78,128 results

1. pCAG-mEGFP-CaMKIIa (T286D/T305A/T306A)

   Plasmid

   #127392

   Purpose

   Fluorescent reporter for mutant Ca2+/calmodulin-dependent protein kinase II alpha (T286D/T305A/T306A)

   Depositor

   Ryohei Yasuda

   Article

   Chang et al Nat Commun. 2019 Jun 25;10(1):2784. doi:

   Insert

   calcium/calmodulin-dependent protein kinase II alpha (Camk2a Rat)
   

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   changed Threonine 286 to Aspartic acid and Threon…

   Promoter

   pCAG

   Available Since

   July 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. XLone-ETV2

   Plasmid

   #135763

   Purpose

   expresses human ETV2 upon doxycycline treatment

   Depositor

   Xiaojun Lian

   Article

   Ream et al Stem Cell Reports. 2024 Mar 5:S2213-6711

   Insert

   ETV2 (ETV2 Human)
   

   Expression

   Mammalian

   Mutation

   none

   Promoter

   TRE3G

   Available Since

   March 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMCL-HA-MAPKK1-WT

   Plasmid

   #40808

   Depositor

   Natalie Ahn

   Article

   Mansour et al Science. 1994 Aug 12;265(5174):966-70.

   Insert

   MAP2K1 (MAP2K1 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   wild type

   Promoter

   CMV

   Available Since

   Oct. 18, 2012

   Availability

   Academic Institutions and Nonprofits only

4. Alpha 5 integrin-GFP

   Plasmid

   #15238

   Depositor

   Rick Horwitz

   Article

   Laukaitis et al J Cell Biol. 2001 Jun 25. 153(7):1427-40

   Insert

   alpha 5 integrin (ITGA5 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Available Since

   Sept. 13, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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5. mCyRFP1-C1

   Plasmid

   #84355

   Purpose

   Expresses mCyRFP1 fluorescent protein in an empty backbone

   Depositor

   Ryohei Yasuda

   Article

   Laviv et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

   Insert

   mCyRFP1
   

   Expression

   Mammalian

   Mutation

   A161K and N41A, reletive to CyRFP1

   Promoter

   CMV

   Available Since

   Nov. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. Flag-cIAP2/pRK5

   Plasmid

   #27973

   Depositor

   Xiaolu Yang

   Article

   Hu et al J Clin Invest. 2006 Jan . 116(1):174-81.

   Insert

   CIAP2 (BIRC3 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 2, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSV human p85 alpha (HA tag)

   Plasmid

   #11499

   Depositor

   Ronald Kahn

   Article

   Ueki et al J Biol Chem. 2003 Nov 28. 278(48):48453-

   Insert

   p85 alpha (PIK3R1 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Available Since

   July 19, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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8. p-miniTurbo

   Plasmid

   #209636

   Purpose

   Expression miniTurbo in mammalian cells

   Depositor

   Xiang Zhou

   Article

   Lu et al Nucleic Acids Res. 2024 Mar 7:gkae126. d

   Insert

   miniTurbo
   

   Expression

   Mammalian

   Available Since

   April 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-EF1α-FLEX-rc[Chronos-GFP]

   Plasmid

   #62725

   Purpose

   AAV-mediated expression of Chronos-GFP under the EF1α promoter, in floxed/reversed (Cre-dependent) manner. Using bGHpA signal.

   Depositor

   Edward Boyden

   Article

   Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

   Insert

   Chronos-GFP
   

   Use

   AAV

   Tags

   GFP

   Expression

   Mammalian

   Promoter

   EF1α

   Available Since

   April 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pROSA26-ERT2CreERT2

    Plasmid

    #149436

    Purpose

    ROSA26 targetting vector with tamoxifen-inducible Cre recombinase

    Depositor

    Primo Schaer

    Article

    Schwarz et al F1000Res. 2020 Sep 9;9:1112. doi: 10.126

    Insert

    ERT2-Cre-ERT2 fusion protein
    

    Use

    Cre/Lox and Mouse Targeting

    Tags

    ERT2

    Expression

    Mammalian

    Promoter

    CAG promoter

    Available Since

    Sept. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRluc-LC3wt

    Plasmid

    #105002

    Purpose

    Mammalian expression of Rluc-LC3wt. Can be used for measuring autophagic flux.

    Depositor

    Marja Jããttelã

    Article

    Farkas et al Methods Enzymol. 2017;588:1-13. doi: 10.

    Insert

    LC3B (Map1lc3b Rat)
    

    Use

    Luciferase

    Tags

    Rluc with C124A substitution

    Expression

    Mammalian

    Available Since

    Jan. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. EF1a_Puro_Telo_v2

    Plasmid

    #195139

    Purpose

    ~100 repeats of the human telomere seed sequence fused to the puromycin resistance gene; digest with BstZ17I-HF and KpnI-HF to obtain transfectable construct for chromosomal arm loss induction

    Depositor

    Jason Sheltzer

    Article

    Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

    Insert

    Telomere (RTEL1 Human)
    

    Expression

    Mammalian

    Available Since

    March 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. CMV-H2B-mScarlet-I

    Plasmid

    #182297

    Purpose

    Localization of mScarlet-I fluorescent protein to histone 2B

    Depositor

    Severine Degrelle, Thierry Fournier

    Article

    Degrelle et al Environ Int. 2022 Sep 24;169:107545. doi

    Insert

    mScarlet-I
    

    Tags

    mScarlet-I

    Expression

    Mammalian

    Available Since

    Oct. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAG654 pFAST

    Plasmid

    #172861

    Purpose

    Expresses pFAST in mammalian cells

    Depositor

    Arnaud Gautier

    Article

    Benaissa et al Nat Commun. 2021 Nov 30;12(1):6989. doi:

    Insert

    pFAST
    

    Tags

    Myc tag (C terminal on insert)

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLVX-TetOne-Puro-EcSTH-FLAG

    Plasmid

    #218628

    Purpose

    This plasmid contains human codon optimized sequence of EcSTH which is a soluble transhydrogenase from E. coli that can be used to increase NADH/NAD+ ratio in mammalian cells.

    Depositor

    Valentin Cracan

    Article

    Pan et al Nat Chem Biol. 2023 Oct 26. doi: 10.1038

    Insert

    EcSTH
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    FLAG

    Expression

    Mammalian

    Available Since

    May 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCAGGS_cAMPFIRE-L

    Plasmid

    #182283

    Purpose

    Mammalian expression of the cAMP sensor cAMPFIRE-L under a CAG promotor w/ WPRE translation enhancer.

    Depositor

    Haining Zhong

    Article

    Massengill et al Nat Methods. 2022 Nov;19(11):1461-1471.

    Insert

    cAMPFIRE-L (cAMP sensor)
    

    Expression

    Mammalian

    Available Since

    March 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. FLAG-PRMT6

    Plasmid

    #164697

    Purpose

    Expression of PRMT6 with an N-terminal FLAG tag

    Depositor

    Cheryl Arrowsmith

    Article

    Eram et al ACS Chem Biol. 2016 Mar 18;11(3):772-781

    Insert

    PRMT6 (PRMT6 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. mCherry-Clathrin LC-15

    Plasmid

    #55019

    Purpose

    Localization: Clathrin Vesicles, Excitation: 587, Emission: 610

    Depositor

    Michael Davidson

    Article

    Rizzo et al Cold Spring Harb Protoc. 2009 Dec;2009(1

    Insert

    Clathrin LC (CLTB Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCMV-eNme2-T.1-ABE8e

    Plasmid

    #185668

    Purpose

    Expresses eNme2-T.1-ABE8e in mammalian cells

    Depositor

    David Liu

    Article

    Huang et al Nat Biotechnol. 2022 Sep 8. pii: 10.1038

    Insert

    ecTadA(8e)-neNme2-T.2
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    Nme2Cas9 D16A/E47K/V68M/T123A/D152G/E154K/T396A/H…

    Available Since

    Sept. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. p11L2w

    Plasmid

    #37331

    Depositor

    John Schiller

    Article

    Kieback et al Virology. 2006 Feb 5;345(1):199-208. Epu

    Insert

    HPV11 L2
    

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    July 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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