78,128 results

1. CDK9 gRNA (BRDN0001162576)

   Plasmid

   #77170

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human CDK9

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   CDK9 (CDK9 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMK449 (BromoTag-mClover-BSD)

   Plasmid

   #214379

   Purpose

   BromoTag-mClover for C-terminal tagging

   Depositor

   Masato Kanemaki

   Article

   Hatoyama et al EMBO Rep. 2024 Sep;25(9):4062-4077. doi:

   Insert

   BromoTag-mClover-BSD
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Aug. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMK464 (mAB-Neo)

   Plasmid

   #214390

   Purpose

   mAID-BromoTag for C-terminal tagging

   Depositor

   Masato Kanemaki

   Article

   Hatoyama et al EMBO Rep. 2024 Sep;25(9):4062-4077. doi:

   Insert

   mAID-BromoTag-Neo
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Aug. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLenti6/UbC/mSlc7a1

   Plasmid

   #17224

   Depositor

   Shinya Yamanaka

   Article

   Takahashi et al Cell. 2007 Nov 30. 131(5):861-72.

   Insert

   Slc7a1 (Slc7a1 Mouse)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Feb. 8, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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5. LncRNA overexpression EF1α_BbsI_SV40 polyA_EGFP

   Plasmid

   #219828

   Purpose

   Overexpression vector for non-coding RNAs driven by EF1α promoter with GFP. Cloning using BbsI.

   Depositor

   Philipp Maass

   Type

   Empty backbone

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   July 31, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. TFORF3448

   Plasmid

   #144924

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   TP63 (TP63 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Aug. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. Flag-neo-PP1-Beta

   Plasmid

   #171269

   Purpose

   Mammalian expression of 3x-Flag-tagged human PP1 beta catalytic subunit

   Depositor

   Steven Balk

   Insert

   3x-Flag-tagged human PP1 beta catalytic subunit (PPP1CB Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV enAsCas12f-YHAM-sgRNA_v7

   Plasmid

   #208356

   Purpose

   Expression of enAsCas12f-YHAM and sgRNA_v7 in mammalian cells

   Depositor

   Atsushi Hoshino

   Article

   Hino et al Cell. 2023 Sep 22:S0092-8674(23)00963-7.

   Insert

   enAsCas12f-YHAM
   

   Expression

   Mammalian

   Mutation

   F48Y/S188H/V232A/E316M

   Promoter

   CMV

   Available Since

   Oct. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLVX-opto-mMLKL

   Plasmid

   #208784

   Purpose

   Inducibly expresses mouse MLKL fused to light-activatable Cry2

   Depositor

   Petr Broz

   Article

   Shkarina et al J Cell Biol. 2022 Jun 6;221(6). pii: 213

   Insert

   mCherry mMLKL Cry2 (Mlkl Mouse)
   

   Use

   Lentiviral

   Tags

   Cry2 and mCherry

   Expression

   Mammalian

   Available Since

   Nov. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHCMV-SS-antiHAscFv-TM

    Plasmid

    #198302

    Purpose

    Displays anti-HA scFv from the surface of mammalian cells

    Depositor

    Feng Zhang

    Article

    Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

    Insert

    pHCMV-SS-antiHAscFv-TM
    

    Expression

    Mammalian

    Available Since

    March 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. MTRAP-bio

    Plasmid

    #47746

    Purpose

    Expresses enzymatically monobiotinylated full-length MTRAP ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.

    Depositor

    Gavin Wright

    Article

    Crosnier et al Mol Cell Proteomics. 2013 Sep 16.

    Insert

    Codon-optimised MTRAP
    

    Tags

    enzymatic biotinylation sequence and rat Cd4 d3+4

    Expression

    Mammalian

    Mutation

    Exogenous signal peptide of mouse origin, changed…

    Promoter

    CMV

    Available Since

    Feb. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. Str-Ii_neomycin

    Plasmid

    #65312

    Purpose

    Cytoplasmic ER hook only (RUSH system)

    Depositor

    Franck Perez

    Article

    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

    Insert

    Invariant chain (p33)
    

    Expression

    Mammalian

    Mutation

    Streptavidin in its cytoplasmid part

    Promoter

    CMV

    Available Since

    June 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pdCas9-GFP

    Plasmid

    #181906

    Purpose

    Fluorescently tagged dead Cas9

    Depositor

    Karsten Rippe

    Article

    Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

    Insert

    dCas9
    

    Use

    CRISPR

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pFH2.32_LV-mCherry-MS2x8-BCS

    Plasmid

    #205539

    Purpose

    Express mCherry-MS2x8 with barcode

    Depositor

    Michael Elowitz

    Article

    Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

    Insert

    mCherry
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    EF1

    Available Since

    Sept. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA 3.2/V5-DEST MTFR1L-V5

    Plasmid

    #69822

    Purpose

    Expresses MTFR1L-V5 in mammalian cells

    Depositor

    Anne Brunet

    Article

    Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

    Insert

    Mitochondrial fission regulator 1-like (MTFR1L Human)
    

    Use

    Retroviral

    Tags

    v5

    Expression

    Mammalian

    Promoter

    cmv

    Available Since

    April 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLVX-EF1alpha-SARS-CoV-2-M-2xStrep-IRES-Puro

    Plasmid

    #141386

    Purpose

    Lentiviral expression of SARS-CoV-2 membrane (M) protein; transient expression and generate lentivirus

    Depositor

    Nevan Krogan

    Article

    Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

    Insert

    SARS-CoV-2-M (M SARS-CoV-2)
    

    Use

    Lentiviral

    Tags

    2xStrep

    Expression

    Mammalian

    Mutation

    human codon optimized

    Promoter

    EF1alpha

    Available Since

    April 8, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. pcDNA3-EGFP-Cdc42(wt)

    Plasmid

    #12599

    Depositor

    Klaus Hahn

    Article

    Nalbant et al Science. 2004 Sep 10. 305(5690):1615-9.

    Insert

    EGFP-Cdc42 (WT) (CDC42 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    Oct. 16, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAVS1-NC-CRISPRi (Gen3)

    Plasmid

    #73499

    Purpose

    Constitutive CRISPR interference (CRISPRi) knock in construct into the AAVS1 locus

    Depositor

    Bruce Conklin

    Article

    Mandegar et al Cell Stem Cell. 2016 Apr 7;18(4):541-53.

    Insert

    dCas9-KRAB
    

    Use

    CRISPR

    Tags

    HA, KRAB, and NLS

    Expression

    Mammalian

    Mutation

    D10A, H840A (catalytically deactivated Cas9)

    Promoter

    CAG

    Available Since

    Dec. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pIRES2_EGFP_KCNQ1

    Plasmid

    #173161

    Purpose

    For mammalian expression of KCNQ1 and creating variants

    Depositor

    Al George

    Article

    Vanoye et al Circ Genom Precis Med. 2018 Nov;11(11):e

    Insert

    KCNQ1 (KCNQ1 Human)
    

    Tags

    IRES2-EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. Beta-arrestin2 GFP WT

    Plasmid

    #35411

    Depositor

    Robert Lefkowitz

    Article

    Shenoy et al J Biol Chem. 2005 Apr 15;280(15):15315-2

    Insert

    βarrestin2 (Arrb2 Rat)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 16, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge