78,093 results

1. pTfR-mTurquoise2

   Plasmid

   #112953

   Purpose

   In vivo visualization of Transferrin receptors (can be used for colocalization studies)

   Depositor

   Dorus Gadella

   Insert

   pTfR
   

   Tags

   mTurquoise2

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLV-ER GFP

   Plasmid

   #80069

   Purpose

   To express and target GFP into the endoplasmic reticulum using lentivirus

   Depositor

   Pantelis Tsoulfas

   Insert

   ER GFP (SEC61B Human)
   

   Use

   Lentiviral

   Tags

   GFP derived from Aequorea coerulescens on the NH2…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 27, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCDNA3_dCas7-11

   Plasmid

   #197605

   Purpose

   Expresses inactive Cas7-11 in mammalian cells

   Depositor

   Feng Zhang

   Article

   Strecker et al Science. 2022 Nov 25;378(6622):874-881.

   Insert

   dCas7-11
   

   Expression

   Mammalian

   Available Since

   Aug. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pT7-NSP2SA11

   Plasmid

   #89169

   Purpose

   Express Rotavirus SA11 NSP2 in mammalian cells

   Depositor

   Takeshi Kobayashi

   Article

   Kanai et al Proc Natl Acad Sci U S A. 2017 Jan 30. p

   Insert

   Rotavirus SA11 NSP2
   

   Expression

   Mammalian

   Promoter

   T7 promotor

   Available Since

   April 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. dCas9-GCN5 core

   Plasmid

   #179551

   Purpose

   encodes S. pyogenes dCas9 with c-terminal fusion of human GCN5 core (aa 473-676) driven by EF-1alpha promoter

   Depositor

   Isaac Hilton

   Article

   Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78

   Insert

   S. pyogenes dCas9 with c-terminal fusion of human GCN5 core (aa 473-676) (KAT2A Human)
   

   Use

   Lentiviral

   Tags

   Flag Tag

   Expression

   Mammalian

   Mutation

   D10A; H840A in S.pyogenes Cas9

   Promoter

   EF-1alpha

   Available Since

   Feb. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. HA-APP-mGFP

   Plasmid

   #196696

   Purpose

   Expresses APP695 with an HA tag an N-terminal for single molecule tracking and other measusers in mammalian cells

   Depositor

   Martino Calamai

   Article

   Capitini et al iScience. 2023 Apr 11;26(5):106611. doi:

   Insert

   Amyloid Precursor Protein 695 isoform (APP Human)
   

   Tags

   HA and mGFP

   Expression

   Mammalian

   Available Since

   April 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. LifeAct-HaloTag

   Plasmid

   #176105

   Purpose

   F-actin marker

   Depositor

   Dorus Gadella

   Insert

   LifeAct
   

   Expression

   Mammalian

   Available Since

   Oct. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1-FGFR1c-V5/HIS

   Plasmid

   #201106

   Purpose

   expression of human FGFR1 receptor tyrosine kinase in mammalian cells

   Depositor

   Pavel Krejčí

   Article

   Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

   Insert

   human FGFR1 receptor tyrosine kinase, variant c, full length, wildtype (FGFR1 Human)
   

   Tags

   V5/His

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHR-mCh-Cry2olig

   Plasmid

   #101222

   Purpose

   Cry2olig(E490G)

   Depositor

   Clifford Brangwynne

   Article

   Shin et al Cell. 2017 Jan 12;168(1-2):159-171.e14.

   Insert

   Cry2olig
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   E490G

   Promoter

   SFFV

   Available Since

   Feb. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. SETD2-GFP

    Plasmid

    #80653

    Purpose

    mammalian expression of SETD2-GFP

    Depositor

    Sérgio De Almeida

    Article

    Carvalho et al Elife. 2014 May 6;3:e02482. doi: 10.7554

    Insert

    SETD2 (SETD2 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. Flag-JunWT-Myc

    Plasmid

    #47443

    Purpose

    mammalian expression of WT c-Jun

    Depositor

    Axel Behrens

    Article

    Aguilera et al Nature. 2011 Jan 13;469(7329):231-5. doi

    Insert

    Jun (Jun Mouse)
    

    Tags

    Flag and myc

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 28, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. Tubulin-mGreenLantern

    Plasmid

    #164460

    Purpose

    Localization of mGreenLantern fluorescent protein to tubulin

    Depositor

    Gregory Petsko

    Article

    Campbell et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

    Insert

    mGreenLantern
    

    Tags

    Tubulin

    Expression

    Mammalian

    Mutation

    Clover-F64L/S72A/E124V/N149K/I167T/S175G/A206K/L2…

    Promoter

    CMV

    Available Since

    Feb. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLNHA-C1-Hs4E-BP1

    Plasmid

    #79438

    Purpose

    Expresses lambdaN-HA-tagged human 4E-BP1 in Mammalian cells

    Depositor

    Elisa Izaurralde

    Article

    Peter et al Mol Cell. 2015 Mar 19;57(6):1074-87. doi

    Insert

    Hs4E-BP1 (EIF4EBP1 Human)
    

    Tags

    lambdaN-HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. ATG16L1 sgRNA

    Plasmid

    #207563

    Purpose

    pX330 expressing Cas9 and a sgRNA targeting the ATG16L1 locus

    Depositor

    Jens Schmidt

    Article

    Broadbent et al J Cell Biol. 2023 Jul 3;222(7):e20221007

    Insert

    GCAGCAAGTGACATGTCGTC
    

    Expression

    Mammalian

    Promoter

    CMV and U6

    Available Since

    March 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBABE-puro-GFP-progerin

    Plasmid

    #17663

    Depositor

    Tom Misteli

    Article

    Scaffidi et al Nat Cell Biol. 2008 Apr;10(4):452-9. doi

    Insert

    GFP-D50 lamin A (LMNA Human)
    

    Use

    Retroviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Delta 50

    Available Since

    March 27, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLVX-EF1a-EGFP-RAB11B-IRES-Puromycin

    Plasmid

    #134860

    Purpose

    Expresses EGFP-tagged recycling endosome marker

    Depositor

    David Andrews

    Article

    Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

    Insert

    RAB11B (RAB11B Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    March 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pmirGLO-3'UTR mouse Tnf

    Plasmid

    #207127

    Purpose

    Luciferase vector containing 3'UTR for mouse Tnf

    Depositor

    Silvia Monticelli

    Article

    Leoni et al Nat Commun. 2023 Jun 29;14(1):3862. doi:

    Insert

    Tumor necrosis factor (Tnf) 3'UTR (Tnf Mouse)
    

    Use

    Luciferase

    Expression

    Mammalian

    Promoter

    PGK promoter

    Available Since

    Dec. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. lentiCRISPR - EGFP sgRNA 1

    Plasmid

    #51760

    Purpose

    Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an EGFP targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.

    Depositor

    Feng Zhang

    Article

    Shalem et al Science. 2014 Jan 3;343(6166):84-7. doi:

    Inserts

    Cas9
    Puromycin resistance
    EGFP sgRNA 1
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    EFS and U6

    Available Since

    March 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. 9ao-SOD2-3xHA-TALE(ND4-Left)-Nt.BspD6I(C)

    Plasmid

    #209293

    Purpose

    mitoBE expression vector, target mtND4 gene.

    Depositor

    Wensheng Wei

    Article

    Yi et al Nat Biotechnol. 2023 May 22. doi: 10.103

    Insert

    SOD2-3xHA-TALE(ND4-Left)-Nt.BspD6I(C)
    

    Expression

    Mammalian

    Available Since

    Nov. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. GPRC5A-Tango

    Plasmid

    #66382

    Purpose

    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

    Depositor

    Bryan Roth

    Article

    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

    Insert

    GPRC5A (GPRC5A Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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