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81,861 results

Showing: 9401 - 9420 of 81861 results

  1. 1.3xWT HBV-Luciferase

    Plasmid
    #61549
    Purpose
    The 1.3xHBV-Luciferase plasmid to monitor HBV transcription. 1. Luciferase inserted within Core ORF. 2. Luciferase is regulated by EnhII - Core promoter. 3. The functional luciferase ATG is of Core
    Depositor
    Yosef Shaul
    Article
    Doitsh et al Mol Cell Biol. 2004 Feb;24(4):1799-808.
    Insert
    1.3 times of the HBV genome with luciferase within the Core ORF
    Use
    Luciferase
    Expression
    Mammalian
    Mutation
    Substitution of HBV sequences with luciferase ORF…
    Available Since
    June 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    61549-plasmid-map-sequence-id-212455

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  4. eMscL G22S

    Plasmid
    #107455
    Purpose
    eMscL G22S is optimized for mammalian rodent neuronal expression to the plasma membrane through a neuron-specific promoter and a voltage-gated channel targeting motif
    Depositor
    Francesco Difato
    Article
    Soloperto et al J Cell Sci. 2018 Jan 29. pii: jcs.210393
    Insert
    bacterial mechanosensitive ion channel of large conductance (mscL Escherichia coli)
    Use
    Adeno-associated viral
    Tags
    Kir2.1 ER export signal (FCYENEV) and tdTomato fl…
    Expression
    Mammalian
    Mutation
    Bearing a glycine to serine substitution at posit…
    Promoter
    human synapsin 1
    Available Since
    April 4, 2018
    Availability
    Academic Institutions and Nonprofits only
    107455-plasmid-map-sequence-id-207549

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  7. pKK-BI16-ORF1-3C-mRuby3_ORF2-TEV-mClover3

    Plasmid
    #105802
    Purpose
    Concomitant expression of two proteins. One protein expressed with mRuby3, cleavable by 3C; second protein expressed with mClover3, cleavable by TEV protease; tags positions: C-termini.
    Depositor
    Andrzej Dziembowski
    Article
    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do
    Type
    Empty backbone
    Use
    Flp-in competent
    Tags
    ORF1: 3C-mRuby3; ORF2: TEV-mClover3
    Expression
    Mammalian
    Available Since
    Feb. 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    105802-plasmid-map-sequence-id-203396

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  12. Ezh2[SET]-dCas9

    Plasmid
    #100087
    Purpose
    Catalytic domain [SET] of mouse Ezh2 fused to N-terminus of dCas9; pCDNA3 vector backbone, mammalian expression
    Depositor
    David Segal
    Article
    O'Geen et al Nucleic Acids Res. 2017 Sep 29;45(17):99
    Insert
    Ezh2[SET] (Ezh2 Mouse)
    Use
    CRISPR
    Tags
    3XFLag-NLS-Ezh2[SET]-dCas9-NLS
    Expression
    Mammalian
    Mutation
    Ezh2[SET] includes aa 482-746
    Promoter
    CMV
    Available Since
    Oct. 30, 2017
    Availability
    Academic Institutions and Nonprofits only
    100087-plasmid-map-sequence-id-197556

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  15. pLenti-Mito-Car-GECO

    Plasmid
    #100765
    Purpose
    Lentiviral tet-inducible expression of mito-targeted genetically encoded Ca2+-indicators for optical imaging
    Depositor
    Joseph Gordon
    Article
    Mughal et al Cell Death Differ. 2018 Mar 6. pii: 10.1
    Insert
    Lenti-Mito-Car-GECO
    Use
    Lentiviral
    Tags
    a duplex of the mitochondrial targeting signal of…
    Expression
    Mammalian
    Mutation
    R-GECO1: E163V/I166V/V174T/M176I/F222I/A302P
    Promoter
    CMV
    Available Since
    Sept. 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    100765-plasmid-map-sequence-id-193070

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  16. pCRISPR-S12

    Plasmid
    #84031
    Purpose
    To episomally express codon optimized Cas9 and chimeric guide RNA
    Depositor
    Sen Wu
    Article
    Li et al Protein Cell. 2016 Sep;7(9):689-91. doi:
    Inserts
    hSpCas9
    eGFP
    Tags
    3x FLAG
    Expression
    Mammalian
    Promoter
    CMV and CMV (downstream of F2A self-cleaving pept…
    Available Since
    Aug. 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    84031-plasmid-map-sequence-id-192362

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Showing: 9401 - 9420 of 81861 results