1,779 results

1. pSEM236 - pEXP[Pmlc-2 | histamine | rpl-3 UTR]

   Plasmid

   #159797

   Purpose

   Histamine based negative selection marker to paralyze animals with arrays

   Depositor

   Christian Froekjaer-Jensen

   Article

   El Mouridi et al MicroPubl Biol. 2021 Jan 8;2021. doi: 10

   Insert

   pEXP[Pmlc-2 | histamine | rpl-3 UTR]
   

   Expression

   Worm

   Mutation

   Not applicable

   Available Since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. XW17

   Plasmid

   #65835

   Purpose

   Punc-4-QF-3'UTR-SL2-mCherry-unc-54 (The endogenous SphI, KpnI and SacI sites in QF are removed for the convenience of cloning, most sites are compatible with pPD95.77)

   Depositor

   Kang Shen

   Article

   Wei et al Nat Methods. 2012 Mar 11;9(4):391-5. doi

   Insert

   OR74A quinic acid utilization activator (qa-1F)
   

   Tags

   mCherry

   Expression

   Worm

   Mutation

   The endogenous SphI, KpnI and SacI sites in QF ar…

   Available Since

   Sept. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. TU#712

   Plasmid

   #15280

   Depositor

   Martin Chalfie

   Article

   Zhang et al Cell. 2004 Oct 1. 119(1):137-44.

   Insert

   nzyfp
   

   Expression

   Worm

   Mutation

   S65G, V68L, S72A, and T203Y

   Available Since

   June 23, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRB1083

   Plasmid

   #71480

   Purpose

   Expresses VRER Cas9 variant with eef-1A.1 (eft-3) promoter

   Depositor

   Andrew Fire

   Article

   Bell et al Genetics. 2015 Dec 17. pii: genetics.115

   Insert

   VRER Cas9 variant
   

   Use

   CRISPR

   Expression

   Worm

   Promoter

   eft-3 (eef-1A.1)

   Available Since

   Dec. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJW1883

   Plasmid

   #154331

   Purpose

   sgRNA Target Vector for CRISPR

   Depositor

   Jordan Ward

   Article

   Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

   Insert

   ttTi5605 site targeting sgRNA (F+E) with R07E5.16 U6 promoter and 3'UTR
   

   Use

   CRISPR

   Expression

   Worm

   Mutation

   F+E mutations in sgRNA

   Available Since

   Dec. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBCN44-R4R3

   Plasmid

   #34919

   Depositor

   Ben Lehner

   Article

   Semple et al Nat Methods. 2012 Jan 30;9(2):118-9. doi

   Type

   Empty backbone

   Expression

   Worm

   Available Since

   March 21, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCC20

   Plasmid

   #198783

   Purpose

   Drives specific expression in the ASK neurons

   Depositor

   Paul Sternberg

   Article

   Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

   Insert

   Psra-9-GAL4-SK(DBD)-VP64
   

   Expression

   Worm

   Available Since

   April 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pNM3749

   Plasmid

   #154108

   Purpose

   loxP FRT FRT3 RMCE landing site in a Golden Gate SapI GCG ACG assembly vector

   Depositor

   Michael Nonet

   Article

   Nonet et al Genetics. 2020 Jun 8. pii: genetics.120.

   Inserts

   LoxP-SEC-LoxP
   FRT::rpl-28p::GFP::his-58::FRT3
   

   Use

   Cre/Lox; Rmce

   Expression

   Worm

   Promoter

   not applicable

   Available Since

   July 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBN303

   Plasmid

   #86730

   Purpose

   Tissue-specific FLP expression plasmid for C. elegans

   Depositor

   Peter Askjaer

   Article

   Munoz-Jimenez et al Genetics. 2017 Aug;206(4):1763-1778. doi

   Insert

   Punc-47::FLP D5
   

   Expression

   Worm

   Available Since

   April 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDD399

    Plasmid

    #91821

    Purpose

    AID CT-tag linker donor for the SapTrap cloning system

    Depositor

    Bob Goldstein

    Article

    Dickinson et al MicroPubl Biol. 2018 May 1;2018:10.17912

    Insert

    Auxin-inducible degron (AID)
    

    Use

    CRISPR

    Expression

    Worm

    Available Since

    June 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMLS235

    Plasmid

    #73683

    Purpose

    SapTrap 3-site Destination vector for C-terminal GFP tagging with embedded Cbr-unc-119 selectable marker

    Depositor

    Erik Jorgensen

    Article

    Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

    Insert

    GFP + Cbr-unc-119
    

    Use

    CRISPR and Cre/Lox

    Expression

    Worm

    Available Since

    April 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pPD95.77-wVenus

    Plasmid

    #37465

    Depositor

    Hitoshi Sawa

    Article

    Yamamoto et al PLoS Genet. 2011 Oct;7(10):e1002308. Epu

    Type

    Empty backbone

    Expression

    Worm

    Promoter

    none

    Available Since

    Aug. 17, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pJW1947

    Plasmid

    #154339

    Purpose

    Enhancer reporter

    Depositor

    Jordan Ward

    Article

    Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

    Insert

    pes-10delta::SV40 NLS::mScarlet_I(dpi)::-tbb-2 3'UTR
    

    Expression

    Worm

    Mutation

    Silent mutations to remove piRNA sites

    Available Since

    Aug. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCFJ2441 - [1-2] ENTR - Fluor - ce-Dendra2(PATCs(900bp), NLS, no_atg, no stop)

    Plasmid

    #159870

    Purpose

    Three-fragment Gateway compatible flourophore for expression in C. elegans

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    [1-2] ENTR - Fluor - ce-Dendra2(PATCs(900bp), NLS, no_atg, no stop)
    

    Expression

    Worm

    Mutation

    Not applicable

    Available Since

    Dec. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBN260

    Plasmid

    #86722

    Purpose

    Tissue-specific FLP expression plasmid for C. elegans

    Depositor

    Peter Askjaer

    Article

    Munoz-Jimenez et al Genetics. 2017 Aug;206(4):1763-1778. doi

    Insert

    Pmyo-3::FLP D5
    

    Expression

    Worm

    Available Since

    April 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. TU#713

    Plasmid

    #15281

    Depositor

    Martin Chalfie

    Article

    Zhang et al Cell. 2004 Oct 1. 119(1):137-44.

    Insert

    czyfp
    

    Expression

    Worm

    Available Since

    June 23, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. L4271

    Plasmid

    #1646

    Depositor

    Andrew Fire

    Type

    Empty backbone

    Use

    RNAi

    Tags

    T3p, gfpN, gfpS65S, gfpC, unc2217896, unc223827, …

    Expression

    Worm

    Available Since

    March 7, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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18. L4667

    Plasmid

    #1669

    Depositor

    Andrew Fire

    Type

    Empty backbone

    Tags

    myo3-2341, myo3Tsa, IVSA, rf2128, ATG-MitLS, gfpN…

    Expression

    Worm

    Available Since

    March 7, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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19. pRB1081

    Plasmid

    #71479

    Purpose

    Expresses gRNA for dpy-10 conversion using NGAG PAM and VQR Cas9 variant

    Depositor

    Andrew Fire

    Article

    Bell et al Genetics. 2015 Dec 17. pii: genetics.115

    Insert

    dpy-10 gRNA
    

    Use

    CRISPR

    Expression

    Worm

    Promoter

    U6

    Available Since

    Dec. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pPD93_48

    Plasmid

    #1473

    Depositor

    Andrew Fire

    Type

    Empty backbone

    Tags

    unc54-204, unc545', IVSA, SV40NLS, gfpN, IVS…

    Expression

    Worm

    Available Since

    March 7, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pJW1241

    Plasmid

    #74904

    Purpose

    Expresses 3XFLAG::GR LBD::eGFP from the hsp16.48 promoter

    Depositor

    Jordan Ward, Keith Yamamoto

    Article

    Monsalve et al Genetics. 2019 Feb;211(2):419-430. doi:

    Insert

    3X FLAG::GR LBD::eGFP (NR3C1 Nematode, Human, Synthetic)
    

    Expression

    Worm

    Promoter

    hsp16.48

    Available Since

    Oct. 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pNM3734

    Plasmid

    #154118

    Purpose

    mec-4 promoter driving GAL4-QFAD in an RCME vector

    Depositor

    Michael Nonet

    Article

    Nonet et al Genetics. 2020 Jun 8. pii: genetics.120.

    Insert

    mec-4p::Gal4SK::QFAD::tbb-2 '3
    

    Use

    Rmce

    Expression

    Worm

    Promoter

    not applicable

    Available Since

    July 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pBCN42-R4R3

    Plasmid

    #34917

    Depositor

    Ben Lehner

    Article

    Semple et al Nat Methods. 2012 Jan 30;9(2):118-9. doi

    Type

    Empty backbone

    Expression

    Worm

    Available Since

    March 21, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. L2534

    Plasmid

    #1608

    Depositor

    Andrew Fire

    Type

    Empty backbone

    Tags

    myo3-2341, myo3Tsa, IVSA, rf2128, IVSM, IVSL, dec…

    Expression

    Worm

    Available Since

    March 7, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pGM59

    Plasmid

    #74785

    Purpose

    Expresses 3XFLAG::GR LBD::DAF-16A from the hsp16.48 promoter

    Depositor

    Jordan Ward, Keith Yamamoto

    Article

    Monsalve et al Genetics. 2019 Feb;211(2):419-430. doi:

    Insert

    3X FLAG::GR LBD::DAF-16A (daf-16, NR3C1 Nematode, Human, Synthetic)
    

    Expression

    Worm

    Promoter

    hsp16.48

    Available Since

    Oct. 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pEY79

    Plasmid

    #191074

    Purpose

    flp-12 fluorescent neural reporter driving nuclear T-Sapphire expression (refer to NeuroPAL paper for expression)

    Depositor

    Oliver Hobert, Eviatar Yemini

    Article

    Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

    Insert

    flp-12 (flp-12 Nematode)
    

    Expression

    Worm

    Mutation

    None

    Available Since

    Nov. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pEY62

    Plasmid

    #191020

    Purpose

    acr-5 fluorescent neural reporter driving nuclear mTagBFP2 expression (refer to NeuroPAL paper for expression)

    Depositor

    Oliver Hobert, Eviatar Yemini

    Article

    Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

    Insert

    acr-5 (acr-5 Nematode)
    

    Expression

    Worm

    Mutation

    None

    Available Since

    Nov. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pCZGY2844

    Plasmid

    #87760

    Purpose

    Punc-17b-PH::miniSOG, expression of membrane targeted PH-miniSOG in cholinergic motor neurons.

    Depositor

    Andrew Chisholm

    Article

    Xu et al Sci Rep. 2016 Feb 10;6:21271. doi: 10.10

    Insert

    Punc-17b-PH-miniSOG
    

    Tags

    PH

    Expression

    Worm

    Promoter

    Punc-17b

    Available Since

    April 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pSR47

    Plasmid

    #69258

    Purpose

    pmyo-3::CRE for integration on ttTi14024, Chr X

    Depositor

    Sander van den Heuvel

    Article

    Ruijtenberg et al Cell. 2015 Jul 16;162(2):300-13. doi: 10

    Insert

    Cre recombinase
    

    Use

    Cre/Lox; Mossci

    Expression

    Worm

    Mutation

    codon-optimzed index 1.0

    Promoter

    myo-3

    Available Since

    Oct. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pBN283

    Plasmid

    #86728

    Purpose

    Tissue-specific FLP expression plasmid for C. elegans

    Depositor

    Peter Askjaer

    Article

    Munoz-Jimenez et al Genetics. 2017 Aug;206(4):1763-1778. doi

    Insert

    Pdat-1::FLP D5
    

    Expression

    Worm

    Available Since

    Feb. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge