76,968 results

1. T7-VEE-IRES-Puro

   Plasmid

   #58970

   Purpose

   noninfectious (non-packaging), self-replicating Venezuelan equine encephalitis (VEE) virus RNA replicon

   Depositor

   Steven Dowdy

   Article

   Yoshioka et al Cell Stem Cell. 2013 Aug 1;13(2):246-54.

   Type

   Empty backbone

   Use

   Venezuelan equine encephalitis (vee) virus rna re…

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   26S subgenomic promoter

   Available Since

   Oct. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. mTFP1-N1

   Plasmid

   #54521

   Purpose

   Localization: N1 Cloning Vector, Excitation: 462, Emission: 492

   Depositor

   Robert Campbell, Michael Davidson

   Article

   Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

   Type

   Empty backbone

   Use

   Tags

   mTFP1

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 20, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pUCmini-iCAP-PHP.B

   Plasmid

   #103002

   Purpose

   non-standard AAV2 rep-AAV-PHP.B cap plasmid with AAV cap expression controlled by a tTA-TRE amplification system

   Depositor

   Viviana Gradinaru

   Article

   Deverman et al Nat Biotechnol. 2016 Feb;34(2):204-9. do

   Insert

   Synthetic construct isolate AAV-PHP.B VP1 gene
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   p41

   Available Since

   Feb. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pUAS-NanoLuc

   Plasmid

   #87696

   Purpose

   Gal4VP16 driven expression of NanoLuc

   Depositor

   Robert Campbell

   Article

   Zhang et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

   Insert

   NanoLuc
   

   Use

   Tags

   nuclear localization signal

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTRE-8XGTIIC-DsRED-DD

   Plasmid

   #115798

   Purpose

   lentiviral, trimethoprim (TMP) regulated YAP promoter activity reporter

   Depositor

   Joan Massague

   Article

   Er et al Nat Cell Biol. 2018 Aug;20(8):966-978. d

   Insert

   YAP responsive promoter (YAP1 Human)
   

   Use

   Lentiviral

   Tags

   DsRED-DHFR

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLentiCas9-BFP

   Plasmid

   #78545

   Purpose

   Fusion protein Cas9+BFP

   Depositor

   Rory Johnson, Roderic Guigo

   Article

   Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

   Insert

   BFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Fusion protein with Cas9

   Available Since

   July 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3-DN-hCUL1-FLAG

   Plasmid

   #15818

   Depositor

   Wade Harper

   Article

   Jin et al Methods Enzymol. 2005;399:287-309. doi:

   Insert

   Cullin1 (CUL1 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Dominant negative: N452

   Promoter

   Available Since

   Sept. 28, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEGFP-VSP4-E228Q

   Plasmid

   #80351

   Purpose

   Expression of GFP-tagged, dominant negative VPS4 mutant

   Depositor

   Wesley Sundquist

   Article

   Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

   Insert

   VPS4-E228Q (VPS4A Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Jan. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. iLID-GFP-GCN4-ppKin14

   Plasmid

   #240420

   Purpose

   -end oriented kinesin for optogenetic pulling experiments

   Depositor

   Anna Akhmanova

   Article

   Pasolli et al J Cell Biol. 2025 Sep 1;224(9):e20250400

   Insert

   ppKin14Vib
   

   Use

   Tags

   GCN4, GFP, and iLID

   Expression

   Mammalian

   Mutation

   deleted amino acids 1-850

   Promoter

   Available Since

   Aug. 8, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. HA-KIF5A-FRB

    Plasmid

    #240425

    Purpose

    +end oriented kinesin for rapalog induced pulling experiments.

    Depositor

    Anna Akhmanova

    Article

    Pasolli et al J Cell Biol. 2025 Sep 1;224(9):e20250400

    Insert

    KIF5A (Kif5a Mouse)
    

    Use

    Tags

    FRB and HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. Vim-mCh-FKBP

    Plasmid

    #240423

    Purpose

    Tagged vimentin for rapalog induced pulling experiments.

    Depositor

    Anna Akhmanova

    Article

    Pasolli et al J Cell Biol. 2025 Sep 1;224(9):e20250400

    Insert

    Vimentin (VIM Human)
    

    Use

    Tags

    FKBP and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. KIF5A-mVenus-iLID

    Plasmid

    #240422

    Purpose

    +end oriented kinesin for optogenetic pulling experiments

    Depositor

    Anna Akhmanova

    Article

    Pasolli et al J Cell Biol. 2025 Sep 1;224(9):e20250400

    Insert

    KIF5A (Kif5a Mouse)
    

    Use

    Tags

    iLID and mVenus

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. Vim-mCh-SspB

    Plasmid

    #240421

    Purpose

    Tagged vimentin for optogenetic vimentin pulling experiments.

    Depositor

    Anna Akhmanova

    Article

    Pasolli et al J Cell Biol. 2025 Sep 1;224(9):e20250400

    Insert

    Vimentin (VIM Human)
    

    Use

    Tags

    SspB and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pPB-cT3G-cERP2-RUNX1

    Plasmid

    #192928

    Purpose

    Barcoded piggybac transposon vector with Dox-inducible expression of RUNX1

    Depositor

    George Church

    Article

    Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

    Insert

    RUNX1 (RUNX1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TET3G-miniCMV

    Available Since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEGFP-C1-G3BP1-WT

    Plasmid

    #135997

    Purpose

    WT G3BP1 inserted with GFP tagged on the N-terminus used to stably integrate into cells

    Depositor

    Anthony Leung

    Article

    Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

    Insert

    G3BP1 (G3BP1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. EGFP-Bak

    Plasmid

    #32564

    Depositor

    Richard Youle

    Article

    Nechushtan et al J Cell Biol. 2001 Jun 11. 153(6):1265-76

    Insert

    human Bak (BAK1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 29, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA3/er-(n2)oxStayGold(c4)v2.0

    Plasmid

    #186296

    Purpose

    A new version of er-(n2)oxStayGold(c4), which gives a higher expression level. The StayGold gene has codon optimization for mammalian expression. For brighter ER imaging if permitted.

    Depositor

    Atsushi Miyawaki

    Article

    Hirano et al Nat Biotechnol. 2022 Apr 25. pii: 10.103

    Insert

    er-(n2)oxStayGold(c4)v2.0
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. OpenCRISPR-1 sgRNA-12nt stem

    Plasmid

    #221567

    Purpose

    Express OpenCRISPR-1 sgRNA (12nt stem loop) in human cells using a U6 promoter. Plasmid also encodes a CMV-driven GFP reporter.

    Depositor

    Ali Madani

    Article

    Ruffolo et al Nature. 2025 Jul 30. doi: 10.1038/s41586

    Insert

    OpenCRISPR-1 sgRNA 12nt stem loop
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Aug. 28, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. mCherry-Sec61β

    Plasmid

    #172445

    Purpose

    Expression of ER protein Sec61beta with an N-terminal mCherry tag

    Depositor

    Stephen Royle

    Article

    Ferrandiz et al J Cell Biol. 2022 Jun 6;221(6). pii: 213

    Insert

    Protein transport protein Sec61 subunit beta
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pscAAV-CAG-GFP

    Plasmid

    #83279

    Purpose

    recombinant AAV vector packaging self-complementary GFP under the CAG promoter

    Depositor

    Mark Kay

    Article

    Pekrun et al JCI Insight. 2019 Nov 14;4(22). pii: 131

    Insert

    enhanced green fluorescent protein
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    Oct. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge