4,457 results

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pDGB1alpha2_SulR

Plasmid

#186425

Purpose

transcriptional unit for sulfadiazine resistance; plant expression driven by the Pnos promoter

Depositor

Lukas Fischer

Article

Kobercova et al Plant Cell Rep. 2023 Jan 7. doi: 10.1007

Insert

SulR

Use

Synthetic Biology; Binary vector for escherichia …

Expression

Plant

Mutation

BsaI and BsmBI sites removed

Promoter

Pnos

Available Since

Jan. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pUPD1:TM6-MAR

Plasmid

#106215

Purpose

Provides N.tabacum TM6 matrix attachment region as a level 0 GoldenBraid part

Depositor

Tomáš Moravec

Insert

N.tabacum TM6 matrix attachment region

Use

Synthetic Biology

Expression

Plant

Available Since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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pCMB-ACTLg

Plasmid

#61195

Purpose

Fluorescent reporter for F-actin LifeAct-eGFP expressed under MtBCP1 promoter

Depositor

Maria Harrison

Article

Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

Insert

LifeAct-eGFP

Tags

eGFP

Expression

Plant

Promoter

MtBCP1

Available Since

March 30, 2015

Availability

Academic Institutions and Nonprofits only

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Raichu-OsRac1 CA

Plasmid

#133264

Purpose

Constitutively-active (CA) mutant of OsRac1; Acts as a positive control for small GTPases activation assays.

Depositor

Yoji Kawano

Article

Wong et al Plant Methods. 2018 Jul 7;14:56. doi: 10

Insert

OsRac1(G19V) (LOC4325879 Oryza sativa)

Tags

CFP-lipid and Venus-CRIB

Expression

Plant

Mutation

OsRac1 G19V

Promoter

Ubi

Available Since

Jan. 24, 2020

Availability

Academic Institutions and Nonprofits only

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Raichu-OsRac1 DN

Plasmid

#133263

Purpose

Dominant-negative (DN) mutant of OsRac1; Acts as a negative control for small GTPases activation assays

Depositor

Yoji Kawano

Article

Wong et al Plant Methods. 2018 Jul 7;14:56. doi: 10

Insert

OsRac1(T24N) (LOC4325879 Oryza sativa)

Tags

CFP-lipid and Venus-CRIB

Expression

Plant

Mutation

OsRac1 T24N

Promoter

Ubi

Available Since

Jan. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pICH-nuc-roGFP2-GPXC71S

Plasmid

#191760

Purpose

Ti plasmid pICH86988 harbouring nuc-roGFP2-GPXC71S -a nuclear-targeted control roGFP2 fusion not responsive to peroxides used alongside plasmids 191759 and 191758

Depositor

Philip Mullineaux

Article

Exposito-Rodriguez et al bioRxiv 2024.01.18.576236

Insert

nuc-roGFP2-GPXC71S

Use

Binary agrobacterium ti plasmid

Expression

Plant

Mutation

GPX has no peroxidatic Cysteine 71, which is repl…

Promoter

CaMV 35S

Available Since

March 10, 2023

Availability

Academic Institutions and Nonprofits only

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pMpGWB227

Plasmid

#68618

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Tags

TagRFP

Expression

Plant

Promoter

MpEF1alpha promoter

Available Since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

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pMpGWB106-MTS-Citrine

Plasmid

#79135

Purpose

Binary vectors for expression of Citrine fluorescent protein with mitochondria localization signal in plants

Depositor

Yutaka Kodama

Article

Ogasawara et al Plant Cell Environ. 2013 Feb 20. doi: 10

Insert

SD3(1-50)

Tags

Citrine

Expression

Plant

Promoter

CaMV 35S promoter

Available Since

Sept. 14, 2016

Availability

Academic Institutions and Nonprofits only

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pGEL029

Plasmid

#137898

Purpose

STU2.0 SpyCas9 RZ system for plant genome editing

Depositor

Yong Zhang

Article

Tang et al Plant Biotechnol J. 2019 Jul;17(7):1431-

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

Feb. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pMC-1-EXOB-11

Plasmid

#197688

Purpose

Phytobrick (MoClo) Level 0 Part

Depositor

Daniel Voytas

Article

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Insert

EXOB enhancer-blocking insulator

Expression

Plant

Available Since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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pDGE670

Plasmid

#153236

Purpose

Recipient plant transformation vector containing selection markers and a Cas9 expression cassette, but lacking guide RNAs

Depositor

Sylvestre Marillonnet

Article

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pGWB421

Plasmid

#74815

Purpose

Gateway cloning compatible binary vector for N-terminal fusion with 10xMyc (CaMV35S promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Promoter

CaMV35S

Available Since

Oct. 18, 2016

Availability

Academic Institutions and Nonprofits only

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pUBQ10::ZF108_3xFLAG_YPet

Plasmid

#106441

Purpose

pUBQ10 driven Zinc Finger YPet fusion that targets the FWA promoter in arabidopsis

Depositor

Steven Jacobsen

Article

Gallego-Bartolome et al Proc Natl Acad Sci U S A. 2018 Feb 27;11

Insert

ZF108_3xFLAG_YPet

Use

Synthetic Biology

Expression

Plant

Available Since

March 14, 2018

Availability

Academic Institutions and Nonprofits only

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pFLIPglu-600uDelta13

Plasmid

#12991

Depositor

Wolf Frommer

Article

Deuschle et al Plant Cell. 2006 Sep . 18(9):2314-25.

Insert

FLIPglu F16A Delta13

Use

Plant nanosensor

Tags

ECFP and EYFP

Expression

Plant

Mutation

F16A in binding protein

Available Since

Jan. 5, 2007

Availability

Academic Institutions and Nonprofits only
pHub10-His-YFP-PIP2;1

Plasmid

#99386

Purpose

overexpression and isolation of recombinant At-PIP2;1 protein

Depositor

Ning Li

Article

Qing et al Mol Plant. 2016 Jan 4;9(1):158-74. doi:

Insert

PIP2;1 (PIP2A Mustard Weed)

Tags

His and YFP

Expression

Plant

Promoter

2xCaMV35S

Available Since

Aug. 31, 2017

Availability

Academic Institutions and Nonprofits only

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UBQ10:sXVE:(MCS)-3xHA-S11

Plasmid

#108180

Purpose

Binary vectors used for the inducible expression of tripartite split-sfGFP components (with a 3xHA tag) in plants

Depositor

Tzu-Yin Liu

Article

Liu et al Plant J. 2018 Feb 16. doi: 10.1111/tpj.1

Type

Empty backbone

Use

Synthetic Biology

Expression

Plant

Promoter

UBQ10:sXVE:

Available Since

April 13, 2018

Availability

Academic Institutions and Nonprofits only

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pGWT35S-Cerulean-SKL

Plasmid

#194048

Purpose

Transient expression of Cerulean-SKL in plant cell (Peroxisome)

Depositor

Yutaka Kodama

Article

Ichikawa et al ACS Synth Biol. 2022 Oct 21;11(10):3190-

Insert

Cerulean

Tags

Ser-Lsy-Leu

Expression

Plant

Promoter

CaMV 35S promoter

Available Since

Jan. 19, 2023

Availability

Academic Institutions and Nonprofits only

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pTX209

Plasmid

#89269

Purpose

Express STU Cas9 with Maize Ubiquitin1 promoter in rice targeting OsPDS gene, OsPDS-gRNA01 and OsPDS-gRNA02

Depositor

Daniel Voytas

Article

Tang et al Mol Plant. 2016 Jul 6;9(7):1088-91. doi:

Insert

OsPDS-gRNA01 and OsPDS-gRNA02

Use

CRISPR

Tags

SV40 NLS

Expression

Plant

Promoter

Maize Ubiquitin1 promoter

Available Since

May 8, 2017

Availability

Academic Institutions and Nonprofits only

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UBQ10:sXVE:S10-(MCS)-3xHA

Plasmid

#108178

Purpose

Binary vectors used for the inducible expression of tripartite split-sfGFP components (with a 3xHA tag) in plants

Depositor

Tzu-Yin Liu

Article

Liu et al Plant J. 2018 Feb 16. doi: 10.1111/tpj.1

Type

Empty backbone

Use

Synthetic Biology

Expression

Plant

Promoter

UBQ10:sXVE:

Available Since

May 3, 2018

Availability

Academic Institutions and Nonprofits only

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pTX179

Plasmid

#89261

Purpose

Express STU (Single Transcriptional Unit) Cas9 nickase with Maize Ubiquitin1 promoter in plants

Depositor

Daniel Voytas

Article

Tang et al Mol Plant. 2016 Jul 6;9(7):1088-91. doi:

Type

Empty backbone

Use

CRISPR

Tags

SV40 NLS

Expression

Plant

Available Since

May 5, 2017

Availability

Academic Institutions and Nonprofits only

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SPDK3181

Plasmid

#97403

Purpose

binary construct of ER-targeted sfCherry1-10 for plant expression

Depositor

Savithramma Dinesh-Kumar

Article

Park et al Plant Cell. 2017 Jul;29(7):1571-1584. do

Insert

ER-sfCherry1-10

Tags

ER target

Expression

Plant

Available Since

Sept. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pYPQ141-ZmUbi-RZ-Cas12j2

Plasmid

#173926

Purpose

Gateway entry clone (attL5 & attL2) for Cas12j2 crRNA expression under ZmUbi promoter with ribozyme processing

Depositor

Yiping Qi

Article

Liu et al Plant Commun. 2022 Sep 20:100453. doi: 1

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pCLV3Cas-LT0

Plasmid

#210548

Purpose

Binary vector expressing the Lineage Tracing system. Cas9 expression driven by CLV3 promoter. LT0 reporter

Depositor

Ortrun Mittelsten Scheid

Article

Dona et al Plant J. 2023 Jul 5. doi: 10.1111/tpj.16

Insert

CLV3p::spCas9::PEA3At::U6p::AAVS1gRNA::pHTR5::LT0::mCherrySSM

Expression

Plant

Available Since

April 22, 2024

Availability

Academic Institutions and Nonprofits only

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LT0

Plasmid

#210551

Purpose

Binary vector expressing the Lineage Tracing system. No Cas9 control expression cassette. LT0 reporter

Depositor

Ortrun Mittelsten Scheid

Article

Dona et al Plant J. 2023 Jul 5. doi: 10.1111/tpj.16

Insert

pU6::AAVS1gRNA::LT0::mCherrySSM

Expression

Plant

Available Since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

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LT1

Plasmid

#210552

Purpose

Binary vector expressing the Lineage Tracing system. No Cas9 control expression cassette. LT1 reporter

Depositor

Ortrun Mittelsten Scheid

Article

Dona et al Plant J. 2023 Jul 5. doi: 10.1111/tpj.16

Insert

pU6::AAVS1gRNA::LT1::mCherrySSM

Expression

Plant

Available Since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

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LT2

Plasmid

#210553

Purpose

Binary vector expressing the Lineage Tracing system. No Cas9 control expression cassette. LT2 reporter

Depositor

Ortrun Mittelsten Scheid

Article

Dona et al Plant J. 2023 Jul 5. doi: 10.1111/tpj.16

Insert

pU6::AAVS1gRNA::LT2::mCherrySSM

Expression

Plant

Available Since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

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lvl0 G7t

Plasmid

#153380

Purpose

Agrobacterium terminator for plant expression

Depositor

Naomi Nakayama

Article

Andreou et al bioRxiv, March 31, 2021

Insert

G7 terminator

Expression

Plant

Available Since

Oct. 21, 2020

Availability

Academic Institutions and Nonprofits only

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P16ΔS:GFP1–9

Plasmid

#108186

Purpose

Binary vectors used for the constitutive expression of tripartite split-sfGFP components (GFP1-9) in plants

Depositor

Tzu-Yin Liu

Article

Liu et al Plant J. 2018 Feb 16. doi: 10.1111/tpj.1

Insert

GFP1-9

Use

Synthetic Biology

Expression

Plant

Promoter

P16ΔS

Available Since

May 3, 2018

Availability

Academic Institutions and Nonprofits only

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JAB2296

Plasmid

#190365

Purpose

Plasmids encode parts for syntetic genetic circuit construction in eudicot plants (proUBQ10::PIP2A-mCherry-NOSt_pro35S-PhlFO::GFPint-NLS-ADHt)

Depositor

Jennifer Brophy, Jose Dinneny

Article

Brophy et al Science. 2022 Aug 12;377(6607):747-751.

Insert

proUBQ10::PIP2A-mCherry-NOSt_pro35S-PhlFO::GFPint-NLS-ADHt

Expression

Plant

Available Since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

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JAB2121

Plasmid

#190343

Purpose

Plasmids encode parts for syntetic genetic circuit construction in eudicot plants (empty vector control)

Depositor

Jennifer Brophy, Jose Dinneny

Article

Brophy et al Science. 2022 Aug 12;377(6607):747-751.

Type

Empty backbone

Expression

Plant

Available Since

March 9, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge

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