherry (bu…

   Promoter

   PTight Tet-responsive PminCMVd

   Available Since

   Dec. 7, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLentiCas9-BFP

   Plasmid

   #78545

   Purpose

   Fusion protein Cas9+BFP

   Depositor

   Roderic Guigo, Rory Johnson

   Article

   Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

   Insert

   BFP
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   Fusion protein with Cas9

   Available Since

   July 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. PCMV-intron myc Rab2 S20N

    Plasmid

    #46780

    Purpose

    expression of S20N Rab2a in mammalian cells

    Depositor

    Terry Hébert

    Article

    Dupre et al J Biol Chem. 2006 Nov 10;281(45):34561-7

    Insert

    RAB2 (RAB2A Human)
    

    Tags

    myc

    Expression

    Mammalian

    Mutation

    S20N dominant negative

    Promoter

    CMV

    Available Since

    Nov. 14, 2013

    Availability

    Academic Institutions and Nonprofits only

11. pHCMV_CHIKV_E1E2

    Plasmid

    #207261

    Purpose

    Mammalian expression of Chikungunya virus E1E2 protein

    Depositor

    Feng Zhang

    Article

    Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

    Insert

    CHIKV_E1E2 (CHIKVgp2 Chikungunya virus)
    

    Expression

    Mammalian

    Mutation

    mammalian codon optimization

    Promoter

    CMV promoter

    Available Since

    Nov. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. OTR untagged

    Plasmid

    #208913

    Purpose

    encodes the human oxytocin receptor, without any tags

    Depositor

    Christian Gruber

    Article

    Dekan et al Chem Sci. 2021 Feb 4;12(11):4057-4062. d

    Insert

    OXTR (OXTR Human)
    

    Expression

    Mammalian

    Mutation

    TM5: 5x56, A218, Threonin instead of Alanin

    Available Since

    April 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. mCherry KNL1 wt hardened

    Plasmid

    #45224

    Depositor

    Iain Cheeseman, Michael Lampson

    Article

    Liu et al J Cell Biol. 2010 Mar 22;188(6):809-20.

    Insert

    mCherry KNL1 resistant to KNL1 siRNA (KNL1 Human)
    

    Tags

    TEV S15

    Expression

    Mammalian

    Available Since

    June 10, 2013

    Availability

    Academic Institutions and Nonprofits only

14. hCas9_D10A

    Plasmid

    #41816

    Purpose

    Expresses human codon optimized Cas9 D10A mutant which functions as a nickase for genome engineering

    Depositor

    George Church

    Article

    Mali et al Science. 2013 Feb 15;339(6121):823-6. do

    Insert

    Cas9_D10A
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    human codon-optimized, D10A nickase

    Promoter

    CMV

    Available Since

    Jan. 11, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3-SARS-CoV-2-S-RBD-sfGFP

    Plasmid

    #141184

    Purpose

    Mammalian expression plasmid for RBD of SARS-CoV-2 protein S (spike) fused with sfGFP

    Depositor

    Erik Procko

    Article

    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

    Has Service

    Cloning Grade DNA

    Insert

    S surface glycoprotein [ Severe acute respiratory syndrome coronavirus 2 ] (S SARS coronavirus 2)
    

    Tags

    Signal peptide from influenza HA and Superfolder …

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3-EGFP-Rac1-Q61L

    Plasmid

    #12981

    Depositor

    Gary Bokoch

    Article

    Subauste et al J Biol Chem. 2000 Mar 31. 275(13):9725-3

    Insert

    Rac1 constitutively active (RAC1 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Q61L Constitutively active

    Available Since

    Oct. 27, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV-flag YAP2 5SA

    Plasmid

    #27371

    Depositor

    Kunliang Guan

    Article

    Zhao et al Genes Dev. 2007 Nov 1. 21(21):2747-61.

    Insert

    YAP2 (YAP1 Human)
    

    Tags

    flag

    Expression

    Mammalian

    Mutation

    Serines at positions 61, 109, 127, 128, 131, 163,…

    Available Since

    Feb. 3, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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18. px330_Rosa_sgRNA

    Plasmid

    #97007

    Purpose

    Expresses Cas9 and Rosa26 locus specific sgRNA

    Depositor

    Russell Ray

    Article

    Lusk et al BMC Biol. 2022 Jan 28;20(1):28. doi: 10.

    Insert

    Rosa26 sgRNA
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    March 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. SMARCA2-GFP

    Plasmid

    #65390

    Purpose

    GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

    Depositor

    Kyle Miller

    Article

    Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

    Insert

    SMARCA2 (SMARCA2 Human)
    

    Tags

    EmGFP

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available Since

    Oct. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCI-SEP-GluR1

    Plasmid

    #24000

    Depositor

    Robert Malinow

    Article

    Kopec et al J Neurosci. 2006 Feb 15. 26(7):2000-9.

    Insert

    GluR1 (Gria1 Rat)
    

    Tags

    GluR1 signal peptide (aa1-22) and SEP (supereclip…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 5, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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