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pLenti-xBE4Gam-P2A-Puro

Plasmid

#112676

Purpose

Lentivirus for constitutive expression of xCas9(3.7)-BE4Gam in mammalian cells (codon optimized)

Depositor

Lukas Dow

Article

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

xCas9(3.7)-BE4Gam

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

D10A, A262T, R324L, S409I, E480K, E543D, M694I, E…

Promoter

EF1s

Available Since

July 30, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-IKZF1-V2-V5

Plasmid

#107390

Purpose

Mammalian expression of IKZF1-V2-V5

Depositor

William Kaelin

Article

Lu et al Science. 2014 Jan 17;343(6168):305-9. do

Insert

IKZF1-V2 (IKZF1 Human)

Tags

V5

Expression

Mammalian

Promoter

CMV

Available Since

July 25, 2018

Availability

Academic Institutions and Nonprofits only

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pMB-SaCas9

Plasmid

#105998

Purpose

SaCas9 expression vector

Depositor

Mike McManus

Article

Boettcher et al Nat Biotechnol. 2018 Jan 15. pii: nbt.40

Insert

Cas9 nuclease

Use

CRISPR and Lentiviral

Tags

none

Expression

Mammalian

Mutation

none

Promoter

short EF1alpha

Available Since

July 19, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP-H1.5

Plasmid

#32898

Depositor

Michael Hendzel

Article

Th'ng et al J Biol Chem. 2005 Jul 29;280(30):27809-1

Insert

Histone H1.5 (H1-5 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

May 19, 2018

Availability

Academic Institutions and Nonprofits only
FKBP-C-TEV 219

Plasmid

#100096

Purpose

SNIPer plasmid, C terminal TEV fused to FKBP

Depositor

Jim Wells

Article

Morgan et al Proc Natl Acad Sci U S A. 2015 Jul 7;112

Insert

C-TEV

Use

Retroviral

Tags

FKBP and Myc

Expression

Mammalian

Mutation

C terminal domain

Promoter

CMV

Available Since

May 14, 2018

Availability

Academic Institutions and Nonprofits only

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RINS1

Plasmid

#107290

Purpose

Plasmid for the expression of the RINS1 sensor

Depositor

Dmytro Yushchenko

Article

Schifferer et al Cell Chem Biol. 2017 Apr 20;24(4):525-53

Insert

RINS1

Tags

sfGFP

Expression

Mammalian

Promoter

CMV

Available Since

May 1, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP-Zdk1-EB1C

Plasmid

#107696

Purpose

C-terminal half of π-EB1, EGFP-tagged, dissociates from microtubule ends in blue light

Depositor

Torsten Wittmann

Article

van Haren et al Nat Cell Biol. 2018 Jan 29. pii: 10.1038

Insert

MAPRE1 (MAPRE1 Human)

Tags

EGFP and Zdk1

Expression

Mammalian

Mutation

EB1 aa 186-268

Promoter

CMV

Available Since

May 1, 2018

Availability

Academic Institutions and Nonprofits only

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MSCV-mouse Ezh2-IRES-GFP

Plasmid

#107146

Purpose

Mammalian expression of mouse Ezh2

Depositor

Martine Roussel

Insert

Ezh2 (Ezh2 Mouse)

Use

Retroviral

Expression

Mammalian

Available Since

April 3, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP Tensin1

Plasmid

#105297

Purpose

To study localization and function of Tensin1

Depositor

David Critchley, Kenneth Yamada

Article

Clark et al J Cell Biochem. 2010 Mar 1;109(4):808-17

Insert

Tensin1 (TNS1 Human)

Tags

EGFP

Expression

Mammalian

Available Since

Feb. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pADSL-EGFP

Plasmid

#99109

Purpose

Expresses human ADSL-EGFP fusion in mammalian cells

Depositor

Stephen Benkovic

Article

An et al Science. 2008 Apr 4;320(5872):103-6. doi

Insert

ADSL (ADSL Human)

Tags

EGFP (enhanced green fluorescent protein)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pCAGGS-ChrimsonR-mRuby2-ST

Plasmid

#105447

Purpose

Cation channelrhodopsin ChrimsonR fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendrites

Depositor

Hillel Adesnik

Article

Pegard et al Nat Commun. 2017 Oct 31;8(1):1228. doi:

Insert

ChrimsonR-mRuby2-ST

Tags

mRuby2 and soma targeting motif

Expression

Mammalian

Mutation

K176R in Chrimson

Promoter

pCAGGS

Available Since

Feb. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pFRT_TO_FlagHA_TIA1

Plasmid

#106093

Purpose

gene expression of FLAGHA-TIA1

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2018 Feb 15;69(4):622-635.e6.

Insert

TIA1 (TIA1 Human)

Tags

FlagHA

Expression

Mammalian

Available Since

Feb. 9, 2018

Availability

Academic Institutions and Nonprofits only

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MMW1578: CAG-human dLbCpf1(D832A)-NLS-3xHA

Plasmid

#104563

Purpose

Mammalian expression vector for catalytically inactive Cpf1 from Lachnospiraceae bacterium (dLbCpf1)

Depositor

Keith Joung

Article

Tak et al Nat Methods. 2017 Oct 30. doi: 10.1038/n

Insert

dLbCpf1 (D832A)

Use

CRISPR

Tags

NLS-3xHA

Expression

Mammalian

Mutation

D832A

Promoter

CAG

Available Since

Jan. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pC-HBH

Plasmid

#88896

Purpose

Carries phiC31 integrase attB site flanked by cHS4 insulators.

Depositor

Michele Calos

Article

Zhao et al PLoS One. 2014 Apr 29;9(4):e96279. doi:

Type

Empty backbone

Expression

Mammalian

Promoter

CAG

Available Since

Jan. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pGFP-ATIC

Plasmid

#99110

Purpose

Expresses human GFP-ATIC fusion in mammalian cells

Depositor

Stephen Benkovic

Article

An et al Science. 2008 Apr 4;320(5872):103-6. doi

Insert

ATIC (ATIC Human)

Tags

GFP

Expression

Mammalian

Mutation

S65T mutation in GFP gene

Promoter

CMV

Available Since

Dec. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV.hSyn.CaMPARI.WPRE.SV40

Plasmid

#100832

Purpose

AAV expression of CaMPARI, a photoconvertible fluorescent protein-based calcium integrator

Depositor

Loren Looger, Eric Schreiter

Article

Fosque et al Science. 2015 Feb 13;347(6223):755-60. d

Has Service

AAV1, AAV5, and AAV9

Insert

CaMPARI

Use

AAV

Expression

Mammalian

Promoter

Synapsin

Available Since

Nov. 9, 2017

Availability

Academic Institutions and Nonprofits only

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pHR_Gal4UAS_humanTRAIL _IRES_mCherry_PGK_tBFP

Plasmid

#85432

Purpose

Lentiviral vector for Gal4 inducible human full-length TRAIL with mCherry reporter and constitutive tBFP expression

Depositor

Wendell Lim

Article

Roybal et al Cell. 2016 Oct 6;167(2):419-432.e16. doi

Insert

human TRAIL

Use

Lentiviral

Expression

Mammalian

Available Since

Sept. 19, 2017

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-TYMS

Plasmid

#100545

Purpose

mammalian expression of thymidylate synthetase

Depositor

Yuh-Shan Jou

Article

Yeh et al Oncotarget. 2017 Sep 8;8(44):77734-77751

Insert

thymidylate synthetase (TYMS Human)

Expression

Mammalian

Available Since

Sept. 12, 2017

Availability

Academic Institutions and Nonprofits only

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CDA1-BE3

Plasmid

#100804

Purpose

Expresses CDA1-BE3 in mammalian cells

Depositor

David Liu

Article

Komor et al Sci Adv. 2017 Aug 30;3(8):eaao4774. doi:

Insert

CDA1-BE3

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 31, 2017

Availability

Academic Institutions and Nonprofits only

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BE3-Gam

Plasmid

#100807

Purpose

Expresses BE3-Gam in mammalian cells

Depositor

David Liu

Article

Komor et al Sci Adv. 2017 Aug 30;3(8):eaao4774. doi:

Insert

BE3-Gam

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 31, 2017

Availability

Academic Institutions and Nonprofits only

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