78,128 results

1. Actin-cpstFRET-Actin

   Plasmid

   #80643

   Purpose

   cpstFRET actin sensor for mechanical force measurements in cytoskeleton

   Depositor

   Fred Sachs

   Article

   Guo et al Proc Natl Acad Sci U S A. 2014 Dec 9;111

   Insert

   Beta actin, cpstFRET
   

   Expression

   Mammalian

   Available Since

   Aug. 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pAAV-hSyn-FLEX-rc [Jaws-KGC-GFP-ER2]

   Plasmid

   #78174

   Purpose

   AAV-mediated expression of Jaws-KGC-GFP-ER2 under the human synapsin promoter, in floxed/reversed (Cre-dependent) manner.

   Depositor

   Edward Boyden

   Article

   Chuong et al Nat Neurosci. 2014 Aug;17(8):1123-9. doi

   Insert

   Jaws-KGC-GFP-ER2
   

   Use

   AAV

   Tags

   ER2, GFP, and KGC

   Expression

   Mammalian

   Mutation

   K200R W214F

   Promoter

   human synapsin

   Available Since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. p663-UBC-miniTurbo-V5-CDK20_IDG-K

   Plasmid

   #189883

   Purpose

   Gateway destination clone of CDK20 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

   Depositor

   Ben Major

   Insert

   CDK20 (CDK20 Human)
   

   Use

   Lentiviral; Gateway destination

   Tags

   miniTurbo-V5

   Expression

   Mammalian

   Promoter

   Ubiquitin

   Available Since

   Oct. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pcDNA3-Flag-Tet2 CD

   Plasmid

   #72219

   Purpose

   Express flag-tagged mouse Tet2 catalytic domain.

   Depositor

   Yi Zhang

   Article

   Ito et al Science. 2011 Sep 2;333(6047):1300-3. do

   Insert

   Ten-Eleven Translocation 2 (Tet2 Mouse)
   

   Tags

   flag

   Expression

   Mammalian

   Mutation

   The plasmid expresses TET2 catalytic domain.

   Available Since

   May 1, 2018

   Availability

   Academic Institutions and Nonprofits only

5. pCAG PSAM4 5HT3 LC IRES GFP

   Plasmid

   #123066

   Purpose

   Chemogenetic activator expression plasmid

   Depositor

   Scott Sternson

   Article

   Magnus et al Science. 2019 Mar 14. pii: science.aav52

   Insert

   PSAM4 5HT3 LC IRES GFP (Htr3a Mouse)
   

   Expression

   Mammalian

   Mutation

   L131G Q139L Y217F

   Promoter

   CAG

   Available Since

   April 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. CROP-seq-opti-dsRed

   Plasmid

   #201999

   Purpose

   Based on CROP-seq-opti (addgene #106280), we replaced the PURO resistance gene with a dsRed.

   Depositor

   Daniel Geschwind

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   dsRed

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   June 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pMSSM110: pCMV-HA-NLS-SpuFz1-v2

   Plasmid

   #205277

   Purpose

   Human expression vector for human codon optimized N-terminal NLS-tagged SpuFz1-v2

   Depositor

   Feng Zhang

   Article

   Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

   Insert

   SpuFz1
   

   Tags

   HA, NLS(Makkerh)

   Expression

   Mammalian

   Mutation

   C310R/D487K/T513K

   Available Since

   Aug. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. ALPK1 gRNA (BRDN0001146035)

   Plasmid

   #76081

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human ALPK1

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   ALPK1 (ALPK1 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. ALPK1 gRNA (BRDN0001145294)

   Plasmid

   #76083

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human ALPK1

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   ALPK1 (ALPK1 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. ALPK1 gRNA (BRDN0001146875)

    Plasmid

    #76084

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human ALPK1

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    ALPK1 (ALPK1 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA5/FRT/TO_H2B-HaloTag7-T2A-Tom20-HaloTag9

    Plasmid

    #175547

    Purpose

    Co-expression of HaloTag7 (nuclear) and 9 (mitochondrial surface) in mammalian cells

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

    Insert

    H2B-HaloTag7 and Tomm20-HaloTag9
    

    Expression

    Mammalian

    Mutation

    HaloTag9 = HaloTag7-Q165H-P174R

    Available Since

    Dec. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. FCGR2a-bio-His

    Plasmid

    #51754

    Purpose

    Expresses full-length Low affinity immunoglobulin gamma Fc region receptor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    FCGR2a (FCGR2A Human)
    

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pFUGW-H1 empty vector

    Plasmid

    #25870

    Purpose

    3rd generation lentiviral vector

    Depositor

    Sally Temple

    Article

    Fasano et al Cell Stem Cell. 2007 Jun 7. 1(1):87-99.

    Type

    Empty backbone

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    H1

    Available Since

    Aug. 4, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. mito-mScarlet

    Plasmid

    #141153

    Purpose

    Fluorescent labeling of the mitochondrial matrix in mammalian cells

    Depositor

    Gia Voeltz

    Article

    Abrisch et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

    Insert

    COX4 (COX4 Budding Yeast)
    

    Tags

    mScarlet

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. GLUT1-eGFP/pcDNA-DEST47

    Plasmid

    #18729

    Depositor

    Wolf Frommer

    Article

    Takanaga et al Biochim Biophys Acta. 2008 Apr . 1778(4)

    Insert

    pcDNA-DEST47 /hGLUT1-eGFP
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    deleted TAA

    Available Since

    July 3, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Lyso-B

    Plasmid

    #209873

    Purpose

    Dimerization dependent fluorescent protein B anchored to cytosolic face of the lysosomal membrane with human LAMP1

    Depositor

    Sarah Cohen

    Article

    Miner et al Contact (Thousand Oaks). 2024 Feb 4;7:25

    Insert

    ddGFP B
    

    Tags

    LAMP1

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. TARDBP

    Plasmid

    #156073

    Purpose

    For use in RBP tethering screen

    Depositor

    Eugene Yeo

    Article

    Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

    Insert

    TARDBP (TARDBP Human)
    

    Tags

    V5-MCP

    Expression

    Mammalian

    Mutation

    none

    Promoter

    EF-1a

    Available Since

    Nov. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. TFORF0275

    Plasmid

    #142582

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    TCF3 (TCF3 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Feb. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pHR- flag-Lag16-Ecad

    Plasmid

    #205197

    Purpose

    This vector encodes of the synCAM (Ecad) and GFP nanobody LAG16

    Depositor

    Wendell Lim

    Article

    Stevens et al Nature. 2023 Feb;614(7946):144-152. doi:

    Insert

    synCAM Ecad and Lag16 anti-GFP nanobody
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Sept. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pTriEx-RhoA FLARE.sc Biosensor WT

    Plasmid

    #12150

    Depositor

    Klaus Hahn

    Article

    Pertz et al Nature. 2006 Apr 20. 440(7087):1069-72.

    Insert

    RhoA Biosensor (RHOA Human)
    

    Tags

    6xHis

    Expression

    Bacterial, Insect, and Mamm…

    Available Since

    Aug. 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge