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4,550 results

Showing: 161 - 180 of 4550 results

  3. pHUE411

    Plasmid
    #62203
    Purpose
    CRISPR/Cas based plant genome editing and gene regulation; expresses 3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence (OsU3 promoter), Hyg resistance
    Depositor
    Qi-Jun Chen
    Article
    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.
    Inserts
    gRNA scaffold
    zCas9
    Use
    Plant binary vector
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Mutation
    maize codon optimized
    Promoter
    OsU3 and Ubi
    Available Since
    April 29, 2015
    Availability
    Academic Institutions and Nonprofits only
    62203-plasmid-map-sequence-id-467234

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  4. pYPQAT15

    Plasmid
    #223387
    Purpose
    T-DNA vector for A3A/Y130F-CBE_V01 based C-to-T base editing for dicot plants; NGG PAM; wide working window; A3A/Y130F-CBE_V01 was driven by 2x35s and the sgRNA was driven by AtU3 promoter.
    Depositor
    Yiping Qi
    Article
    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.
    Insert
    2x35s-hA3A-Y130F-SpCas9-D10A-UGI-AtU3-gRNA scaffold
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Available Since
    April 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    223387-plasmid-map-sequence-id-452296

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  19. pYPQ-dpcoCas9-Act3.0

    Plasmid
    #158408
    Purpose
    CRISPR-Act3.0 system containing dpcoCas9-VP64 fusion protein, T2A linked MS2-SunTag fusion protein, and ScFv-sfGFP-2xTAD activator.
    Depositor
    Yiping Qi
    Article
    Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:
    Insert
    dpcoCas9-VP64-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter
    Use
    CRISPR
    Expression
    Plant
    Available Since
    June 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    158408-plasmid-map-sequence-id-330919

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  20. pHRE

    Plasmid
    #134909
    Purpose
    Overexpression of recombinant proteins in plants thanks to efficient translational efficiency. Empty vector, clone ORF using Bsa1 sites. Test UTR combinations by swapping 5' and 3' UTRs.
    Depositor
    George Lomonossoff
    Article
    Peyret et al Plant Methods. 2019 Sep 18;15:108. doi:
    Type
    Empty backbone
    Use
    Synthetic Biology
    Expression
    Plant
    Promoter
    double 35S
    Available Since
    Dec. 19, 2019
    Availability
    Academic Institutions and Nonprofits only
    134909-plasmid-map-sequence-id-267026

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Showing: 161 - 180 of 4550 results