78,093 results

1. pECE

   Plasmid

   #26453

   Depositor

   William Rutter

   Article

   Ellis et al Cell. 1986 Jun 6;45(5):721-32.

   Type

   Empty backbone

   Expression

   Mammalian

   Available Since

   Nov. 4, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-EF1a-fDIO-Cre

   Plasmid

   #121675

   Purpose

   Expresses Cre recombinase and HA tag in a Flp dependent fashion (fDIO)

   Depositor

   Esteban Engel, Alexander Nectow

   Article

   Schneeberger et al Cell. 2019 Jun 24. pii: S0092-8674(19)30

   Has Service

   AAV Retrograde, AAV1, AAV5, AAV8, and AAV9

   Insert

   NLS-CRE-HA
   

   Use

   AAV

   Tags

   3xHA and SV40-NLS

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Feb. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. PB-TO-MYOD1-shOct4

   Plasmid

   #182309

   Purpose

   Piggybac Tet-ON plasmid for differentiating hiPSCs into skeletal muscle cells via MYOD1 and shOCT4 expression

   Depositor

   iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

   Inserts

   MYOD1-shOct4 (MYOD1 Human)
   puro-T2A-mycNLS-mTagBFP2
   

   Tags

   T2A-mycNLS-mTagBFP2

   Expression

   Mammalian

   Mutation

   shOct4

   Promoter

   EF1a and TRE3G

   Available Since

   April 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. PB-FLuc+GFPd2

   Plasmid

   #127190

   Purpose

   Piggybac transposon plasmid for expression of CMV promoter firefly luciferase (FLuc) and SV40 destabilized green fluorescent protein (GFPd2)

   Depositor

   Jordan Green

   Insert

   Firefly luciferase
   

   Use

   Luciferase

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. B-gTEMP/pcDNA3

   Plasmid

   #188447

   Purpose

   B-gTEMP for mammalian expression

   Depositor

   Takeharu Nagai

   Article

   Lu et al Nano Lett. 2022 Jul 27;22(14):5698-5707.

   Insert

   B-gTEMP
   

   Expression

   Mammalian

   Available Since

   Oct. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. AAT-PB-CD2APtk

   Plasmid

   #86004

   Purpose

   homology donor for AAT locus with piggyBac-flanked DsRedEx_T2A_puroTK cassette

   Depositor

   Tobias Cantz

   Article

   Eggenschwiler et al Sci Rep. 2016 Dec 2;6:38198. doi: 10.103

   Insert

   DsRedEx_T2A_puro_dTK
   

   Use

   Piggybac exciseable

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Feb. 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. CBIG-RORb

   Plasmid

   #48709

   Purpose

   bicistronic expression of RORb and EGFP

   Depositor

   Jeffrey Macklis

   Insert

   RORb (Rorb Mouse)
   

   Use

   Retroviral

   Expression

   Mammalian

   Promoter

   CMV/beta-actin

   Available Since

   Oct. 17, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCS2-mCherry-Cenexin

   Plasmid

   #186435

   Purpose

   Expresses mCherry Cenexin in mammalian cells and zebrafish embryos

   Depositor

   Heidi Hehnly

   Article

   Aljiboury et al Mol Biol Cell. 2022 Aug 1;33(9):br14. do

   Insert

   Cenexin (ODF2 Human)
   

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   CMV IE94

   Available Since

   Aug. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pINTO-N3::hTET3

   Plasmid

   #136364

   Purpose

   Expresses epitope-tagged human TET3 WT in mammalian cells

   Depositor

   Roberto Bonasio

   Article

   Beck et al Am J Hum Genet. 2020 Jan 3. pii: S0002-9

   Insert

   TET3 (TET3 Human)
   

   Tags

   2xStrep-tagII, FLAG, and HA

   Expression

   Mammalian

   Available Since

   Feb. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHybrid-Electra2

    Plasmid

    #179475

    Purpose

    Expresses Electra2 in bacterial cells and Electra2 and mScarlet in mammalian cells

    Depositor

    Kiryl Piatkevich

    Article

    Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

    Inserts

    Electra2
    mScarlet-I
    

    Expression

    Bacterial and Mammalian

    Available Since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEFIRES-P-mTagBFP-KDEL

    Plasmid

    #87163

    Purpose

    Fluorescent marker of endoplasmic reticulum (ER)

    Depositor

    Elina Ikonen

    Article

    Salo et al EMBO J. 2016 Dec 15;35(24):2699-2716. Ep

    Insert

    mTagBFP-KDEL
    

    Tags

    Bip signal peptide (Homo sapiens) and mTagBFP-KDE…

    Expression

    Mammalian

    Promoter

    EF-1 alpha

    Available Since

    May 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. AAV Ef1a-EGFP-WPRE

    Plasmid

    #135428

    Purpose

    Can be used to express EGFP. Can also be used to create adeno-associated virus for delivery of the EGFP sequence

    Depositor

    Rylan Larsen

    Insert

    EGFP
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Feb. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. GluN2A (px330:2-guideCas9)

    Plasmid

    #169428

    Purpose

    Expression of Cas9 and 2 guides

    Depositor

    Richard Huganir

    Article

    Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

    Insert

    spCas9
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    June 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. PB_TRE_Z18-PDGFR_EF1a_eGFP

    Plasmid

    #186304

    Purpose

    Plasmid for inducible mammalian cell expression of the Z18 helixCAM and constitutive expression of an identifying fluorescent protein (eGFP). Flanked with PiggyBac sites for easy line construction.

    Depositor

    George Church

    Article

    Chao et al Cell. 2022 Aug 23. pii: S0092-8674(22)01

    Inserts

    Z18-PDGFR
    eGFP
    

    Tags

    HA, myc

    Expression

    Mammalian

    Available Since

    Aug. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCAG-EGFP(HiBiT)

    Plasmid

    #162589

    Purpose

    N-terminally HiBiT-tagged EGFP under CAG promoter

    Depositor

    Masaharu Somiya

    Article

    Somiya et al Anal Chem. 2021 Mar 24. doi: 10.1021/acs

    Insert

    EGFP
    

    Tags

    HiBiT

    Expression

    Mammalian

    Promoter

    CAG promoter

    Available Since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. wt dynamin 2 pEGFP

    Plasmid

    #34686

    Depositor

    Sandra Schmid

    Insert

    dynamin 2 (Dnm2 Rat)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    D406G, V664A, H692R and Q758R

    Promoter

    CMV

    Available Since

    Feb. 16, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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17. mCherry-Rab11a-7

    Plasmid

    #55124

    Purpose

    Localization: Ras GTPase, Excitation: 587, Emission: 610

    Depositor

    Michael Davidson

    Insert

    Rab11a (RAB11A Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pKLV2.2-h7SKgRNA5(SapI)-hU6gRNA5(BbsI)-PGKpuroBFP-W

    Plasmid

    #72666

    Purpose

    Lentiviral dual CRISPR gRNA expression vector (human 7SK and human U6 promoters)

    Depositor

    Kosuke Yusa

    Article

    Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

    Insert

    h7SKgRNA cassette, hU6gRNA cassette, PGKpuroBFP cassette, WPRE
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Oct. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pmCherry-ATG5

    Plasmid

    #13095

    Depositor

    Roberta Gottlieb

    Article

    Hamacher-Brady et al Cell Death Differ. 2007 Jan;14(1):146-57

    Insert

    ATG5 (Atg5 Mouse)
    

    Tags

    mCherry

    Expression

    Mammalian

    Available Since

    Nov. 15, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLC242-GFP-metap2

    Plasmid

    #100518

    Purpose

    Expresses GFP tagged metap2 in mammalian cells to make lentivirus

    Depositor

    David Sabatini

    Article

    Gu et al Science. 2017 Nov 10;358(6364):813-818.

    Insert

    Metap2 (METAP2 Human)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    Nov. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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