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pSEMS-CV SUy-Halo7Tag

Plasmid

#108933

Purpose

Selflabeling tag at ATP Synthase

Depositor

Karin Busch

Article

Wilkens et al J Cell Sci. 2013 Jan 1;126(Pt 1):103-16.

Insert

ATP synthase F1 subunit gamma (ATP5F1C Human)

Tags

Halo7 tag

Expression

Mammalian

Promoter

CMV

Available Since

June 25, 2018

Availability

Academic Institutions and Nonprofits only

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pCiGSΔΨ

Plasmid

#108312

Purpose

Foamy virus helper plasmid expressing Gag, driven by a CMV promoter. Ampicillin resistant.

Depositor

David Russell

Article

Josephson et al Hum Gene Ther. 2004 Jan;15(1):87-92.

Insert

gag

Expression

Mammalian

Mutation

pCiGSΔΨ is a newer version of the pCiGS Gag expre…

Promoter

CMV

Available Since

June 22, 2018

Availability

Academic Institutions and Nonprofits only

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pHR-EF1a-Archon1-KGC-EGFP-ER2

Plasmid

#108425

Purpose

Lentivirus production plasmid encoding for Archon1 fluorescent voltage reporter

Depositor

Edward Boyden

Article

Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

Insert

Archon1-KGC-EGFP-ER2

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

April 10, 2018

Availability

Academic Institutions and Nonprofits only

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cacnb2a (rat) in pMT2 vector

Plasmid

#107424

Purpose

Expresses Calcium channel beta2a auxillary subunit in mammalian cells

Depositor

Annette Dolphin

Article

Wyatt et al J Physiol. 1998 Jul 15;510 ( Pt 2):347-6

Insert

calcium channel beta2a auxillary subunit (Cacnb2 Rat)

Expression

Mammalian

Promoter

Ad MLP

Available Since

March 30, 2018

Availability

Academic Institutions and Nonprofits only

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pGL3 3'UTR reporter WT 1.3 kb CD274 Hs 3'UTR Final

Plasmid

#107009

Purpose

Modified Luciferase expression vector

Depositor

Julian Downward

Article

Coelho et al Immunity. 2017 Dec 19;47(6):1083-1099.e6

Insert

CD274 (CD274 Human)

Use

Luciferase

Expression

Mammalian

Available Since

March 23, 2018

Availability

Academic Institutions and Nonprofits only

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pmCherry-C1-mTrim21ΔPRYSPRY

Plasmid

#105518

Purpose

Expression of mCherry-tagged mouse Trim21 with a deleted PRYSPRY domain in mammalian cells

Depositor

Melina Schuh

Article

Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31

Insert

TRIM21 (Trim21 Mouse)

Tags

mCherry

Expression

Mammalian

Mutation

deletion of PRYSPRY domain (aa1-281)

Promoter

CMV

Available Since

March 7, 2018

Availability

Academic Institutions and Nonprofits only

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pRVdGL-4Cre

Plasmid

#98039

Purpose

Second-generation rabies viral vector genome

Depositor

Ian Wickersham

Article

Chatterjee et al Nat Neurosci. 2018 Apr;21(4):638-646. do

Insert

mCre

Expression

Mammalian

Promoter

CMV

Available Since

March 6, 2018

Availability

Academic Institutions and Nonprofits only

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pRFP-NLS

Plasmid

#105296

Purpose

To visualize nuclear dynamics in living cells

Depositor

Kenneth Yamada

Article

Petrie et al Science. 2014 Aug 29;345(6200):1062-5. d

Insert

Rac1 (RAC1 Human)

Tags

RFP

Expression

Mammalian

Mutation

aa encoding 172-192

Available Since

Feb. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFPN1-gamma-tubulin

Plasmid

#87956

Purpose

Expresses human gamma-tubulin with a GFP-tag on the C-terminal of the protein

Depositor

Maria Alvarado-Kristensson, Ana Carrera

Article

Alvarado-Kristensson et al Nat Cell Biol. 2009 Sep;11(9):1081-92. d

Insert

gamma-tubulin 1 (TUBG1 Human)

Tags

GFP

Expression

Mammalian

Mutation

The protein is a sh-gamma-tubulin resistant gene …

Available Since

Feb. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV.CAG.hChR2(H134R)-mCherry.WPRE.SV40

Plasmid

#100054

Purpose

AAV expression of humanized ChR2 with H134R mutation fused to mCherry driven by CAG promoter for optogenetic activation

Depositor

Karl Deisseroth

Has Service

AAV1, AAV2, AAV5, and AAV9

Insert

channelrhodopsin-2

Use

AAV

Tags

mCherry

Expression

Mammalian

Mutation

H134R mutation in ChR2

Promoter

CAG

Available Since

Jan. 31, 2018

Availability

Academic Institutions and Nonprofits only

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pRVL-2

Plasmid

#104580

Purpose

Contains mouse IgG2c CH1/CH2/CH3 domains, for cloning of antibody VH domains using SapI restriction enzyme. Full Fc-mediated immune effector functions

Depositor

Daniel Christ

Article

Vazquez-Lombardi et al Methods Mol Biol. 2018;1827:313-334. doi

Insert

Mouse IgG2c CH1/CH2/CH3

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 10, 2018

Availability

Academic Institutions and Nonprofits only

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CRY2high-mCherry

Plasmid

#104063

Purpose

Expresses fusion of CRY2high mutant (CRY2PHR E490R) with mCherry

Depositor

Bianxiao Cui

Article

Duan et al Nat Commun. 2017 Sep 15;8(1):547. doi: 1

Insert

CRY2high-mCherry

Expression

Mammalian

Mutation

CRY2PHR E490R

Promoter

CMV

Available Since

Dec. 14, 2017

Availability

Academic Institutions and Nonprofits only

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mCherry-iLIDx6

Plasmid

#103779

Purpose

expresses iPOLYMER component in mammalian cells

Depositor

Takanari Inoue

Article

Nakamura et al Nat Mater. 2017 Nov 6. pii: nmat5006. do

Insert

mCherry-iLIDx6

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 1, 2017

Availability

Academic Institutions and Nonprofits only

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pflareA-psd95-exon18.

Plasmid

#90249

Purpose

Minigene reporter for alternative splicing analysis

Depositor

Douglas Black

Article

Zheng et al Genome Res. 2013 Jun;23(6):998-1007. doi

Insert

psd95-exon18 (Dlg4 Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 26, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1+_Lambda-N-HA-Peptide

Plasmid

#92005

Purpose

Expresses Lambda-N-HA peptide

Depositor

Joshua Mendell

Article

Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

Insert

Lambda-N-HA peptide

Tags

Lambda-N-HA

Expression

Mammalian

Available Since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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BRAF_pLX307

Plasmid

#98322

Purpose

Lentiviral expression of BRAF

Depositor

William Hahn, Sefi Rosenbluh

Article

Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

Insert

BRAF (BRAF Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

E1Fa

Available Since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pScalps_Puro

Plasmid

#99636

Purpose

Empty backbone, 3rd generation lentiviral vector

Depositor

Silvia Monticelli

Article

Montagner et al Cell Rep. 2016 May 17;15(7):1566-79. doi

Type

Empty backbone

Use

Lentiviral

Tags

none

Expression

Mammalian

Promoter

SFFVp

Available Since

Aug. 22, 2017

Availability

Academic Institutions and Nonprofits only

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pmSTARRseq1

Plasmid

#96945

Purpose

CpG free plasmid for testing effects of methylation on regulatory activity

Depositor

Jenny Tung

Type

Empty backbone

Use

Mstarr-seq screening vector

Expression

Mammalian

Promoter

CpG -free EF1 promoter

Available Since

Aug. 21, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-dCas9-dMQ1-EGFP

Plasmid

#89637

Purpose

Targeted CpG methylation

Depositor

Margaret Goodell

Article

Lei et al Nat Commun. 2017 Jul 11;8:16026. doi: 10

Insert

site-specific DNA-methyltransferase SssI

Use

CRISPR

Tags

3*FLAG, 6*His, and T2A-EGFP

Expression

Mammalian

Mutation

C141S, TGC-->TCC; S317A, AGC-->GCC

Promoter

CMV

Available Since

July 12, 2017

Availability

Academic Institutions and Nonprofits only

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pEFIRES-P-mTagBFP-KDEL

Plasmid

#87163

Purpose

Fluorescent marker of endoplasmic reticulum (ER)

Depositor

Elina Ikonen

Article

Salo et al EMBO J. 2016 Dec 15;35(24):2699-2716. Ep

Insert

mTagBFP-KDEL

Tags

Bip signal peptide (Homo sapiens) and mTagBFP-KDE…

Expression

Mammalian

Promoter

EF-1 alpha

Available Since

May 30, 2017

Availability

Academic Institutions and Nonprofits only

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