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pSEM229 - [Pmlc-1 | mNeonGreen | cbr-tbb-2 UTR]

Plasmid

#159896

Purpose

Fluorescent co-injection marker to identify extrachromosomal arrays in C. elegans

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al MicroPubl Biol. 2020 Sep 3;2020. doi: 10

Insert

Pmlc-1 | mNeonGreen | cbr-tbb-2 UTR

Expression

Worm

Available Since

Oct. 29, 2020

Availability

Academic Institutions and Nonprofits only

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pSEM228 - [Pmlc-1 | mNeonGreen(2x NLS) | cbr-tbb-2 UTR]

Plasmid

#159895

Purpose

Fluorescent co-injection marker to identify extrachromosomal arrays in C. elegans

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al MicroPubl Biol. 2020 Sep 3;2020. doi: 10

Insert

Pmlc-1 | mNeonGreen(2x NLS) | cbr-tbb-2 UTR

Expression

Worm

Available Since

Oct. 29, 2020

Availability

Academic Institutions and Nonprofits only

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pJH3830

Plasmid

#200038

Purpose

Prig-3 zif-1 SL2 mCherry unc-54 3' UTR C.elegans AVA and other neurons expression of zif RFP

Depositor

Mei Zhen

Article

Meng et al Sci Adv. 2024 Apr 12;10(15):eadk0002. do

Insert

ZIF (zif-1 Nematode)

Tags

mCherry

Expression

Worm

Promoter

Prig-3

Available Since

May 7, 2024

Availability

Academic Institutions and Nonprofits only

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pWXC078

Plasmid

#222961

Purpose

C. elegans codon-optimized mScarlet-I3 with 3 introns cloned into pPD95.75 backbone

Depositor

Oliver Hobert

Article

Cao et al Genetics. 2024 Oct 7;228(2):iyae126. doi

Insert

mScarlet-I3

Expression

Worm

Available Since

July 31, 2024

Availability

Academic Institutions and Nonprofits only

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3xHA-TurboID_pAS31

Plasmid

#118220

Purpose

expresses 3xHA-tagged TurboID in C. elegans intestine

Depositor

Jessica Feldman

Article

Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

Insert

TurboID (BirA mutant)

Tags

3x HA tag

Expression

Worm

Mutation

Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

Promoter

ges-1p

Available Since

Jan. 14, 2019

Availability

Academic Institutions and Nonprofits only

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pCFJ2474 - pEXP[Psmu-2 | cas9(PATCs) | gpd-2 tagRFP-T(myr, patcs) smu-1 UTR]

Plasmid

#159816

Purpose

Optimized enzyme for genome editing in C. elegans

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

pEXP[Psmu-2 | cas9(PATCs) | gpd-2 tagRFP-T(myr, patcs) smu-1 UTR]

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pCFJ782 - HygroR

Plasmid

#190933

Purpose

Hygromycin resistance co-injection marker

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Insert

Hygromycin resistance gene

Expression

Worm

Promoter

rps-0

Available Since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pAttBFHyg2

Plasmid

#244927

Purpose

attB Hyg Resistant plasmid for RMI targeting an attP site

Depositor

Michael Nonet

Article

Nonet et al bioRxiv 2024.03.01.583017

Type

Empty backbone

Use

Phic31 recombinase mediated insertion vector

Expression

Worm

Promoter

none

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pSEM318 - sgRNA mosTI unc-119 single-copy

Plasmid

#159822

Purpose

Single copy or array insertion by MosTI using split unc-119 selection

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Insert

sgRNA mosTI unc-119 single-copy

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pDACR3882 [rab-3p::HYlight::let-858utr]

Plasmid

#207666

Purpose

Expresses worm-optimized HYlight in C. elegans neurons

Depositor

Daniel Colón-Ramos

Article

Wolfe et al Proc Natl Acad Sci U S A. 2024 Jan 16;12

Insert

HYlight Biosensor

Expression

Worm

Promoter

rab-3

Available Since

Feb. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pDACR3883 [rab-3p::HYlight-RA::let-858utr]

Plasmid

#207667

Purpose

Expresses HYlight-RA, the negative control HYlight variant with reduced binding affinity, in C. elegans neurons

Depositor

Daniel Colón-Ramos

Article

Wolfe et al Proc Natl Acad Sci U S A. 2024 Jan 16;12

Insert

HYlight-RA [T152E]

Expression

Worm

Promoter

rab-3

Available Since

Feb. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pSEM246 - mosTI - unc-119 - MCS

Plasmid

#182343

Purpose

Empty cloning vector for mosTI(unc-119). MCS or Golden Gate (BsaI: tgcc - MCS - gagc)

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Type

Empty backbone

Expression

Worm

Available Since

May 16, 2022

Availability

Academic Institutions and Nonprofits only

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pCZGY546

Plasmid

#90466

Purpose

Pmec-4::mcherry, Expresses mcherry red fluorescence in C. elegans touch neurons

Depositor

Andrew Chisholm

Article

Xu et al Sci Rep. 2016 Feb 10;6:21271. doi: 10.10

Insert

Pmec-4-mcherry

Tags

mcherry

Expression

Worm

Promoter

Pmec-4

Available Since

July 20, 2022

Availability

Academic Institutions and Nonprofits only

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TetR in pENTR1A (559-1)

Plasmid

#22265

Depositor

Eric Campeau

Insert

Tetracycline repressor (tetR )

Expression

Insect, Mammalian, Worm Exp…

Available Since

Dec. 17, 2009

Availability

Academic Institutions and Nonprofits only

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pHygO2r

Plasmid

#200079

Purpose

hygR selection unmarked rapid RMCE integration vector

Depositor

Michael Nonet

Article

Nonet et al Genetics. 2023 Apr 20:iyad072. doi: 10.1

Type

Empty backbone

Use

Recombination mediated cassette exchange

Expression

Worm

Available Since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

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pAttBFHyg

Plasmid

#244926

Purpose

attB Hyg Resistant plasmid for RMI targeting an attP site

Depositor

Michael Nonet

Article

Nonet et al bioRxiv 2024.03.01.583017

Type

Empty backbone

Use

Phic31 recombinase mediated insertion (rmi)

Expression

Worm

Promoter

none

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pWXC077

Plasmid

#222960

Purpose

C. elegans codon-optimized mScarlet3 with 3 introns cloned into pPD95.75 backbone

Depositor

Oliver Hobert

Article

Cao et al Genetics. 2024 Oct 7;228(2):iyae126. doi

Insert

mScarlet3

Expression

Worm

Available Since

July 31, 2024

Availability

Academic Institutions and Nonprofits only

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DR274

Plasmid

#42250

Purpose

guide RNA (gRNA) expression vector used to create a gRNA to a specific sequence; uses T7 promoter for in vitro transcription

Depositor

Keith Joung

Article

Hwang et al Nat Biotechnol. 2013 Jan 29. doi: 10.103

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

CRISPR; Zebrafish expression

Expression

Worm

Promoter

T7

Available Since

Jan. 31, 2013

Availability

Academic Institutions and Nonprofits only

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pHygCG2

Plasmid

#200073

Purpose

hygR selection mNG marked rapid RMCE integration vector

Depositor

Michael Nonet

Article

Nonet et al Genetics. 2023 Apr 20:iyad072. doi: 10.1

Type

Empty backbone

Use

Recombination mediated cassette exchange

Expression

Worm

Available Since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

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pZCS16 (Peft-3::wrmScarlet::tbb-2 3'UTR in pUC19)

Plasmid

#154824

Purpose

(eft-3p::wrmScarlet::tbb-2 3'UTR in pUC19) Experimentally used to mark formation of transgenic arrays for C. elegans

Depositor

Patrick Phillips

Article

Stevenson et al G3 (Bethesda). 2020 Aug 19. pii: g3.120.

Insert

Peft-3::wrmScarlet::tbb-2 UTR

Expression

Worm

Promoter

eef-1A.1 (eft-3)

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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