78,133 results

1. pmCherryC1 VAP-A

   Plasmid

   #226407

   Purpose

   Expression of human VAP-A fused to mCherry in mammalian cells

   Depositor

   Catherine Tomasetto

   Article

   Di Mattia et al EMBO Rep. 2018 Jul;19(7). pii: embr.2017

   Insert

   VAP-A (VAPA Human)
   

   Tags

   T7-Xpress tag and mCherry

   Expression

   Mammalian

   Available Since

   Oct. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pcDNA3-TDsmURFP-IRES-HO-1

   Plasmid

   #80346

   Purpose

   Expresses Tandem Dimer smURFP and Heme Oxygenase-1 in mammalian cells.

   Depositor

   Erik Rodriguez, Roger Tsien

   Article

   Rodriguez et al Nat Methods. 2016 Aug 1. doi: 10.1038/nm

   Inserts

   Tandem Dimer smURFP
   HO-1
   

   Expression

   Mammalian

   Promoter

   CMV and IRES

   Available Since

   Aug. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pLentiCMVNeoDEST(705-1)-hAXL

   Plasmid

   #124796

   Purpose

   Expresses AXL in mammalian cells

   Depositor

   Kenneth Pienta

   Article

   Axelrod et al Mol Cancer Res. 2019 Feb;17(2):356-369.

   Insert

   AXL (AXL Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCMV5 TBRI-HA with various GS domain mutations

   Plasmid

   #19162

   Depositor

   Joan Massague

   Insert

   transforming growth factor, beta receptor 1 (TGFBR1 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   T204D (GS domain mutation)

   Available Since

   Sept. 3, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pcDNA4-GFP-Vinculin

   Plasmid

   #107153

   Purpose

   mammalian expression

   Depositor

   Tina Izard

   Article

   Chinthalapudi et al J Cell Biol. 2014 Dec 8;207(5):643-56. d

   Insert

   Vinculin (VCL Human)
   

   Tags

   (His)10-GFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pBGSA C5aR1-EGFP

   Plasmid

   #62612

   Purpose

   Constitutive expression of C5aR1-EGFP in mammalian cells

   Depositor

   Heini Miettinen

   Article

   Suvorova et al Traffic. 2005 Feb;6(2):100-15.

   Insert

   C5aR1 (C5AR1 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   SRalpha

   Available Since

   June 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. mTagRFP-Membrane-1

   Plasmid

   #57992

   Purpose

   Localization: Plasma Membrane, Excitation: 555, Emission: 584

   Depositor

   Michael Davidson

   Type

   Empty backbone

   Tags

   N-terminal 20 Aas MLS from Neuromodulin and mTagR…

   Expression

   Mammalian

   Available Since

   Dec. 5, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

8. Mito-mGreenLantern

   Plasmid

   #164461

   Purpose

   Localization of mGreenLantern fluorescent protein to mitochondria

   Depositor

   Gregory Petsko

   Article

   Campbell et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

   Insert

   mGreenLantern
   

   Tags

   Human COX8a first 29 amino acids

   Expression

   Mammalian

   Mutation

   Clover-F64L/S72A/E124V/N149K/I167T/S175G/A206K/L2…

   Promoter

   CMV

   Available Since

   Jan. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. TFORF1906

   Plasmid

   #141883

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   TCF7L2 (TCF7L2 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pEGFP-N1-Cdc45

    Plasmid

    #211426

    Purpose

    mammalian expression of human Cdc45 C-terminally fused to EGFP

    Depositor

    Helmut Pospiech

    Article

    Kohler et al Cell Cycle. 2016;15(7):974-85. doi: 10.1

    Insert

    Cdc45 (CDC45 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pINDUCER-21-RUNX1

    Plasmid

    #97043

    Purpose

    Expresses RUNX1 in mammalian cells

    Depositor

    George Daley

    Article

    Sugimura et al Nature. 2017 May 17. doi: 10.1038/nature

    Insert

    Runt Related Transcription Factor 1 (RUNX1 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    Tet on

    Available Since

    Feb. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

12. LeGO-S2

    Plasmid

    #85211

    Purpose

    Lentiviral vector expressing T-Sapphire fluorescent protein driven by SFFV promoter

    Depositor

    Boris Fehse

    Article

    Mohme et al Mol Ther. 2017 Jan 18. pii: S1525-0016(1

    Insert

    T-Sapphire
    

    Use

    Cre/Lox and Lentiviral

    Expression

    Mammalian

    Promoter

    SFFV

    Available Since

    Jan. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAAV-GFAP-hChR2(H134R)-mCherry

    Plasmid

    #27055

    Purpose

    AAV expression of humanized ChR2(H134R) fused to mCherry, for optogenetic excitation

    Depositor

    Karl Deisseroth

    Insert

    hChR2(H134R)
    

    Use

    AAV

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    H134R

    Promoter

    GFAP

    Available Since

    Jan. 27, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. qTAG-C-ultraID-Blast

    Plasmid

    #207715

    Purpose

    Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a ultraID-2A-Blast cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Expression

    Mammalian

    Available Since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

15. mCherry-Calnexin-N-14

    Plasmid

    #55005

    Purpose

    Localization: Endoplasmic Reticulum, Excitation: 587, Emission: 610

    Depositor

    Michael Davidson

    Insert

    Calnexin (CANX Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    I474N in Calnexin

    Promoter

    CMV

    Available Since

    Aug. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Stx5S-mCherry

    Plasmid

    #165087

    Purpose

    Expresses Stx5S-mCherry in mammalian cells

    Depositor

    Geert van den Bogaart

    Article

    Linders et al Nat Commun. 2021 Oct 28;12(1):6227. doi:

    Insert

    Stx5 (Short isoform) (STX5 Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Lacks amino acids 1-54, enocdes short isoform of …

    Promoter

    CMV

    Available Since

    Nov. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pC-KIT8

    Plasmid

    #118990

    Purpose

    Mutational dissection of the G rich region of the c-kit promoter

    Depositor

    Bjorn Hogberg

    Article

    Ducani et al J Am Chem Soc. 2019 Jul 3;141(26):10205-

    Insert

    c-kit proto-oncogene {promoter} (KIT Human)
    

    Expression

    Mammalian

    Mutation

    mutK2-mutSP-mutK1

    Available Since

    June 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pDONR221_SLC16A9

    Plasmid

    #131876

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC16A9 (SLC16A9 Human)
    

    Expression

    Mammalian

    Available Since

    Oct. 9, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. AAV-U6-dgRNA-CAG-MPH

    Plasmid

    #106259

    Purpose

    Expresses MPH co-activator from the CAG promoter and one U6-driven dgRNA in AAV backbone

    Depositor

    Juan Belmonte

    Article

    Liao et al Cell. 2017 Dec 14;171(7):1495-1507.e15.

    Insert

    MS2-P65-HSF1 (HSF1 Synthetic, Human)
    

    Use

    AAV and CRISPR

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    May 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGP-CMV-jGCaMP7b

    Plasmid

    #104484

    Purpose

    Mammalian expression of ultrasensitive protein calcium sensor

    Depositor

    Douglas Kim, GENIE Project

    Article

    Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi

    Insert

    jGCaMP7b
    

    Tags

    T7 epitope, Xpress tag, 6xHis

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge