79,308 results

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px330_Rosa_sgRNA

Plasmid

#97007

Purpose

Expresses Cas9 and Rosa26 locus specific sgRNA

Depositor

Russell Ray

Article

Lusk et al BMC Biol. 2022 Jan 28;20(1):28. doi: 10.

Insert

Rosa26 sgRNA

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

March 3, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG2-EGFP-C-IFT139

Plasmid

#218738

Purpose

Expresses C-terminally EGFP-tagged IFT139 in mammalian cells

Depositor

Kazuhisa Nakayama

Insert

IFT139 (TTC21B Human)

Tags

EGFP

Expression

Mammalian

Promoter

CAG

Available Since

May 22, 2024

Availability

Academic Institutions and Nonprofits only

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pLC-ZsGreen-P2A-Puro

Plasmid

#124302

Purpose

Lentiviral vector for expression of ZsGreen-P2A-Puro casette from a CMV promoter

Depositor

Eva Gottwein

Article

Patil et al Blood Adv. 2019 Jul 23;3(14):2105-2117.

Insert

ZsGreen

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

July 22, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3-LacR-TopBP1

Plasmid

#31317

Depositor

Aziz Sancar

Article

Lindsey-Boltz et al J Biol Chem. 2011 Jun 3. 286(22):19229-3

Insert

TopBP1 (TOPBP1 Human)

Tags

FLAG and LacR

Expression

Mammalian

Available Since

Nov. 9, 2011

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG-HA-hYTHDC1

Plasmid

#85167

Purpose

Expression of FLAG-HA-tagged human YTHDC1

Depositor

Samie Jaffrey

Article

Patil et al Nature. 2016 Sep 7;537(7620):369-373. do

Insert

YTHDC1 (YTHDC1 Human)

Tags

Flag and Ha

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 20, 2016

Availability

Academic Institutions and Nonprofits only

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pFLAG-HHARI

Plasmid

#17450

Depositor

Dong-Er Zhang

Article

Okumura et al Genes Dev. 2007 Feb 1. 21(3):255-60.

Insert

HHARI (ARIH1 Human)

Tags

Flag

Expression

Mammalian

Available Since

March 5, 2008

Availability

Academic Institutions and Nonprofits only

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pCDNA-3xFLAG-Pontin

Plasmid

#51635

Purpose

Mammalian expression of 3xFLAG tagged pontin, N-terminal.

Depositor

Steven Artandi

Article

Venteicher et al Cell. 2008 Mar 21;132(6):945-57. doi: 10

Insert

Pontin (RUVBL1 Human)

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

May 16, 2014

Availability

Academic Institutions and Nonprofits only

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CRISPR_HLA_class_I_1

Plasmid

#164986

Purpose

Expression of gRNA targeting multiple HLA-A, HLA-B, and HLA-C genes

Depositor

Matthew Meyerson

Article

Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

Insert

gRNA against HLA class I

Use

CRISPR

Expression

Mammalian

Available Since

March 23, 2021

Availability

Academic Institutions and Nonprofits only

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pUltra-Chili-Luc

Plasmid

#48688

Purpose

3rd generation Lentiviral vector with bi-cistronic expression of dtomato and luciferase

Depositor

Malcolm Moore

Article

Fujiwara et al Mol Cancer Ther. 2021 Jun 4. pii: 1535-7

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

June 16, 2014

Availability

Academic Institutions and Nonprofits only

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GluN2B (px330:2-guideCas9)

Plasmid

#169430

Purpose

Expression of Cas9 and 2 guides

Depositor

Richard Huganir

Article

Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

Insert

spCas9

Use

CRISPR

Expression

Mammalian

Available Since

June 21, 2021

Availability

Academic Institutions and Nonprofits only

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pDEST-FRT/T0-Flag-PALB2

Plasmid

#71114

Purpose

mammalian expession of FLAG-PALB2

Depositor

Daniel Durocher

Article

Orthwein et al Nature. 2015 Dec 9. doi: 10.1038/nature1

Insert

PALB2 (PALB2 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV/TO

Available Since

Dec. 9, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pUC CBA-SpCas9.EF1a-BFP.sgLMNA

Plasmid

#98971

Purpose

Cas9 Homologous Recombination Reporter. SpCas9 and a sgRNA targeting LMNA. TagBFP.

Depositor

Jan Karlseder

Article

Arnoult et al Nature. 2017 Sep 20;549(7673):548-552. d

Insert

LMNA sgRNA and spCas9

Expression

Mammalian

Available Since

Dec. 11, 2017

Availability

Academic Institutions and Nonprofits only

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mRFP1-N1

Plasmid

#54635

Purpose

Localization: N1 Cloning Vector, Excitation: 584, Emission: 607

Depositor

Robert Campbell, Michael Davidson, Roger Tsien

Article

Campbell et al Proc Natl Acad Sci U S A. 2002 Jun 11;99

Type

Empty backbone

Tags

mRFP1

Expression

Mammalian

Available Since

June 11, 2014

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsDCP1a

Plasmid

#25030

Depositor

Elisa Izaurralde

Article

Tritschler et al Proc Natl Acad Sci U S A. 2009 Dec 22. 1

Insert

DCP1a (DCP1A Human)

Tags

EGFP

Expression

Mammalian

Available Since

June 23, 2010

Availability

Academic Institutions and Nonprofits only

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pCAGGS Donor mClover-LMNA

Plasmid

#98970

Purpose

Donor for endogenous N-ter tagging of hLMNA with mClover. Cas9 Homologous Recombination Reporter

Depositor

Jan Karlseder

Article

Arnoult et al Nature. 2017 Sep 20;549(7673):548-552. d

Insert

LMNA5'UTR-mClover-LMNA5'exon1 (LMNA Human)

Expression

Mammalian

Available Since

Nov. 29, 2017

Availability

Academic Institutions and Nonprofits only

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Lenti-dual-CRISPR-U6-H1(pBP43)

Plasmid

#218930

Purpose

The backbone lentiviral dual-CRISPR plasmid for making the dual-CRISPR screening libraries.

Depositor

Baoxu Pang

Article

Li et al Nat Biomed Eng. 2024 May 22. doi: 10.103

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

human U6 and H1

Available Since

July 17, 2024

Availability

Academic Institutions and Nonprofits only

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AAV-hGFAP-mCherry

Plasmid

#178581

Purpose

Expression of mCherry under the human GFAP promoter

Depositor

Chun-Li Zhang

Article

Wang et al Cell. 2021 Oct 14;184(21):5465-5481.e16.

Insert

mCherry

Use

AAV

Expression

Mammalian

Available Since

Feb. 8, 2022

Availability

Academic Institutions and Nonprofits only

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GFP-UBR5

Plasmid

#52050

Purpose

CMV driven mammalian expression of UBR5 with an N-termianl GFP fusion

Depositor

Darren Saunders

Article

Gudjonsson et al Cell. 2012 Aug 17;150(4):697-709. doi: 1

Insert

UBR5 (UBR5 Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

March 24, 2014

Availability

Academic Institutions and Nonprofits only

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Green Glifon600

Plasmid

#126207

Purpose

green fluorescent glucose sensor for mammalian cells

Depositor

Tetsuya Kitaguchi

Article

Mita et al Anal Chem. 2019 Apr 2;91(7):4821-4830. d

Insert

Green Glifon600

Expression

Mammalian

Available Since

June 7, 2019

Availability

Academic Institutions and Nonprofits only

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pClneoMyc-LATS1

Plasmid

#66851

Purpose

Express Myc-tagged LATS1 in mammalian cells

Depositor

Yutaka Hata

Article

Bao et al J Biochem. 2011 Aug;150(2):199-208. doi:

Insert

LATS1 (LATS1 Human)

Tags

Myc

Expression

Mammalian

Mutation

P44L mutation in LATS1 compared to GenBank refere…

Promoter

CMV

Available Since

Dec. 21, 2015

Availability

Academic Institutions and Nonprofits only

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