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80,098 results

Showing: 9421 - 9440 of 80098 results

  3. pαH-rS2d-HexaPro

    Plasmid
    #183515
    Purpose
    Mammalian cell expression of SARS-CoV-2 Spike protein rS2d-HexaPro with (682-685) furin side replaced with GSAS and mutation S383C, D985C, F817P, A892P, A899P, A942P, K986P,V987P
    Depositor
    Priyamvada Acharya
    Article
    Gobeil et al Mol Cell. 2022 Jun 2;82(11):2050-2068.e6
    Insert
    Spike (S-GSAS-rS2d.HexaPro) (S Severe acute respiratory syndrome coronavirus 2)
    Tags
    2X Strep-Tag II, 8X His tag, and HRV3C cleavage s…
    Expression
    Mammalian
    Mutation
    Ectodomain (AAs 1-1208); 682-685 (furin site = RR…
    Available Since
    May 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    183515-plasmid-map-sequence-id-362649

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  6. mEos3.2-TOMM20-N-10

    Plasmid
    #57483
    Purpose
    Localization: Mitochondrial Membrane, Excitation: 507 / 572, Emission: 516 / 580
    Depositor
    Michael Davidson
    Insert
    TOMM20 (TOMM20 Human)
    Tags
    mEos3.2
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Feb. 5, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  8. pLV-U6-SIPZ

    Plasmid
    #174848
    Purpose
    empty vector for cloning shRNA and/or miRNA
    Depositor
    Helen Morrison
    Type
    Empty backbone
    Use
    Lentiviral and RNAi
    Expression
    Mammalian
    Available Since
    Oct. 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    174848-plasmid-map-sequence-id-344690

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  9. pcDNA3.1-GGamma5-GFP2

    Plasmid
    #166777
    Purpose
    Encodes a G gamma subunit containing GFP2 as an "non-optimal" component of a BRET2 biosensor for studying heterotrimeric G protein dissociation
    Depositor
    Bryan Roth
    Article
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Insert
    GGamma5-GFP2 (GNG5 Human, Synthetic)
    Expression
    Mammalian
    Mutation
    Contains an N-terminal GFP2 and GSAG linker
    Promoter
    CMV
    Available Since
    May 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    166777-plasmid-map-sequence-id-408989

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  10. pcDNA3.1-GGamma3-GFP2

    Plasmid
    #166775
    Purpose
    Encodes a G gamma subunit containing GFP2 as an "non-optimal" component of a BRET2 biosensor for studying heterotrimeric G protein dissociation
    Depositor
    Bryan Roth
    Article
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Insert
    GGamma3-GFP2 (GNG3 Human, Synthetic)
    Expression
    Mammalian
    Mutation
    Contains an N-terminal GFP2 and GSAG linker
    Promoter
    CMV
    Available Since
    May 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    166775-plasmid-map-sequence-id-330996

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  18. pAAV-anti-PDL1(KN035) PAGER(TF)

    Plasmid
    #230003
    Purpose
    Expresses anti-PDL1 PAGER(TF) in mammalian cells; used with NanoLuc-Arrestin-TEVp (Addgene #125228) and UAS-Firefly Luciferase reporter (Addgene #104840)
    Depositor
    Alice Ting
    Article
    Kalogriopoulos et al Nature. 2024 Dec 4. doi: 10.1038/s41586-
    Insert
    IL2SP-Aro6-KN035-TEVcs-ALFA-KORD(RAA)-LOV-TEVcs-Gal4
    Use
    AAV
    Expression
    Mammalian
    Mutation
    V360A/R361A on KORD
    Promoter
    CMV
    Available Since
    Jan. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    230003-plasmid-map-sequence-id-457206

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  20. Gq-CASE

    Plasmid
    #168125
    Purpose
    Encodes a BRET-based activity sensor for the heterotrimeric G protein Gq. Composed of the subunits G alpha q (GNAQ) tagged with NanoLuciferase, G beta 3 (GNB3) and Venus-tagged G gamma 9 (GNG9).
    Depositor
    Gunnar Schulte
    Article
    Schihada et al Sci Signal. 2021 Sep 7;14(699):eabf1653.
    Insert
    GNAQ, GNB3, GNG9 (GNAQ, GNB3, GNGT2 Human)
    Use
    Luciferase
    Tags
    cpVenus on GNG9
    Expression
    Mammalian
    Mutation
    NLuc is inserted at F124/E125 within GNAQ
    Available Since
    July 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    168125-plasmid-map-sequence-id-332869

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Showing: 9421 - 9440 of 80098 results