80,109 results
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1530_pAAV-U6-SA-BbsI-MluI-gRNA-HLP-EmGFP-spA
Plasmid#109317PurposePlasmid for liver-specific expression of EmGFP with a BbsI cloning site for a gRNADepositorTypeEmpty backboneUseAAV and CRISPRExpressionMammalianAvailable SinceJan. 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
mEGFP-H4-23
Plasmid#56463PurposeLocalization: Nucleus/Histones, Excitation: 488, Emission: 507DepositorAvailable SinceDec. 23, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA3.1-CHRNA7-YFP
Plasmid#62630Purposemammalian expression of human Alpha7 (CHRNA7) with YFP fused into M3-M4 loopDepositorAvailable SinceMarch 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
qTAG-N-Zeo-dTAG
Plasmid#207705PurposeEmpty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Zeo-2A-dTAG cassetteDepositorTypeEmpty backboneUseCRISPR; Donor cassetteExpressionMammalianAvailable SinceNov. 15, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pEF_dCas9-VP64
Plasmid#68417PurposeTransient expression of Sp-dCas9 fused to the VP64 transcription activator, in mammalian cells, under an EF1-alpha promoter.DepositorInsertdCas9
UseCRISPRTags3xFLAG and VP64ExpressionMammalianMutationD10A/H841APromoterhuman EF1[alpha]Available SinceSept. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
PRKAR1A gRNA (BRDN0001146329)
Plasmid#77720Purpose3rd generation lentiviral gRNA plasmid targeting human PRKAR1ADepositorAvailable SinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
ALDH18A1
Plasmid#155402PurposeFor use in RBP tethering screenDepositorAvailable SinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
Luc-PS9
Plasmid#177942PurposeTransient mammalian expression of Luc2 (firefly luciferase) along with SARS-CoV-2 sequence 20080-21171 (PS9) within the 3'UTR. Resulting transcript is packaged into SARS-CoV-2 virus-like particles.DepositorInsertLuc2 (ORF1ab )
ExpressionMammalianAvailable SinceNov. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
human syndecan 2 wt
Plasmid#64970PurposeConstitutive expression in mammalian cellsDepositorAvailable SinceJune 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFRT/TO/GFP-SCAF8 (CRISPR_resistant)
Plasmid#122472PurposeDox-inducible GFP (N-terminal tag) SCAF8 expressionDepositorInsertSCAF8 (SCAF8 Human)
TagsGFPExpressionMammalianMutationSynonymous mutations conferring resistance to gRN…PromoterCMVAvailable SinceMay 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
PCDNA3-GFP1-9
Plasmid#121684PurposePPI reporterDepositorInsertGFP1-9
ExpressionMammalianAvailable SinceFeb. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
1477 pcDNA3 flag HA Akt1
Plasmid#9021DepositorAvailable SinceNov. 2, 2005AvailabilityAcademic Institutions and Nonprofits only -
DiLiCre 2.0
Plasmid#198752PurposeA Cre recombinase that is expressed upon doxycycline when combined with the UbC-blasticidine-P2A-TETon-3G transactivator. DiLiCre 2.0 is a cre recombinase that is activated by violet light.DepositorInsertCre-PhoCI-ERT2-ERT2
UseLentiviralTagsCre, kept in a photocleavable site, followed by 2…ExpressionBacterial and MammalianPromoterTRE3GVAvailable SinceJuly 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDONR221_SLC45A1
Plasmid#132289PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectorsDepositorInsertSLC45A1 (SLC45A1 Human)
ExpressionMammalianAvailable SinceNov. 12, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
Nano-lantern/pcDNA3
Plasmid#51970Purposemammalian expression of Nano-lantern, a luminescent protein for high-speed single-cell and whole body imagingDepositorInsertNano-lantern
ExpressionMammalianMutationS257G in Rluc8Available SinceMarch 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pUSE-Src-YF-UniRapR-mCerulean-myc
Plasmid#45381DepositorInsertSrc-YF-UniRapR-mCerulean-myc
TagsMyc, Myr, and mCeruleanExpressionMammalianMutationchanged mSrc Tyrosine 529 to PhenylalaninePromoterCMVAvailable SinceNov. 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
BII-ChBtW-iresFUCCI
Plasmid#133371PurposePiggybac vector for constitutive expressionDepositorInsertClover-Geminin-IRES-mKO2-Cdt1
TagsFlagExpressionMammalianMutationN/APromoterCMV enhancer and hEf1a (CpG free)Available SinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMVTNT-T7-KPNA7
Plasmid#26683DepositorAvailable SinceNov. 10, 2010AvailabilityAcademic Institutions and Nonprofits only -
hSERT-N1-pECFP
Plasmid#70106Purposemammalian expression of hSERT with C-terminal CFP fusionDepositorAvailable SinceOct. 21, 2015AvailabilityAcademic Institutions and Nonprofits only
