1,779 results

1. pMS114

   Plasmid

   #215677

   Purpose

   Empty repair plasmid for ChrI split hygromycinR landing pad

   Depositor

   Patrick Phillips

   Article

   Moerdyk-Schauwecker et al MicroPubl Biol. 2024 Jan 29;2024:10.1791

   Insert

   5'HA + MCS + loxN + rps-0p::5'ΔHygR
   

   Use

   CRISPR and Cre/Lox

   Expression

   Worm

   Mutation

   5' ∆HYGR encodes aa1-226

   Available Since

   March 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMS59

   Plasmid

   #215680

   Purpose

   Cas9 + guide plasmid targeting ChrI split hygromycinR landing pad

   Depositor

   Patrick Phillips

   Article

   Moerdyk-Schauwecker et al MicroPubl Biol. 2024 Jan 29;2024:10.1791

   Insert

   eef-1A.1p::Cas9 + U6p::GTTTGAGTAGAGCACTCAGG
   

   Use

   CRISPR

   Expression

   Worm

   Available Since

   March 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMS158

   Plasmid

   #215679

   Purpose

   Empty repair plasmid for ChrIII split hygromycinR landing pad

   Depositor

   Patrick Phillips

   Article

   Moerdyk-Schauwecker et al MicroPubl Biol. 2024 Jan 29;2024:10.1791

   Insert

   5'HA + MCS + lox2272 + rps-0p::5'ΔHygR
   

   Use

   CRISPR and Cre/Lox

   Expression

   Worm

   Mutation

   5' ∆HYGR encodes aa1-226

   Available Since

   March 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMS77

   Plasmid

   #215681

   Purpose

   Cas9 + guide plasmid targeting ChrIII split hygromycinR landing pad

   Depositor

   Patrick Phillips

   Article

   Moerdyk-Schauwecker et al MicroPubl Biol. 2024 Jan 29;2024:10.1791

   Insert

   eef-1A.1p::Cas9 + U6p::GTCCAGCGGCAGATCGGCGG
   

   Use

   CRISPR

   Expression

   Worm

   Available Since

   March 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGLOW30

   Plasmid

   #172697

   Purpose

   C. elegans mScarlet co-injection marker

   Depositor

   Ryan Doonan

   Article

   Witten et al MicroPubl Biol. 2023 Jun 14;2023:10.1791

   Insert

   Pmyo-2::mScarlet
   

   Tags

   mScarlet

   Expression

   Worm

   Promoter

   myo-2

   Available Since

   Sept. 2, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. AID-mTagBFP2-loxP_myo2_neoR-loxP

   Plasmid

   #194055

   Purpose

   Dual-selection cassette plasmid for knocking-in AID-mTagBFP2 into the C. elegans genome

   Depositor

   Kota Mizumoto

   Article

   Kurashina et al STAR Protoc. 2023 Jan 13;4(1):102028. do

   Insert

   IAA17 (AXR3 Mustard Weed)
   

   Tags

   codon optimized mTagBFP2

   Expression

   Worm

   Promoter

   None

   Available Since

   Jan. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pWXC078

   Plasmid

   #222961

   Purpose

   C. elegans codon-optimized mScarlet-I3 with 3 introns cloned into pPD95.75 backbone

   Depositor

   Oliver Hobert

   Article

   Cao et al Genetics. 2024 Oct 7;228(2):iyae126. doi

   Insert

   mScarlet-I3
   

   Expression

   Worm

   Available Since

   July 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. 3xHA-TurboID_pAS31

   Plasmid

   #118220

   Purpose

   expresses 3xHA-tagged TurboID in C. elegans intestine

   Depositor

   Jessica Feldman

   Article

   Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

   Insert

   TurboID (BirA mutant)
   

   Tags

   3x HA tag

   Expression

   Worm

   Mutation

   Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

   Promoter

   ges-1p

   Available Since

   Jan. 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMDJ113 - pEXP(HygroR, 5605)[Phsp-16.41 |Cre recombinase (PATCs) | tbb-2 UTR]

   Plasmid

   #159809

   Purpose

   Optimized enzyme for genome editing in C. elegans

   Depositor

   Christian Froekjaer-Jensen

   Article

   Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

   Insert

   pEXP(HygroR, 5605)[Phsp-16.41 |Cre recombinase (PATCs) | tbb-2 UTR]
   

   Expression

   Worm

   Mutation

   Not applicable

   Available Since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJL43.1 - Pglh-2::MosTase::glh-2utr

    Plasmid

    #19332

    Depositor

    Erik Jorgensen

    Article

    Christian et al

    Insert

    Pglh-2::Mos1 transposase
    

    Expression

    Worm

    Available Since

    Oct. 15, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCZGY3196

    Plasmid

    #172696

    Purpose

    Prgef-1-gfp1-10 Expresses split GFP parts 1-10 in all C. elegans neurons (only shows active fluorescence when binds to GFP11)

    Depositor

    Yishi Jin

    Article

    Noma et al Elife. 2017 Aug 2;6. pii: e26376. doi: 1

    Insert

    Prgef-1
    

    Tags

    GFP 1-10

    Expression

    Worm

    Available Since

    Nov. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGLOW79

    Plasmid

    #177339

    Purpose

    C. elegans mNeonGreen co-injection marker

    Depositor

    Ryan Doonan

    Article

    Witten et al MicroPubl Biol. 2023 Jun 14;2023:10.1791

    Insert

    Pmyo-3::mNeonGreen
    

    Tags

    mNeonGreen

    Expression

    Worm

    Promoter

    myo-3

    Available Since

    Jan. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCZGY546

    Plasmid

    #90466

    Purpose

    Pmec-4::mcherry, Expresses mcherry red fluorescence in C. elegans touch neurons

    Depositor

    Andrew Chisholm

    Article

    Xu et al Sci Rep. 2016 Feb 10;6:21271. doi: 10.10

    Insert

    Pmec-4-mcherry
    

    Tags

    mcherry

    Expression

    Worm

    Promoter

    Pmec-4

    Available Since

    July 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCFJ90 - Pmyo-2::mCherry::unc-54utr

    Plasmid

    #19327

    Depositor

    Erik Jorgensen

    Article

    Christian et al

    Insert

    Pmyo-2::mCherry
    

    Expression

    Worm

    Available Since

    Oct. 15, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMDJ39 - Peft-3 Cre (PATCs)

    Plasmid

    #191381

    Purpose

    High efficiency CRE recombinase expression in C. elegans

    Depositor

    Christian Froekjaer-Jensen

    Article

    El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

    Insert

    Peft-3 Cre tbb-2 UTR
    

    Expression

    Worm

    Promoter

    Peft-3

    Available Since

    Nov. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCFJ104 - Pmyo-3::mCherry::unc-54

    Plasmid

    #19328

    Depositor

    Erik Jorgensen

    Article

    Christian et al

    Insert

    Pmyo-3::mCherry
    

    Tags

    mCherry

    Expression

    Worm

    Available Since

    Nov. 21, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. 3xHA-miniTurbo_pAS32

    Plasmid

    #118222

    Purpose

    expresses 3xHA-tagged miniTurbo in C. elegans intestine

    Depositor

    Jessica Feldman

    Article

    Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

    Insert

    miniTurbo (BirA mutant)
    

    Tags

    3x HA tag

    Expression

    Worm

    Mutation

    aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

    Promoter

    ges-1p

    Available Since

    Jan. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pJL049

    Plasmid

    #198809

    Purpose

    In vivo calcium indicator. Presence of calcium (Ca2+) increases reporter signal intensity.

    Depositor

    Paul Sternberg

    Article

    Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

    Insert

    15xUAS::GCaMP6s-SL2-mKate2::let-858 3'UTR
    

    Expression

    Worm

    Available Since

    May 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pJR022

    Plasmid

    #198820

    Purpose

    C. elegans optimized version of mScarlet

    Depositor

    Paul Sternberg

    Article

    Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

    Insert

    15xUAS::wrmScarlet::let-858 3'UTR
    

    Expression

    Worm

    Available Since

    April 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGH8 - pRAB-3::mCherry::unc-54utr

    Plasmid

    #19359

    Depositor

    Erik Jorgensen

    Article

    Christian et al

    Insert

    Prab-3::mCherry
    

    Tags

    mCherry

    Expression

    Worm

    Available Since

    Oct. 29, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge