4,307 results
-
Plasmid#164723PurposeGateway entry clone (attL1 & attR5) A3A/Y130F-iSpyMacCas9-CBE_V01 for C-T base editingDepositorInsertA3A(Y130F)-ziSpyMac(D10A)-UGI)
UseCRISPR; Gateway compatible a3a/y130f-ispymaccas9-…TagsExpressionPlantMutationPromoterAvailable sinceAug. 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFRETvr-2in1-CC
Plasmid#105117PurposeFRET/FLIM analysis in plants (stable or transient transformation)DepositorTypeEmpty backboneUseTagsmVenus/tagRFPExpressionPlantMutationPromoterAvailable sinceFeb. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCBLsgRNA3
Plasmid#199729PurposeGolden Gate entry vector; 3rd gRNA under TaU3 promoterDepositorInsertTaU3-sgRNA3 scaffold
UseTagsExpressionPlantMutationPromoterAvailable sinceApril 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCBLsgRNA4
Plasmid#199730PurposeGolden Gate entry vector; 4th gRNA under ZmU6-C1 promoterDepositorInsertZmU6C1-sgRNA4 scaffold
UseTagsExpressionPlantMutationPromoterAvailable sinceApril 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCBLsgRNA5
Plasmid#199731PurposeGolden Gate entry vector; 5th gRNA under ZmU6-C3 promoterDepositorInsertZmU6C3-sgRNA5 scaffold
UseTagsExpressionPlantMutationPromoterAvailable sinceApril 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCBLsgRNA6
Plasmid#199732PurposeGolden Gate entry vector; 6th gRNA under OsU6.1 promoterDepositorInsertOsU6.1-sgRNA6 scaffold
UseTagsExpressionPlantMutationPromoterAvailable sinceApril 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCBLsgRNA7
Plasmid#199733PurposeGolden Gate entry vector; 7th gRNA under OsU6.2 promoterDepositorInsertOsU6.2-sgRNA7 scaffold
UseTagsExpressionPlantMutationPromoterAvailable sinceApril 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCBLsgRNA8
Plasmid#199734PurposeGolden Gate entry vector; 8th gRNA under TaU6 promoterDepositorInsertTaU6-sgRNA8 scaffold
UseTagsExpressionPlantMutationPromoterAvailable sinceApril 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMC-1-AtUbi10-6
Plasmid#176871PurposeMoClo Level 0 plasmid, contains AtUbi1 promoterDepositorInsertAtUbi10
UseTagsExpressionPlantMutationWTPromoterAvailable sinceMay 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDGB3_alpha1
Plasmid#68228PurposeGoldenBraid level 1 Alpha plant expression vector.DepositorInsertempty vector
UseSynthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceMarch 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB311
Plasmid#68639PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneUseTags3xFLAGExpressionPlantMutationPromoterCauliflower mosaic virus 35S promoterAvailable sinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGWB401NL3F10H
Plasmid#141285PurposeNo promoter, C-terminal NLUC:3xFlag:10xHis tag, attR1-Cmr-ccdB-attR2-NLUC:3xFlag:3xHis-TNOS (modified pGWB401, pPZP backbone, SpcR for bacteria, KmR for plant)DepositorTypeEmpty backboneUseLuciferase; GatewayTagsFLAG (x3), HIS (x10), and NLUCExpressionPlantMutationPromoterAvailable sinceFeb. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJRA103
Plasmid#209336PurposeGateway cloning compatible binary vector for agrobacterium mediated transformation. Arabidopsis UBQ10 promoter expression of N-terminal SNAP-tag fusion protein.DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceJan. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB431
Plasmid#68696PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneUseTagsEmerald Luc fused with PEST sequence [ELuc(PEST)]ExpressionPlantMutationPromoterAvailable sinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGWB602-mCherry-sfGFP
Plasmid#213860PurposeFor Agrobacterium transformation. mCherry-sfGFP under the control of the cauliflower mosaic virus (CaMV) 35S promoter.DepositorInsertmCherry-sfGFP
UseTagsExpressionBacterial and PlantMutationPromoterCaMV 35SAvailable sinceMarch 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJG363
Plasmid#91183PurposeBeYDV replicon T-DNA for gene targeting in tobacco leaves, D10A single nickase (nAtCas9_D10A+gNt_R2+donor)DepositorInsertnAtCas9_D10A+gNt_R2+donor
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceSept. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJG367
Plasmid#91185PurposeBeYDV replicon T-DNA for gene targeting in tobacco leaves, H840A double nickase (nAtCas9_H840A+gNt_R2+gNt_F2+donor)DepositorInsertnAtCas9_H840A+gNt_R2+gNt_F2+donor
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceSept. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJG370
Plasmid#91187PurposeBeYDV replicon T-DNA for gene targeting in tobacco leaves, D10A single nickase no gRNA control (nAtCas9_D10A+gRNActrl+donor)DepositorInsertnAtCas9_D10A+gRNActrl+donor
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceSept. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJG382
Plasmid#91189PurposeBeYDV replicon T-DNA for gene targeting in tobacco leaves, D10A double nickase (nAtCas9_D10A+gNt_R2+gNt_F2+donor)DepositorInsertnAtCas9_D10A+gNt_R2+gNt_F2+donor
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceSept. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJET1.2-U6
Plasmid#173157PurposeSingle guide RNA cassette under U6 promoterDepositorInsertsgRNA cassette under U6 promoter
UseTagsExpressionPlantMutationPromoterAtU6-26Available sinceSept. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFGC-I2Cas9
Plasmid#173158PurposeExpresses Cas9 in dividing Arabidopsis cells. Contains dsRED and Basta for fast transformant selection.DepositorInsertshSpCas9
pNAP:dsRED:tNOS
pMAS:BAR:tMAS
ICU2 upstream regulatory region
UseTags3xFLAG-NLS and NLSExpressionPlantMutationContains a D169N mutation with no visible effect …PromoterAvailable sinceJune 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGG-NES-TurboID
Plasmid#209393PurposeTransiently expressing TurboID fused with a nuclear export signal (NES) in plantaDepositorInsertNES-3XHA-TurboID
UseTagsExpressionPlantMutationTurboID gene sequence C249APromoterCauliflower mosaic virus (CaMV) 35S promoterAvailable sinceJan. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGG-TurboID-GFP-PTS1
Plasmid#209406PurposeTransiently expressing and visualizing the subcellular localization of TurboID fused with a peroxisomal targeting signal type I (PTS1) in plantaDepositorInsert3XHA-TurboID-PTS1
UseTagsGFPExpressionPlantMutationTurboID gene sequence C249APromoterCauliflower mosaic virus (CaMV) 35S promoterAvailable sinceJan. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
JAB2098
Plasmid#190340PurposePlasmids encode parts for syntetic genetic circuit construction in eudicot plants (proUBQ10::PIP2A-mCherry-NOSt_pro35S-AmtRO-2XPhlFO::GFPint-ADHt)DepositorInsertproUBQ10::PIP2A-mCherry-NOSt_pro35S-AmtRO-2XPhlFO::GFPint-ADHt
UseTagsExpressionPlantMutationPromoterAvailable sinceApril 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pORFE0011
Plasmid#112075PurposepcUBI promoter module Pro+5UDepositorInsertpcUBI promoter
UseTagsExpressionPlantMutationBsaI and BpiI restriction sites have been remove…PromoterAvailable sinceAug. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
mTALEAD 16S rRNA-1139T10C
Plasmid#210838PurposemTALEAD expression vectorDepositorInsertFor specific A-to-G base editing to introduce targeted A-to-G mutation in 16S rRNA in chloroplast
UseTagsExpressionPlantMutationPromoterAvailable sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMP119
Plasmid#207949PurposeGreenBraid destination vector pGBY2 - Eco31I - PaqCI -sfGFP- PaqCI - Eco31IDepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceNov. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pdCas9 (GB1079)
Plasmid#75399PurposeProvides the human codon optimized CDS of Cas9 protein with mutated (D10A, H840A) and inactivated catalytic domains as a level 0 GoldenBraid part for C-terminal fusionsDepositorInsertCas9 coding region with mutated (D10A, H840A) and inactivated catalytic domains (human codon optimised)
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceAug. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
lvl0 UBQ10p
Plasmid#153363PurposePlant promoterDepositorInsertUBQ10 promoter
UseTagsExpressionPlantMutationPromoterAvailable sinceNov. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMOD_A5801
Plasmid#127223Purpose35S::LuciferaseDepositorInsertLuc
UseTagsExpressionPlantMutationcodon optimized CDSsPromoterAvailable sinceDec. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRW006-SM-destination-pro35S-Cas9
Plasmid#104439PurposeDestination vector expressing plant-codon-optimized Cas9 under 35S promoter, with sgRNAs be shuffled in; seed coat specific red fluorescence for screening trangene freeDepositorTypeEmpty backboneUseCRISPRTagsExpressionBacterial and PlantMutationPromoter35SAvailable sinceSept. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFGC5941-PacI-ath-STTM2937
Plasmid#84112Purposetarget miRNA2937 for destruction in Arabidopsis thalianaDepositorInsertSTTM2937
UseTagsExpressionPlantMutationPromoter2x35SAvailable sinceMarch 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSW725 - pUFO>>
Plasmid#116000Purposedestination vector for GreenGate cloning method, contains 2x a set of modules from A-F, "Driver line", tissues-specific promoter, Dex-inducible mTurquoise2 expressionDepositorInsertpUFO:GR-LhG4:tRBCS:F-H adapter-H-A adapter::pOp6:SP(ER)-mTurquoise2-HDEL:tUBQ10::SulfR
UseTagsHDEL and SP(ER)ExpressionPlantMutationPromoterAvailable sinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGWB528
Plasmid#74870PurposeGateway cloning compatible binary vector for C-terminal fusion with TAP (no promoter).DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceJuly 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFH113
Plasmid#128210Purposeimproved sgRNA backbone for tRNA-gRNA array, Position 1, combine with TaU3 promoter module (pFH31)DepositorInsertimproved sgRNA backbone for tRNA-gRNA array, Position 1, combine with TaU3 promoter module (pFH31)
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pORE303N_CEN12RRFuzzy3MYB
Plasmid#194441PurposeCRISPR, Cas9 driven by double 35S, nptII for plant selection, knockout: CEN12, RR, and Fuzzy3MYBDepositorInsertgRNA array targeting CEN1, RR, Fuzzy3MYB
UseCRISPRTagsExpressionPlantMutationPromoterMtU6Available sinceJan. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
CTAG_mScarlet-I
Plasmid#136118PurposeFP for C-term fusion with CDS12DepositorInsertCTAG-mScarletI(noATG)
UseSynthetic BiologyTagsExpressionPlantMutationBsaI/ SapI domesticatedPromoterAvailable sinceNov. 30, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
P303-FBP_2
Plasmid#139693PurposeT-DNA encoding Fungal Bioluminescent Pathway Components, complete pathway, without recycling gene CPHDepositorInsertp35S_Short_TMV 5':AsNpga:tAtug7, pCmYLCV:NnH3H:AtuOCS, pCmYLCV:NnHisps:tHsp, p35S:NnLuz:tAtuOcs
UseLuciferaseTagsExpressionPlantMutationPromotervariousAvailable sinceMay 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSB161 - pL2_pSB90_2x35S::GUS::tMAS
Plasmid#123197Purposebinary plant vector for transient expression of GUS (with introns)DepositorInsert2x35S::GUS::tMAS
UseTagsExpressionPlantMutationPromoterAvailable sinceMay 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDGE652
Plasmid#153230PurposeRecipient plant transformation vector containing selection markers and a Cas9 expression cassette, but lacking guide RNAsDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceSept. 9, 2020AvailabilityAcademic Institutions and Nonprofits only