1,786 results

Showing: 1 - 20 of 1786 results

Results per page:

Sort results by:

pCFJ90 - Pmyo-2::mCherry::unc-54utr

Plasmid

#19327

Depositor

Erik Jorgensen

Article

Christian et al

Insert

Pmyo-2::mCherry

Expression

Worm

Available Since

Oct. 15, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
L4440

Plasmid

#1654

Depositor

Andrew Fire

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

RNAi

Tags

T7p, T7p, lacZN, OriF1>>, OriF1<<

Expression

Worm

Available Since

March 7, 2005

Availability

Academic Institutions and Nonprofits only

Enlarge
pCFJ104 - Pmyo-3::mCherry::unc-54

Plasmid

#19328

Depositor

Erik Jorgensen

Article

Christian et al

Insert

Pmyo-3::mCherry

Tags

mCherry

Expression

Worm

Available Since

Nov. 21, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
pDD162 (Peft-3::Cas9 + Empty sgRNA)

Plasmid

#47549

Purpose

Cas9 + sgRNA plasmid that can be modified to cleave any Cas9 target site in the C. elegans genome.

Depositor

Bob Goldstein

Article

Dickinson et al Nat Methods. 2013 Sep 1. doi: 10.1038/nm

Inserts

Cas9
Empty sgRNA

Use

CRISPR

Tags

HA and NLS

Expression

Worm

Mutation

Codon optimized and with synthetic introns for C.…

Promoter

U6 and eef-1A.1 (eft-3)

Available Since

Sept. 9, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
hsp70l-loxP-mCherry-STOP-loxP-H2B-GFP_cryaa-cerulean

Plasmid

#24334

Depositor

Didier Stainier

Article

Hesselson et al Proc Natl Acad Sci U S A. 2009 Sep 1. 10

Insert

H2B

Use

Cre/Lox

Tags

GFP

Expression

Worm

Mutation

mCherry driven by an HSP70 promoterCerulean drive…

Available Since

April 27, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pPD95_75

Plasmid

#1494

Depositor

Andrew Fire

Type

Empty backbone

Tags

IVSA, gfpN, IVSR, gfpS65C, IVSS, IVST, gfpC, IVSL…

Expression

Worm

Available Since

March 7, 2005

Availability

Academic Institutions and Nonprofits only

Enlarge
pJN63 [pmyo-3::BeCyclOp::SL2::mCherry]

Plasmid

#168173

Purpose

Expression of BeCyclOp in BWMs of C. elegans

Depositor

Alexander Gottschalk

Article

Gao et al Nat Commun. 2015 Sep 8;6:8046. doi: 10.1

Insert

BeCyclOp (codon optimized for C. elegans)

Expression

Worm

Available Since

May 10, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
Pmyo-3::hpo-30 gDNA (pBAB204)

Plasmid

#117385

Purpose

Allows for body-wall muscle expression of HPO-30

Depositor

Kavita Babu

Article

Sharma et al J Neurosci. 2018 Aug 8;38(32):7072-7087.

Insert

genomic DNA of hpo-30 (833 bp)

Expression

Worm

Available Since

Feb. 8, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pDD268

Plasmid

#132523

Purpose

mNG^SEC^3xFlag vector with ccdB markers for cloning homology arms

Depositor

Daniel Dickinson, Bob Goldstein

Article

Dickinson et al Genetics. 2015 Jun 3. pii: genetics.115.

Insert

mNG-C1^SEC^3xFlag

Use

CRISPR and Cre/Lox

Tags

3xFlag and C. elegans codon-optimized mNG

Expression

Worm

Available Since

Oct. 31, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pMS114

Plasmid

#215677

Purpose

Empty repair plasmid for ChrI split hygromycinR landing pad

Depositor

Patrick Phillips

Article

Moerdyk-Schauwecker et al MicroPubl Biol. 2024 Jan 29;2024:10.1791

Insert

5'HA + MCS + loxN + rps-0p::5'ΔHygR

Use

CRISPR and Cre/Lox

Expression

Worm

Mutation

5' ∆HYGR encodes aa1-226

Available Since

March 21, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pMS59

Plasmid

#215680

Purpose

Cas9 + guide plasmid targeting ChrI split hygromycinR landing pad

Depositor

Patrick Phillips

Article

Moerdyk-Schauwecker et al MicroPubl Biol. 2024 Jan 29;2024:10.1791

Insert

eef-1A.1p::Cas9 + U6p::GTTTGAGTAGAGCACTCAGG

Use

CRISPR

Expression

Worm

Available Since

March 21, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pMS158

Plasmid

#215679

Purpose

Empty repair plasmid for ChrIII split hygromycinR landing pad

Depositor

Patrick Phillips

Article

Moerdyk-Schauwecker et al MicroPubl Biol. 2024 Jan 29;2024:10.1791

Insert

5'HA + MCS + lox2272 + rps-0p::5'ΔHygR

Use

CRISPR and Cre/Lox

Expression

Worm

Mutation

5' ∆HYGR encodes aa1-226

Available Since

March 21, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pMS77

Plasmid

#215681

Purpose

Cas9 + guide plasmid targeting ChrIII split hygromycinR landing pad

Depositor

Patrick Phillips

Article

Moerdyk-Schauwecker et al MicroPubl Biol. 2024 Jan 29;2024:10.1791

Insert

eef-1A.1p::Cas9 + U6p::GTCCAGCGGCAGATCGGCGG

Use

CRISPR

Expression

Worm

Available Since

March 21, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pSO221 Phis-72 BirA GFP

Plasmid

#45100

Depositor

Steven Henikoff

Article

Steiner et al Genome Res. 2012 Apr;22(4):766-77. doi:

Inserts

BirA::GFP
unc-119 (unc-119 Nematode)

Tags

GFP

Expression

Worm

Promoter

his-72 and unc-119

Available Since

June 4, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pWP095

Plasmid

#198789

Purpose

Co-expression plasmid for the CEP neurons

Depositor

Paul Sternberg

Article

Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

Insert

PF35D11.1-NLS-cGAL(DBD)-gp41-1-N-intein

Expression

Worm

Available Since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pWP097

Plasmid

#198790

Purpose

Co-expression plasmid for the CEP neurons

Depositor

Paul Sternberg

Article

Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

Insert

PF09E10.7-NLS-gp41-1-C-intein-cGAL(AD)

Expression

Worm

Available Since

April 14, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pSF11-wNIR-GECO2-T2A-HO1

Plasmid

#159606

Purpose

Codon-optimized near-infrared fluorescent calcium indicator NIR-GECO2 with codon-optimized heme-oxygenase in C.elegans expression plasmid

Depositor

Robert Campbell

Article

Qian et al PLoS Biol. 2020 Nov 24;18(11):e3000965.

Insert

wNIR-GECO2-T2A-HO1

Expression

Worm

Promoter

tag-168

Available Since

Sept. 22, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pdestMB14_sur5p-dCas9-VP64

Plasmid

#177788

Purpose

Overexpression of dCas9-VP64 in C. elegans

Depositor

Michael Ristow

Article

Fischer et al J Biol Chem. 2022 May 27:102085. doi: 10

Insert

dCas9-VP64

Expression

Worm

Available Since

June 1, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pGH8 - pRAB-3::mCherry::unc-54utr

Plasmid

#19359

Depositor

Erik Jorgensen

Article

Christian et al

Insert

Prab-3::mCherry

Tags

mCherry

Expression

Worm

Available Since

Oct. 29, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
pJW2098

Plasmid

#163094

Purpose

CRISPR repair template: add insertion site specific homology arms by PCR before insertion

Depositor

Jordan Ward

Article

Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

Insert

30aa linker::mScarlet (dpi)::AID*::3xFLAG::10aa linker

Use

CRISPR

Expression

Worm

Mutation

Silent mutations to remove piRNA sites

Available Since

Jan. 5, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge