78,128 results

1. plk1 WT GFP-myc

   Plasmid

   #132963

   Purpose

   To study the processes involved in early mitotic events, mitotic progression, and control of re-entry after DNA damage

   Depositor

   Michael Yaffe

   Article

   Kishi et al Mol Cell Biol. 2009 Jun;29(11):3134-50.

   Insert

   polo-like kinase 1 (PLK1 Human)
   

   Expression

   Mammalian

   Available Since

   Nov. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pIRESneo-EGFP-alpha Tubulin

   Plasmid

   #12298

   Depositor

   Patricia Wadsworth

   Article

   Rusan et al Mol Biol Cell. 2001 Apr . 12(4):971-80.

   Insert

   EGFP-alpha Tubulin (TUBA1B Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Available Since

   Aug. 11, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCIBN-EYFP-TetR

   Plasmid

   #103798

   Purpose

   BLInCR 'Localizer' construct that marks tetO arrays and is targeted by a PHR-tagged effector upon illumination with blue light

   Depositor

   Karsten Rippe

   Article

   Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

   Insert

   CIBN-EYFP-TetR (CIB1, tetR E. coli, Mustard Weed)
   

   Expression

   Mammalian

   Mutation

   TetR: A4G (M2V)

   Promoter

   CMV

   Available Since

   Jan. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLenti-Tet

   Plasmid

   #138086

   Purpose

   Empty vector with Tet-response promoter for inducible expression of proteins. Can be transduced using Lentiviral particles

   Depositor

   Eva Frickel

   Article

   Fisch et al EMBO J. 2019 Jul 1;38(13):e100926. doi:

   Type

   Empty backbone

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   minimal CMV promoter with heptamerized tet‐operat…

   Available Since

   March 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. EGFP-PIP5K1A

   Plasmid

   #202720

   Purpose

   expresses fluorescent protein-tagged enzyme

   Depositor

   Gerry Hammond

   Article

   Wills et al J Cell Sci. 2023 Aug 3:jcs.261494. doi:

   Insert

   EGFP:PIP5K1A (PIP5K1A Human)
   

   Expression

   Mammalian

   Available Since

   June 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3 Ras-LOCKR-S R89L (negative control)

   Plasmid

   #216497

   Purpose

   FRET-based reporter for Ras activity, untargeted, negative control

   Depositor

   David Baker

   Article

   Zhang et al Nat Biotechnol. 2024 Jan 25. doi: 10.103

   Insert

   Ras-LOCKR-S R89L negative control
   

   Expression

   Mammalian

   Available Since

   April 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. GFP-Ub KO

   Plasmid

   #11934

   Depositor

   Nico Dantuma

   Article

   Bergink et al Genes Dev. 2006 May 15. 20(10):1343-52.

   Insert

   Ubiquitin KO (RBX1 Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   All 7 lysines of ubiquitin mutated to arginines.

   Available Since

   Aug. 7, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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8. BE4

   Plasmid

   #100802

   Purpose

   Expresses BE4 in mammalian cells

   Depositor

   David Liu

   Article

   Komor et al Sci Adv. 2017 Aug 30;3(8):eaao4774. doi:

   Insert

   BE
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pORF1

   Plasmid

   #206025

   Purpose

   An expression vector for mouse LINE1 ORF1

   Depositor

   Masamichi Kamihira

   Article

   Zheng et al Biotechnol J. 2021 Jul;16(7):e2000620. d

   Insert

   ORF1 derived from mouse LINE1
   

   Expression

   Mammalian

   Available Since

   Dec. 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEGFP VAMP2

    Plasmid

    #42308

    Depositor

    Thierry Galli

    Article

    Martinez-Arca et al Proc Natl Acad Sci U S A. 2003 Jul 22;10

    Insert

    VAMP 2
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLVX-EF1alpha-SARS-CoV-2-nsp12-2xStrep-IRES-Puro

    Plasmid

    #141378

    Purpose

    Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus

    Depositor

    Nevan Krogan

    Article

    Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

    Insert

    SARS-CoV-2-nsp12 (ORF1ab SARS-CoV-2)
    

    Use

    Lentiviral

    Tags

    2xStrep

    Expression

    Mammalian

    Mutation

    human codon optimized

    Promoter

    EF1alpha

    Available Since

    April 9, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. 3xHA-TurboID pRetrox

    Plasmid

    #155203

    Purpose

    For doxycycline-inducible expression of 3xHA-TurboID on N-terminus of bait protein

    Depositor

    Kyle Roux

    Article

    May et al Cells. 2020 Apr 25;9(5). pii: cells90510

    Insert

    3xHA-TurboID
    

    Use

    Retroviral

    Tags

    3xHA

    Expression

    Mammalian

    Promoter

    tight TRE promotor

    Available Since

    Aug. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. Opto-CRAC version 1

    Plasmid

    #101245

    Purpose

    Genetically-encoded calcium actuator; mCherry-tagged LOV2-STIM1 chimera that enables optical control of calcium signals and calcium dependent signaling

    Depositor

    Yubin Zhou

    Article

    He et al Elife. 2015 Dec 8;4. pii: e10024. doi: 1

    Insert

    LOV2 and STIM1 cytosolic fragment
    

    Tags

    mCherry

    Expression

    Bacterial and Mammalian

    Promoter

    CMV

    Available Since

    April 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. LCV2 V-3

    Plasmid

    #84806

    Purpose

    Cas9 with sgRNA targeting Exon 1 of VEGF-A

    Depositor

    Glenn Yiu

    Article

    Yiu et al Invest Ophthalmol Vis Sci. 2016 Oct 1;57

    Insert

    gRNA targeting VEGF-A (VEGFA Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Nov. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. HaloTag-LaminA

    Plasmid

    #231894

    Purpose

    Expression of HaloTag-tagged LaminA to visualize the nuclear lamina

    Depositor

    Lukas Kapitein

    Article

    Korsten et al J Cell Biol. 2024 Nov 4;223(11):e2023071

    Insert

    LaminA (LMNA Human)
    

    Tags

    HaloTag

    Expression

    Mammalian

    Available Since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. WT NFAT2

    Plasmid

    #11101

    Depositor

    Anjana Rao

    Article

    Monticelli et al Eur J Immunol. 2002 Oct . 32(10):2971-8.

    Insert

    NFAT2 (Nfatc1 Mouse)
    

    Use

    Retroviral

    Tags

    3xHA and GFP

    Expression

    Mammalian

    Available Since

    April 19, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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17. GFP-PBRM1

    Plasmid

    #65387

    Purpose

    GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

    Depositor

    Kyle Miller

    Article

    Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

    Insert

    PBRM1 (PBRM1 Human)
    

    Tags

    EmGFP and V5

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available Since

    May 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLenti-hSynapsin-Cre-WPRE

    Plasmid

    #86641

    Purpose

    Expresses Cre in mammalian cells

    Depositor

    Fan Wang

    Article

    Sakurai et al Neuron. 2016 Nov 23;92(4):739-753. doi:

    Insert

    Cre
    

    Use

    Cre/Lox, Lentiviral, Mouse Targeting, and Synthet…

    Expression

    Mammalian

    Promoter

    hSynapsin

    Available Since

    Feb. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV2-GCaMP6f-P2A-H2B-PAmCherry

    Plasmid

    #133419

    Purpose

    Fluorescent reporter for calcium imaging and photoactivation

    Depositor

    Tim Holy

    Article

    Lee et al Science. 2019 Dec 13;366(6471):1384-1389

    Insert

    GCaMP6f-P2A-H2B-PAmCherry (H2BC11 Human)
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Dec. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSLQ7534 pHR (hU6-crSURF1-EFS-PuroR-WPRE)

    Plasmid

    #214878

    Purpose

    Lentiviral vector encoding RfxCas13d targeting SURF1 guide array

    Depositor

    Stanley Qi

    Article

    Tieu et al Cell. 2024 Feb 29;187(5):1278-1295.e20.

    Insert

    hU6-crSURF1-EFS-PuroR-WPRE
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    April 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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