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pAAV-EF1a-DIO-JEDI3sub-Kv-WPRE

Plasmid

#246616

Purpose

Double floxed soma and AIS-localized genetically encoded voltage indicator (GEVI) JEDI3sub in AAV production vector expressed under the mammalian promoter (EF1a)

Depositor

Francois St-Pierre

Article

Land et al Nat Methods. 2026 Apr 15. doi: 10.1038/s

Insert

JEDI3sub-Kv

Use

AAV and Cre/Lox

Tags

Kv

Expression

Mammalian

Promoter

EF1a

Available Since

April 23, 2026

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-JEDI3sub-Kv-WPRE

Plasmid

#246618

Purpose

Soma and AIS-localized genetically encoded voltage indicator (GEVI) JEDI3sub in AAV production vector for neuron -specific expression using the promoter hSyn

Depositor

Francois St-Pierre

Article

Land et al Nat Methods. 2026 Apr 15. doi: 10.1038/s

Insert

JEDI3sub-Kv

Use

AAV

Tags

Kv

Expression

Mammalian

Promoter

hSyn

Available Since

April 23, 2026

Availability

Academic Institutions and Nonprofits only

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pLX403_SMARCB1_WT

Plasmid

#233902

Purpose

Inducible vector with SMARCB1 variant 1

Depositor

Andrew Hong

Article

Cooper et al Nat Commun. 2026 Apr 8. doi: 10.1038/s41

Insert

SMARCB1 (SMARCB1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

TRE

Available Since

April 7, 2026

Availability

Academic Institutions and Nonprofits only

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pHAGE EGFP-mouse Parkin

Plasmid

#252269

Purpose

Lentiviral expression of EGFP-mouse Parkin

Depositor

David Chan

Article

Nakajima et al Curr Biol. 2026 Mar 9;36(5):1205-1219.e8

Insert

mouse Parkin (Prkn Mouse)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 25, 2026

Availability

Academic Institutions and Nonprofits only

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pEf1a-hifiDn29-P2A-GFP

Plasmid

#247154

Purpose

Mammalian expression of a human codon optimized engineered hifiDn29 recombinase (highest specificity variant)

Depositor

Patrick Hsu

Article

Fanton et al Nat Biotechnol. 2025 Nov 6. doi: 10.1038

Insert

hifiDn29-P2A-GFP

Tags

6xHis

Expression

Mammalian

Mutation

M6I/E70G/A224P/G227V/Q332K/N341K/L393P/D503N/I98V…

Promoter

Ef1a

Available Since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-TET1-dCas9

Plasmid

#235599

Purpose

Expresses Tet1CD-dCas9

Depositor

Ting Zhou

Article

Wu et al Stem Cell Reports. 2025 Apr 25:102499. d

Insert

TET1-dCas9 (TET1 Human, SpCas9 is from Streptococcus pyogenes)

Use

CRISPR and Lentiviral

Tags

3xNLS and TET1 CD

Expression

Mammalian

Promoter

EF1a

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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GFP-mCAMSAP3-Myc

Plasmid

#236536

Purpose

For expression of CAMSAP3 (mouse) in mammalian cells

Depositor

Sandra Citi

Article

Flinois et al MicroPubl Biol. 2024 Oct 14;2024:10.1791

Insert

CAMSAP3 (Camsap3 Mouse)

Tags

GFP and Myc

Expression

Mammalian

Mutation

WT

Available Since

April 16, 2025

Availability

Academic Institutions and Nonprofits only

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CAG-mCherry

Plasmid

#229135

Purpose

Retrovirus driving mCherry reporter

Depositor

Elizabeth Kirby

Article

Miller et al Mol Psychiatry. 2024 Nov 11. doi: 10.103

Insert

mCherry

Use

Retroviral

Expression

Mammalian

Available Since

Jan. 22, 2025

Availability

Academic Institutions and Nonprofits only

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pKM-U6-reRNA-hsEMX1

Plasmid

#205637

Purpose

reRNA guide targeting Homo sapiens EMX1

Depositor

Gerald Schwank

Article

Marquart et al Nat Methods. 2024 Sep 23. doi: 10.1038/s

Insert

reRNA-hsEMX1 (EMX1 Human)

Expression

Mammalian

Promoter

U6

Available Since

Sept. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pBactin-mOrange2-CI

Plasmid

#196852

Purpose

Expression of red fluorescent reporter mOrange driven by the strong Beta-actin promoter

Depositor

Frank Bradke

Article

Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

Insert

mOrange2

Expression

Mammalian

Mutation

None (wt)

Promoter

Chicken bactin (plus Chicken bactin intron)

Available Since

May 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV(MinDis)-iFluoxSnFR_cyto

Plasmid

#182817

Purpose

Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists

Depositor

Henry Lester

Article

Nichols et al J Neurosci. 2023 Mar 2:JN-RM-1519-22. do

Insert

iFluoxSnFR_cyto

Expression

Mammalian

Promoter

hCMV

Available Since

Jan. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pDONR221_SLC25A35

Plasmid

#132017

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC25A35 (SLC25A35 Human)

Expression

Mammalian

Available Since

Oct. 25, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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LentiV_Neo_LKB1_CR

Plasmid

#108112

Purpose

Lentiviral expression plasmid of human LKB1 cDNA (CRISPR-resistant silent mutation) with neomycin resistance gene

Depositor

Christopher Vakoc

Article

Tarumoto et al Mol Cell. 2018 Feb 28. pii: S1097-2765(1

Insert

LKB1 (STK11 Human)

Use

CRISPR and Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

change cytosine 333 to alanine (silent mutation)

Promoter

EFS promoter

Available Since

April 5, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-hPC1_MTS-eGFP

Plasmid

#108402

Purpose

Express putative mitochondrial targeting sequence of human PC1 from amino acid 4129 to 4157

Depositor

Gregory Germino

Article

Lin et al Sci Rep. 2018 Feb 9;8(1):2743. doi: 10.1

Insert

PKD1 (PKD1 Mouse)

Tags

3xHA and eGFP

Expression

Mammalian

Promoter

CMV

Available Since

April 2, 2018

Availability

Academic Institutions and Nonprofits only

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pLV-Bsd-CMV>tTS/rtTA:T2A:{cymR}

Plasmid

#236251

Purpose

Blasticidin selected lentiviral vector with Tet-On regulatory proteins and the cumate repressor, for use with dual-inducible CymR-CuO system

Depositor

Genevieve Kendall

Article

Kent et al Sci Rep. 2024 Sep 5;14(1):20773. doi: 10

Inserts

tTS/rtTA
cymR

Use

Lentiviral

Expression

Mammalian

Mutation

rTS and rtTA_M2 linked by T2A

Promoter

CMV

Available Since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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pUAS-NanoLuc

Plasmid

#87696

Purpose

Gal4VP16 driven expression of NanoLuc

Depositor

Robert Campbell

Article

Zhang et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

Insert

NanoLuc

Tags

nuclear localization signal

Expression

Mammalian

Available Since

April 26, 2017

Availability

Academic Institutions and Nonprofits only

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pLVX-Puro-TDP-43-WT

Plasmid

#133753

Purpose

Wild type human TDP-43 in pLVX-Puro lentiviral vector

Depositor

Shawn Ferguson

Article

Roczniak-Ferguson et al Life Sci Alliance. 2019 Sep 16;2(5). pii

Insert

TDP-43 (TARDBP Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pMOS007: PercevalHR ATP/ADP sensor (cytosolic)

Plasmid

#163061

Purpose

PercevalHR ATP/ADP sensor (cytosolic)

Depositor

Adam Cohen

Article

Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

Insert

PercevalHR

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-evoCAST-eCas7

Plasmid

#234726

Purpose

Engineered human-codon optimized PseCas7 in mammalian cells (for evoCAST)

Depositor

David Liu

Article

Witte et al Science. 2025 May 15;388(6748):eadt5199.

Insert

ePseCas7

Expression

Mammalian

Available Since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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pGL3-U6-sgRNA-PGK-puromycin

Plasmid

#51133

Purpose

expresses sgRNA from the U6 promoter.

Depositor

Xingxu Huang

Article

Shen et al Nat Methods. 2014 Mar 2. doi: 10.1038/nm

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Feb. 11, 2014

Availability

Academic Institutions and Nonprofits only

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