4,457 results
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Plasmid#159750PurposeEgg cell-specific promoter-controlled expression of 3×FLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target sequence (U6-26 promoter). Selection of transgenic seeds by YFP fluoresce.DepositorArticleInsertgRNA scaffold
UseCRISPRExpressionPlantAvailable SinceOct. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMDC32-Kar2-CFP-HDEL
Plasmid#96989PurposeExpress endoplasmic reticulum (ER) marker in plants.DepositorInsertKar2-CFP-HDEL
ExpressionPlantPromoter35SAvailable SinceJuly 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDGE1109
Plasmid#202773PurposeCRISPR-KO with TRE2 exonuclease co-expression in Arabidopsis thalianaDepositorInsertsFAST marker
pRPS5a:TREX2-zCas9_ttriple
ccdB cassette (BsaI-excisable)
UseCRISPRExpressionPlantAvailable SinceAug. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pYPQ146-ZmUbi-tRNA
Plasmid#158404PurposeGateway entry clone and Golden Gate recipient for pYPQ131-tRNA2.0 to pYPQ136-tRNA2.0; assembly of 6 gRNAsDepositorTypeEmpty backboneUseCRISPRExpressionPlantPromoterMaize ubiquitin 1Available SinceJuly 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
UtB2N7
Plasmid#161015Purposeubiquitously expressed nuclear localized tagBFP2DepositorInserttagBFP2
ExpressionPlantAvailable SinceDec. 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDGB3 omega1 P35S:MS2:VPR:Tnos - P35S:dCas9:EDLL:Tnos (GB2085)
Plasmid#160645PurposeModule for the expression of Ms2 protein fused to VPR and dCas9 fused to EDLLDepositorInsertP35s-Ms2:VPR-Tnos-35s-dCas9:EDLL-Tnos
UseSynthetic BiologyExpressionPlantMutationBsmBI sites removedPromoter35S x2Available SinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCk3_spacer
Plasmid#136073PurposeUse when making even level constructs (L2,...) if pCk3 position (position 3) has no construct assignedDepositorInsertOdd spacer
UseSynthetic BiologyExpressionPlantMutationBsaI/ SapI domesticatedAvailable SinceNov. 30, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pH-Ubi-miniSdd7-nCas9-PBE
Plasmid#204856PurposeFor cytosine base editing using miniSdd7 in monocotyledon plants (Agrobacterium-mediated transformation)DepositorInsertminiSdd7-nSpCas9(D10A)-UGI
UseCRISPRExpressionPlantMutationD10A in SpCas9PromoterZmUbi-1, OsU3, 35SAvailable SinceAug. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pYLCRISPR/Cas9pUbi-N
Plasmid#106331PurposeExpression of Cas9 in plants, ZmUbi promoter, Kanamycin selectionDepositorInsertCas9
UseCRISPRExpressionPlantMutationplant-codon optimized with higher GC contents (62…PromoterZmUbiAvailable SinceMay 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTRANS_212
Plasmid#91111PurposeTransformation, Type: T-DNA, Plant Selection: 2x35S:hpt II, Viral Replication: ToLCVDepositorTypeEmpty backboneExpressionPlantPromoter35SAvailable SinceSept. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB201
Plasmid#68592PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneExpressionPlantAvailable SinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGWT35S-RBCS1a(1–79)-TagRFP
Plasmid#182586PurposeTransient expression of RBCS1a(1–79)-TagRFP in plant cell (Plastid/Chloroplast)DepositorInsertRBCS1a(1–79)
TagsTagRFPExpressionPlantPromoterCaMV 35S promoterAvailable SinceMay 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB304
Plasmid#68632PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneTagsGUSExpressionPlantAvailable SinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pYPQ-dpcoCas9-SunTag
Plasmid#158410PurposeCRISPR-dpcoCas9 fused with SunTag recruiting VP64 activators for transcriptional activationDepositorInsertdpcoCas9-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-VP64-GB1-NOS-ter
UseCRISPRExpressionPlantAvailable SinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGWB412
Plasmid#74806PurposeGateway cloning compatible binary vector for N-terminal fusion with FLAG (CaMV35S promoter).DepositorTypeEmpty backboneExpressionPlantPromoterCaMV35SAvailable SinceOct. 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
pYPQ-dpcoCas9-Act3.0
Plasmid#158408PurposeCRISPR-Act3.0 system containing dpcoCas9-VP64 fusion protein, T2A linked MS2-SunTag fusion protein, and ScFv-sfGFP-2xTAD activator.DepositorInsertdpcoCas9-VP64-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter
UseCRISPRExpressionPlantAvailable SinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGWT35S-OEP7-mCherry
Plasmid#182587PurposeTransient expression of OEP7-mCherry in plant cell (Outer envelope membrane of plastid/chloroplast)DepositorInsertOEP7
TagsmCherryExpressionPlantPromoterCaMV 35S promoterAvailable SinceMay 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
dpYPQ230-D156R-ABE8
Plasmid#195569PurposedLbCas12a-D156R based adenine base editor, ecTadA8e fused to the N terminal of dLbCas12a-D156R with the linker of (GSAGSAAGSGEF)x3DepositorInsertecTadA8e-(GSAGSAAGSGEF)x3-dLbCas12a-D156R
UseCRISPR; Gateway compatible ectada8e-(gsagsaagsgef…Tags5' and 3' NLSExpressionPlantPromoterZmUBI1Available SinceMay 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMAD7-crRNA-2
Plasmid#170135PurposeFor crRNA expression in monocotyledons protoplastsDepositorTypeEmpty backboneUseCRISPRExpressionPlantAvailable SinceSept. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYPQ136B2.0
Plasmid#167160PurposeGolden Gate entry vector to express the 6th gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU3 promoterDepositorTypeEmpty backboneUseCRISPRExpressionPlantPromoterAtU3Available SinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
CD Ugi4
Plasmid#177196PurposeGreengate entry vector pGGC000 modified to contain bladderwort insulator 4DepositorInsertUtricularia gibba insulator 4
ExpressionBacterial and PlantAvailable SinceJune 28, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
pEC1.2/EC1.1_Cas9i_TagRFP
Plasmid#227249PurposeCRISPR plasmid with Cas9i expressed under egg cell promoter - TagRFP seed selectionDepositorTypeEmpty backboneUseCRISPRExpressionPlantPromoterpEC1.2/EC1.1Available SinceNov. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAGT10446
Plasmid#192353PurposeL0 MoClo-compatible GoldenGate-module of the 35S terminator (t35S) with stopDepositorInsertTTCG_Stop-t35S_CGCT
ExpressionPlantAvailable SinceNov. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNC-Cam1304-MCS
Plasmid#193387PurposeNimble cloning-compatible vector for plant ectopic expressionDepositorTypeEmpty backboneExpressionPlantAvailable SinceDec. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB236
Plasmid#68627PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneTagsTagRFPExpressionPlantPromoterMpEF1alpha promoterAvailable SinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pISV-DsRed1
Plasmid#171024PurposeAgrobacterium rhizogenes-mediated hairy root transformation of Lotus japonicus.DepositorTypeEmpty backboneExpressionPlantPromoter2X EN 35SAvailable SinceAug. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBW_0065
Plasmid#102817PurposepVec8 containing Sr22 geneDepositorAvailable SinceFeb. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMP117
Plasmid#207920PurposeGreenBraid destination vector pGBZ2 - PaqCI - Eco31I - spisPink- Eco31I - PaqCIDepositorTypeEmpty backboneExpressionPlantAvailable SinceNov. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKD013
Plasmid#207931PurposePremade plasma membrane marker, pUBQ10:PiP1;4:mTurquoise2:tHSP18.2M, for braidingDepositorTypeEmpty backboneExpressionPlantAvailable SinceNov. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pChimera
Plasmid#61476PurposeVector for conventional cloning that contains AtU6-26 promoter and sgRNA backboneDepositorInsertU6-26:sgRNA
UseCRISPRExpressionPlantPromoterU6-26Available SinceSept. 24, 2018AvailabilityAcademic Institutions and Nonprofits only