78,131 results

1. pAAV-pCAG eGFP T2A SP HALO HAPLN1

   Plasmid

   #228200

   Purpose

   AAV vector expressing GFP and HaloTag-HAPLN1 under constitutive promoter

   Depositor

   Sungjin Park

   Article

   Sterin et al J Neurosci. 2024 Sep 9:e0666242024. doi:

   Insert

   HAPLN1 (Hapln1 Mouse)
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   constitutive CAG promoter

   Available Since

   Feb. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. mEmerald-Rab5a-7

   Plasmid

   #54243

   Purpose

   Localization: Ras GTPase, Excitation: 487, Emission: 509

   Depositor

   Michael Davidson

   Insert

   Rab5a
   

   Tags

   mEmerald

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 16, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pcDNA5-FRT/TO-3*Flag-APEX2 C-term PML

   Plasmid

   #187583

   Purpose

   Express FLAG epitope and APEX2-tagged PML fusion protein in mammalian cells

   Depositor

   Benjamin Blencowe

   Article

   Barutcu et al Mol Cell. 2022 Mar 3;82(5):1035-1052.e9.

   Insert

   PML (PML Human)
   

   Tags

   APEX2-FLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFLAG-SURF4

   Plasmid

   #170680

   Purpose

   Expresses FLAG-tagged SURF4 in mammalian cells.

   Depositor

   Ke Xu

   Article

   Yan et al Dev Cell. 2022 Feb 28;57(4):512-525.e8.

   Insert

   SURF4
   

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. AXBEv2

   Plasmid

   #204606

   Purpose

   Mammalian expression of evolved adenine transversion base editor AXBEv2

   Depositor

   Dali Li

   Article

   Chen et al Nat Biotechnol. 2023 Jun 15. doi: 10.103

   Insert

   bpNLS-TadA-8e-nSpCas9-mAAG (R165E/Y179F)-bpNLS-P2A-GFP
   

   Use

   CRISPR

   Tags

   6*His

   Expression

   Mammalian

   Mutation

   mAAG (R165E/Y179F); S. pyogenes Cas9 (D10A)

   Available Since

   Aug. 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLVX-EF1alpha-SARS-CoV-2-orf3a-2xStrep-IRES-Puro

   Plasmid

   #141383

   Purpose

   Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus

   Depositor

   Nevan Krogan

   Article

   Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

   Insert

   SARS-CoV-2-orf3a (ORF3a SARS-CoV-2)
   

   Use

   Lentiviral

   Tags

   2xStrep

   Expression

   Mammalian

   Mutation

   human codon optimized

   Promoter

   EF1alpha

   Available Since

   April 8, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pcDNA3HA-AR-LBD

   Plasmid

   #89111

   Purpose

   mammalian expression of human androgen receptor ligand binding domain amino acid residues 624-919 with N-terminal double HA tag

   Depositor

   Elizabeth Wilson

   Article

   He et al J Biol Chem. 1999 Dec 24;274(52):37219-2

   Insert

   hAR ligand binding domain (AR Human)
   

   Tags

   2xHA

   Expression

   Mammalian

   Mutation

   contains Ligand Binding Domain (624-919)

   Promoter

   CMV

   Available Since

   April 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMY-IRES-eGFP

   Plasmid

   #163361

   Purpose

   Retroviral backbone for in-frame cloning of a gene of interest and an IRES separated GFP marker

   Depositor

   Louis Ates, Teunis Geijtenbeek

   Article

   van der Donk et al Biol Open. 2022 Feb 15;11(2):bio059032.

   Type

   Empty backbone

   Use

   Retroviral

   Expression

   Mammalian

   Available Since

   Jan. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. TFORF1034

   Plasmid

   #143745

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   WT1 (WT1 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Sept. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. GFP-Trak1

    Plasmid

    #127621

    Purpose

    N-terminal GFP-tagged mouse Trak1

    Depositor

    Josef Kittler

    Article

    Birsa et al J Biol Chem. 2014 May 23;289(21):14569-8

    Insert

    Trak1 (Trak1 Mouse)
    

    Tags

    eGFP

    Expression

    Mammalian

    Available Since

    Feb. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. mouse Clock delta 19

    Plasmid

    #177312

    Purpose

    expression of mutant mouse clock

    Depositor

    Joseph Takahashi

    Article

    King et al Cell. 1997 May 16;89(4):641-53. doi: 10.

    Insert

    mouse clock delta 19
    

    Expression

    Mammalian

    Mutation

    deletion from original mClock gene (514-564aa)

    Promoter

    CMV

    Available Since

    Feb. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBA900

    Plasmid

    #122237

    Purpose

    Lentiviral CRISPR guide vector expressing a eGFP-NT2 sgRNA with cs2 incorporated at the 3' end of the sgRNA constant region.

    Depositor

    Jonathan Weissman

    Article

    Replogle et al Nat Biotechnol. 2020 Aug;38(8):954-961.

    Insert

    sgRNA with cs2 at the 3' end of the constant region
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    July 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. 3120 pSFFV-neo Bcl-XL

    Plasmid

    #8749

    Depositor

    Stanley Korsmeyer

    Article

    Chao et al J Exp Med 1995 Sep 1;182(3):821-8.

    Insert

    Bcl-xl (BCL2L1 Human)
    

    Expression

    Mammalian

    Available Since

    Feb. 13, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCAG-NCad-B1

    Plasmid

    #191667

    Purpose

    ddFP-B1-inserted mouse N-cadherin

    Depositor

    Takeharu Nagai

    Article

    Kanadome et al Commun Biol. 2022 Oct 7;5(1):1065. doi:

    Insert

    N-cadherin
    

    Tags

    HA and ddFP-B1

    Expression

    Mammalian

    Promoter

    Chicken β-actin promoter

    Available Since

    Oct. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSpCas9(BB)-2A-miRFP670

    Plasmid

    #91854

    Purpose

    Cas9 from S. pyogenes with 2A-miRFP670, and cloning backbone for sgRNA. Modified Zhang Plasmid #62988

    Depositor

    Ralf Kuehn

    Inserts

    hSpCas9-2A-miRFP670
    BB-guide RNA
    

    Use

    CRISPR

    Tags

    2A-miRFP670 and 3XFLAG

    Expression

    Mammalian

    Promoter

    Cbh and U6

    Available Since

    May 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. P2RY2-Tango

    Plasmid

    #66474

    Purpose

    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

    Depositor

    Bryan Roth

    Article

    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

    Insert

    P2RY2 (P2RY2 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

17. AAV-hSyn-Soma-jGCaMP8s

    Plasmid

    #169256

    Purpose

    AAV transfer plasmid for neuronal expression of soma-targeted jGCaMP8s.

    Depositor

    Marianne Fyhn

    Article

    Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

    Has Service

    AAV Retrograde, AAV1, and AAV9

    Insert

    Soma-jGCaMP8s
    

    Use

    AAV

    Tags

    6xHis-tag

    Expression

    Mammalian

    Available Since

    May 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMXs-Rex1

    Plasmid

    #13368

    Depositor

    Shinya Yamanaka

    Article

    Takahashi et al Cell. 2006 Aug 25. 126(4):663-76.

    Insert

    Zinc finger protein 42 (Zfp42 Mouse)
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    None

    Available Since

    Jan. 12, 2007

    Availability

    Academic Institutions and Nonprofits only

19. Flag-mRunx1c

    Plasmid

    #64894

    Purpose

    Lentiviral expression of Flag-tagged murine transcription factor Runx1c

    Depositor

    Georges Lacaud

    Article

    Batta et al Cell Rep. 2014 Dec 11;9(5):1871-84. doi:

    Insert

    Runx1c (Runx1 Mouse)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    June 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pIC247

    Plasmid

    #69741

    Purpose

    Retroviral expression of Borealin

    Depositor

    Iain Cheeseman

    Insert

    Borealin (CDCA8 Human)
    

    Use

    Retroviral

    Tags

    eYFP-TEV-S

    Expression

    Mammalian

    Available Since

    Nov. 4, 2015

    Availability

    Academic Institutions and Nonprofits only