1,779 results

1. pXF222

   Plasmid

   #139032

   Purpose

   MosSCI SapTrap assembly: Cassette 2 for 4- or 5-cassette system

   Depositor

   Erik Griffin

   Article

   Fan et al G3 (Bethesda). 2019 Dec 17. pii: g3.119.

   Insert

   mkate2 (no STOP codon)
   

   Expression

   Worm

   Available Since

   March 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEG412

   Plasmid

   #40087

   Depositor

   Geraldine Seydoux

   Article

   Griffin et al Cell. 2011 Sep 16;146(6):955-68.

   Type

   Empty backbone

   Tags

   Dendra2 PAFP, S-peptide, and TEV protease cleavag…

   Expression

   Worm

   Promoter

   pie-1

   Available Since

   Aug. 24, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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3. pPD162.21

   Plasmid

   #27511

   Depositor

   Andrew Fire

   Article

   Zhang et al Dev Biol. 2010 Aug 15. 344(2):603-10.

   Insert

   pmyo-3::dsRed::let-858_3'UTR
   

   Expression

   Worm

   Available Since

   Feb. 24, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGC76

   Plasmid

   #19641

   Depositor

   Jane Hubbard

   Article

   Voutev et al Genetics. 2008 Sep;180(1):103-19. doi: 1

   Insert

   <4xNLS-GFP::let-858(3’)
   

   Use

   Flp/frt

   Tags

   4xNLS

   Expression

   Worm

   Available Since

   March 16, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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5. pPD95_02

   Plasmid

   #1478

   Depositor

   Andrew Fire

   Type

   Empty backbone

   Tags

   T3p, gfpN, IVSR, gfpS65C, IVSS, IVST, gfpC, T7p, …

   Expression

   Worm

   Available Since

   March 7, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMS74 (synthetic guide::∆HYGR::unc-54 3' UTR^SEC)

   Plasmid

   #154838

   Purpose

   Insertion of split hygromycin landing pad into ChrII:8420157 site C. elegans strains via CRISPR with SEC selection

   Depositor

   Patrick Phillips

   Article

   Stevenson et al G3 (Bethesda). 2020 Aug 19. pii: g3.120.

   Insert

   synthetic guide::∆HYGR::unc-54 3' UTR
   

   Use

   CRISPR and Cre/Lox

   Expression

   Worm

   Mutation

   ∆HYGR is promoterless and encodes aa 60-341

   Available Since

   Aug. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJH3666

   Plasmid

   #191447

   Purpose

   Pacr-2s-GCaMP6s::wCherry unc-54 3' UTR C.elegans A/B MN expression of GCaMP6s RFP

   Depositor

   Mei Zhen

   Article

   Lu et al Curr Biol. 2022 Nov 7;32(21):4631-4644.e

   Insert

   GCaMP6s
   

   Tags

   wCherry

   Expression

   Worm

   Promoter

   Pacr-2s

   Available Since

   Dec. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJH3043

   Plasmid

   #175284

   Purpose

   Pdpy-30 DAF-16a unc-54 3' UTR C.elegans pan-tissue expression of isoform a of DAF-16 fused to GFP

   Depositor

   Mei Zhen

   Article

   Hung et al Development. 2014 Apr;141(8):1767-79. do

   Insert

   daf-16a mini-gene (daf-16 Nematode)
   

   Expression

   Worm

   Promoter

   Pdpy-30

   Available Since

   Nov. 10, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

9. L4663

   Plasmid

   #1665

   Depositor

   Andrew Fire

   Type

   Empty backbone

   Tags

   myo3-2341, myo3Tsa, SV40NLS, gfpN, IVSR, gfpY66W,…

   Expression

   Worm

   Available Since

   March 7, 2005

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pHA843 - hrp-1p::hrp-1HsLCdeltaLC

    Plasmid

    #139200

    Purpose

    Expresses hrp-1p::hrp-1HsLCdeltaLC::hrp-1 3'UTR

    Depositor

    Anne Hart

    Article

    Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

    Insert

    hrp-1 (hrp-1 Nematode)
    

    Expression

    Worm

    Mutation

    stop at beginning of last exon

    Available Since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEY68

    Plasmid

    #191065

    Purpose

    ceh-14 fluorescent neural reporter driving nuclear mNeptune2.5 expression (refer to NeuroPAL paper for expression)

    Depositor

    Eviatar Yemini, Oliver Hobert

    Article

    Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

    Insert

    ceh-14 (ceh-14 Nematode)
    

    Expression

    Worm

    Mutation

    None

    Available Since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pEY61

    Plasmid

    #191058

    Purpose

    acr-2(3.7k) fluorescent neural reporter driving nuclear mNeptune2.5 expression (refer to NeuroPAL paper for expression)

    Depositor

    Eviatar Yemini, Oliver Hobert

    Article

    Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

    Insert

    acr-2(3.7k) (acr-2 Nematode)
    

    Expression

    Worm

    Mutation

    None

    Available Since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. L4404

    Plasmid

    #1648

    Depositor

    Andrew Fire

    Type

    Empty backbone

    Use

    RNAi

    Tags

    T7p, lacZN, OriF1>>, OriF1<<, T3p

    Expression

    Worm

    Available Since

    March 7, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. L4400

    Plasmid

    #1647

    Depositor

    Andrew Fire

    Type

    Empty backbone

    Use

    RNAi

    Tags

    OriF1<<, OriF1>>, lacZN, T7p, unc2213…

    Expression

    Worm

    Available Since

    March 7, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCM5.50

    Plasmid

    #19383

    Depositor

    Geraldine Seydoux

    Article

    Merritt et al Curr Biol. 2008 Oct 14;18(19):1476-82. d

    Insert

    spn-4 3'utr in pDONRP2RP3 (spn-4 Nematode)
    

    Expression

    Worm

    Available Since

    Oct. 1, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. AP568-3

    Plasmid

    #70048

    Purpose

    co-expression of Cas9 and crRNA 589 target sequence

    Depositor

    Geraldine Seydoux

    Article

    Paix et al Genetics. 2015 Sep;201(1):47-54. doi: 10

    Insert

    sgRNA for dpy‐10
    

    Use

    CRISPR

    Expression

    Worm

    Available Since

    Nov. 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. nud-1::L4440

    Plasmid

    #11337

    Depositor

    Guy Caldwell

    Insert

    nud-1 (nud-1 Nematode)
    

    Use

    RNAi

    Expression

    Worm

    Available Since

    July 12, 2006

    Availability

    Academic Institutions and Nonprofits only

18. pAS1-his-24::tagRFP

    Plasmid

    #124329

    Purpose

    Expressing Nuclear Localized tagRFP

    Depositor

    Andrew Leifer

    Insert

    tagRFP (his-24 Nematode)
    

    Expression

    Worm

    Available Since

    April 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pGC133

    Plasmid

    #19612

    Depositor

    Jane Hubbard

    Article

    Voutev et al Genetics. 2008 Sep;180(1):103-19. doi: 1

    Insert

    Phsp-16.41::FLP::let-858(3’)-(Cbr)unc-119(+)
    

    Use

    Flp/frt

    Expression

    Worm

    Available Since

    March 5, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pJW1882

    Plasmid

    #154330

    Purpose

    sgRNA Target Vector for CRISPR

    Depositor

    Jordan Ward

    Article

    Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

    Insert

    ttTi4348 site targeting sgRNA (F+E) with R07E5.16 U6 promoter and 3'UTR
    

    Use

    CRISPR

    Expression

    Worm

    Mutation

    F+E mutations in sgRNA

    Available Since

    Dec. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pCM1.111

    Plasmid

    #19598

    Depositor

    Geraldine Seydoux

    Insert

    mex-5 prom in PDONRP4P1R
    

    Expression

    Worm

    Available Since

    Nov. 10, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pCS2+ oDam_f_GFPoNLS

    Plasmid

    #85819

    Purpose

    iDamID plasmid. To express transiently the optimized E. coli Dam adenine methyltransferase fused to the nuclear-localized mmGFP via flexylinker.

    Depositor

    Joachim Wittbrodt

    Article

    Gutierrez-Triana et al Development. 2016 Oct 5. pii: dev.139261

    Insert

    oDam_f_GFPoNLS
    

    Tags

    oNLS (optimized nuclear localization signal) C te…

    Expression

    Bacterial, Insect, Mammalia…

    Mutation

    L122A. Codon optimization compatible to work in M…

    Available Since

    Jan. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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23. pSEM386 - mosTI unc-119 - NNN - gfp - tbb-2

    Plasmid

    #182345

    Purpose

    GFP fusion cloning vector for mosTI(unc-119). Transgene inserted upstream of gfp and tbb-2 3' UTR. MCS or Golden Gate (BsaI: tgcc - MCS - aaaa)

    Depositor

    Christian Froekjaer-Jensen

    Article

    El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

    Type

    Empty backbone

    Expression

    Worm

    Available Since

    May 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCG26

    Plasmid

    #26938

    Depositor

    Geraldine Seydoux

    Article

    Gallo et al Dev Biol. 2008 Nov 1. 323(1):76-87.

    Insert

    dcap-1 (dcap-1 Nematode)
    

    Tags

    GFP

    Expression

    Worm

    Available Since

    May 11, 2011

    Availability

    Academic Institutions and Nonprofits only

25. L4640

    Plasmid

    #1662

    Depositor

    Andrew Fire

    Type

    Empty backbone

    Tags

    myo2-1090, myo25', IVSA, rf2128, gfpN, IVSR,…

    Expression

    Worm

    Available Since

    March 7, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pJN601

    Plasmid

    #59790

    Purpose

    zf1::yfp fusion with floxed unc-119(+) within an intron of yfp. For creating zf1::yfp knock-ins using unc-119 as a marker

    Depositor

    Jeremy Nance

    Article

    Armenti et al Development. 2014 Dec;141(23):4640-7. do

    Inserts

    ZF1 domain from pie-1
    N-terminal half of yfp with introns
    intron containing floxed unc-119 gene in reverse orientation
    C-terminal half of yfp with introns
    

    Expression

    Worm

    Available Since

    Oct. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. L3135

    Plasmid

    #1531

    Depositor

    Andrew Fire

    Type

    Empty backbone

    Tags

    IVSA, ATG-SecSig, pes10

    Expression

    Worm

    Available Since

    March 7, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pJH2947

    Plasmid

    #132362

    Purpose

    Prgef-1 INS-2::GFP unc-54 3' UTR C.elegans pan-neural expression of INS-2::GFP

    Depositor

    Mei Zhen

    Article

    Hung et al Development. 2014 Apr;141(8):1767-79. do

    Insert

    INS-2 (ins-2 Nematode)
    

    Tags

    GFP

    Expression

    Worm

    Promoter

    Prgef-1

    Available Since

    Oct. 7, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

29. pCM5.44

    Plasmid

    #19389

    Depositor

    Geraldine Seydoux

    Article

    Merritt et al Curr Biol. 2008 Oct 14;18(19):1476-82. d

    Insert

    cep-1 3'utr in pDONRP2RP3
    

    Expression

    Worm

    Available Since

    Nov. 10, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pRG02

    Plasmid

    #44196

    Depositor

    Denis Dupuy

    Insert

    Neo
    

    Expression

    Worm

    Promoter

    rps-27

    Available Since

    April 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge