4,441 results

1. pCHW101008

   Plasmid

   #222453

   Purpose

   Level 1, Position 5 containing CBS4-miniDFR::OsL5::NOS.

   Depositor

   Chih-Hang Wu

   Article

   Chiang et al bioRxiv 2024.03.23.586378

   Insert

   CBS4-miniDFR::OsL5::NOS
   

   Use

   Synthetic Biology

   Expression

   Plant

   Mutation

   no

   Available Since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEE391

   Plasmid

   #149281

   Purpose

   T-DNA encoding TRV2 with FT augmented gRNA targeting NbPDS3

   Depositor

   Daniel Voytas

   Article

   Ellison et al Nat Plants. 2020 Jun 1. pii: 10.1038/s41

   Insert

   p35S:TRV2_NbPDS3sgRNA_FT:tNos
   

   Expression

   Plant

   Promoter

   35S

   Available Since

   July 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSOMA-NLS

   Plasmid

   #118937

   Purpose

   Used to express the SOMA FRET sensor with a nuclear localization signal in Arabidopsis thaliana

   Depositor

   Patrick Krysan

   Article

   Zaman et al Plant J. 2018 Nov 16. doi: 10.1111/tpj.1

   Insert

   SOMA Sensor for Arabidopsis MAPK activity with Nuclear Localization Signal
   

   Expression

   Plant

   Available Since

   Feb. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMpGWB303

   Plasmid

   #68631

   Purpose

   Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

   Depositor

   Takayuki Kohchi

   Article

   Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

   Type

   Empty backbone

   Expression

   Plant

   Promoter

   MpEF1alpha promoter

   Available Since

   June 24, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGES201

   Plasmid

   #190197

   Purpose

   For CRISPR-Cas9 mediated gene editing in soybean, soybean elongation factor 1A promoter (pM4)-Cas9, GmU6-sgRNA, Basta selection

   Depositor

   Yuefeng Guan

   Article

   Bai et al Plant Biotechnol J. 2020 Mar;18(3):721-7

   Insert

   spCas9
   

   Use

   CRISPR

   Tags

   3xFlag

   Expression

   Plant

   Mutation

   plant-codon optimized

   Promoter

   Glycine max elongation factor 1A(pM4)

   Available Since

   Sept. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMB-ACTFr

   Plasmid

   #61192

   Purpose

   Fluorescent reporter for F-actin AtFim-ABD2-mCherry expressed under MtBCP1 promoter

   Depositor

   Maria Harrison

   Article

   Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

   Insert

   AtFimbrin1-ABD
   

   Tags

   mCherry

   Expression

   Plant

   Promoter

   MtBCP1

   Available Since

   Feb. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSOMA-T679A-NLS

   Plasmid

   #118938

   Purpose

   Used to express the T679A mutant form of the SOMA FRET sensor with a nuclear localization signal in Arabidopsis thaliana

   Depositor

   Patrick Krysan

   Article

   Zaman et al Plant J. 2018 Nov 16. doi: 10.1111/tpj.1

   Insert

   SOMA Sensor for Arabidopsis MAPK activity with T679A Mutation and Nuclear Localization Signal
   

   Expression

   Plant

   Available Since

   Feb. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCambia-PUP-IT

   Plasmid

   #186478

   Purpose

   Expresses POI-PUP-IT fusion in Plants

   Depositor

   Jen Sheen

   Article

   Ye et al Nature. 2022 Sep 14. pii: 10.1038/s41586

   Insert

   FLAG-Pup(E)
   

   Tags

   Triple Flag Tag

   Expression

   Plant

   Promoter

   35S

   Available Since

   Sept. 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBiFCt-2in1-CC

   Plasmid

   #105114

   Purpose

   ratiometric BiFC analysis in plants (transient transformation)

   Depositor

   Christopher Grefen

   Article

   Grefen et al Biotechniques. 2012 Oct 2;53(5):311-14.

   Type

   Empty backbone

   Tags

   cYFP-Myc/nYFP-HA

   Expression

   Plant

   Available Since

   Feb. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJPC062

    Plasmid

    #234534

    Purpose

    TRV2 expressing CinH

    Depositor

    Daniel Voytas

    Article

    Chamness et al Plant Physiol. 2025 Mar 1;197(3):kiaf073

    Insert

    CinH Recombinase
    

    Use

    Recombinase

    Expression

    Plant

    Promoter

    CaMV 35S

    Available Since

    April 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJPC430

    Plasmid

    #234529

    Purpose

    CinH Recombinase switch; plant T-DNA

    Depositor

    Daniel Voytas

    Article

    Chamness et al Plant Physiol. 2025 Mar 1;197(3):kiaf073

    Insert

    RUBY
    

    Expression

    Plant

    Mutation

    none

    Available Since

    April 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. R4pGWB601_UBQ10p-Turbo-YFP-NLS

    Plasmid

    #127368

    Purpose

    Binary vector for expressing nuclear TurboID-YFP under the UBQ10 promoter in plants

    Depositor

    Dominique Bergmann

    Article

    Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

    Insert

    TurboID (BirA mutant)
    

    Tags

    GS linker, NLS, V5, and YFP

    Expression

    Plant

    Mutation

    Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

    Promoter

    Arabidopsis UBQ10 promoter

    Available Since

    Sept. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pJPC263

    Plasmid

    #234530

    Purpose

    Cre/Lox P1 Recombinase switch; plant T-DNA

    Depositor

    Daniel Voytas

    Article

    Chamness et al Plant Physiol. 2025 Mar 1;197(3):kiaf073

    Insert

    RUBY
    

    Expression

    Plant

    Mutation

    none

    Available Since

    April 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. ePPE-spG

    Plasmid

    #183096

    Purpose

    For plant prime editing in rice plants or monocotyledons protoplasts

    Depositor

    Caixia Gao

    Article

    Zong et al Nat Biotechnol. 2022 Mar 24. doi: 10.103

    Insert

    Nls-nspGCas9-NC-nls-MLV-Nls
    

    Use

    CRISPR

    Expression

    Plant

    Mutation

    D10A in SpGCas9

    Available Since

    April 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGreen2x35SLUC

    Plasmid

    #108228

    Purpose

    Plant expression as translational fusions to FLUC with RLUC normalization

    Depositor

    Alejandro Ferrando

    Article

    Solé-Gil et al Methods Mol Biol. 2024;2722:79-87. doi:

    Type

    Empty backbone

    Use

    Luciferase

    Tags

    Firefly Luciferase

    Expression

    Plant

    Promoter

    2x35S

    Available Since

    July 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pYPQ202

    Plasmid

    #86198

    Purpose

    Multisite Gateway T-DNA entry plasmid (attR1 & attR2); AtUBQ10 promoter and Hygromiycin resistance marker

    Depositor

    Yiping Qi

    Article

    Tang et al Nat Plants. 2017 Feb 17;3:17018. doi: 10

    Type

    Empty backbone

    Use

    Gateway compatible attr1-attr2 destination vector

    Expression

    Plant

    Promoter

    Arabidopsis ubiquitin 10 (AtUbi10)

    Available Since

    May 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. 35S-eGFP-nosT

    Plasmid

    #80127

    Purpose

    Transient expression vector for eGFP in plants

    Depositor

    Yutaka Kodama

    Article

    Fujii et al Plant Biotech. 2015; 32(1): 81-87.

    Type

    Empty backbone

    Tags

    eGFP

    Expression

    Plant

    Promoter

    CaMV35S

    Available Since

    Dec. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGWB433

    Plasmid

    #74824

    Purpose

    Gateway cloning compatible binary vector for C-terminal fusion with GUS (no promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Expression

    Plant

    Available Since

    July 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pYPQ131-tRNA2.0

    Plasmid

    #158393

    Purpose

    Golden Gate entry vector to express the 1st gRNA with tRNA2.0 scaffold (with four MS2 binding sites) without promoter

    Depositor

    Yiping Qi

    Article

    Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Plant

    Promoter

    Without promoter

    Available Since

    June 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGWB455

    Plasmid

    #74837

    Purpose

    Gateway cloning compatible binary vector for N-terminal fusion with mRFP (CaMV35S promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Expression

    Plant

    Promoter

    CaMV35S

    Available Since

    Sept. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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