81,862 results

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pcFLAG-CSK-WT

Plasmid

#74502

Purpose

Overexpression of CSK in mammalian cells

Depositor

Lars Rönnstrand

Article

Kazi et al Cell Signal. 2013 Sep;25(9):1852-60. doi

Insert

CSK (CSK Human)

Tags

FLAG

Expression

Mammalian

Mutation

K222R

Promoter

CMV

Available Since

May 20, 2016

Availability

Academic Institutions and Nonprofits only
pLentiCRISPR-RFP657

Plasmid

#75162

Purpose

Derived from LentiCRISPR v1 but expresses RFP657 instead of puromycin resistance

Depositor

Beat Bornhauser

Article

McComb et al Sci Transl Med. 2016 May 18;8(339):339ra

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

RFP657

Expression

Mammalian

Available Since

May 17, 2016

Availability

Academic Institutions and Nonprofits only

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6xO/S luc

Plasmid

#69445

Purpose

detection/assessment of OCT4 and SOX2 transcription factor activity

Depositor

Lisa Dailey

Insert

minimal fgf4 promoter and 6XOS tandem repeat of composite OCT4/Sox2 binding site from FGF4 enhancer

Use

Luciferase

Expression

Mammalian

Available Since

May 5, 2016

Availability

Academic Institutions and Nonprofits only

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Lenti.pFOXA2.GFP

Plasmid

#59404

Purpose

expresses eGFP from a 400 bp human FOXA2 promoter to select floor plate and ventral midbrain progenitor cells during development

Depositor

Kai-Christian Sonntag

Article

Aguila et al Stem Cells Transl Med. 2014 Jul 14. pii:

Insert

400 bp FOXA2 promoter, eGFP (FOXA2 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

pFOXA2

Available Since

April 19, 2016

Availability

Academic Institutions and Nonprofits only

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Rlucrarr3-Flash5

Plasmid

#74133

Purpose

FlAsH-BRET reporter to monitor conformational changes in Arrestin3. Insertion of FlAsH motif (CCPGCC) following amino acid residue 263 of Arrestin.

Depositor

Louis Luttrell

Article

Lee et al Nature. 2016 Mar 31;531(7596):665-8. doi

Insert

Arrestin3

Tags

Renilla luciferase (rLuc)

Expression

Mammalian

Mutation

Insertion of FlAsH motif (CCPGCC) following amino…

Available Since

April 1, 2016

Availability

Academic Institutions and Nonprofits only
pMK278 (mClover-Hygro)

Plasmid

#72794

Purpose

C-terminal tagging with mClover using CRIPSR/Cas9

Depositor

Masato Kanemaki

Article

Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

Insert

mClover-Hygro

Use

CRISPR

Tags

mClover

Expression

Mammalian

Available Since

March 24, 2016

Availability

Academic Institutions and Nonprofits only

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pMK283 (Stag-3FLAG-Neo)

Plasmid

#72799

Purpose

C-terminal tagging with Stag-3FLAG using CRIPSR/Cas9

Depositor

Masato Kanemaki

Article

Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

Insert

Stag-3FLAG-Neo

Use

CRISPR

Tags

Stag-3FLAG

Expression

Mammalian

Available Since

March 24, 2016

Availability

Academic Institutions and Nonprofits only

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DIO-EF1a-FLEX-H2B-GFP-P2A-N2c(G)

Plasmid

#73476

Purpose

Encodes the G protein to enable transsynaptic RAV infection

Depositor

Thomas Jessell

Article

Reardon et al Neuron. 2016 Jan 19. pii: S0896-6273(16)

Insert

H2B-GFP-P2A-N2c(G)

Use

AAV

Expression

Mammalian

Available Since

March 21, 2016

Availability

Academic Institutions and Nonprofits only

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pLVX-FLAG-dTomato-centrin-1

Plasmid

#73332

Purpose

Lentiviral vector encoding centrin-1 with an N-terminal FLAG and dTomato tag

Depositor

Manuel Thery

Insert

centrin-1 (CETN1 Human)

Use

Lentiviral

Tags

FLAG and dTomato

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 29, 2016

Availability

Academic Institutions and Nonprofits only

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cpVenus-Gγ2

Plasmid

#69626

Purpose

The heterotrimeric G-protein subunit, Gγ2, tagged with circular permutated Venus (cp173V).

Depositor

Dorus Gadella

Article

van Unen et al PLoS One. 2016 Jan 22;11(1):e0146789. do

Insert

cp173Venus-Ggamma2 (GNG2 Synthetic)

Tags

ciruclar permutated Venus (cp173V)

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 25, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG-MTOR-V2006I

Plasmid

#69012

Purpose

activating MTOR mutation

Depositor

David Sabatini

Article

Grabiner et al Cancer Discov. 2014 May;4(5):554-63. doi

Insert

MTOR (MTOR Human)

Tags

FLAG

Expression

Mammalian

Mutation

V2006I

Promoter

CMV

Available Since

Jan. 20, 2016

Availability

Academic Institutions and Nonprofits only

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hSERT-N1-pEYFP

Plasmid

#70105

Purpose

mammalian expression of hSERT with C-terminal YFP fusion

Depositor

Harald Sitte

Article

Just et al J Biol Chem. 2004 Feb 20;279(8):6650-7.

Insert

SERT (SLC6A4 Human)

Tags

EYFP

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 16, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_PsChR2_EYFP

Plasmid

#69057

Purpose

High-efficiency blue-shifted cation channelrhodopsin

Depositor

John Spudich

Article

Govorunova et al J Biol Chem. 2013 Oct 11;288(41):29911-2

Insert

PsChR2

Tags

EYFP

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 13, 2015

Availability

Academic Institutions and Nonprofits only

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pLX302_HOXB13

Plasmid

#70089

Purpose

Expression of human HOXB13 from CMV promoter, puromycin selection marker

Depositor

William Hahn

Article

Pomerantz et al Nat Genet. 2015 Nov;47(11):1346-51. doi:

Insert

HOXB13 (HOXB13 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only

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FFA3-Tango

Plasmid

#66282

Purpose

Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

Depositor

Bryan Roth

Article

Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

Insert

FFA3 (FFAR3 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 15, 2015

Availability

Academic Institutions and Nonprofits only
GIPR-Tango

Plasmid

#66294

Purpose

Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

Depositor

Bryan Roth

Article

Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

Insert

GIPR (GIPR Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 15, 2015

Availability

Academic Institutions and Nonprofits only
Orange Nano-lantern/pcDNA3

Plasmid

#67661

Purpose

orange luminescent protein for high-speed single-cell and whole body imaging

Depositor

Takeharu Nagai

Article

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

Orange Nano-lantern

Expression

Mammalian

Available Since

Sept. 10, 2015

Availability

Academic Institutions and Nonprofits only
Str-STIM1-NN_ST-SBP-EGFP

Plasmid

#65262

Purpose

synchronize trafficking of ST from the ER (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

Str-STIM1-NN and truncated sialyltransferase (ST6GAL1 Human)

Tags

EGFP and Streptavidin Binding Protein (SBP)

Expression

Mammalian

Mutation

contains only amino acids 1 to 43 of ST

Promoter

CMV

Available Since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3 Flag Erg

Plasmid

#66977

Purpose

Expression plasmid for mouse Erg; adds Flag tag to the N-terminus of protein

Depositor

Christopher Vakoc

Article

Roe et al Mol Cell. 2015 May 12. pii: S1097-2765(1

Insert

Erg (Erg Mouse)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

July 21, 2015

Availability

Academic Institutions and Nonprofits only
Myc-E-Syt2

Plasmid

#66834

Purpose

Mammalian Expression, Bacterial Expression

Depositor

Pietro De Camilli

Article

Giordano et al Cell. 2013 Jun 20;153(7):1494-509. doi:

Insert

E-Syt2 (ESYT2 Human)

Tags

Myc

Expression

Mammalian

Available Since

July 21, 2015

Availability

Academic Institutions and Nonprofits only