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Plasmid#194344PurposesgRNA position entry clone 2DepositorInsertsgRNA expression cassettes and LacZ
UseTagsExpressionPlantMutationPromoterAth U6 promoterAvailable sinceApril 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pECNUS_P1
Plasmid#194343PurposesgRNA position entry clone 1DepositorInsertsgRNA expression cassettes and LacZ
UseTagsExpressionPlantMutationPromoterAth U6 promoterAvailable sinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pECNUS_P3
Plasmid#194345PurposesgRNA position entry clone 3DepositorInsertsgRNA expression cassettes and LacZ
UseTagsExpressionPlantMutationPromoterAth U6 promoterAvailable sinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pECNUS_P4
Plasmid#194346PurposesgRNA position entry clone 4DepositorInsertsgRNA expression cassettes and LacZ
UseTagsExpressionPlantMutationPromoterAth U6 promoterAvailable sinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pECNUS_P5
Plasmid#194347PurposesgRNA position entry clone 5DepositorInsertsgRNA expression cassettes and LacZ
UseTagsExpressionPlantMutationPromoterAth U6 promoterAvailable sinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pECNUS_P6
Plasmid#194348PurposesgRNA position entry clone 6DepositorInsertsgRNA expression cassettes and LacZ
UseTagsExpressionPlantMutationPromoterAth U6 promoterAvailable sinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pECNUS_P7
Plasmid#194349PurposesgRNA position entry clone 7DepositorInsertsgRNA expression cassettes and LacZ
UseTagsExpressionPlantMutationPromoterAth U6 promoterAvailable sinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB326
Plasmid#68654PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneUseTagsTagRFPExpressionPlantMutationPromoterNo PromoterAvailable sinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGL198
Plasmid#180472PurposeFor expression of Cas9 and gRNAs, multiple gRNAs cloning vectorDepositorTypeEmpty backboneUseCRISPRTagsZmCas9ExpressionPlantMutationPromoterAvailable sinceApril 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYPQ141-ZmUbi-RZ-Aac.4
Plasmid#158406PurposeExpress single gRNA with Aac.4 scaffold (with four MS2 binding sites) under ZmUbi promoterDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterMaize ubiquitin 1Available sinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFH33
Plasmid#125885PurposeLevel0 Wheat TaU3 Pol III promoter with transcription Start-ADepositorInsertLevel0 Wheat TaU3 Pol III promoter with transcription Start-A
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFGC5941-PacI-ath-STTM159
Plasmid#84084Purposetarget miRNA159 for destruction in Arabidopsis thalianaDepositorInsertSTTM159
UseTagsExpressionPlantMutationPromoter2x35SAvailable sinceMarch 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
LLP737_L2pV1_1I_Act2_FRT_OCS_Act2_Flp_lacZ
Plasmid#192380PurposeTo test if the Act2 promoter can drive the Flp recomhinase in order to remove the OCS terminator and increase circuit output.DepositorInsertAct2::FRT-OCS-FRT::Rluc
UseSynthetic BiologyTagsPESTExpressionPlantMutationPromoterAvailable sinceNov. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYPQ-dAaCas12b-Act3.0
Plasmid#158413PurposeCRISPR-Act3.0 system containing dAaCas12b-VP64 fusion protein, T2A linked MS2-SunTag fusion protein, and ScFv-sfGFP-2xTAD activator.DepositorInsertdAaCas12b-VP64-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceJuly 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEGB Tnos:NptII:Pnos (GB0226)
Plasmid#68212PurposeTranscriptional unit for kanamycin resistance gene plant expressionDepositorInsertNptII
UseSynthetic BiologyTagsExpressionPlantMutationBsaI and BsmBI sites removedPromoterPnosAvailable sinceOct. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
PDF1::mCitrine-MBD
Plasmid#115222PurposeMicrotubule visualizationDepositorInsertMicrotubule binding domain
UseTagsmCitrineExpressionPlantMutationPromoterAvailable sinceApril 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUBQ10::ZF2CACTA1_3xFLAG_TET1cd
Plasmid#106433PurposepUBQ10 driven Zinc Finger human TET1 catalytic domain fusion that targets the CACTA1 promoter in arabidopsisDepositorInsertpUBQ10::ZF1CACTA1_3xFLAG_TET1cd (TET1 Synthetic, Human, Mustard Weed)
UseSynthetic BiologyTags3xFLAGExpressionPlantMutationPromoterAvailable sinceMarch 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pG20_MCS_blp
Plasmid#159710PurposepGREEN-based cloning construct for expression in plantaDepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceOct. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pG20_MCS_Hyg
Plasmid#159709PurposepGREEN-based cloning construct for expression in plantaDepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceOct. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
INCTbiosyn-p35S-GFP(rc)13
Plasmid#127526PurposePlasmid has a CaMV 35S promoter in a forward sequence orientation and an inverted EGFP coding sequence (reverse complement) flanked by attB and attP integrase 13 attachment sites.DepositorInsertEGFP reverse complement coding sequence flanked by attB/attP Integrase 13 attachment sites.
UseTagsExpressionPlantMutationPromoterAvailable sinceMay 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pORFE0005
Plasmid#112073PurposeAtDeadCAS9 D10A H840A without stop codon module CDS1nsDepositorInsertAtDeadCAS9 D10A H840A without stop codon
UseCRISPRTagsExpressionPlantMutationPlant codon optimized dCas9 D10A S. pyogenesPromoterAvailable sinceAug. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
PV225
Plasmid#132334PurposedCas9 plant transcriptional activator (AT-CBF1) linked constitutive expression cassette for Zea maysDepositorInsertdCas9-AT-CBF1 (CBF1 Cas9 (Streptococcus pyogenes); AT-CBF1 (Arabidopsis thaliana))
UseTagsAT-CBF1ExpressionPlantMutationCodon optimize for expression and stability in Ze…PromoterAvailable sinceOct. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
BCJJ180
Plasmid#117516PurposeCas9_4 Golden Gate Level 0DepositorInsertBsaI:AATG:Cas9_4:GCTT:BsaI
UseCRISPRTags3xFLAG and NLSExpressionPlantMutationPromoterAvailable sinceNov. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pYPQ284-v2
Plasmid#138119PurposeGateway entry plasmid (attL1 & attR5) expressing rice codon optimized Mb2Cas12a with D172R, N563R and K569R mutations, without promoterDepositorInsertMb2Cas12a v2
UseCRISPR; Gateway compatible cas12a entry cloneTagsNLSExpressionPlantMutationD172R, N563R and K569RPromoterAvailable sinceMarch 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
A3A-PBE-ΔUGI
Plasmid#119770PurposeConversion of C to T in wheat plants or monocotyledons protoplasts without uracil glycosylase inhibitor (UGI)DepositorInsertAPOBEC3A-nCas9-ΔUGI-NLS
UseTagsNLSExpressionPlantMutationnCas9 has a mutation D10A in wild type SpCas9Promotermaize Ubiquitin-1Available sinceDec. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAM-PAT-35S-GW-Luc
Plasmid#80678Purposebinary DEST vector for plant expressionDepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterPro35SAvailable sinceFeb. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pUM31dao
Plasmid#87242PurposePlastid expression cassette containing Schizosaccharomyces pombe D-amino acid oxidase gene. Provides dual positive (D-alanine) and negative (D-valine) selection in transplastomic plantsDepositorInsertD-amino oxidase
UseTagsExpressionPlantMutationPromoterplastid rrnAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGWB419
Plasmid#74813PurposeGateway cloning compatible binary vector for C-terminal fusion with 10xMyc (no promoter).DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceJuly 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pNC-Cam2304-35S
Plasmid#193385PurposeNimble cloning-compatible vector for plant ectopic expressionDepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTT135
Plasmid#207924PurposePremade UBI10 driven expression of LhG4 (pUBQ10:GR-LhG4:tHSP18.2M) for braidingDepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceNov. 17, 2023AvailabilityAcademic Institutions and Nonprofits only